{"id":"https://openalex.org/W2010575752","doi":"https://doi.org/10.1002/jcb.23391","title":"A Simple and economical route to generate functional hepatocyte\u2010like cells from hESCs and their application in evaluating alcohol induced liver damage","display_name":"A Simple and economical route to generate functional hepatocyte\u2010like cells from hESCs and their application in evaluating alcohol induced liver damage","publication_year":2011,"publication_date":"2011-09-28","ids":{"openalex":"https://openalex.org/W2010575752","doi":"https://doi.org/10.1002/jcb.23391","mag":"2010575752","pmid":"https://pubmed.ncbi.nlm.nih.gov/21956183"},"language":"en","primary_location":{"id":"doi:10.1002/jcb.23391","doi":"https://doi.org/10.1002/jcb.23391","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jcb.23391","pdf_url":null,"source":{"id":"https://openalex.org/S53807158","display_name":"Journal of Cellular Biochemistry","issn_l":"0730-2312","issn":["0730-2312","1097-4644"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cellular Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007233304","display_name":"Rajarshi Pal","orcid":"https://orcid.org/0000-0001-6334-4562","observed_orcids":["https://orcid.org/0000-0001-6334-4562"]},"institutions":[{"id":"https://openalex.org/I164861460","display_name":"Manipal Academy of Higher Education","ror":"https://ror.org/02xzytt36","country_code":"IN","type":"education","lineage":["https://openalex.org/I164861460"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rajarshi Pal","raw_affiliation_strings":["Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, Domlur Layout, Bangalore 560071, India","Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, Domlur Layout, Bangalore 560071, India","institution_ids":["https://openalex.org/I164861460"]},{"raw_affiliation_string":"Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India","institution_ids":["https://openalex.org/I164861460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039735514","display_name":"Murali Krishna Mamidi","orcid":"https://orcid.org/0009-0009-5021-4342","observed_orcids":["https://orcid.org/0009-0009-5021-4342"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Murali Krishna Mamidi","raw_affiliation_strings":["Stempeutics Research Malaysia Sdn. Bhd, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stempeutics Research Malaysia Sdn. Bhd, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102894860","display_name":"Anjan Kumar Das","orcid":"https://orcid.org/0000-0001-6318-4669","observed_orcids":["https://orcid.org/0000-0001-6318-4669"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anjan Kumar Das","raw_affiliation_strings":["Stempeutics Research Malaysia Sdn. Bhd, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stempeutics Research Malaysia Sdn. Bhd, Enterprise 4, Technology Park Malaysia, Bukit Jalil, 57000 Kuala Lumpur, Malaysia","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087556351","display_name":"Pawan Kumar Gupta","orcid":"https://orcid.org/0000-0002-8392-270X","observed_orcids":["https://orcid.org/0000-0002-8392-270X"]},"institutions":[{"id":"https://openalex.org/I164861460","display_name":"Manipal Academy of Higher Education","ror":"https://ror.org/02xzytt36","country_code":"IN","type":"education","lineage":["https://openalex.org/I164861460"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Pawan Kumar Gupta","raw_affiliation_strings":["Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore 560071, India","Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore 560071, India","institution_ids":["https://openalex.org/I164861460"]},{"raw_affiliation_string":"Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India","institution_ids":["https://openalex.org/I164861460"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084809927","display_name":"Ramesh Ramchandra Bhonde","orcid":"https://orcid.org/0000-0001-9955-2056","observed_orcids":["https://orcid.org/0000-0001-9955-2056"]},"institutions":[{"id":"https://openalex.org/I164861460","display_name":"Manipal Academy of Higher Education","ror":"https://ror.org/02xzytt36","country_code":"IN","type":"education","lineage":["https://openalex.org/I164861460"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Ramesh Bhonde","raw_affiliation_strings":["Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore 560071, India","Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore 560071, India","institution_ids":["https://openalex.org/I164861460"]},{"raw_affiliation_string":"Manipal Institute of Regenerative Medicine, Manipal University Branch Campus, # 10 Service Road, Domlur Layout, Bangalore, 560071, India","institution_ids":["https://openalex.org/I164861460"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5084809927"],"corresponding_institution_ids":["https://openalex.org/I164861460"],"apc_list":{"value":5110,"currency":"USD","value_usd":5110},"apc_paid":null,"fwci":1.5276,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.81286914,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"113","issue":"1","first_page":"19","last_page":"30"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatocyte-like-cells","display_name":"hepatocyte-like cells","score":0.8610000014305115},{"id":"https://openalex.org/keywords/human-embryonic-stem-cells","display_name":"human embryonic stem cells","score":0.9879999756813049},{"id":"https://openalex.org/keywords/hepatocyte-differentiation","display_name":"hepatocyte differentiation","score":0.7310000061988831},{"id":"https://openalex.org/keywords/conditioned-medium","display_name":"conditioned medium","score":0.6129999756813049},{"id":"https://openalex.org/keywords/hepg2-cells","display_name":"HepG2 cells","score":0.875},{"id":"https://openalex.org/keywords/definitive-endoderm","display_name":"definitive endoderm","score":0.7319999933242798},{"id":"https://openalex.org/keywords/liver-development","display_name":"liver development","score":0.531000018119812},{"id":"https://openalex.org/keywords/prenatal-alcohol-exposure","display_name":"prenatal alcohol exposure","score":0.8370000123977661},{"id":"https://openalex.org/keywords/ethanol-induced-liver-damage","display_name":"ethanol-induced liver damage","score":0.7749999761581421},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8169999718666077},{"id":"https://openalex.org/keywords/hepatogenesis","display_name":"hepatogenesis","score":0.3930000066757202},{"id":"https://openalex.org/keywords/cyp3a4","display_name":"CYP3A4","score":0.7519999742507935},{"id":"https://openalex.org/keywords/cyp7a1","display_name":"CYP7A1","score":0.7440000176429749},{"id":"https://openalex.org/keywords/hnf3beta","display_name":"HNF-3beta","score":0.6669999957084656}],"concepts":[{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6813798546791077},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.652347207069397},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.5834622383117676},{"id":"https://openalex.org/C2777164004","wikidata":"https://www.wikidata.org/wiki/Q331858","display_name":"Endoderm","level":4,"score":0.5678809285163879},{"id":"https://openalex.org/C2776200302","wikidata":"https://www.wikidata.org/wiki/Q827450","display_name":"Hepatocyte","level":3,"score":0.5557512044906616},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5221024751663208},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.43784767389297485},{"id":"https://openalex.org/C2777043721","wikidata":"https://www.wikidata.org/wiki/Q369887","display_name":"Yolk sac","level":3,"score":0.4255136251449585},{"id":"https://openalex.org/C195521686","wikidata":"https://www.wikidata.org/wiki/Q17152676","display_name":"Liver cytology","level":3,"score":0.41764116287231445},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.40934139490127563},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.39499881863594055},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.2431243658065796},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.1872740089893341},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.183648943901062},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1664654016494751},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1599847674369812},{"id":"https://openalex.org/C2908801736","wikidata":"https://www.wikidata.org/wiki/Q9368","display_name":"Liver metabolism","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008108","descriptor_name":"Liver Diseases, Alcoholic","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D008108","descriptor_name":"Liver Diseases, Alcoholic","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D008108","descriptor_name":"Liver Diseases, Alcoholic","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017077","descriptor_name":"Culture Media, Conditioned","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D038081","descriptor_name":"Organogenesis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D038081","descriptor_name":"Organogenesis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D038081","descriptor_name":"Organogenesis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D053595","descriptor_name":"Embryonic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D053595","descriptor_name":"Embryonic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D053595","descriptor_name":"Embryonic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D056945","descriptor_name":"Hep G2 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056945","descriptor_name":"Hep G2 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056945","descriptor_name":"Hep G2 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/jcb.23391","doi":"https://doi.org/10.1002/jcb.23391","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jcb.23391","pdf_url":null,"source":{"id":"https://openalex.org/S53807158","display_name":"Journal of Cellular Biochemistry","issn_l":"0730-2312","issn":["0730-2312","1097-4644"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cellular Biochemistry","raw_type":"journal-article"},{"id":"pmid:21956183","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21956183","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cellular biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.98580002784729,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","score":0.49000000953674316,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W25071394","https://openalex.org/W1967676765","https://openalex.org/W1970023965","https://openalex.org/W1977668459","https://openalex.org/W1983060510","https://openalex.org/W1988205098","https://openalex.org/W1998893666","https://openalex.org/W1999914688","https://openalex.org/W2001630726","https://openalex.org/W2027254129","https://openalex.org/W2043457834","https://openalex.org/W2052497886","https://openalex.org/W2053355345","https://openalex.org/W2066872527","https://openalex.org/W2073477529","https://openalex.org/W2077473810","https://openalex.org/W2077547257","https://openalex.org/W2092603433","https://openalex.org/W2093236558","https://openalex.org/W2094689158","https://openalex.org/W2110335719","https://openalex.org/W2121784259","https://openalex.org/W2124914558","https://openalex.org/W2126683640","https://openalex.org/W2135670457","https://openalex.org/W2137175341","https://openalex.org/W2138887469","https://openalex.org/W2142769704","https://openalex.org/W2152530502","https://openalex.org/W2155942576","https://openalex.org/W2169119281","https://openalex.org/W2416064936"],"related_works":["https://openalex.org/W1982329297","https://openalex.org/W2104244471","https://openalex.org/W2404859484","https://openalex.org/W2053963861","https://openalex.org/W2040909135","https://openalex.org/W2039177759","https://openalex.org/W2258332741","https://openalex.org/W2429566226","https://openalex.org/W2115589900","https://openalex.org/W2254678890"],"abstract_inverted_index":{"The":[0],"in":[1,76,97,145,182,191,228,236],"vitro":[2,192],"derived":[3],"hepatocytes":[4,121,194],"from":[5,46,58],"human":[6,27,47],"embryonic":[7,155],"stem":[8],"cells":[9,57,167,185,219],"(hESC)":[10],"is":[11,176],"a":[12,38,150],"promising":[13],"tool":[14],"to":[15,53,178,195],"acquire":[16],"improved":[17],"knowledge":[18],"of":[19,81,87,102,153,217,231,247,256],"the":[20,70,79,85,100,103,136,164,183,206,229,244,253],"cellular":[21],"and":[22,32,65,78,96,163,186,198,208,214,233],"molecular":[23],"events":[24],"underlying":[25],"early":[26],"liver":[28,147,170,257],"development":[29,148],"under":[30],"physiological":[31],"pathological":[33],"conditions.":[34],"Here":[35],"we":[36,189],"report":[37],"simple":[39],"two-step":[40],"protocol":[41],"employing":[42],"conditioned":[43],"medium":[44],"(CM)":[45],"hepatocellular":[48],"carcinoma":[49],"cell":[50],"line,":[51],"HepG2":[52],"generate":[54],"functional":[55,120],"hepatocyte-like":[56],"hESC.":[59],"Immunocytochemistry,":[60],"flow":[61],"cytometry,":[62],"quantitative":[63],"RT-PCR,":[64],"biochemical":[66],"analyses":[67],"revealed":[68],"that":[69,135,200],"endodermal":[71,156],"progenitors":[72,107],"appeared":[73],"as":[74],"pockets":[75],"culture,":[77],"cascade":[80],"genes":[82,232],"associated":[83],"with":[84,99],"formation":[86],"definitive":[88],"endoderm":[89],"(HNF-3\u03b2,":[90],"SOX-17,":[91],"DLX-5,":[92],"CXCR4)":[93],"was":[94,224],"consistent":[95],"concurrence":[98],"up-regulation":[101],"markers":[104,157],"for":[105],"hepatic":[106,166],"[alpha-feto":[108],"protein":[109],"(AFP),":[110],"HNF-4\u03b1,":[111],"CK-19,":[112],"albumin,":[113],"alpha-1-antitrypsin":[114],"(AAT)],":[115],"followed":[116],"by":[117,226,250],"maturation":[118],"into":[119,220,243],"[tyrosine":[122],"transferase":[123],"(TAT),":[124],"tryptophan-2,":[125],"3-dioxygenase":[126],"(TDO),":[127],"glucose":[128],"6-phosphate":[129],"(G6P),":[130],"CYP3A4,":[131],"CYP7A1].":[132],"We":[133],"witnessed":[134],"gene":[137],"expression":[138,230],"profile":[139],"during":[140],"this":[141],"differentiation":[142,216],"process":[143,255],"recapitulated":[144],"vivo":[146],"demonstrating":[149],"gradual":[151],"down-regulation":[152],"extra":[154],"(SOX-7,":[158],"HNF-1\u03b2,":[159],"SNAIL-1,":[160],"LAMININ-1,":[161],"CDX2),":[162],"generated":[165,193],"performed":[168],"multiple":[169],"functions.":[171],"Since":[172],"prenatal":[173],"alcohol":[174,251],"exposure":[175],"known":[177],"provoke":[179],"irreversible":[180],"abnormalities":[181],"fetal":[184],"developing":[187],"tissues,":[188],"exposed":[190],"ethanol":[196],"(EtOH)":[197],"found":[199],"EtOH":[201],"treatment":[202],"not":[203],"only":[204],"impairs":[205],"survival":[207],"proliferation,":[209],"but":[210],"also":[211],"induces":[212],"apoptosis":[213],"perturbs":[215],"progenitor":[218],"hepatocytes.":[221],"This":[222],"disruption":[223],"accompanied":[225],"alterations":[227],"proteins":[234],"involved":[235],"hepatogenesis.":[237],"Our":[238],"results":[239],"provide":[240],"new":[241],"insights":[242],"wider":[245],"range":[246],"destruction":[248],"caused":[249],"on":[252],"dynamic":[254],"organogenesis.":[258]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}