{"id":"https://openalex.org/W2012769855","doi":"https://doi.org/10.1093/nar/17.24.10259","title":"High resolution footprinting ofEcoRI and distamycin with Rh(phi)2(bpy)3+, a new photofootprinting reagent","display_name":"High resolution footprinting ofEcoRI and distamycin with Rh(phi)2(bpy)3+, a new photofootprinting reagent","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W2012769855","doi":"https://doi.org/10.1093/nar/17.24.10259","mag":"2012769855","pmid":"https://pubmed.ncbi.nlm.nih.gov/2602152","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/335299"},"language":"en","primary_location":{"id":"doi:10.1093/nar/17.24.10259","doi":"https://doi.org/10.1093/nar/17.24.10259","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/17.24.10259","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/335299","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110969110","display_name":"Kiyoshi Uchida","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kiyoshi Uchida","raw_affiliation_strings":["Department of Chemistry, Columbia University, New York, NY 10027"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Columbia University, New York, NY 10027","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007909912","display_name":"Anna Marie Pyle","orcid":"https://orcid.org/0000-0001-9045-8872","observed_orcids":["https://orcid.org/0000-0001-9045-8872"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anna Marie Pyle","raw_affiliation_strings":["Department of Chemistry, Columbia University New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Columbia University New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067605795","display_name":"Takashi Morii","orcid":"https://orcid.org/0000-0003-3663-3267","observed_orcids":["https://orcid.org/0000-0003-3663-3267"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Takashi Morii","raw_affiliation_strings":["Department of Chemistry, Columbia University New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Columbia University New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5010855228","display_name":"Jacqueline K. Barton","orcid":"https://orcid.org/0000-0001-9883-1600","observed_orcids":["https://orcid.org/0000-0001-9883-1600"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jacqueline K. Barton","raw_affiliation_strings":["Department of Chemistry, Columbia University New York, NY 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Columbia University New York, NY 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5010855228"],"corresponding_institution_ids":["https://openalex.org/I78577930"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":1.2453,"has_fulltext":true,"cited_by_count":52,"citation_normalized_percentile":{"value":0.79379192,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"17","issue":"24","first_page":"10259","last_page":"10279"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.7020999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.7020999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10163","display_name":"Metal complexes synthesis and properties","score":0.24570000171661377,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13187","display_name":"Diffusion and Search Dynamics","score":0.01080000028014183,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rhodium-iii-complexes","display_name":"rhodium(III) complexes","score":0.8379999995231628},{"id":"https://openalex.org/keywords/ecori","display_name":"EcoRI","score":0.656000018119812},{"id":"https://openalex.org/keywords/distamycin","display_name":"distamycin","score":0.9959999918937683},{"id":"https://openalex.org/keywords/dna-binding-proteins","display_name":"DNA-binding proteins","score":0.5329999923706055},{"id":"https://openalex.org/keywords/dna-cleavage","display_name":"DNA cleavage","score":0.5329999923706055},{"id":"https://openalex.org/keywords/minor-groove-binding","display_name":"minor groove binding","score":0.6859999895095825},{"id":"https://openalex.org/keywords/major-groove-binding","display_name":"major groove binding","score":0.9819999933242798},{"id":"https://openalex.org/keywords/protein-dna-interactions","display_name":"protein-DNA interactions","score":0.44600000977516174}],"concepts":[{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.6007843613624573},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36007165908813477},{"id":"https://openalex.org/C74916050","wikidata":"https://www.wikidata.org/wiki/Q841090","display_name":"Classics","level":1,"score":0.32830750942230225},{"id":"https://openalex.org/C95457728","wikidata":"https://www.wikidata.org/wiki/Q309","display_name":"History","level":0,"score":0.2907102406024933},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1852523684501648}],"mesh":[{"descriptor_ui":"D001345","descriptor_name":"Autoradiography","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004214","descriptor_name":"Distamycins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007202","descriptor_name":"Indicators and Reagents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009942","descriptor_name":"Organometallic Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010777","descriptor_name":"Photochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011725","descriptor_name":"Pyridines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011758","descriptor_name":"Pyrroles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012238","descriptor_name":"Rhodium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014961","descriptor_name":"X-Ray Diffraction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015082","descriptor_name":"2,2'-Dipyridyl","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D015246","descriptor_name":"Deoxyribonuclease EcoRI","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/17.24.10259","doi":"https://doi.org/10.1093/nar/17.24.10259","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/17.24.10259","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:335299","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/335299","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:2602152","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2602152","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Comparative Study"},{"id":"pmh:oai:authors.library.caltech.edu:67080","doi":null,"is_oa":true,"landing_page_url":"https://resolver.caltech.edu/CaltechAUTHORS:20160513-121954121","pdf_url":"https://resolver.caltech.edu/CaltechAUTHORS:20160513-121954121","source":{"id":"https://openalex.org/S4306402161","display_name":"CaltechAUTHORS (California Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I122411786","host_organization_name":"California Institute of Technology","host_organization_lineage":["https://openalex.org/I122411786"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:335299","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/335299","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2402183966","display_name":null,"funder_award_id":"GM 07216","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7548229278","display_name":null,"funder_award_id":"GM33309","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320309398","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2012769855.pdf","grobid_xml":"https://content.openalex.org/works/W2012769855.grobid-xml"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W1513717105","https://openalex.org/W1556041268","https://openalex.org/W1558516755","https://openalex.org/W1575596796","https://openalex.org/W1788240279","https://openalex.org/W1963797060","https://openalex.org/W1967259286","https://openalex.org/W1968932980","https://openalex.org/W1989080074","https://openalex.org/W2001199233","https://openalex.org/W2005284350","https://openalex.org/W2006575684","https://openalex.org/W2010049199","https://openalex.org/W2012222517","https://openalex.org/W2013576475","https://openalex.org/W2016349058","https://openalex.org/W2017530901","https://openalex.org/W2022339605","https://openalex.org/W2022530111","https://openalex.org/W2022818332","https://openalex.org/W2024054329","https://openalex.org/W2036001656","https://openalex.org/W2037831135","https://openalex.org/W2037864120","https://openalex.org/W2037898928","https://openalex.org/W2045489285","https://openalex.org/W2050879305","https://openalex.org/W2051171991","https://openalex.org/W2057034095","https://openalex.org/W2064367068","https://openalex.org/W2068218858","https://openalex.org/W2074060550","https://openalex.org/W2079869065","https://openalex.org/W2086817292","https://openalex.org/W2165090578"],"related_works":["https://openalex.org/W2403736508","https://openalex.org/W2048710790","https://openalex.org/W2052446799","https://openalex.org/W2037864120","https://openalex.org/W2016746673","https://openalex.org/W1820729678","https://openalex.org/W1545558475","https://openalex.org/W160790829","https://openalex.org/W2012222517","https://openalex.org/W2952216917"],"abstract_inverted_index":{"The":[0,93],"complex":[1,142],"bis(phenanthrenequinone":[2],"diimine)(bipyridyl)rhodium(III),":[3],"Rh(phi)2(bpy)3+,":[4],"cleaves":[5],"DNA":[6,40],"efficiently":[7],"in":[8,41,51,163],"a":[9,18,33,57,71,101,107,144,177],"sequence-neutral":[10],"fashion":[11],"upon":[12],"photoactivation":[13],"so":[14],"as":[15,156,158,169],"to":[16,39,153],"provide":[17],"novel,":[19],"high":[20,102,150],"resolution,":[21,151],"chemical":[22],"photofootpring":[23],"reagent.":[24],"Photofootprinting":[25],"of":[26,120,129,146,166,174,189],"two":[27],"crystallographically":[28],"characterized":[29],"DNA-binding":[30,191],"agents,":[31,139],"distamycin,":[32],"small":[34],"natural":[35],"product":[36],"which":[37,49,122],"binds":[38,50],"the":[42,46,52,63,77,90,112,140,164,187,198],"minor":[43,159],"groove,":[44,54],"and":[45,70,111,176,196],"endonuclease":[47],"EcoRI,":[48],"major":[53,155],"gave":[55],"respectively":[56],"5-7":[58],"base":[59,73],"pair":[60,74],"footprint":[61,75,154],"for":[62,76,126],"drug":[64],"at":[65,80],"its":[66,81],"A6":[67],"binding":[68],"site":[69,83],"10-12":[72],"enzyme":[78],"centered":[79],"recognition":[82],"(5'-GAATTC-3').":[84],"Both":[85],"footprints":[86],"agree":[87],"closely":[88],"with":[89,131,135,193],"crystallographic":[91],"results.":[92],"photocleavage":[94],"reaction":[95],"can":[96],"be":[97],"performed":[98],"using":[99],"either":[100],"intensity":[103],"lamp":[104],"or,":[105],"conveniently,":[106],"simple":[108],"transilluminator":[109],"box,":[110],"photoreaction":[113],"is":[114],"not":[115],"inhibited":[116],"by":[117],"moderate":[118],"concentrations":[119],"reagents":[121],"are":[123],"sometimes":[124],"required":[125],"examining":[127,190],"interactions":[128,192],"molecules":[130],"DNA.":[132],"When":[133],"compared":[134],"other":[136],"popular":[137],"footprinting":[138,183],"rhodium":[141],"shows":[143],"number":[145],"distinct":[147],"advantages:":[148],"sequence-neutrality,":[149],"ability":[152],"well":[157],"groove-binding":[160],"ligands,":[161],"applicability":[162],"presence":[165],"additives":[167],"such":[168],"Mg2+":[170],"or":[171],"glycerol,":[172],"ease":[173],"handling,":[175],"sharply":[178],"footprinted":[179],"pattern.":[180],"Light":[181],"activated":[182],"reactions":[184],"furthermore":[185],"offer":[186],"possibility":[188],"time":[194],"resolution":[195],"within":[197],"cell.":[199]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}