{"id":"https://openalex.org/W2011334735","doi":"https://doi.org/10.1021/bp060298i","title":"Accelerated Clone Selection for Recombinant CHO Cells Using a FACS\u2010Based High\u2010Throughput Screen","display_name":"Accelerated Clone Selection for Recombinant CHO Cells Using a FACS\u2010Based High\u2010Throughput Screen","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W2011334735","doi":"https://doi.org/10.1021/bp060298i","mag":"2011334735","pmid":"https://pubmed.ncbi.nlm.nih.gov/17261021"},"language":"en","primary_location":{"id":"doi:10.1021/bp060298i","doi":"https://doi.org/10.1021/bp060298i","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp060298i","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112368453","display_name":"Christine T. DeMaria","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Christine T. DeMaria","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701, USA. christine.demaria@genzyme.com","Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701, USA. christine.demaria@genzyme.com","institution_ids":[]},{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005815426","display_name":"Victor R. Cairns","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Victor Cairns","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085405469","display_name":"Cordula Schwarz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cordula Schwarz","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100405917","display_name":"Jin Zhang","orcid":"https://orcid.org/0000-0001-8738-2301","observed_orcids":["https://orcid.org/0000-0001-8738-2301"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jin Zhang","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034874542","display_name":"Megan Guerin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Megan Guerin","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032240804","display_name":"Erin Zuena","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Erin Zuena","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043022666","display_name":"Scott D. Estes","orcid":"https://orcid.org/0000-0003-1465-1034","observed_orcids":["https://orcid.org/0000-0003-1465-1034"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Scott Estes","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5033092571","display_name":"Kenneth P. Karey","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kenneth P. Karey","raw_affiliation_strings":["Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Therapeutic Protein Expression Group, Department of Cell & Protein Therapeutics, Genzyme Corporation, Framingham, Massachusetts 01701","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5112368453"],"corresponding_institution_ids":[],"apc_list":{"value":4160,"currency":"USD","value_usd":4160},"apc_paid":null,"fwci":1.5233,"has_fulltext":false,"cited_by_count":88,"citation_normalized_percentile":{"value":0.80494518,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"23","issue":"2","first_page":"465","last_page":"472"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/clone-selection","display_name":"clone selection","score":0.9079999923706055},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.7749999761581421},{"id":"https://openalex.org/keywords/therapeutic-protein-expression","display_name":"therapeutic protein expression","score":0.6620000004768372},{"id":"https://openalex.org/keywords/internal-ribosome-entry-site","display_name":"internal ribosome entry site","score":0.8199999928474426}],"concepts":[{"id":"https://openalex.org/C81089528","wikidata":"https://www.wikidata.org/wiki/Q5134986","display_name":"clone (Java method)","level":3,"score":0.8446592092514038},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.7248398065567017},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7097852230072021},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.6638545393943787},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.5564078092575073},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4609127640724182},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3643096685409546},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.360832542181015},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.33040621876716614},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20730137825012207},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2071639597415924},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18382272124290466},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.17037895321846008},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.09205034375190735},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0787833034992218},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003412","descriptor_name":"Cricetulus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D018951","descriptor_name":"Antigens, CD20","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1021/bp060298i","doi":"https://doi.org/10.1021/bp060298i","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bp060298i","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},{"id":"pmid:17261021","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17261021","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology progress","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.829.7002","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.829.7002","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.cytometry.cn/uploadfile/20073611403964.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.428600013256073}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W72481024","https://openalex.org/W1582733372","https://openalex.org/W1976492411","https://openalex.org/W1983182864","https://openalex.org/W1995562003","https://openalex.org/W2000313112","https://openalex.org/W2000853857","https://openalex.org/W2005343613","https://openalex.org/W2006554573","https://openalex.org/W2011432054","https://openalex.org/W2029545026","https://openalex.org/W2042216382","https://openalex.org/W2054773145","https://openalex.org/W2061273313","https://openalex.org/W2076918952","https://openalex.org/W2084618542","https://openalex.org/W2096628587","https://openalex.org/W2115124797","https://openalex.org/W2118388861","https://openalex.org/W2133398740","https://openalex.org/W2134820230","https://openalex.org/W2155218352","https://openalex.org/W2162470970","https://openalex.org/W2170615008"],"related_works":["https://openalex.org/W2396660618","https://openalex.org/W2014241095","https://openalex.org/W2150228251","https://openalex.org/W2128723663","https://openalex.org/W1589485362","https://openalex.org/W1983182864","https://openalex.org/W4402306300","https://openalex.org/W2006554573","https://openalex.org/W2076762497","https://openalex.org/W2133398740"],"abstract_inverted_index":{"Flow":[0],"cytometry":[1],"was":[2],"partnered":[3],"with":[4,38,120],"a":[5,20,36,45,82],"nonfluorescent":[6],"reporter":[7,53,86],"protein":[8,41,54,58,99,161],"for":[9,100,109,158],"rapid,":[10],"early":[11],"stage":[12,139],"identification":[13],"of":[14,19,96,117,123,133,154],"clones":[15,135],"producing":[16,114],"high":[17,115],"levels":[18,116],"therapeutic":[21,40,57,98,118,160],"protein.":[22],"A":[23],"cell":[24,112,171],"surface":[25],"protein,":[26],"not":[27,149],"normally":[28],"expressed":[29],"on":[30,151],"CHO":[31],"cells,":[32],"is":[33],"coexpressed,":[34],"as":[35],"reporter,":[37],"the":[39,52,56,70,85,92,97,121,141,152,159],"and":[42,55,125,131],"detected":[43],"using":[44],"fluorescently":[46],"labeled":[47],"antibody.":[48],"The":[49],"genes":[50],"encoding":[51],"are":[59,67,74],"linked":[60],"by":[61],"an":[62,106,137,155],"IRES,":[63],"so":[64],"that":[65],"they":[66,78],"transcribed":[68],"in":[69,140],"same":[71],"mRNA":[72],"but":[73],"translated":[75],"independently.":[76],"Since":[77],"each":[79,101],"arise":[80],"from":[81],"common":[83],"mRNA,":[84],"protein's":[87],"expression":[88,94],"level":[89,95],"accurately":[90],"predicts":[91],"relative":[93],"clone.":[102],"This":[103],"method":[104,147],"provides":[105],"effective":[107],"process":[108],"generating":[110],"recombinant":[111],"lines":[113],"proteins,":[119],"benefits":[122],"rapid":[124],"accurate":[126],"96-well":[127],"plate":[128],"clone":[129],"screening":[130],"elimination":[132],"unstable":[134],"at":[136],"earlier":[138],"development":[142,173],"process.":[143,174],"Furthermore,":[144],"because":[145],"this":[146],"does":[148],"rely":[150],"availability":[153],"antibody":[156],"specific":[157],"being":[162],"expressed,":[163],"it":[164],"can":[165],"be":[166],"easily":[167],"implemented":[168],"into":[169],"any":[170],"line":[172]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}