{"id":"https://openalex.org/W2010426465","doi":"https://doi.org/10.1016/0022-4731(89)90307-5","title":"A convenient, unified scheme for the differential extraction of conjugated and unconjugated serum C19 steroids on Sep-Pak C18-cartridges","display_name":"A convenient, unified scheme for the differential extraction of conjugated and unconjugated serum C19 steroids on Sep-Pak C18-cartridges","publication_year":1989,"publication_date":"1989-08-01","ids":{"openalex":"https://openalex.org/W2010426465","doi":"https://doi.org/10.1016/0022-4731(89)90307-5","mag":"2010426465","pmid":"https://pubmed.ncbi.nlm.nih.gov/2770301"},"language":"en","primary_location":{"id":"doi:10.1016/0022-4731(89)90307-5","doi":"https://doi.org/10.1016/0022-4731(89)90307-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0022-4731(89)90307-5","pdf_url":null,"source":{"id":"https://openalex.org/S104308233","display_name":"Journal of Steroid Biochemistry","issn_l":"0022-4731","issn":["0022-4731","1878-2353"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320146","host_organization_name":"Pergamon Press","host_organization_lineage":["https://openalex.org/P4310320146"],"host_organization_lineage_names":["Pergamon Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Steroid Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000028306","display_name":"Donna W. Payne","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]},{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Donna W. Payne","raw_affiliation_strings":["Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore 21201","Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore, Maryland 21201, U.S.A","Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore 21201","institution_ids":["https://openalex.org/I126744593"]},{"raw_affiliation_string":"Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore, Maryland 21201, U.S.A","institution_ids":["https://openalex.org/I126744593"]},{"raw_affiliation_string":"Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048525964","display_name":"Walter David Holtzclaw","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145311948","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95","country_code":"US","type":"education","lineage":["https://openalex.org/I145311948"]},{"id":"https://openalex.org/I2799853436","display_name":"Johns Hopkins Medicine","ror":"https://ror.org/037zgn354","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799853436"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W.David Holtzclaw","raw_affiliation_strings":["Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A","institution_ids":["https://openalex.org/I145311948","https://openalex.org/I2799853436"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086044543","display_name":"Eli Y. Adashi","orcid":"https://orcid.org/0000-0001-7730-1192","observed_orcids":["https://orcid.org/0000-0001-7730-1192"]},"institutions":[{"id":"https://openalex.org/I126744593","display_name":"University of Maryland, Baltimore","ror":"https://ror.org/04rq5mt64","country_code":"US","type":"education","lineage":["https://openalex.org/I126744593"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eli Y. Adashi","raw_affiliation_strings":["Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore, Maryland 21201, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore, Maryland 21201, U.S.A","institution_ids":["https://openalex.org/I126744593"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5000028306"],"corresponding_institution_ids":["https://openalex.org/I126744593","https://openalex.org/I145311948","https://openalex.org/I2799853436"],"apc_list":null,"apc_paid":null,"fwci":0.5811,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.66563786,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"33","issue":"2","first_page":"289","last_page":"295"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11146","display_name":"Hormonal and reproductive studies","score":0.5708000063896179,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11146","display_name":"Hormonal and reproductive studies","score":0.5708000063896179,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10180","display_name":"Analytical chemistry methods development","score":0.16189999878406525,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.10620000213384628,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/differential-extraction","display_name":"differential extraction","score":0.7820000052452087},{"id":"https://openalex.org/keywords/serum-androgens","display_name":"serum androgens","score":0.7229999899864197},{"id":"https://openalex.org/keywords/steroid-glucuronides","display_name":"steroid glucuronides","score":0.7689999938011169},{"id":"https://openalex.org/keywords/steroid-sulfates","display_name":"steroid sulfates","score":0.9940000176429749},{"id":"https://openalex.org/keywords/sep-pak-c18","display_name":"Sep-Pak C18","score":0.7490000128746033},{"id":"https://openalex.org/keywords/solid-phase-extraction","display_name":"solid-phase extraction","score":0.6809999942779541},{"id":"https://openalex.org/keywords/enzymatic-hydrolysis","display_name":"enzymatic hydrolysis","score":0.8820000290870667},{"id":"https://openalex.org/keywords/solvolysis","display_name":"solvolysis","score":0.7789999842643738},{"id":"https://openalex.org/keywords/testosterone","display_name":"testosterone","score":0.7889999747276306},{"id":"https://openalex.org/keywords/17beta-hydroxy-steroids","display_name":"17\u03b2-hydroxy steroids","score":0.722000002861023},{"id":"https://openalex.org/keywords/human-serum","display_name":"human serum","score":0.6079999804496765}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8603593111038208},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.8144493103027344},{"id":"https://openalex.org/C2780902042","wikidata":"https://www.wikidata.org/wiki/Q177911","display_name":"Steroid","level":3,"score":0.7086936235427856},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.6090044975280762},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.5670548677444458},{"id":"https://openalex.org/C2779607525","wikidata":"https://www.wikidata.org/wiki/Q14982","display_name":"Methanol","level":2,"score":0.5343450903892517},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.5062834024429321},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4561549723148346},{"id":"https://openalex.org/C53270655","wikidata":"https://www.wikidata.org/wiki/Q5381536","display_name":"Enzymatic hydrolysis","level":3,"score":0.4411436915397644},{"id":"https://openalex.org/C149629883","wikidata":"https://www.wikidata.org/wiki/Q660926","display_name":"Fraction (chemistry)","level":2,"score":0.4186535179615021},{"id":"https://openalex.org/C2780365088","wikidata":"https://www.wikidata.org/wiki/Q48318","display_name":"Urea","level":2,"score":0.413421630859375},{"id":"https://openalex.org/C2779293432","wikidata":"https://www.wikidata.org/wiki/Q2628534","display_name":"Solvolysis","level":3,"score":0.41295409202575684},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25706052780151367},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.19690173864364624},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1689114272594452}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013256","descriptor_name":"Steroids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013256","descriptor_name":"Steroids","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D013256","descriptor_name":"Steroids","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/0022-4731(89)90307-5","doi":"https://doi.org/10.1016/0022-4731(89)90307-5","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0022-4731(89)90307-5","pdf_url":null,"source":{"id":"https://openalex.org/S104308233","display_name":"Journal of Steroid Biochemistry","issn_l":"0022-4731","issn":["0022-4731","1878-2353"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320146","host_organization_name":"Pergamon Press","host_organization_lineage":["https://openalex.org/P4310320146"],"host_organization_lineage_names":["Pergamon Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Steroid Biochemistry","raw_type":"journal-article"},{"id":"pmid:2770301","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2770301","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of steroid biochemistry","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1688330120","display_name":null,"funder_award_id":"1-K04-HD-00697","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G1735474802","display_name":"SOMATOMEDIN C NOVEL REGULATOR OF GRANULOSA CELL FUNCTION","funder_award_id":"5k04hd000697-02","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G4891997262","display_name":null,"funder_award_id":"HD-19998","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G874568285","display_name":null,"funder_award_id":"AM 074222","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1566852849","https://openalex.org/W1930538850","https://openalex.org/W1978755614","https://openalex.org/W2015526883","https://openalex.org/W2025897094","https://openalex.org/W2027768019","https://openalex.org/W2029980846","https://openalex.org/W2036648552","https://openalex.org/W2042761114","https://openalex.org/W2046865955","https://openalex.org/W2049016611","https://openalex.org/W2067630278","https://openalex.org/W2094603311","https://openalex.org/W2103269531","https://openalex.org/W2201985778"],"related_works":["https://openalex.org/W2007136533","https://openalex.org/W2015526883","https://openalex.org/W2047684740","https://openalex.org/W2063984668","https://openalex.org/W2002257675","https://openalex.org/W2105589316","https://openalex.org/W2043305112","https://openalex.org/W2025897094","https://openalex.org/W2080142236","https://openalex.org/W2042833466"],"abstract_inverted_index":null,"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}