{"id":"https://openalex.org/W2011136039","doi":"https://doi.org/10.1101/pdb.prot093492","title":"Isolation of High-Molecular-Weight DNA from Mammalian Blood Using Proteinase K and Phenol","display_name":"Isolation of High-Molecular-Weight DNA from Mammalian Blood Using Proteinase K and Phenol","publication_year":2017,"publication_date":"2017-10-01","ids":{"openalex":"https://openalex.org/W2011136039","doi":"https://doi.org/10.1101/pdb.prot093492","mag":"2011136039","pmid":"https://pubmed.ncbi.nlm.nih.gov/28974655"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot093492","doi":"https://doi.org/10.1101/pdb.prot093492","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot093492","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060605798","display_name":"Michael R. Green","orcid":"https://orcid.org/0000-0003-3017-3298","observed_orcids":["https://orcid.org/0000-0003-3017-3298","https://orcid.org/0000-0001-6309-9472"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michael R. Green","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112152140","display_name":"Joseph Sambrook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Sambrook","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":65,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2017","issue":"10","first_page":"pdb.prot093492","last_page":"pdb.prot093492"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/genomic-dna-extraction","display_name":"genomic DNA extraction","score":0.7960000038146973},{"id":"https://openalex.org/keywords/high-molecular-weight-dna","display_name":"high molecular weight DNA","score":0.8939999938011169},{"id":"https://openalex.org/keywords/mammalian-blood","display_name":"mammalian blood","score":0.9789999723434448},{"id":"https://openalex.org/keywords/buffy-coat","display_name":"buffy coat","score":0.8389999866485596},{"id":"https://openalex.org/keywords/lymphocytes","display_name":"lymphocytes","score":0.9369999766349792},{"id":"https://openalex.org/keywords/proteinase-k","display_name":"proteinase K","score":0.8429999947547913},{"id":"https://openalex.org/keywords/phenol-extraction","display_name":"phenol extraction","score":0.9760000109672546},{"id":"https://openalex.org/keywords/dna-purification","display_name":"DNA purification","score":0.7829999923706055},{"id":"https://openalex.org/keywords/southern-blot","display_name":"Southern blot","score":0.9470000267028809}],"concepts":[{"id":"https://openalex.org/C2778285947","wikidata":"https://www.wikidata.org/wiki/Q548079","display_name":"Buffy coat","level":2,"score":0.8029142022132874},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.698161244392395},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6907483339309692},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.6380707025527954},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5583575367927551},{"id":"https://openalex.org/C2778471508","wikidata":"https://www.wikidata.org/wiki/Q425310","display_name":"Proteinase K","level":3,"score":0.5326964855194092},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.5149436593055725},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.509746253490448},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.498715877532959},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.468147337436676},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4379439353942871},{"id":"https://openalex.org/C133307689","wikidata":"https://www.wikidata.org/wiki/Q48749","display_name":"Southern blot","level":3,"score":0.4149305820465088},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36523711681365967},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3206147849559784},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.2227054238319397},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17601972818374634},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.10261747241020203},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0687154233455658},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001773","descriptor_name":"Blood Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D008967","descriptor_name":"Molecular Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019286","descriptor_name":"Endopeptidase K","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019800","descriptor_name":"Phenol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot093492","doi":"https://doi.org/10.1101/pdb.prot093492","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot093492","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:28974655","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28974655","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W126643673","https://openalex.org/W1533689894","https://openalex.org/W2037424123","https://openalex.org/W2122385641","https://openalex.org/W2417108298"],"related_works":["https://openalex.org/W4237781164","https://openalex.org/W4247120393","https://openalex.org/W2730315724","https://openalex.org/W4252476010","https://openalex.org/W2121987445","https://openalex.org/W2184537035","https://openalex.org/W4251064242","https://openalex.org/W1830573358","https://openalex.org/W2006124126","https://openalex.org/W1541555360"],"abstract_inverted_index":{"This":[0],"procedure":[1],"is":[2,43],"the":[3],"method":[4],"of":[5,9,18,30,40],"choice":[6],"for":[7,22,24],"purification":[8],"genomic":[10],"DNA":[11,19,31],"from":[12,32,37],"mammalian":[13],"blood":[14,42],"when":[15],"large":[16],"amounts":[17],"are":[20],"required,":[21],"example,":[23],"Southern":[25],"blotting.":[26],"The":[27],"usual":[28],"yield":[29],"buffy":[33],"coat":[34],"lymphocytes":[35],"isolated":[36],"20":[38],"mL":[39],"normal":[41],"\u223c250":[44],"\u00b5g.":[45]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
