{"id":"https://openalex.org/W2011385454","doi":"https://doi.org/10.1021/bi900164d","title":"In Vitro Characterization of a Heterologously Expressed Nonribosomal Peptide Synthetase Involved in Phosphinothricin Tripeptide Biosynthesis","display_name":"In Vitro Characterization of a Heterologously Expressed Nonribosomal Peptide Synthetase Involved in Phosphinothricin Tripeptide Biosynthesis","publication_year":2009,"publication_date":"2009-05-11","ids":{"openalex":"https://openalex.org/W2011385454","doi":"https://doi.org/10.1021/bi900164d","mag":"2011385454","pmid":"https://pubmed.ncbi.nlm.nih.gov/19432442","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2709985"},"language":"en","primary_location":{"id":"doi:10.1021/bi900164d","doi":"https://doi.org/10.1021/bi900164d","is_oa":true,"landing_page_url":"https://doi.org/10.1021/bi900164d","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/bi900164d?ref=article_openPDF","source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://pubs.acs.org/doi/pdf/10.1021/bi900164d?ref=article_openPDF","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100610605","display_name":"Jin Hee Lee","orcid":"https://orcid.org/0000-0002-2385-2834","observed_orcids":["https://orcid.org/0000-0002-2385-2834","https://orcid.org/0009-0007-7015-6560"]},"institutions":[{"id":"https://openalex.org/I4405271866","display_name":"Carl R. Woese Institute for Genomic Biology","ror":"https://ror.org/036j6yj81","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405271866"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jin-Hee Lee","raw_affiliation_strings":["Department of Chemistry","Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry","institution_ids":[]},{"raw_affiliation_string":"Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I4405271866"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044740619","display_name":"Bradley S. Evans","orcid":"https://orcid.org/0000-0002-1207-9006","observed_orcids":["https://orcid.org/0000-0002-1207-9006"]},"institutions":[{"id":"https://openalex.org/I4405271866","display_name":"Carl R. Woese Institute for Genomic Biology","ror":"https://ror.org/036j6yj81","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405271866"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bradley S. Evans","raw_affiliation_strings":["Department of Biochemistry","Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry","institution_ids":[]},{"raw_affiliation_string":"Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I4405271866"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080916158","display_name":"Gongyong Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gongyong Li","raw_affiliation_strings":["Department of Chemistry","University of Illinois at Urbana Champaign"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry","institution_ids":[]},{"raw_affiliation_string":"University of Illinois at Urbana Champaign","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076257201","display_name":"Neil L. Kelleher","orcid":"https://orcid.org/0000-0002-8815-3372","observed_orcids":["https://orcid.org/0000-0002-8815-3372"]},"institutions":[{"id":"https://openalex.org/I4405271866","display_name":"Carl R. Woese Institute for Genomic Biology","ror":"https://ror.org/036j6yj81","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405271866"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Neil L. Kelleher","raw_affiliation_strings":["Department of Biochemistry","Department of Chemistry","Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry","institution_ids":[]},{"raw_affiliation_string":"Department of Chemistry","institution_ids":[]},{"raw_affiliation_string":"Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I4405271866"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067523147","display_name":"Wilfred A. van der Donk","orcid":"https://orcid.org/0000-0002-5467-7071","observed_orcids":["https://orcid.org/0000-0002-5467-7071"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I4405271866","display_name":"Carl R. Woese Institute for Genomic Biology","ror":"https://ror.org/036j6yj81","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405271866"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Wilfred A. van der Donk","raw_affiliation_strings":["Department of Biochemistry","Department of Chemistry","Howard Hughes Medical Institute","Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry","institution_ids":[]},{"raw_affiliation_string":"Department of Chemistry","institution_ids":[]},{"raw_affiliation_string":"Howard Hughes Medical Institute","institution_ids":["https://openalex.org/I1344073410"]},{"raw_affiliation_string":"Institute for Genomic Biology, 1206 West Gregory Drive, Urbana, Illinois 61801","institution_ids":["https://openalex.org/I4405271866"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5067523147","https://openalex.org/A5076257201"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I4405271866"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":{"value":4500,"currency":"USD","value_usd":4500},"fwci":1.6003,"has_fulltext":true,"cited_by_count":18,"citation_normalized_percentile":{"value":0.8057849,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"48","issue":"23","first_page":"5054","last_page":"5056"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nonribosomal-peptide-synthetase","display_name":"nonribosomal peptide synthetase","score":0.9829999804496765},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.9169999957084656},{"id":"https://openalex.org/keywords/thiolation-domain","display_name":"thiolation domain","score":0.859000027179718},{"id":"https://openalex.org/keywords/phosphopantetheine","display_name":"phosphopantetheine","score":0.9419999718666077},{"id":"https://openalex.org/keywords/peptide-biosynthesis","display_name":"peptide biosynthesis","score":0.5419999957084656},{"id":"https://openalex.org/keywords/streptomyces","display_name":"Streptomyces","score":0.6290000081062317}],"concepts":[{"id":"https://openalex.org/C167392928","wikidata":"https://www.wikidata.org/wiki/Q4820777","display_name":"Tripeptide","level":3,"score":0.8949214220046997},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.8808102011680603},{"id":"https://openalex.org/C2777379556","wikidata":"https://www.wikidata.org/wiki/Q1443801","display_name":"Nonribosomal peptide","level":4,"score":0.7705302238464355},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.6423119306564331},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.582558274269104},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5454656481742859},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4875726103782654},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.46680667996406555},{"id":"https://openalex.org/C2776043855","wikidata":"https://www.wikidata.org/wiki/Q55506489","display_name":"Pyrophosphate","level":3,"score":0.4556080400943756},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.44211965799331665},{"id":"https://openalex.org/C2780912031","wikidata":"https://www.wikidata.org/wiki/Q180341","display_name":"Methionine","level":3,"score":0.41210055351257324},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4066332280635834},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37422996759414673},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2622223496437073},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.21475234627723694},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.14246812462806702},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11726388335227966},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000613","descriptor_name":"Aminobutyrates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000613","descriptor_name":"Aminobutyrates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005583","descriptor_name":"Fourier Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009943","descriptor_name":"Organophosphorus Compounds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009943","descriptor_name":"Organophosphorus Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010452","descriptor_name":"Peptide Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010453","descriptor_name":"Peptide Synthases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010453","descriptor_name":"Peptide Synthases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011756","descriptor_name":"Diphosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011756","descriptor_name":"Diphosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1021/bi900164d","doi":"https://doi.org/10.1021/bi900164d","is_oa":true,"landing_page_url":"https://doi.org/10.1021/bi900164d","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/bi900164d?ref=article_openPDF","source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2709985","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2709985","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Text"},{"id":"pmid:19432442","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19432442","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.802.9138","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.802.9138","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/0f/f0/bi900164d.PMC2709985.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1021/bi900164d","doi":"https://doi.org/10.1021/bi900164d","is_oa":true,"landing_page_url":"https://doi.org/10.1021/bi900164d","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/bi900164d?ref=article_openPDF","source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1123170480","display_name":"An Institutional NRSA in Molecular Biophysics","funder_award_id":"T32GM008276","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G4057945511","display_name":"Discovery, Design, and Development of Phosphonic Acid Antibiotics","funder_award_id":"P01GM077596","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6558142369","display_name":null,"funder_award_id":"GM008276","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G9000204397","display_name":null,"funder_award_id":"PO1 GM077596","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000226354","display_name":null,"funder_award_id":"GM077596","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2011385454.pdf","grobid_xml":"https://content.openalex.org/works/W2011385454.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W1547799606","https://openalex.org/W1555618645","https://openalex.org/W1971802413","https://openalex.org/W1990783608","https://openalex.org/W2009414144","https://openalex.org/W2009471270","https://openalex.org/W2012162754","https://openalex.org/W2015514193","https://openalex.org/W2016238735","https://openalex.org/W2039878852","https://openalex.org/W2043753932","https://openalex.org/W2061634854","https://openalex.org/W2062552320","https://openalex.org/W2066483109","https://openalex.org/W2072449416","https://openalex.org/W2075551861","https://openalex.org/W2079015852","https://openalex.org/W2079692620","https://openalex.org/W2095215745","https://openalex.org/W2097854519","https://openalex.org/W2100137210","https://openalex.org/W2110474402","https://openalex.org/W2135261487","https://openalex.org/W2158730144","https://openalex.org/W2163094057","https://openalex.org/W2317181436"],"related_works":["https://openalex.org/W2110474402","https://openalex.org/W2202281839","https://openalex.org/W7097620784","https://openalex.org/W2163094057","https://openalex.org/W2015514193","https://openalex.org/W2012162754","https://openalex.org/W2317181436","https://openalex.org/W7095882498","https://openalex.org/W2024444541","https://openalex.org/W2415571552"],"abstract_inverted_index":{"The":[0,16,47],"late":[1],"stages":[2],"of":[3,5,19,40,87],"biosynthesis":[4],"phosphinothricin":[6],"tripeptide":[7],"(PTT)":[8],"involve":[9],"peptide":[10],"formation":[11],"and":[12],"methylation":[13],"on":[14],"phosphorus.":[15],"exact":[17],"timing":[18],"these":[20],"transformations":[21],"is":[22],"not":[23],"known.":[24],"To":[25],"provide":[26],"insight":[27],"into":[28],"this":[29],"question,":[30],"we":[31],"developed":[32],"a":[33],"heterologous":[34],"expression":[35],"system":[36],"for":[37,51,55,64],"PhsA,":[38],"one":[39],"three":[41],"NRPS":[42],"proteins":[43],"in":[44],"PTT":[45],"biosynthesis.":[46],"apparent":[48],"k(cat)/K(m)":[49],"value":[50],"ATP-pyrophosphate":[52],"exchange":[53],"activity":[54],"d,l-N-acetylphosphinothricin":[56],"was":[57,66],"3.5":[58],"muM(-1)":[59],"min(-1),":[60,69],"whereas":[61],"the":[62,71,75,84,88],"k(cat)/K(m,app)":[63],"l-N-acetyldemethylphosphinothricin":[65],"0.5":[67],"microM(-1)":[68],"suggesting":[70],"former":[72],"might":[73],"be":[74,81],"physiological":[76],"substrate.":[77],"Each":[78],"substrate":[79],"could":[80],"loaded":[82],"onto":[83],"phosphopantetheine":[85],"arm":[86],"thiolation":[89],"domain":[90],"as":[91],"observed":[92],"by":[93],"Fourier":[94],"transform":[95],"mass":[96],"spectrometry":[97],"(FTMS).":[98]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}