{"id":"https://openalex.org/W2011829144","doi":"https://doi.org/10.1016/0003-2697(90)90301-o","title":"A quantitative assay for neomycin phosphotransferase activity in plants","display_name":"A quantitative assay for neomycin phosphotransferase activity in plants","publication_year":1990,"publication_date":"1990-03-01","ids":{"openalex":"https://openalex.org/W2011829144","doi":"https://doi.org/10.1016/0003-2697(90)90301-o","mag":"2011829144","pmid":"https://pubmed.ncbi.nlm.nih.gov/2160205"},"language":"en","primary_location":{"id":"doi:10.1016/0003-2697(90)90301-o","doi":"https://doi.org/10.1016/0003-2697(90)90301-o","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(90)90301-o","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004510897","display_name":"Mark Staebell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark Staebell","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313","Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313","institution_ids":["https://openalex.org/I209244073"]},{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050669389","display_name":"Dwight T. Tomes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dwight Tomes","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109303222","display_name":"Arthur K. Weissinger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I137902535","display_name":"North Carolina State University","ror":"https://ror.org/04tj63d06","country_code":"US","type":"education","lineage":["https://openalex.org/I137902535"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Arthur Weissinger","raw_affiliation_strings":["Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695-7620 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695-7620 USA","institution_ids":["https://openalex.org/I137902535"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032322524","display_name":"Sheila Maddock","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sheila Maddock","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109226909","display_name":"Wallace A. Marsh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wallace Marsh","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041717688","display_name":"Gary A. Huffman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gary Huffman","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ronald Bauer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ronald Bauer","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015268276","display_name":"Margit Ross","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Margit Ross","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003338788","display_name":"John A. Howard","orcid":"https://orcid.org/0000-0002-5974-5416","observed_orcids":["https://orcid.org/0000-0002-5974-5416"]},"institutions":[{"id":"https://openalex.org/I209244073","display_name":"Pioneer Hi-Bred","ror":"https://ror.org/02nn4s922","country_code":"US","type":"company","lineage":["https://openalex.org/I209244073"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John Howard","raw_affiliation_strings":["Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pioneer Hi-Bred International, Inc., Department of Biotechnology Research, Johnston, Iowa 50313, USA","institution_ids":["https://openalex.org/I209244073"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":4.2904,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.93564703,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"185","issue":"2","first_page":"319","last_page":"323"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.5062999725341797,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.5062999725341797,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.37959998846054077,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.044599998742341995,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neomycin-phosphotransferase","display_name":"neomycin phosphotransferase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/enzyme-activity-assay","display_name":"enzyme activity assay","score":0.8399999737739563},{"id":"https://openalex.org/keywords/quantitative-assay","display_name":"quantitative assay","score":0.6169999837875366},{"id":"https://openalex.org/keywords/genetically-modified-plants","display_name":"transgenic plants","score":0.7850000262260437},{"id":"https://openalex.org/keywords/transient-expression","display_name":"transient expression","score":0.6039999723434448},{"id":"https://openalex.org/keywords/nicotiana-tabacum","display_name":"tobacco","score":0.7160000205039978},{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.8759999871253967},{"id":"https://openalex.org/keywords/desalination","display_name":"desalination","score":0.765999972820282},{"id":"https://openalex.org/keywords/extraction-buffer","display_name":"extraction buffer","score":0.6940000057220459}],"concepts":[{"id":"https://openalex.org/C2779841869","wikidata":"https://www.wikidata.org/wiki/Q423098","display_name":"Neomycin","level":3,"score":0.7236919403076172},{"id":"https://openalex.org/C86807702","wikidata":"https://www.wikidata.org/wiki/Q2620381","display_name":"Bovine serum albumin","level":2,"score":0.5957018136978149},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5884498953819275},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5809218883514404},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.5707281231880188},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5633995532989502},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5264081358909607},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.21001708507537842}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003313","descriptor_name":"Zea mays","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003313","descriptor_name":"Zea mays","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005966","descriptor_name":"Glucuronidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006041","descriptor_name":"Goats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008832","descriptor_name":"Microchemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D010047","descriptor_name":"Ovalbumin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010770","descriptor_name":"Phosphotransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010947","descriptor_name":"Plants, Toxic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011523","descriptor_name":"Protoplasts","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019868","descriptor_name":"Kanamycin Kinase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/0003-2697(90)90301-o","doi":"https://doi.org/10.1016/0003-2697(90)90301-o","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(90)90301-o","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:2160205","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2160205","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W1519899852","https://openalex.org/W1929815973","https://openalex.org/W1965900807","https://openalex.org/W1986587865","https://openalex.org/W1993993130","https://openalex.org/W2002875035","https://openalex.org/W2007885388","https://openalex.org/W2012019347","https://openalex.org/W2024556021","https://openalex.org/W2029196462","https://openalex.org/W2048290445","https://openalex.org/W2048365279","https://openalex.org/W2055584669","https://openalex.org/W2059804244","https://openalex.org/W2077545872","https://openalex.org/W2128635872"],"related_works":["https://openalex.org/W2069951506","https://openalex.org/W2065433789","https://openalex.org/W1965900807","https://openalex.org/W2029196462","https://openalex.org/W2074979978","https://openalex.org/W2012019347","https://openalex.org/W1986526857","https://openalex.org/W2091956835","https://openalex.org/W2086127516","https://openalex.org/W2036571046"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}