{"id":"https://openalex.org/W2010980471","doi":"https://doi.org/10.1095/biolreprod47.2.277","title":"The Ability of Dehydrated Hamster and Human Sperm Nuclei to Develop into Pronuclei1","display_name":"The Ability of Dehydrated Hamster and Human Sperm Nuclei to Develop into Pronuclei1","publication_year":1992,"publication_date":"1992-08-01","ids":{"openalex":"https://openalex.org/W2010980471","doi":"https://doi.org/10.1095/biolreprod47.2.277","mag":"2010980471","pmid":"https://pubmed.ncbi.nlm.nih.gov/1391332"},"language":"en","primary_location":{"id":"doi:10.1095/biolreprod47.2.277","doi":"https://doi.org/10.1095/biolreprod47.2.277","is_oa":false,"landing_page_url":"https://doi.org/10.1095/biolreprod47.2.277","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075282803","display_name":"Haruo Katayose","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I117965899","display_name":"University of Hawai\u02bbi at M\u0101noa","ror":"https://ror.org/01wspgy28","country_code":"US","type":"education","lineage":["https://openalex.org/I117965899"]},{"id":"https://openalex.org/I1331384533","display_name":"University of Hawaii System","ror":"https://ror.org/03tzaeb71","country_code":"US","type":"education","lineage":["https://openalex.org/I1331384533"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haruo Katayose","raw_affiliation_strings":["Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822","*Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]},{"raw_affiliation_string":"*Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063614445","display_name":"Junichiro Matsuda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I117965899","display_name":"University of Hawai\u02bbi at M\u0101noa","ror":"https://ror.org/01wspgy28","country_code":"US","type":"education","lineage":["https://openalex.org/I117965899"]},{"id":"https://openalex.org/I1331384533","display_name":"University of Hawaii System","ror":"https://ror.org/03tzaeb71","country_code":"US","type":"education","lineage":["https://openalex.org/I1331384533"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Junichiro Matsuda","raw_affiliation_strings":["*Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"*Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024596573","display_name":"Ryuzo Yanagimachi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I117965899","display_name":"University of Hawai\u02bbi at M\u0101noa","ror":"https://ror.org/01wspgy28","country_code":"US","type":"education","lineage":["https://openalex.org/I117965899"]},{"id":"https://openalex.org/I1331384533","display_name":"University of Hawaii System","ror":"https://ror.org/03tzaeb71","country_code":"US","type":"education","lineage":["https://openalex.org/I1331384533"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"R. Yanagimachi","raw_affiliation_strings":["*Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"*Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5024596573"],"corresponding_institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"],"apc_list":{"value":4605,"currency":"USD","value_usd":4605},"apc_paid":null,"fwci":2.7636,"has_fulltext":false,"cited_by_count":63,"citation_normalized_percentile":{"value":0.88728194,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"47","issue":"2","first_page":"277","last_page":"284"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.4961000084877014,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.4961000084877014,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.46970000863075256,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.012799999676644802,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sperm-nucleus","display_name":"sperm nucleus","score":0.8309999704360962},{"id":"https://openalex.org/keywords/pronucleus-formation","display_name":"pronuclear formation","score":0.6629999876022339},{"id":"https://openalex.org/keywords/freeze-drying","display_name":"freeze-drying","score":0.8389999866485596},{"id":"https://openalex.org/keywords/sperm-storage","display_name":"sperm storage","score":0.6520000100135803},{"id":"https://openalex.org/keywords/hamster-sperm","display_name":"hamster sperm","score":0.5979999899864197},{"id":"https://openalex.org/keywords/human-spermatozoa","display_name":"human spermatozoa","score":0.9990000128746033},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.6150000095367432},{"id":"https://openalex.org/keywords/oocyte-microinjection","display_name":"oocyte injection","score":0.593999981880188},{"id":"https://openalex.org/keywords/chemical-dehydration","display_name":"chemical dehydration","score":0.43700000643730164},{"id":"https://openalex.org/keywords/genomic-stability","display_name":"genome stability","score":0.6389999985694885}],"concepts":[{"id":"https://openalex.org/C110758877","wikidata":"https://www.wikidata.org/wiki/Q2614103","display_name":"Pronucleus","level":5,"score":0.9915487170219421},{"id":"https://openalex.org/C2778575167","wikidata":"https://www.wikidata.org/wiki/Q6573","display_name":"Hamster","level":2,"score":0.8226377964019775},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.8070330023765564},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7542484402656555},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.5250605940818787},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.41824251413345337},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.33701643347740173},{"id":"https://openalex.org/C28748434","wikidata":"https://www.wikidata.org/wiki/Q170145","display_name":"Zygote","level":4,"score":0.3369309902191162},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.2872686982154846},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.23289760947227478},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.12858936190605164},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000432","descriptor_name":"Methanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D002725","descriptor_name":"Chloroform","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003681","descriptor_name":"Dehydration","qualifier_ui":"Q000503","qualifier_name":"physiopathology","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005612","descriptor_name":"Freeze Drying","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008647","descriptor_name":"Mesocricetus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D009865","descriptor_name":"Oocytes","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D013074","descriptor_name":"Sperm Banks","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013084","descriptor_name":"Sperm-Ovum Interactions","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1095/biolreprod47.2.277","doi":"https://doi.org/10.1095/biolreprod47.2.277","is_oa":false,"landing_page_url":"https://doi.org/10.1095/biolreprod47.2.277","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},{"id":"pmid:1391332","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1391332","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of reproduction","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5192142844","display_name":null,"funder_award_id":"HD-03402","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G536333186","display_name":"ANALYSIS OF FERTILIZATION MECHANISMS IN MAMMALS","funder_award_id":"5r01hd003402-29","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W178176341","https://openalex.org/W1037133195","https://openalex.org/W1582787799","https://openalex.org/W1859500404","https://openalex.org/W2002012992","https://openalex.org/W2026085574","https://openalex.org/W2030415964","https://openalex.org/W2055067427","https://openalex.org/W2095136374","https://openalex.org/W2125098120","https://openalex.org/W2150869497","https://openalex.org/W2156960514","https://openalex.org/W2267144837","https://openalex.org/W2506664170"],"related_works":["https://openalex.org/W2150869497","https://openalex.org/W2050436532","https://openalex.org/W2066281146","https://openalex.org/W2143379011","https://openalex.org/W2016751463","https://openalex.org/W2058401708","https://openalex.org/W1989148632","https://openalex.org/W2020054583","https://openalex.org/W2008634250","https://openalex.org/W2125098120"],"abstract_inverted_index":{"To":[0],"determine":[1],"whether":[2],"the":[3,58,67,97,114,120,134,138,142],"nuclei":[4,14,34,72,98,127,145,151],"of":[5,15,37,45,53,92,116,141,152],"mature":[6,153],"mammalian":[7],"spermatozoa":[8,19,157],"are":[9],"resistant":[10,162],"to":[11,64,148,159,163],"dehydrated":[12,102,126,143,164],"conditions,":[13],"hamster":[16,31,154],"and":[17,155],"human":[18,156],"were":[20,89],"freeze-dried":[21],"or":[22,82],"treated":[23],"with":[24,104],"various":[25],"dehydrating":[26],"agents":[27],"before":[28],"injection":[29],"into":[30,39,94,109],"oocytes.":[32],"Freeze-dried":[33],"remained":[35],"capable":[36,91],"developing":[38,93],"pronuclei":[40,121],"even":[41],"after":[42],"12":[43],"mo":[44],"storage":[46],"at":[47],"4":[48],"degrees":[49],"C.":[50],"The":[51],"level":[52,115],"DNA":[54,117],"synthetic":[55],"activity":[56],"in":[57,66,77,119,133],"sperm":[59,144],"(male)":[60],"pronucleus":[61],"was":[62,128],"comparable":[63],"that":[65,73,99,132],"egg":[68],"(female)":[69],"pronucleus.":[70],"Sperm":[71],"had":[74,100],"been":[75,101],"stored":[76],"100%":[78,80],"ethanol,":[79],"methanol,":[81],"chloroform-methanol":[83],"(2:1)":[84],"mixture":[85],"for":[86],"20":[87],"days":[88],"also":[90],"pronuclei.":[95,112,136],"Even":[96],"(\"fixed\")":[103],"Carnoy's":[105],"fluid":[106],"could":[107],"develop":[108],"morphologically":[110],"normal":[111],"However,":[113],"synthesis":[118],"derived":[122],"from":[123],"these":[124],"chemically":[125],"generally":[129],"lower":[130],"than":[131],"female":[135],"Although":[137],"genetic":[139],"integrity":[140],"is":[146],"yet":[147],"be":[149,160],"determined,":[150],"appear":[158],"fairly":[161],"conditions.":[165]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}