{"id":"https://openalex.org/W2011626631","doi":"https://doi.org/10.1016/s0003-9861(03)00004-3","title":"Isolation and characterization of 27-O-demethylrifamycin SV methyltransferase provides new insights into the post-PKS modification steps during the biosynthesis of the antitubercular drug rifamycin B by Amycolatopsis mediterranei S699","display_name":"Isolation and characterization of 27-O-demethylrifamycin SV methyltransferase provides new insights into the post-PKS modification steps during the biosynthesis of the antitubercular drug rifamycin B by Amycolatopsis mediterranei S699","publication_year":2003,"publication_date":"2003-03-01","ids":{"openalex":"https://openalex.org/W2011626631","doi":"https://doi.org/10.1016/s0003-9861(03)00004-3","mag":"2011626631","pmid":"https://pubmed.ncbi.nlm.nih.gov/12623077"},"language":"en","primary_location":{"id":"doi:10.1016/s0003-9861(03)00004-3","doi":"https://doi.org/10.1016/s0003-9861(03)00004-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0003-9861(03)00004-3","pdf_url":null,"source":{"id":"https://openalex.org/S153476765","display_name":"Archives of Biochemistry and Biophysics","issn_l":"0003-9861","issn":["0003-9861","1096-0384"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Biochemistry and Biophysics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112705270","display_name":"Jun Hui Xu","orcid":"https://orcid.org/0000-0002-3408-5896","observed_orcids":["https://orcid.org/0000-0002-3408-5896"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jun Xu","raw_affiliation_strings":["Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062053665","display_name":"Taifo Mahmud","orcid":"https://orcid.org/0000-0001-9639-526X","observed_orcids":["https://orcid.org/0000-0001-9639-526X"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Taifo Mahmud","raw_affiliation_strings":["Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027410094","display_name":"Heinz G. Floss","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Heinz G Floss","raw_affiliation_strings":["Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5062053665"],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":{"value":3170,"currency":"USD","value_usd":3170},"apc_paid":null,"fwci":1.0716,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.75754006,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"411","issue":"2","first_page":"277","last_page":"288"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13736","display_name":"Steroid Chemistry and Biochemistry","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12673","display_name":"Microbial Metabolism and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rifamycin-sv","display_name":"rifamycin SV","score":0.8730000257492065},{"id":"https://openalex.org/keywords/rifamycin-s","display_name":"rifamycin S","score":0.8230000138282776},{"id":"https://openalex.org/keywords/polyketide-synthase","display_name":"polyketide synthase","score":0.6039999723434448},{"id":"https://openalex.org/keywords/s-adenosylmethionine","display_name":"S-adenosylmethionine","score":0.8539999723434448},{"id":"https://openalex.org/keywords/enzyme-characterization","display_name":"enzyme characterization","score":0.550000011920929},{"id":"https://openalex.org/keywords/metal-ion-inhibition","display_name":"metal ion inhibition","score":0.847000002861023},{"id":"https://openalex.org/keywords/amycolatopsis-mediterranei","display_name":"Amycolatopsis mediterranei","score":0.8289999961853027},{"id":"https://openalex.org/keywords/antituberculosis-drugs","display_name":"antitubercular agents","score":0.6740000247955322}],"concepts":[{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.7123357653617859},{"id":"https://openalex.org/C2776306059","wikidata":"https://www.wikidata.org/wiki/Q388565","display_name":"Rifamycin","level":3,"score":0.669204592704773},{"id":"https://openalex.org/C91965660","wikidata":"https://www.wikidata.org/wiki/Q415875","display_name":"Methyltransferase","level":4,"score":0.5604490637779236},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5454578399658203},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.49511876702308655},{"id":"https://openalex.org/C2780887294","wikidata":"https://www.wikidata.org/wiki/Q7072037","display_name":"O-methyltransferase","level":5,"score":0.44711795449256897},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4272440969944},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3518219590187073},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.328498512506485},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.3236073851585388},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.29361391067504883},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.06505411863327026},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.052802830934524536}],"mesh":[{"descriptor_ui":"D000192","descriptor_name":"Actinomycetales","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000904","descriptor_name":"Antibiotics, Antitubercular","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008780","descriptor_name":"Methyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008780","descriptor_name":"Methyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012294","descriptor_name":"Rifamycins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D012294","descriptor_name":"Rifamycins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012294","descriptor_name":"Rifamycins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012436","descriptor_name":"S-Adenosylmethionine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0003-9861(03)00004-3","doi":"https://doi.org/10.1016/s0003-9861(03)00004-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0003-9861(03)00004-3","pdf_url":null,"source":{"id":"https://openalex.org/S153476765","display_name":"Archives of Biochemistry and Biophysics","issn_l":"0003-9861","issn":["0003-9861","1096-0384"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Biochemistry and Biophysics","raw_type":"journal-article"},{"id":"pmid:12623077","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12623077","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of biochemistry and biophysics","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1287679552","display_name":null,"funder_award_id":"AI-20264","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G4326945634","display_name":"BIOLOGICALLY ACTIVE MICROBIAL METABOLITES","funder_award_id":"R01AI020264","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320310094","display_name":"University of Washington","ror":"https://ror.org/00cvxb145"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":57,"referenced_works":["https://openalex.org/W425774368","https://openalex.org/W1541942572","https://openalex.org/W1590565022","https://openalex.org/W1608827347","https://openalex.org/W1672168756","https://openalex.org/W1832970157","https://openalex.org/W1863525853","https://openalex.org/W1955948793","https://openalex.org/W1970206241","https://openalex.org/W1978128362","https://openalex.org/W1978336624","https://openalex.org/W1986927066","https://openalex.org/W1989802754","https://openalex.org/W1990959599","https://openalex.org/W1994307142","https://openalex.org/W1994762770","https://openalex.org/W1995249676","https://openalex.org/W2008643214","https://openalex.org/W2022988477","https://openalex.org/W2024779852","https://openalex.org/W2024892153","https://openalex.org/W2045623837","https://openalex.org/W2047676419","https://openalex.org/W2052603675","https://openalex.org/W2061690278","https://openalex.org/W2063875833","https://openalex.org/W2064397676","https://openalex.org/W2066012786","https://openalex.org/W2066677629","https://openalex.org/W2068372433","https://openalex.org/W2071379525","https://openalex.org/W2078798579","https://openalex.org/W2082734817","https://openalex.org/W2085774588","https://openalex.org/W2093156299","https://openalex.org/W2097915721","https://openalex.org/W2104688428","https://openalex.org/W2117892897","https://openalex.org/W2121513555","https://openalex.org/W2122073065","https://openalex.org/W2123737628","https://openalex.org/W2130415821","https://openalex.org/W2144238576","https://openalex.org/W2144634347","https://openalex.org/W2147788336","https://openalex.org/W2149353640","https://openalex.org/W2149418879","https://openalex.org/W2153472178","https://openalex.org/W2161570550","https://openalex.org/W2164249947","https://openalex.org/W2184170742","https://openalex.org/W2314225431","https://openalex.org/W2322870763","https://openalex.org/W2396783373","https://openalex.org/W2477057870","https://openalex.org/W2952152151","https://openalex.org/W4295127969"],"related_works":["https://openalex.org/W2159444123","https://openalex.org/W2006082935","https://openalex.org/W2518709365","https://openalex.org/W3133722795","https://openalex.org/W3142165398","https://openalex.org/W2111628391","https://openalex.org/W2805110141","https://openalex.org/W4399979300","https://openalex.org/W1980779359","https://openalex.org/W2097915721"],"abstract_inverted_index":null,"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}