{"id":"https://openalex.org/W2010304843","doi":"https://doi.org/10.1002/pro.625","title":"Monitoring the conformational changes of an intrinsically disordered peptide using a quartz crystal microbalance","display_name":"Monitoring the conformational changes of an intrinsically disordered peptide using a quartz crystal microbalance","publication_year":2011,"publication_date":"2011-03-17","ids":{"openalex":"https://openalex.org/W2010304843","doi":"https://doi.org/10.1002/pro.625","mag":"2010304843","pmid":"https://pubmed.ncbi.nlm.nih.gov/21416544","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3125875"},"language":"en","primary_location":{"id":"doi:10.1002/pro.625","doi":"https://doi.org/10.1002/pro.625","is_oa":true,"landing_page_url":"https://doi.org/10.1002/pro.625","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/pro.625","source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/pro.625","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034442265","display_name":"Oren Shur","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Oren Shur","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, New York, New York, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Jun Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jun Wu","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, New York, New York","Department of Chemical Engineering, Columbia University, New York, New York#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York#TAB#","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056535522","display_name":"Donald M. Cropek","orcid":"https://orcid.org/0000-0002-6312-5578","observed_orcids":["https://orcid.org/0000-0002-6312-5578"]},"institutions":[{"id":"https://openalex.org/I4400009010","display_name":"Construction Engineering Research Laboratory","ror":"https://ror.org/003mzyg61","country_code":"US","type":"facility","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1306490931","https://openalex.org/I1330347796","https://openalex.org/I4400009010","https://openalex.org/I87303767"]},{"id":"https://openalex.org/I87303767","display_name":"U.S. Army Engineer Research and Development Center","ror":"https://ror.org/027mhn368","country_code":"US","type":"facility","lineage":["https://openalex.org/I1304082316","https://openalex.org/I1306490931","https://openalex.org/I1330347796","https://openalex.org/I87303767"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Donald M. Cropek","raw_affiliation_strings":["U.S. Army Engineer Research and Development Center, Construction Engineering Research Laboratory (CERL), Champaign, Illinois"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"U.S. Army Engineer Research and Development Center, Construction Engineering Research Laboratory (CERL), Champaign, Illinois","institution_ids":["https://openalex.org/I4400009010","https://openalex.org/I87303767"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063109716","display_name":"Scott A. Banta","orcid":"https://orcid.org/0000-0001-7885-0150","observed_orcids":["https://orcid.org/0000-0001-7885-0150"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Scott Banta","raw_affiliation_strings":["Department of Chemical Engineering, Columbia University, New York, New York","Department of Chemical Engineering, Columbia University, New York, New York#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York#TAB#","institution_ids":["https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5063109716"],"corresponding_institution_ids":["https://openalex.org/I78577930"],"apc_list":{"value":4480,"currency":"USD","value_usd":4480},"apc_paid":null,"fwci":0.7783,"has_fulltext":true,"cited_by_count":19,"citation_normalized_percentile":{"value":0.68806901,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"20","issue":"5","first_page":"925","last_page":"930"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intrinsically-disordered-peptides","display_name":"intrinsically disordered peptides","score":0.996999979019165},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.7850000262260437},{"id":"https://openalex.org/keywords/calcium-binding","display_name":"calcium binding","score":0.7059999704360962},{"id":"https://openalex.org/keywords/peptide-immobilization","display_name":"peptide immobilization","score":0.6660000085830688},{"id":"https://openalex.org/keywords/biosensors","display_name":"biosensors","score":0.6859999895095825}],"concepts":[{"id":"https://openalex.org/C9996572","wikidata":"https://www.wikidata.org/wiki/Q629569","display_name":"Quartz crystal microbalance","level":3,"score":0.8181519508361816},{"id":"https://openalex.org/C2777339483","wikidata":"https://www.wikidata.org/wiki/Q647203","display_name":"Conformational change","level":2,"score":0.6768654584884644},{"id":"https://openalex.org/C2778815515","wikidata":"https://www.wikidata.org/wiki/Q3408242","display_name":"Intrinsically disordered proteins","level":2,"score":0.6750022768974304},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.634042501449585},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5861571431159973},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5307982563972473},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.501176118850708},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.41034364700317383},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.2605695128440857},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20973461866378784},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000262","descriptor_name":"Adenylyl Cyclases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001886","descriptor_name":"Bordetella pertussis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D002942","descriptor_name":"Circular Dichroism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058525","descriptor_name":"Quartz Crystal Microbalance Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/pro.625","doi":"https://doi.org/10.1002/pro.625","is_oa":true,"landing_page_url":"https://doi.org/10.1002/pro.625","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/pro.625","source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3125875","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3125875","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Sci","raw_type":"Text"},{"id":"pmid:21416544","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21416544","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein science : a publication of the Protein Society","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/pro.625","doi":"https://doi.org/10.1002/pro.625","is_oa":true,"landing_page_url":"https://doi.org/10.1002/pro.625","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/pro.625","source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2010304843.pdf","grobid_xml":"https://content.openalex.org/works/W2010304843.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W1988456728","https://openalex.org/W2013593586","https://openalex.org/W2022959668","https://openalex.org/W2028129929","https://openalex.org/W2032433791","https://openalex.org/W2041918990","https://openalex.org/W2044730187","https://openalex.org/W2049994974","https://openalex.org/W2056399792","https://openalex.org/W2063471184","https://openalex.org/W2073712544","https://openalex.org/W2073745520","https://openalex.org/W2091383694","https://openalex.org/W2092153867","https://openalex.org/W2101953406","https://openalex.org/W2109045926","https://openalex.org/W2111433307","https://openalex.org/W2121635781","https://openalex.org/W2125022522","https://openalex.org/W2128055699","https://openalex.org/W2133691154","https://openalex.org/W2143727927","https://openalex.org/W2144318457","https://openalex.org/W2145383898","https://openalex.org/W2317194131","https://openalex.org/W4210968583"],"related_works":["https://openalex.org/W2063471184","https://openalex.org/W2277806122","https://openalex.org/W2090250822","https://openalex.org/W2326571398","https://openalex.org/W2407334313","https://openalex.org/W2039028859","https://openalex.org/W2321107817","https://openalex.org/W1980358684","https://openalex.org/W2900892624","https://openalex.org/W2807887191"],"abstract_inverted_index":{"Intrinsically":[0],"disordered":[1,51],"peptides":[2],"(IDPs)":[3],"have":[4,91,126],"recently":[5],"garnered":[6],"much":[7],"interest":[8],"because":[9],"of":[10,33,42,62,87,105,159,169,180],"their":[11,21],"role":[12],"in":[13,59,129,142,146,152],"biological":[14],"processes":[15],"such":[16],"as":[17,161],"molecular":[18],"recognition":[19],"and":[20,84,174,188,194],"ability":[22],"to":[23,53,76,101,124,139,148,164],"undergo":[24],"stimulus-responsive":[25],"conformational":[26,82,167],"changes.":[27],"The":[28],"block":[29],"V":[30],"repeat-in-toxin":[31],"motif":[32],"the":[34,60,81,102,116,135,143,149,157,166],"Bordetella":[35],"pertussis":[36],"adenylate":[37],"cyclase":[38],"is":[39,71],"an":[40,43,54],"example":[41],"IDP":[44],"that":[45],"undergoes":[46],"a":[47,50,67,106,162,178],"transition":[48,75],"from":[49],"state":[52],"ordered":[55],"beta":[56],"roll":[57],"conformation":[58],"presence":[61],"calcium":[63],"ions.":[64],"In":[65,131],"solution,":[66],"C-terminal":[68],"capping":[69],"domain":[70],"necessary":[72],"for":[73,99,177],"this":[74,88],"occur.":[77],"To":[78],"further":[79],"explore":[80],"behavior":[83,150],"folding":[85],"requirements":[86],"IDP,":[89],"we":[90],"cysteine":[92],"modified":[93],"three":[94],"previously":[95],"characterized":[96],"constructs,":[97],"allowing":[98],"attachment":[100],"gold":[103],"surface":[104,137],"quartz":[107],"crystal":[108],"microbalance":[109],"(QCM).":[110],"We":[111],"demonstrate":[112],"that,":[113],"while":[114],"immobilized,":[115],"C-terminally":[117],"capped":[118],"peptide":[119],"exhibits":[120],"similar":[121],"calcium-binding":[122],"properties":[123],"what":[125],"been":[127],"observed":[128,151],"solution.":[130,153],"addition,":[132],"immobilization":[133],"on":[134,172],"solid":[136],"appears":[138],"enable":[140],"calcium-responsiveness":[141],"uncapped":[144],"peptides,":[145],"contrast":[147],"This":[154],"work":[155],"demonstrates":[156],"power":[158],"QCM":[160],"tool":[163],"study":[165],"changes":[168],"IDPs":[170,184],"immobilized":[171],"surfaces":[173],"has":[175],"implications":[176],"range":[179],"potential":[181],"applications":[182],"where":[183],"may":[185],"be":[186],"engineered":[187],"used":[189],"including":[190],"protein":[191],"purification,":[192],"biosensors,":[193],"other":[195],"bionanotechnology":[196],"applications.":[197]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}