{"id":"https://openalex.org/W2010348159","doi":"https://doi.org/10.1271/bbb.61.359","title":"Substrate Specificities of\u03b1-Galactosidases from Yeasts","display_name":"Substrate Specificities of\u03b1-Galactosidases from Yeasts","publication_year":1997,"publication_date":"1997-01-01","ids":{"openalex":"https://openalex.org/W2010348159","doi":"https://doi.org/10.1271/bbb.61.359","mag":"2010348159","pmid":"https://pubmed.ncbi.nlm.nih.gov/9058977"},"language":"en","primary_location":{"id":"doi:10.1271/bbb.61.359","doi":"https://doi.org/10.1271/bbb.61.359","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.61.359","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019198667","display_name":"Shigeki Yoshida","orcid":"https://orcid.org/0000-0002-5559-6783","observed_orcids":["https://orcid.org/0000-0002-5559-6783"]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shigeki Yoshida","raw_affiliation_strings":["Institute of Applied Biochemistry, University of Tsukuba, Japan","Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biochemistry, University of Tsukuba, Japan","institution_ids":["https://openalex.org/I146399215"]},{"raw_affiliation_string":"Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036739823","display_name":"Chin Hui Tan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Chin Hui Tan","raw_affiliation_strings":["Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101572001","display_name":"Tomoko Shimokawa","orcid":"https://orcid.org/0000-0003-2843-2422","observed_orcids":["https://orcid.org/0000-0003-2843-2422"]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tomoko Shimokawa","raw_affiliation_strings":["Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005711013","display_name":"Hilkka Turakainen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hilkka Turakainen","raw_affiliation_strings":["Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110380508","display_name":"Isao Kusakabe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Isao Kusakabe","raw_affiliation_strings":["Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Biochemistry, University of Tsukuba, 1\u20131\u20131 Tennoodai, Tsukuba-shi, Ibaraki 305, Japan","institution_ids":["https://openalex.org/I146399215"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I146399215"],"apc_list":{"value":3272,"currency":"USD","value_usd":3272},"apc_paid":null,"fwci":0.7715,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.61310395,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"61","issue":"2","first_page":"359","last_page":"361"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.006099999882280827,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-galactosidase","display_name":"alpha-galactosidase","score":0.9670000076293945},{"id":"https://openalex.org/keywords/yeast","display_name":"yeast","score":0.640999972820282},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.9789999723434448},{"id":"https://openalex.org/keywords/galactose-utilization","display_name":"galactose utilization","score":0.921999990940094},{"id":"https://openalex.org/keywords/melibiose","display_name":"melibiose","score":0.9350000023841858},{"id":"https://openalex.org/keywords/raffinose","display_name":"raffinose","score":1.0},{"id":"https://openalex.org/keywords/galactomannan","display_name":"galactomannan","score":0.6190000176429749},{"id":"https://openalex.org/keywords/intracellular-enzymes","display_name":"intracellular enzymes","score":0.5960000157356262},{"id":"https://openalex.org/keywords/extracellular-enzymes","display_name":"extracellular enzymes","score":0.890999972820282}],"concepts":[{"id":"https://openalex.org/C2778139943","wikidata":"https://www.wikidata.org/wiki/Q74411440","display_name":"Melibiose","level":4,"score":0.8105298280715942},{"id":"https://openalex.org/C2779998883","wikidata":"https://www.wikidata.org/wiki/Q410005","display_name":"Raffinose","level":3,"score":0.7784997224807739},{"id":"https://openalex.org/C2778173381","wikidata":"https://www.wikidata.org/wiki/Q66589593","display_name":"Galactose","level":2,"score":0.7317132353782654},{"id":"https://openalex.org/C2777249184","wikidata":"https://www.wikidata.org/wiki/Q5517842","display_name":"Galactosidases","level":5,"score":0.7196121215820312},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6742343902587891},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.6281523704528809},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5844312310218811},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.5632398128509521},{"id":"https://openalex.org/C64943373","wikidata":"https://www.wikidata.org/wiki/Q2651003","display_name":"Alpha (finance)","level":4,"score":0.5191947221755981},{"id":"https://openalex.org/C2776788019","wikidata":"https://www.wikidata.org/wiki/Q83170235","display_name":"Alpha-galactosidase","level":4,"score":0.4934138357639313},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.4676762521266937},{"id":"https://openalex.org/C2994592520","wikidata":"https://www.wikidata.org/wiki/Q5090496","display_name":"Substrate specificity","level":3,"score":0.44151192903518677},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4377972185611725},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34919023513793945},{"id":"https://openalex.org/C43053761","wikidata":"https://www.wikidata.org/wiki/Q286379","display_name":"Beta-galactosidase","level":4,"score":0.27016907930374146},{"id":"https://openalex.org/C2778597767","wikidata":"https://www.wikidata.org/wiki/Q4027534","display_name":"Sucrose","level":2,"score":0.15351274609565735},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.06831642985343933},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.05319333076477051},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.0},{"id":"https://openalex.org/C2777404818","wikidata":"https://www.wikidata.org/wiki/Q615645","display_name":"Fabry disease","level":3,"score":0.0},{"id":"https://openalex.org/C2775944032","wikidata":"https://www.wikidata.org/wiki/Q22907659","display_name":"Patient satisfaction","level":2,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C49453240","wikidata":"https://www.wikidata.org/wiki/Q1592163","display_name":"Construct validity","level":3,"score":0.0},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.0},{"id":"https://openalex.org/C159110408","wikidata":"https://www.wikidata.org/wiki/Q121176","display_name":"Nursing","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000519","descriptor_name":"alpha-Galactosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009598","descriptor_name":"Nitrophenylgalactosides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015003","descriptor_name":"Yeasts","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1271/bbb.61.359","doi":"https://doi.org/10.1271/bbb.61.359","is_oa":false,"landing_page_url":"https://doi.org/10.1271/bbb.61.359","pdf_url":null,"source":{"id":"https://openalex.org/S94095188","display_name":"Bioscience Biotechnology and Biochemistry","issn_l":"0916-8451","issn":["0916-8451","1347-6947"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, Biotechnology, and Biochemistry","raw_type":"journal-article"},{"id":"pmid:9058977","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9058977","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioscience, biotechnology, and biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":3,"referenced_works":["https://openalex.org/W208501556","https://openalex.org/W1512734204","https://openalex.org/W1583133340"],"related_works":["https://openalex.org/W2000500126","https://openalex.org/W2068936897","https://openalex.org/W2088541091","https://openalex.org/W2369983775","https://openalex.org/W2005410118","https://openalex.org/W2042700482","https://openalex.org/W2979805961","https://openalex.org/W4246465345","https://openalex.org/W6958885479","https://openalex.org/W2064926044"],"abstract_inverted_index":{"Twenty-nine":[0],"strains":[1],"of":[2,7,35],"yeasts,":[3],"which":[4],"are":[5],"capable":[6],"using":[8,40],"galactose,":[9],"melibiose,":[10],"or":[11],"raffinose,":[12],"were":[13,38],"screened":[14],"for":[15],"alpha-galactosidase":[16],"production.":[17],"Among":[18],"the":[19,47,55],"strains,":[20],"5":[21],"produced":[22,29],"intracellular":[23,31],"and":[24,27,42],"extracellular":[25],"alpha-galactosidases,":[26],"2":[28],"only":[30],"enzyme.":[32],"Substrate":[33],"specificities":[34],"these":[36],"enzymes":[37,45],"explored":[39],"6(3)-alpha-D-galactosyl-1,4-beta-D-mannotriose":[41],"6(3)-alpha-D-galactosyl-1,4-beta-D-mannotetraose.":[43,59],"All":[44],"liberated":[46],"terminal":[48],"galactose":[49,57],"from":[50,58],"6(3)-alpha-D-galactosyl-1,4-beta-D-mannotriose,":[51],"but":[52],"did":[53],"not":[54],"stub":[56]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}