{"id":"https://openalex.org/W2010741329","doi":"https://doi.org/10.1074/jbc.273.23.14210","title":"Human Xenomitochondrial Cybrids","display_name":"Human Xenomitochondrial Cybrids","publication_year":1998,"publication_date":"1998-06-01","ids":{"openalex":"https://openalex.org/W2010741329","doi":"https://doi.org/10.1074/jbc.273.23.14210","mag":"2010741329","pmid":"https://pubmed.ncbi.nlm.nih.gov/9603924"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.273.23.14210","doi":"https://doi.org/10.1074/jbc.273.23.14210","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.23.14210","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2010741329"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.273.23.14210","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058602584","display_name":"Antoni Barrientos","orcid":"https://orcid.org/0000-0001-9018-3231","observed_orcids":["https://orcid.org/0000-0001-9018-3231"]},"institutions":[{"id":"https://openalex.org/I145608581","display_name":"University of Miami","ror":"https://ror.org/02dgjyy92","country_code":"US","type":"education","lineage":["https://openalex.org/I145608581"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Antoni Barrientos","raw_affiliation_strings":["Department of Neurology, University of Miami, School of Medicine, Miami, Florida 33136, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurology, University of Miami, School of Medicine, Miami, Florida 33136, USA","institution_ids":["https://openalex.org/I145608581"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027948420","display_name":"Lesley Kenyon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145608581","display_name":"University of Miami","ror":"https://ror.org/02dgjyy92","country_code":"US","type":"education","lineage":["https://openalex.org/I145608581"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lesley Kenyon","raw_affiliation_strings":["Departments of Neurology, School of Medicine, Miami, Florida 33136"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Neurology, School of Medicine, Miami, Florida 33136","institution_ids":["https://openalex.org/I145608581"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060561492","display_name":"Carlos Torres Moraes","orcid":"https://orcid.org/0000-0002-8077-7092","observed_orcids":["https://orcid.org/0000-0002-8077-7092"]},"institutions":[{"id":"https://openalex.org/I145608581","display_name":"University of Miami","ror":"https://ror.org/02dgjyy92","country_code":"US","type":"education","lineage":["https://openalex.org/I145608581"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Carlos T. Moraes","raw_affiliation_strings":["Cell Biology and Anatomy, University of Miami, School of Medicine, Miami, Florida 33136","Departments of Neurology, School of Medicine, Miami, Florida 33136"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell Biology and Anatomy, University of Miami, School of Medicine, Miami, Florida 33136","institution_ids":["https://openalex.org/I145608581"]},{"raw_affiliation_string":"Departments of Neurology, School of Medicine, Miami, Florida 33136","institution_ids":["https://openalex.org/I145608581"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5060561492"],"corresponding_institution_ids":["https://openalex.org/I145608581"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.1316,"has_fulltext":false,"cited_by_count":188,"citation_normalized_percentile":{"value":0.87713492,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"273","issue":"23","first_page":"14210","last_page":"14217"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11027","display_name":"Metabolism and Genetic Disorders","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mitochondrial-complex-i-deficiency","display_name":"mitochondrial complex I deficiency","score":0.7770000100135803},{"id":"https://openalex.org/keywords/oxidative-phosphorylation","display_name":"oxidative phosphorylation","score":0.8939999938011169},{"id":"https://openalex.org/keywords/mitochondrial-dna-transfer","display_name":"mitochondrial DNA transfer","score":0.5440000295639038},{"id":"https://openalex.org/keywords/chimpanzees","display_name":"chimpanzees","score":0.5550000071525574},{"id":"https://openalex.org/keywords/gorillas","display_name":"gorillas","score":0.8040000200271606},{"id":"https://openalex.org/keywords/rotenone-sensitivity","display_name":"rotenone sensitivity","score":0.6589999794960022},{"id":"https://openalex.org/keywords/mitochondrial-respiration","display_name":"mitochondrial respiration","score":0.3779999911785126}],"concepts":[{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.3642374575138092}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006822","descriptor_name":"Hybrid Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010085","descriptor_name":"Oxidative Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010101","descriptor_name":"Oxygen Consumption","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D012402","descriptor_name":"Rotenone","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015186","descriptor_name":"Hominidae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016660","descriptor_name":"NAD(P)H Dehydrogenase (Quinone)","qualifier_ui":"Q000172","qualifier_name":"deficiency","is_major_topic":false},{"descriptor_ui":"D016660","descriptor_name":"NAD(P)H Dehydrogenase (Quinone)","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019069","descriptor_name":"Cell Respiration","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D019069","descriptor_name":"Cell Respiration","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D019143","descriptor_name":"Evolution, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.273.23.14210","doi":"https://doi.org/10.1074/jbc.273.23.14210","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.23.14210","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:9603924","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9603924","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.273.23.14210","doi":"https://doi.org/10.1074/jbc.273.23.14210","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.273.23.14210","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5999000072479248,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4444068357","display_name":"EXPLORING NUCLEAR-MITOCHONDRIAL DNA INTERACTIONS","funder_award_id":"R01GM055766","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W126252753","https://openalex.org/W314960756","https://openalex.org/W1026284996","https://openalex.org/W1680438645","https://openalex.org/W1899924400","https://openalex.org/W1967590676","https://openalex.org/W1970345057","https://openalex.org/W1994857842","https://openalex.org/W1998730765","https://openalex.org/W2001700140","https://openalex.org/W2001763525","https://openalex.org/W2008131726","https://openalex.org/W2010986573","https://openalex.org/W2012003686","https://openalex.org/W2012947090","https://openalex.org/W2017347785","https://openalex.org/W2019042812","https://openalex.org/W2024034891","https://openalex.org/W2039280872","https://openalex.org/W2041850796","https://openalex.org/W2041867957","https://openalex.org/W2042734316","https://openalex.org/W2050057361","https://openalex.org/W2076971346","https://openalex.org/W2077311097","https://openalex.org/W2091390708","https://openalex.org/W2091696298","https://openalex.org/W2103804977","https://openalex.org/W2104269538","https://openalex.org/W2109291482","https://openalex.org/W2112602336","https://openalex.org/W2128635872","https://openalex.org/W2129398678","https://openalex.org/W2137999969","https://openalex.org/W2993552196","https://openalex.org/W3145894777","https://openalex.org/W4211198726","https://openalex.org/W4254119740","https://openalex.org/W6892424456"],"related_works":["https://openalex.org/W1967590676","https://openalex.org/W4220855487","https://openalex.org/W2065846878","https://openalex.org/W1969400804","https://openalex.org/W3010276449","https://openalex.org/W1970785287","https://openalex.org/W2091952491","https://openalex.org/W2171715833","https://openalex.org/W2120112437","https://openalex.org/W1983505595"],"abstract_inverted_index":{"The":[0],"subunits":[1,164],"forming":[2],"the":[3,102,112,115,200],"mitochondrial":[4,14,205],"oxidative":[5,54,210],"phosphorylation":[6,55,211],"system":[7],"are":[8],"coded":[9],"by":[10,108],"both":[11],"nuclear":[12,203],"and":[13,34,42,65,111,131,170,198,204],"genes.":[15],"Recently,":[16],"we":[17,48],"attempted":[18],"to":[19],"introduce":[20],"mtDNA":[21,31],"from":[22,70],"non-human":[23,74],"apes":[24],"into":[25],"a":[26,50,79,143,153],"human":[27,72],"cell":[28,129],"line":[29],"lacking":[30],"(rho":[32],"degrees),":[33],"succeeded":[35],"in":[36,57,85,106,134,146,182,196,208],"producing":[37],"human-common":[38],"chimpanzee,":[39,41],"human-pigmy":[40],"human-gorilla":[43],"xenomitochondrial":[44],"cybrids":[45],"(HXC).":[46],"Here,":[47],"present":[49],"comprehensive":[51],"characterization":[52],"of":[53,90,127,156,162,165,192,202],"function":[56],"these":[58],"cells.":[59,76,184],"Mitochondrial":[60],"complexes":[61],"II,":[62],"III,":[63],"IV,":[64],"V":[66],"had":[67],"activities":[68],"indistinguishable":[69],"parental":[71],"or":[73,95],"primate":[75],"In":[77],"contrast,":[78],"complex":[80,91,166,193],"I":[81,92,167,194],"deficiency":[82,195],"was":[83,104,117],"observed":[84],"all":[86],"HXC.":[87],"Kinetic":[88],"studies":[89,126],"using":[93],"decylubiquinone":[94],"NADH":[96,116],"as":[97,189],"limiting":[98],"substrates":[99],"showed":[100,137,173],"that":[101,138,174],"Vmax":[103],"decreased":[105],"HXC":[107,157,183,188],"approximately":[109],"40%,":[110],"Km":[113],"for":[114],"significantly":[118,180],"increased":[119],"(3-fold,":[120],"p":[121],"<":[122],"0.001).":[123],"Rotenone":[124],"inhibition":[125,155],"intact":[128],"respiration":[130,158],"pyruvate-malate":[132],"oxidation":[133],"permeabilized":[135],"cells":[136,148],"3":[139],"nM":[140],"rotenone":[141],"produced":[142,152],"mild":[144],"effect":[145],"control":[147],"(0-10%":[149],"inhibition)":[150],"but":[151],"marked":[154],"(50-75%).":[159],"Immunoblotting":[160],"analyses":[161],"three":[163],"(ND1,":[168],"75":[169],"49":[171],"kDa)":[172],"their":[175],"relative":[176],"amounts":[177],"were":[178],"not":[179],"altered":[181],"These":[185],"results":[186],"establish":[187],"cellular":[190],"models":[191],"humans":[197],"underscore":[199],"importance":[201],"genomes":[206],"co-evolution":[207],"optimizing":[209],"function.":[212]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}