{"id":"https://openalex.org/W2012777643","doi":"https://doi.org/10.1016/j.reprotox.2003.10.015","title":"Improvement of an in vitro stem cell assay for developmental toxicity: the use of molecular endpoints in the embryonic stem cell test","display_name":"Improvement of an in vitro stem cell assay for developmental toxicity: the use of molecular endpoints in the embryonic stem cell test","publication_year":2004,"publication_date":"2004-02-11","ids":{"openalex":"https://openalex.org/W2012777643","doi":"https://doi.org/10.1016/j.reprotox.2003.10.015","mag":"2012777643","pmid":"https://pubmed.ncbi.nlm.nih.gov/15019721"},"language":"en","primary_location":{"id":"doi:10.1016/j.reprotox.2003.10.015","doi":"https://doi.org/10.1016/j.reprotox.2003.10.015","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.reprotox.2003.10.015","pdf_url":null,"source":{"id":"https://openalex.org/S1879512","display_name":"Reproductive Toxicology","issn_l":"0890-6238","issn":["0890-6238","1873-1708"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reproductive Toxicology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071251888","display_name":"Andrea E. M. Seiler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801133538","display_name":"German Federal Institute for Risk Assessment (BfR)","ror":"https://ror.org/03k3ky186","country_code":"DE","type":"government","lineage":["https://openalex.org/I2801133538","https://openalex.org/I2801963157"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Andrea Seiler","raw_affiliation_strings":["National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany. seiler.zebet@bfr.bund.de","National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany. seiler.zebet@bfr.bund.de","institution_ids":["https://openalex.org/I2801133538"]},{"raw_affiliation_string":"National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany","institution_ids":["https://openalex.org/I2801133538"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056144496","display_name":"A. Visan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801133538","display_name":"German Federal Institute for Risk Assessment (BfR)","ror":"https://ror.org/03k3ky186","country_code":"DE","type":"government","lineage":["https://openalex.org/I2801133538","https://openalex.org/I2801963157"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Anke Visan","raw_affiliation_strings":["National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany","institution_ids":["https://openalex.org/I2801133538"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004679639","display_name":"Roland Buesen","orcid":"https://orcid.org/0000-0002-6531-1200","observed_orcids":["https://orcid.org/0000-0002-6531-1200"]},"institutions":[{"id":"https://openalex.org/I2801133538","display_name":"German Federal Institute for Risk Assessment (BfR)","ror":"https://ror.org/03k3ky186","country_code":"DE","type":"government","lineage":["https://openalex.org/I2801133538","https://openalex.org/I2801963157"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Roland Buesen","raw_affiliation_strings":["National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany","institution_ids":["https://openalex.org/I2801133538"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034024376","display_name":"Elke Genschow","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801133538","display_name":"German Federal Institute for Risk Assessment (BfR)","ror":"https://ror.org/03k3ky186","country_code":"DE","type":"government","lineage":["https://openalex.org/I2801133538","https://openalex.org/I2801963157"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Elke Genschow","raw_affiliation_strings":["National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany","institution_ids":["https://openalex.org/I2801133538"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079990086","display_name":"Horst Spielmann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801133538","display_name":"German Federal Institute for Risk Assessment (BfR)","ror":"https://ror.org/03k3ky186","country_code":"DE","type":"government","lineage":["https://openalex.org/I2801133538","https://openalex.org/I2801963157"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Horst Spielmann","raw_affiliation_strings":["National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Center for Documentation and Evaluation of Alternative Methods to Animal Experiments (ZEBET), Federal Institute for Risk Assessment (BfR), Berlin 12277, Germany","institution_ids":["https://openalex.org/I2801133538"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5071251888"],"corresponding_institution_ids":["https://openalex.org/I2801133538"],"apc_list":{"value":3800,"currency":"USD","value_usd":3800},"apc_paid":null,"fwci":15.0509,"has_fulltext":false,"cited_by_count":189,"citation_normalized_percentile":{"value":0.99599434,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":100},"biblio":{"volume":"18","issue":"2","first_page":"231","last_page":"240"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12281","display_name":"Animal testing and alternatives","score":0.7085000276565552,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12281","display_name":"Animal testing and alternatives","score":0.7085000276565552,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.2761000096797943,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/embryonic-stem-cell-test","display_name":"embryonic stem cell test","score":0.9980000257492065},{"id":"https://openalex.org/keywords/developmental-toxicity","display_name":"developmental toxicity","score":0.8360000252723694},{"id":"https://openalex.org/keywords/embryotoxicity","display_name":"embryotoxicity","score":0.9079999923706055},{"id":"https://openalex.org/keywords/molecular-endpoints","display_name":"molecular endpoints","score":0.5789999961853027},{"id":"https://openalex.org/keywords/alpha-actinin","display_name":"alpha-actinin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/cardiomyocyte-differentiation","display_name":"cardiomyocyte differentiation","score":0.9200000166893005},{"id":"https://openalex.org/keywords/embryoid-bodies","display_name":"embryoid bodies","score":0.8370000123977661},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7929999828338623},{"id":"https://openalex.org/keywords/teratogenicity","display_name":"teratogenicity","score":0.8080000281333923},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.5759999752044678},{"id":"https://openalex.org/keywords/quantitative-gene-expression","display_name":"quantitative gene expression","score":0.5490000247955322},{"id":"https://openalex.org/keywords/risk-assessment","display_name":"risk assessment","score":0.5870000123977661}],"concepts":[{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.6617739200592041},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6314966678619385},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6004434823989868},{"id":"https://openalex.org/C6997183","wikidata":"https://www.wikidata.org/wiki/Q271727","display_name":"Myosin","level":2,"score":0.581338107585907},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.5598092675209045},{"id":"https://openalex.org/C143948831","wikidata":"https://www.wikidata.org/wiki/Q3384920","display_name":"Embryoid body","level":5,"score":0.517859935760498},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.5091802477836609},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5064607858657837},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.4881497323513031},{"id":"https://openalex.org/C179335377","wikidata":"https://www.wikidata.org/wiki/Q5266825","display_name":"Developmental toxicity","level":4,"score":0.4589574933052063},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.43370765447616577},{"id":"https://openalex.org/C136302470","wikidata":"https://www.wikidata.org/wiki/Q7607299","display_name":"Stem cell marker","level":3,"score":0.42530521750450134},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35758790373802185},{"id":"https://openalex.org/C20875687","wikidata":"https://www.wikidata.org/wiki/Q2642600","display_name":"Adult stem cell","level":4,"score":0.19708263874053955},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1649438440799713},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11230814456939697},{"id":"https://openalex.org/C172680121","wikidata":"https://www.wikidata.org/wiki/Q26513","display_name":"Fetus","level":3,"score":0.08008664846420288},{"id":"https://openalex.org/C2779234561","wikidata":"https://www.wikidata.org/wiki/Q11995","display_name":"Pregnancy","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000185","descriptor_name":"Actinin","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000185","descriptor_name":"Actinin","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003114","descriptor_name":"Colony-Forming Units Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004622","descriptor_name":"Embryo, Mammalian","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D004622","descriptor_name":"Embryo, Mammalian","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005472","descriptor_name":"Fluorouracil","qualifier_ui":"Q000633","qualifier_name":"toxicity","is_major_topic":false},{"descriptor_ui":"D005544","descriptor_name":"Forecasting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012518","descriptor_name":"Sarcomeres","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D012518","descriptor_name":"Sarcomeres","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013234","descriptor_name":"Stem Cells","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D013234","descriptor_name":"Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013723","descriptor_name":"Teratogens","qualifier_ui":"Q000633","qualifier_name":"toxicity","is_major_topic":true},{"descriptor_ui":"D014212","descriptor_name":"Tretinoin","qualifier_ui":"Q000633","qualifier_name":"toxicity","is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D018995","descriptor_name":"Myosin Heavy Chains","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D018995","descriptor_name":"Myosin Heavy Chains","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D032383","descriptor_name":"Myocytes, Cardiac","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D041702","descriptor_name":"BALB 3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.reprotox.2003.10.015","doi":"https://doi.org/10.1016/j.reprotox.2003.10.015","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.reprotox.2003.10.015","pdf_url":null,"source":{"id":"https://openalex.org/S1879512","display_name":"Reproductive Toxicology","issn_l":"0890-6238","issn":["0890-6238","1873-1708"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reproductive Toxicology","raw_type":"journal-article"},{"id":"pmid:15019721","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15019721","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Reproductive toxicology (Elmsford, N.Y.)","raw_type":"Comparative Study"},{"id":"pmh:oai:www.openagrar.de:bimport_mods_00000329","doi":null,"is_oa":false,"landing_page_url":"https://www.openagrar.de/receive/bimport_mods_00000329","pdf_url":null,"source":{"id":"https://openalex.org/S7407055137","display_name":"OpenAgrar","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W27814405","https://openalex.org/W32541466","https://openalex.org/W79462420","https://openalex.org/W194388979","https://openalex.org/W1948275303","https://openalex.org/W1948938001","https://openalex.org/W1992987605","https://openalex.org/W1997427091","https://openalex.org/W2026419792","https://openalex.org/W2031283945","https://openalex.org/W2039651932","https://openalex.org/W2043575567","https://openalex.org/W2051395019","https://openalex.org/W2066758609","https://openalex.org/W2104836535","https://openalex.org/W2123308898","https://openalex.org/W2136071912","https://openalex.org/W2165622092","https://openalex.org/W2170560537","https://openalex.org/W2217643654","https://openalex.org/W2288848833","https://openalex.org/W2314777822","https://openalex.org/W2337949001","https://openalex.org/W2409346947","https://openalex.org/W2469014512","https://openalex.org/W4254175273","https://openalex.org/W6696412588"],"related_works":["https://openalex.org/W2088372846","https://openalex.org/W2400380266","https://openalex.org/W205674958","https://openalex.org/W2155016057","https://openalex.org/W2097383958","https://openalex.org/W2558092076","https://openalex.org/W2346493992","https://openalex.org/W1997427091","https://openalex.org/W1986813124","https://openalex.org/W2417176743"],"abstract_inverted_index":null,"counts_by_year":[{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":8},{"year":2015,"cited_by_count":9},{"year":2014,"cited_by_count":18},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":16}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}