{"id":"https://openalex.org/W2010506842","doi":"https://doi.org/10.1016/s1044-0305(94)00097-j","title":"Detection of noncovalent FKBP-FK506 and FKBP-Rapamycin complexes by capillary electrophoresis-mass spectrometry and capillary electrophoresis-tandem mass spectrometry","display_name":"Detection of noncovalent FKBP-FK506 and FKBP-Rapamycin complexes by capillary electrophoresis-mass spectrometry and capillary electrophoresis-tandem mass spectrometry","publication_year":1995,"publication_date":"1995-02-01","ids":{"openalex":"https://openalex.org/W2010506842","doi":"https://doi.org/10.1016/s1044-0305(94)00097-j","mag":"2010506842","pmid":"https://pubmed.ncbi.nlm.nih.gov/24222070"},"language":"en","primary_location":{"id":"doi:10.1016/s1044-0305(94)00097-j","doi":"https://doi.org/10.1016/s1044-0305(94)00097-j","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s1044-0305(94)00097-j","pdf_url":null,"source":{"id":"https://openalex.org/S60743949","display_name":"Journal of the American Society for Mass Spectrometry","issn_l":"1044-0305","issn":["1044-0305","1879-1123"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060960444","display_name":"Yin-Liang Hsieh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yin-Liang Hsieh","raw_affiliation_strings":["Analytical Toxicology, Cornell University, Ithaca, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Analytical Toxicology, Cornell University, Ithaca, New York, USA","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047277605","display_name":"Jianyi Cai","orcid":"https://orcid.org/0000-0003-0138-1733","observed_orcids":["https://orcid.org/0000-0003-0138-1733"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianyi Cai","raw_affiliation_strings":["Analytical Toxicology, Cornell University, Ithaca, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Analytical Toxicology, Cornell University, Ithaca, New York, USA","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023071838","display_name":"Yu-Tsyr Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yu-Tsyr Li","raw_affiliation_strings":["Analytical Toxicology, Cornell University, Ithaca, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Analytical Toxicology, Cornell University, Ithaca, New York, USA","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054043854","display_name":"Jack D. Henion","orcid":"https://orcid.org/0000-0002-9271-6975","observed_orcids":["https://orcid.org/0000-0002-9271-6975"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jack D. Henion","raw_affiliation_strings":["Analytical Toxicology, Cornell University, Ithaca, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Analytical Toxicology, Cornell University, Ithaca, New York, USA","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5035275755","display_name":"Bruce Ganem","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bruce Ganem","raw_affiliation_strings":["Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York, USA","institution_ids":["https://openalex.org/I205783295"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I205783295"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":3.1009,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.92055064,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"6","issue":"2","first_page":"85","last_page":"90"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9531000256538391,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9531000256538391,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.02669999934732914,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fkbp","display_name":"FKBP","score":0.9779999852180481},{"id":"https://openalex.org/keywords/fk506","display_name":"FK506","score":0.984000027179718},{"id":"https://openalex.org/keywords/rapamycin","display_name":"rapamycin","score":0.8970000147819519},{"id":"https://openalex.org/keywords/immunophilins","display_name":"immunophilins","score":0.5929999947547913},{"id":"https://openalex.org/keywords/receptor-ligand-complex","display_name":"receptor-ligand complex","score":0.8410000205039978},{"id":"https://openalex.org/keywords/noncovalent-interactions","display_name":"non-covalent interactions","score":0.8899999856948853},{"id":"https://openalex.org/keywords/capillary-electrophoresis-mass-spectrometry","display_name":"capillary electrophoresis-mass spectrometry","score":0.902999997138977},{"id":"https://openalex.org/keywords/multiple-reaction-monitoring","display_name":"multiple reaction monitoring","score":0.8309999704360962},{"id":"https://openalex.org/keywords/selected-ion-monitoring","display_name":"selected ion monitoring","score":0.8640000224113464},{"id":"https://openalex.org/keywords/immunosuppressive-drugs","display_name":"immunosuppressants","score":0.8159999847412109},{"id":"https://openalex.org/keywords/complex-stoichiometry","display_name":"complex stoichiometry","score":0.6110000014305115}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9213177561759949},{"id":"https://openalex.org/C133956753","wikidata":"https://www.wikidata.org/wiki/Q583864","display_name":"FKBP","level":2,"score":0.8095232844352722},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.756046712398529},{"id":"https://openalex.org/C2900643","wikidata":"https://www.wikidata.org/wiki/Q747468","display_name":"Capillary electrophoresis","level":2,"score":0.7312516570091248},{"id":"https://openalex.org/C88142848","wikidata":"https://www.wikidata.org/wiki/Q874035","display_name":"Capillary electrophoresis\u2013mass spectrometry","level":4,"score":0.6157403588294983},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6032470464706421},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.5974835753440857},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.4266428053379059},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.19574660062789917},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.07764238119125366}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1016/s1044-0305(94)00097-j","doi":"https://doi.org/10.1016/s1044-0305(94)00097-j","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s1044-0305(94)00097-j","pdf_url":null,"source":{"id":"https://openalex.org/S60743949","display_name":"Journal of the American Society for Mass Spectrometry","issn_l":"1044-0305","issn":["1044-0305","1879-1123"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"journal-article"},{"id":"pmid:24222070","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24222070","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society for Mass Spectrometry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W577065181","https://openalex.org/W609901773","https://openalex.org/W1969242164","https://openalex.org/W1971177037","https://openalex.org/W1978879981","https://openalex.org/W2003022329","https://openalex.org/W2003695026","https://openalex.org/W2004645109","https://openalex.org/W2014418564","https://openalex.org/W2015191353","https://openalex.org/W2017383844","https://openalex.org/W2020976582","https://openalex.org/W2022042248","https://openalex.org/W2024358788","https://openalex.org/W2024842574","https://openalex.org/W2024995712","https://openalex.org/W2025914365","https://openalex.org/W2026398915","https://openalex.org/W2036088145","https://openalex.org/W2039018014","https://openalex.org/W2039552227","https://openalex.org/W2044546079","https://openalex.org/W2048373800","https://openalex.org/W2050633231","https://openalex.org/W2056959896","https://openalex.org/W2059549595","https://openalex.org/W2059953588","https://openalex.org/W2067247087","https://openalex.org/W2084977836","https://openalex.org/W2086354229","https://openalex.org/W2087801626","https://openalex.org/W2089177209","https://openalex.org/W2093804891","https://openalex.org/W2115708822","https://openalex.org/W2329360175","https://openalex.org/W2402699220","https://openalex.org/W4255940022","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2335164643","https://openalex.org/W2026594194","https://openalex.org/W1978397978","https://openalex.org/W593959866","https://openalex.org/W379035195","https://openalex.org/W1971556344","https://openalex.org/W2023061017","https://openalex.org/W4410490262","https://openalex.org/W4206751728","https://openalex.org/W2089579242"],"abstract_inverted_index":{"The":[0],"well":[1,122],"known":[2],"biospecific":[3],"noncovalent":[4,187],"receptor-ligand":[5],"association":[6],"complexes":[7,99,111,161],"between":[8,112,156],"the":[9,13,76,97,109,145,157,167],"immunophilin":[10],"FKBP":[11,113,124],"and":[12,17,34,93,96,114,125,140,159,177],"immunosuppressive":[14],"drugs":[15],"FK506":[16,69,115,118,139,160],"Rapamycin":[18],"(RM)":[19],"were":[20,53,73,88,100],"investigated":[21],"by":[22,35,90,102],"on-line":[23],"capillary":[24],"electrophoresis-mass":[25],"spectrometry":[26,40],"(CE-MS)":[27],"under":[28,42,75,193],"selected":[29,43],"ion":[30,153],"monitoring":[31,45],"(SIM)":[32],"conditions":[33],"CE-MS":[36,92,95,176],"with":[37,104],"tandem":[38],"mass":[39,105],"(CE-MS/MS)":[41],"reaction":[44],"(SRM)":[46],"conditions.":[47,79,195],"Solutions":[48],"of":[49,68,108,138,147,152,189],"hFKBP":[50],"(33.3":[51],"\u00b5M)":[52],"dissolved":[54],"in":[55,144,166],"50":[56],"mM":[57],"ammonium":[58],"acetate":[59],"at":[60],"pH":[61,84],"7.5.":[62],"Samples":[63],"that":[64,175],"contained":[65],"100":[66],"\u00b5M":[67],"or":[70],"RM":[71,126,129,141,158],"also":[72],"prepared":[74],"same":[77],"solution":[78],"By":[80],"using":[81],"these":[82],"aqueous":[83],"neutral":[85],"conditions,":[86],"samples":[87],"analyzed":[89,143],"SIM":[91],"SRM":[94],"target":[98],"separated":[101],"CE":[103],"spectrometer":[106],"detection":[107],"individual":[110],"[hFKBP":[116,127],"+":[117,119,128,130],"7HJ(7+)":[120],"as":[121,123,164],"7HJ(7+).":[131],"In":[132],"an":[133],"experiment":[134],"where":[135],"a":[136,149],"mixture":[137],"was":[142,162],"presence":[146],"FKBP,":[148],"nine-to-one":[150],"ratio":[151],"current":[154],"abundances":[155],"observed":[163],"reported":[165],"literature":[168],"from":[169],"other":[170],"studies.":[171],"These":[172],"results":[173],"suggest":[174],"CE-MS/MS":[178],"may":[179],"be":[180],"yet":[181],"another":[182],"analytical":[183],"method":[184],"for":[185],"studying":[186],"interactions":[188],"biologically":[190],"important":[191],"macromolecules":[192],"physiological":[194]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":2}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}