{"id":"https://openalex.org/W2012507960","doi":"https://doi.org/10.1016/s0378-1097(03)00353-7","title":"Purification and properties of an esterase fromAspergillus nomiusHS-1 degrading ethylene glycol dibenzoate","display_name":"Purification and properties of an esterase fromAspergillus nomiusHS-1 degrading ethylene glycol dibenzoate","publication_year":2003,"publication_date":"2003-05-19","ids":{"openalex":"https://openalex.org/W2012507960","doi":"https://doi.org/10.1016/s0378-1097(03)00353-7","mag":"2012507960","pmid":"https://pubmed.ncbi.nlm.nih.gov/12799010"},"language":"en","primary_location":{"id":"doi:10.1016/s0378-1097(03)00353-7","doi":"https://doi.org/10.1016/s0378-1097(03)00353-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0378-1097(03)00353-7","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Hiroyuki Uchida","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111966504","display_name":"University of Fukui","ror":"https://ror.org/00msqp585","country_code":"JP","type":"education","lineage":["https://openalex.org/I111966504"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hiroyuki Uchida","raw_affiliation_strings":["Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi, 910-8507, Japan. uchida@acbio2.acbio.fukui-u.ac.jp","Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi, 910-8507, Japan. uchida@acbio2.acbio.fukui-u.ac.jp","institution_ids":["https://openalex.org/I111966504"]},{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan","institution_ids":["https://openalex.org/I111966504"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010958408","display_name":"Yuuma Kurakata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111966504","display_name":"University of Fukui","ror":"https://ror.org/00msqp585","country_code":"JP","type":"education","lineage":["https://openalex.org/I111966504"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuuma Kurakata","raw_affiliation_strings":["Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan","institution_ids":["https://openalex.org/I111966504"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066069803","display_name":"Hiroyuki Sawamura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111966504","display_name":"University of Fukui","ror":"https://ror.org/00msqp585","country_code":"JP","type":"education","lineage":["https://openalex.org/I111966504"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroyuki Sawamura","raw_affiliation_strings":["Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan","institution_ids":["https://openalex.org/I111966504"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078187140","display_name":"Norio Inamura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111966504","display_name":"University of Fukui","ror":"https://ror.org/00msqp585","country_code":"JP","type":"education","lineage":["https://openalex.org/I111966504"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Norio Inamura","raw_affiliation_strings":["Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan","institution_ids":["https://openalex.org/I111966504"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015362945","display_name":"Tomohiro Kotani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111966504","display_name":"University of Fukui","ror":"https://ror.org/00msqp585","country_code":"JP","type":"education","lineage":["https://openalex.org/I111966504"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tomohiro Kotani","raw_affiliation_strings":["Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan","institution_ids":["https://openalex.org/I111966504"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111693708","display_name":"Takayuki Uwajima","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111966504","display_name":"University of Fukui","ror":"https://ror.org/00msqp585","country_code":"JP","type":"education","lineage":["https://openalex.org/I111966504"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takayuki Uwajima","raw_affiliation_strings":["Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 9-1, Bunkyou 3-Chome, Fukui-shi 910-8507, Japan","institution_ids":["https://openalex.org/I111966504"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I111966504"],"apc_list":{"value":2500,"currency":"GBP","value_usd":3297},"apc_paid":null,"fwci":0.8324,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.63327769,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"223","issue":"1","first_page":"123","last_page":"127"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9189000129699707,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9189000129699707,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.05169999971985817,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.011800000444054604,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspergillus-nomius","display_name":"Aspergillus nomius","score":0.9049999713897705},{"id":"https://openalex.org/keywords/carboxylesterase","display_name":"carboxylesterase","score":0.9120000004768372},{"id":"https://openalex.org/keywords/ethyl-benzoate","display_name":"ethyl benzoate","score":0.9079999923706055},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8889999985694885},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.640999972820282},{"id":"https://openalex.org/keywords/p-nitrophenyl-esters","display_name":"p-nitrophenyl esters","score":0.8550000190734863},{"id":"https://openalex.org/keywords/phenylmethylsulfonyl-fluoride-inhibition","display_name":"phenylmethylsulfonyl fluoride inhibition","score":0.9139999747276306},{"id":"https://openalex.org/keywords/fungal-degradation","display_name":"fungal degradation","score":0.5}],"concepts":[{"id":"https://openalex.org/C2909400048","wikidata":"https://www.wikidata.org/wiki/Q411575","display_name":"Phenylmethylsulfonyl Fluoride","level":4,"score":0.8513785004615784},{"id":"https://openalex.org/C2777261171","wikidata":"https://www.wikidata.org/wiki/Q418750","display_name":"Esterase","level":3,"score":0.7585731744766235},{"id":"https://openalex.org/C2776753739","wikidata":"https://www.wikidata.org/wiki/Q407796","display_name":"Valerate","level":4,"score":0.7011288404464722},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.6919677257537842},{"id":"https://openalex.org/C2778036240","wikidata":"https://www.wikidata.org/wiki/Q421100","display_name":"Ethyl benzoate","level":3,"score":0.6282223463058472},{"id":"https://openalex.org/C2777516009","wikidata":"https://www.wikidata.org/wiki/Q194207","display_name":"Ethylene glycol","level":2,"score":0.5988209247589111},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5878344774246216},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.577497661113739},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5485202074050903},{"id":"https://openalex.org/C2778325069","wikidata":"https://www.wikidata.org/wiki/Q409840","display_name":"Carboxylesterase","level":3,"score":0.4874727725982666},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.40086066722869873},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3565858006477356},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.13109639286994934},{"id":"https://openalex.org/C2780865716","wikidata":"https://www.wikidata.org/wiki/Q411575","display_name":"PMSF","level":3,"score":0.10526072978973389},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.04823958873748779},{"id":"https://openalex.org/C2776979534","wikidata":"https://www.wikidata.org/wiki/Q193213","display_name":"Butyrate","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001565","descriptor_name":"Benzoates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004950","descriptor_name":"Esterases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004950","descriptor_name":"Esterases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004952","descriptor_name":"Esters","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005026","descriptor_name":"Ethylene Glycols","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012988","descriptor_name":"Soil Microbiology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0378-1097(03)00353-7","doi":"https://doi.org/10.1016/s0378-1097(03)00353-7","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0378-1097(03)00353-7","pdf_url":null,"source":{"id":"https://openalex.org/S34954451","display_name":"FEMS Microbiology Letters","issn_l":"0378-1097","issn":["0378-1097","1574-6968"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS Microbiology Letters","raw_type":"journal-article"},{"id":"pmid:12799010","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12799010","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"FEMS microbiology letters","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.990.864","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.990.864","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://femsle.oxfordjournals.org/content/femsle/223/1/123.full.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1765523660","https://openalex.org/W1966253955","https://openalex.org/W1982567828","https://openalex.org/W1998002890","https://openalex.org/W2000036344","https://openalex.org/W2014453929","https://openalex.org/W2022646757","https://openalex.org/W2026743918","https://openalex.org/W2027907532","https://openalex.org/W2032127084","https://openalex.org/W2034075465","https://openalex.org/W2042298854","https://openalex.org/W2063868099","https://openalex.org/W2083598389","https://openalex.org/W2097321270","https://openalex.org/W2100837269","https://openalex.org/W2105725793","https://openalex.org/W2131938552","https://openalex.org/W2166537396"],"related_works":["https://openalex.org/W7096214758","https://openalex.org/W2051067832","https://openalex.org/W2462406737","https://openalex.org/W7070949538","https://openalex.org/W136830244","https://openalex.org/W2490728743","https://openalex.org/W2055127733","https://openalex.org/W2561648583","https://openalex.org/W2010094084","https://openalex.org/W1910287433"],"abstract_inverted_index":{"We":[0],"isolated":[1],"a":[2,55,59],"fungal":[3],"strain":[4,46],"HS-1":[5],"utilizing":[6],"either":[7],"ethylene":[8],"glycol":[9],"dibenzoate":[10],"or":[11],"ethyl":[12],"benzoate":[13,79,94],"as":[14,24],"sole":[15],"source":[16],"of":[17,44,62],"carbon":[18],"and":[19,21,30,70,81,84,95],"energy,":[20],"identified":[22],"it":[23],"Aspergillus":[25],"nomius,":[26],"based":[27],"on":[28],"morphological":[29],"rDNA":[31],"analyses.":[32],"An":[33],"enzyme":[34,53,76],"hydrolyzing":[35],"the":[36,41,45,64,89],"esters":[37,80],"was":[38,54,67],"purified":[39],"from":[40],"culture":[42],"supernatant":[43],"to":[47],"an":[48],"electrophoretically":[49],"homogeneous":[50],"state.":[51],"The":[52,75],"carboxyl":[56],"esterase":[57],"with":[58],"monomeric":[60],"structure,":[61],"which":[63],"molecular":[65],"mass":[66],"about":[68],"60000,":[69],"inhibited":[71],"by":[72],"phenylmethylsulfonyl":[73],"fluoride.":[74],"hydrolyzed":[77],"various":[78],"p-nitrophenyl":[82,96],"esters,":[83],"its":[85],"hydrolysis":[86],"rates":[87],"for":[88],"most":[90],"favorable":[91],"substrates,":[92],"n-butyl":[93],"valerate,":[97],"were":[98],"comparable.":[99]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}