{"id":"https://openalex.org/W2010748293","doi":"https://doi.org/10.1016/s0168-1656(00)00378-3","title":"Purification of essentially RNA free plasmid DNA using a modified Escherichia coli host strain expressing ribonuclease A","display_name":"Purification of essentially RNA free plasmid DNA using a modified Escherichia coli host strain expressing ribonuclease A","publication_year":2001,"publication_date":"2001-02-01","ids":{"openalex":"https://openalex.org/W2010748293","doi":"https://doi.org/10.1016/s0168-1656(00)00378-3","mag":"2010748293","pmid":"https://pubmed.ncbi.nlm.nih.gov/11173096"},"language":"en","primary_location":{"id":"doi:10.1016/s0168-1656(00)00378-3","doi":"https://doi.org/10.1016/s0168-1656(00)00378-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0168-1656(00)00378-3","pdf_url":null,"source":{"id":"https://openalex.org/S182099265","display_name":"Journal of Biotechnology","issn_l":"0168-1656","issn":["0168-1656","1873-4863"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011940132","display_name":"George Dunstan Cooke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"G.D. Cooke","raw_affiliation_strings":["The Advanced Centre For Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK","Department of Biochemistry and Molecular Biology, University College London, Darwin building, Gower Street, London WC1E 6BT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Advanced Centre For Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK","institution_ids":["https://openalex.org/I45129253"]},{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University College London, Darwin building, Gower Street, London WC1E 6BT, UK","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086918152","display_name":"Rocky M. Cranenburgh","orcid":"https://orcid.org/0000-0001-5576-9157","observed_orcids":["https://orcid.org/0000-0001-5576-9157"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]},{"id":"https://openalex.org/I56007636","display_name":"Keele University","ror":"https://ror.org/00340yn33","country_code":"GB","type":"education","lineage":["https://openalex.org/I56007636"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"R.M. Cranenburgh","raw_affiliation_strings":["Cobra Therapeutics Ltd, The Science Park, University of Keele, Keele, Staffordshire ST5 5SP, UK","Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cobra Therapeutics Ltd, The Science Park, University of Keele, Keele, Staffordshire ST5 5SP, UK","institution_ids":["https://openalex.org/I56007636"]},{"raw_affiliation_string":"Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071610562","display_name":"Julian A.J. Hanak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I56007636","display_name":"Keele University","ror":"https://ror.org/00340yn33","country_code":"GB","type":"education","lineage":["https://openalex.org/I56007636"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"J.A.J. Hanak","raw_affiliation_strings":["Cobra Therapeutics Ltd, The Science Park, University of Keele, Keele, Staffordshire ST5 5SP, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cobra Therapeutics Ltd, The Science Park, University of Keele, Keele, Staffordshire ST5 5SP, UK","institution_ids":["https://openalex.org/I56007636"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108662565","display_name":"P. Dunnill","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"P. Dunnill","raw_affiliation_strings":["The Advanced Centre For Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Advanced Centre For Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK","institution_ids":["https://openalex.org/I45129253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112142473","display_name":"David R. Thatcher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I56007636","display_name":"Keele University","ror":"https://ror.org/00340yn33","country_code":"GB","type":"education","lineage":["https://openalex.org/I56007636"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"D.R. Thatcher","raw_affiliation_strings":["Cobra Therapeutics Ltd, The Science Park, University of Keele, Keele, Staffordshire ST5 5SP, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cobra Therapeutics Ltd, The Science Park, University of Keele, Keele, Staffordshire ST5 5SP, UK","institution_ids":["https://openalex.org/I56007636"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018440823","display_name":"John Maxim Ward","orcid":"https://orcid.org/0000-0002-4415-5544","observed_orcids":["https://orcid.org/0000-0002-4415-5544","https://orcid.org/0000-0001-8185-0434"]},"institutions":[{"id":"https://openalex.org/I45129253","display_name":"University College London","ror":"https://ror.org/02jx3x895","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I45129253"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"J.M. Ward","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University College London, Darwin building, Gower Street, London WC1E 6BT, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University College London, Darwin building, Gower Street, London WC1E 6BT, UK","institution_ids":["https://openalex.org/I45129253"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5018440823"],"corresponding_institution_ids":["https://openalex.org/I45129253"],"apc_list":{"value":4360,"currency":"USD","value_usd":4360},"apc_paid":null,"fwci":1.4559,"has_fulltext":false,"cited_by_count":36,"citation_normalized_percentile":{"value":0.80727487,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"85","issue":"3","first_page":"297","last_page":"304"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.4749000072479248,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.4749000072479248,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.1940000057220459,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.12269999831914902,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasmid-purification","display_name":"plasmid DNA purification","score":0.9589999914169312},{"id":"https://openalex.org/keywords/rna-removal","display_name":"RNA removal","score":0.8029999732971191},{"id":"https://openalex.org/keywords/rnase-a","display_name":"ribonuclease A","score":0.949999988079071},{"id":"https://openalex.org/keywords/bovine-pancreatic-ribonuclease-a","display_name":"bovine pancreatic ribonuclease","score":0.8410000205039978},{"id":"https://openalex.org/keywords/alkaline-lysis","display_name":"alkaline lysis","score":0.8709999918937683},{"id":"https://openalex.org/keywords/gene-therapy-vectors","display_name":"gene therapy vectors","score":0.7360000014305115},{"id":"https://openalex.org/keywords/puc18","display_name":"pUC18","score":0.7110000252723694}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.760856032371521},{"id":"https://openalex.org/C2778976237","wikidata":"https://www.wikidata.org/wiki/Q422523","display_name":"Ribonuclease","level":4,"score":0.7353755831718445},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.7171668410301208},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.7010337114334106},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.6658153533935547},{"id":"https://openalex.org/C10879258","wikidata":"https://www.wikidata.org/wiki/Q76826611","display_name":"RNase P","level":4,"score":0.6547817587852478},{"id":"https://openalex.org/C20808630","wikidata":"https://www.wikidata.org/wiki/Q4727661","display_name":"Alkaline lysis","level":5,"score":0.600767195224762},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5789229869842529},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5679384469985962},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5251431465148926},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.5055770874023438},{"id":"https://openalex.org/C109932267","wikidata":"https://www.wikidata.org/wiki/Q455079","display_name":"Plasmid preparation","level":5,"score":0.4657284617424011},{"id":"https://openalex.org/C2780704907","wikidata":"https://www.wikidata.org/wiki/Q87195451","display_name":"Pancreatic ribonuclease","level":5,"score":0.4423835873603821},{"id":"https://openalex.org/C69766542","wikidata":"https://www.wikidata.org/wiki/Q3430179","display_name":"RNase H","level":5,"score":0.4127099812030792},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3614792227745056},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32808297872543335},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29897385835647583},{"id":"https://openalex.org/C167508917","wikidata":"https://www.wikidata.org/wiki/Q578537","display_name":"DNA vaccination","level":4,"score":0.0},{"id":"https://openalex.org/C205225487","wikidata":"https://www.wikidata.org/wiki/Q287604","display_name":"PBR322","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004340","descriptor_name":"Drug Contamination","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012259","descriptor_name":"Ribonuclease, Pancreatic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012259","descriptor_name":"Ribonuclease, Pancreatic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D015316","descriptor_name":"Genetic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0168-1656(00)00378-3","doi":"https://doi.org/10.1016/s0168-1656(00)00378-3","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0168-1656(00)00378-3","pdf_url":null,"source":{"id":"https://openalex.org/S182099265","display_name":"Journal of Biotechnology","issn_l":"0168-1656","issn":["0168-1656","1873-4863"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biotechnology","raw_type":"journal-article"},{"id":"pmid:11173096","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11173096","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:10839","doi":null,"is_oa":false,"landing_page_url":"http://discovery.ucl.ac.uk/10839/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"J BIOTECHNOL , 85 (3) 297 - 304. (2001)","raw_type":"Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1502866179","https://openalex.org/W1631308596","https://openalex.org/W1793409002","https://openalex.org/W1884874540","https://openalex.org/W1963607784","https://openalex.org/W1995553769","https://openalex.org/W2014972411","https://openalex.org/W2017702389","https://openalex.org/W2019410656","https://openalex.org/W2028556087","https://openalex.org/W2028622989","https://openalex.org/W2031659110","https://openalex.org/W2042608528","https://openalex.org/W2043783598","https://openalex.org/W2048600393","https://openalex.org/W2051649700","https://openalex.org/W2064304947","https://openalex.org/W2066468289","https://openalex.org/W2082573664","https://openalex.org/W2144634347","https://openalex.org/W2148660248","https://openalex.org/W2270029128","https://openalex.org/W2284881636","https://openalex.org/W2314225431","https://openalex.org/W2403119818","https://openalex.org/W2415070274","https://openalex.org/W2469032563","https://openalex.org/W2602373563","https://openalex.org/W4250725695"],"related_works":["https://openalex.org/W3129900837","https://openalex.org/W2396166124","https://openalex.org/W3197786936","https://openalex.org/W2028556087","https://openalex.org/W2095525490","https://openalex.org/W2374301830","https://openalex.org/W2087121745","https://openalex.org/W2019217225","https://openalex.org/W1996477738","https://openalex.org/W1991493133"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
