{"id":"https://openalex.org/W2012847227","doi":"https://doi.org/10.1073/pnas.80.5.1194","title":"Efficient isolation of genes by using antibody probes.","display_name":"Efficient isolation of genes by using antibody probes.","publication_year":1983,"publication_date":"1983-03-01","ids":{"openalex":"https://openalex.org/W2012847227","doi":"https://doi.org/10.1073/pnas.80.5.1194","mag":"2012847227","pmid":"https://pubmed.ncbi.nlm.nih.gov/6219389","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/393560"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.80.5.1194","doi":"https://doi.org/10.1073/pnas.80.5.1194","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.80.5.1194","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/393560","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112973354","display_name":"R A Young","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210137306","display_name":"Stanford Medicine","ror":"https://ror.org/03mtd9a03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210137306","https://openalex.org/I97018004"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R A Young","raw_affiliation_strings":["Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305","institution_ids":["https://openalex.org/I4210137306","https://openalex.org/I97018004"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022830611","display_name":"Ronald Wayne Davis","orcid":"https://orcid.org/0000-0003-3123-2333","observed_orcids":["https://orcid.org/0000-0003-3123-2333"]},"institutions":[{"id":"https://openalex.org/I4210137306","display_name":"Stanford Medicine","ror":"https://ror.org/03mtd9a03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210137306","https://openalex.org/I97018004"]},{"id":"https://openalex.org/I97018004","display_name":"Stanford University","ror":"https://ror.org/00f54p054","country_code":"US","type":"education","lineage":["https://openalex.org/I97018004"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R W Davis","raw_affiliation_strings":["Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305","institution_ids":["https://openalex.org/I4210137306","https://openalex.org/I97018004"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":97.632,"has_fulltext":false,"cited_by_count":2017,"citation_normalized_percentile":{"value":0.99984348,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"80","issue":"5","first_page":"1194","last_page":"1198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.4787999987602234,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.4787999987602234,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.25850000977516174,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.15369999408721924,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antibody-probes","display_name":"antibody probes","score":0.6800000071525574},{"id":"https://openalex.org/keywords/gene-isolation","display_name":"gene isolation","score":0.7760000228881836},{"id":"https://openalex.org/keywords/lacz-fusion","display_name":"lacZ fusions","score":0.5979999899864197},{"id":"https://openalex.org/keywords/antigen-screening","display_name":"antigen screening","score":0.49900001287460327},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.7839999794960022},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.3880000114440918}],"concepts":[{"id":"https://openalex.org/C48292096","wikidata":"https://www.wikidata.org/wiki/Q6710264","display_name":"Lysogen","level":5,"score":0.8939533233642578},{"id":"https://openalex.org/C136199206","wikidata":"https://www.wikidata.org/wiki/Q1148110","display_name":"Lysogenic cycle","level":5,"score":0.7917876243591309},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6583585143089294},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6037267446517944},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.584558367729187},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5778115391731262},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5675270557403564},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.5585228800773621},{"id":"https://openalex.org/C2781135666","wikidata":"https://www.wikidata.org/wiki/Q615868","display_name":"Lambda phage","level":5,"score":0.5494763255119324},{"id":"https://openalex.org/C168374021","wikidata":"https://www.wikidata.org/wiki/Q1924179","display_name":"Cloning vector","level":5,"score":0.54158616065979},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.5320025086402893},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4707917273044586},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.46013277769088745},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.4575587213039398},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.45381540060043335},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.447797954082489},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.44110748171806335},{"id":"https://openalex.org/C10079403","wikidata":"https://www.wikidata.org/wiki/Q424475","display_name":"Phagemid","level":5,"score":0.42187976837158203},{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.4216388165950775},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.41458287835121155},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2719123959541321},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.19618567824363708},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D005814","descriptor_name":"Genes, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008242","descriptor_name":"Lysogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010582","descriptor_name":"Bacteriophage lambda","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010582","descriptor_name":"Bacteriophage lambda","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1073/pnas.80.5.1194","doi":"https://doi.org/10.1073/pnas.80.5.1194","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.80.5.1194","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:393560","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/393560","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmid:6219389","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6219389","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"mag:2289166582","doi":null,"is_oa":false,"landing_page_url":"http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202010%20710.LC/Lecture%206%20reading/Lambda%20gt11.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:2518297063","doi":null,"is_oa":false,"landing_page_url":"https://www.jstor.org/stable/pdfplus/13535.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:393560","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/393560","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W318334032","https://openalex.org/W1528427127","https://openalex.org/W1546783991","https://openalex.org/W1976507877","https://openalex.org/W1981188561","https://openalex.org/W1982584779","https://openalex.org/W1986299132","https://openalex.org/W1995013658","https://openalex.org/W2012876477","https://openalex.org/W2012880496","https://openalex.org/W2015963053","https://openalex.org/W2018182039","https://openalex.org/W2018289835","https://openalex.org/W2022579820","https://openalex.org/W2034149051","https://openalex.org/W2034347319","https://openalex.org/W2050283738","https://openalex.org/W2053826685","https://openalex.org/W2054878560","https://openalex.org/W2081496237","https://openalex.org/W2093535437","https://openalex.org/W2095413803","https://openalex.org/W2100837269","https://openalex.org/W2154251996","https://openalex.org/W2155635337","https://openalex.org/W2164253976","https://openalex.org/W2180080905"],"related_works":["https://openalex.org/W2518297063","https://openalex.org/W1534139725","https://openalex.org/W959241171","https://openalex.org/W271505767","https://openalex.org/W3026185639","https://openalex.org/W2409267114","https://openalex.org/W2049295776","https://openalex.org/W4388362727","https://openalex.org/W19822702","https://openalex.org/W952523643"],"abstract_inverted_index":{"A":[0],"sensitive":[1],"and":[2,20,52],"general":[3],"technique":[4],"has":[5],"been":[6],"devised":[7],"for":[8],"the":[9,47,74,108,142,145,158],"dual":[10],"purposes":[11],"of":[12,43,55,60,73,83,90,110,144],"cloning":[13],"genes":[14],"by":[15,25,113,121,148],"using":[16],"antibodies":[17],"as":[18],"probes":[19],"isolating":[21],"unknown":[22],"proteins":[23,111,119],"encoded":[24,120,147],"cloned":[26,115],"DNA.":[27],"The":[28,101],"method":[29],"uses":[30],"an":[31],"expression":[32],"vector,":[33],"lambda":[34,64],"gt11":[35,65],"(lac5":[36],"nin5":[37],"cI857":[38],"S100),":[39],"that":[40],"permits":[41],"insertion":[42],"foreign":[44,149],"DNA":[45,150],"into":[46],"beta-galactosidase":[48],"structural":[49],"gene":[50,116],"lacZ":[51],"promotes":[53],"synthesis":[54],"hybrid":[56,146],"proteins.":[57],"Efficient":[58],"screening":[59],"antigen-producing":[61],"clones":[62],"in":[63,77,125,128,133,152],"recombinant":[66,122],"cDNA":[67],"libraries":[68],"is":[69,103],"achieved":[70],"through":[71],"lysogeny":[72],"phage":[75,123],"library":[76],"hflA":[78],"(high-frequency":[79],"lysogeny)":[80],"mutant":[81],"cells":[82],"Escherichia":[84],"coli;":[85],"lysogens":[86],"produce":[87],"detectable":[88],"quantities":[89],"antigen":[91],"on":[92],"induction,":[93],"even":[94],"when":[95],"plated":[96],"at":[97],"high":[98],"cell":[99],"densities.":[100],"vector":[102],"also":[104],"designed":[105],"to":[106,136,156],"facilitate":[107],"isolation":[109],"specified":[112],"previously":[114],"sequences.":[117],"Hybrid":[118],"accumulate":[124],"strains":[126],"defective":[127],"protein":[129],"degradation":[130],"(lon":[131],"mutants)":[132],"amounts":[134],"amenable":[135],"large-scale":[137],"purification.":[138],"Antibodies":[139],"produced":[140],"against":[141],"portion":[143],"could":[151],"turn":[153],"be":[154],"used":[155],"isolate":[157],"native":[159],"polypeptide":[160],"from":[161],"eukaryotic":[162],"cells.":[163]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
