{"id":"https://openalex.org/W2010218490","doi":"https://doi.org/10.1111/j.1365-2818.1983.tb04174.x","title":"Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii\u2010cells for electron microscopy","display_name":"Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawii\u2010cells for electron microscopy","publication_year":1983,"publication_date":"1983-02-01","ids":{"openalex":"https://openalex.org/W2010218490","doi":"https://doi.org/10.1111/j.1365-2818.1983.tb04174.x","mag":"2010218490","pmid":"https://pubmed.ncbi.nlm.nih.gov/6405040"},"language":"en","primary_location":{"id":"doi:10.1111/j.1365-2818.1983.tb04174.x","doi":"https://doi.org/10.1111/j.1365-2818.1983.tb04174.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-2818.1983.tb04174.x","pdf_url":null,"source":{"id":"https://openalex.org/S168975578","display_name":"Journal of Microscopy","issn_l":"0022-2720","issn":["0022-2720","1365-2818"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Microscopy","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082239812","display_name":"Claes Weibull","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I187531555","display_name":"Lund University","ror":"https://ror.org/012a77v79","country_code":"SE","type":"education","lineage":["https://openalex.org/I187531555"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"C. Weibull","raw_affiliation_strings":["Department of Microbiology, University of Lund, So\u0308lvegatan 21, S-223 62 Lund, Sweden","Department of Microbiology, University of Lund, S\u00f6lvegatan 21, S\u2010223 62 Lund, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Lund, So\u0308lvegatan 21, S-223 62 Lund, Sweden","institution_ids":["https://openalex.org/I187531555"]},{"raw_affiliation_string":"Department of Microbiology, University of Lund, S\u00f6lvegatan 21, S\u2010223 62 Lund, Sweden","institution_ids":["https://openalex.org/I187531555"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014939970","display_name":"Anders Christiansson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I187531555","display_name":"Lund University","ror":"https://ror.org/012a77v79","country_code":"SE","type":"education","lineage":["https://openalex.org/I187531555"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"A. Christiansson","raw_affiliation_strings":["Department of Microbiology, University of Lund, So\u0308lvegatan 21, S-223 62 Lund, Sweden","Department of Microbiology, University of Lund, S\u00f6lvegatan 21, S\u2010223 62 Lund, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, University of Lund, So\u0308lvegatan 21, S-223 62 Lund, Sweden","institution_ids":["https://openalex.org/I187531555"]},{"raw_affiliation_string":"Department of Microbiology, University of Lund, S\u00f6lvegatan 21, S\u2010223 62 Lund, Sweden","institution_ids":["https://openalex.org/I187531555"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111531346","display_name":"Eric Carlemalm","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1850255","display_name":"University of Basel","ror":"https://ror.org/02s6k3f65","country_code":"CH","type":"education","lineage":["https://openalex.org/I1850255"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"E. Carlemalm","raw_affiliation_strings":["Department of Microbiology, Biocentre, University of Basle, Klingelbergstrasse 70, CH-4056 Basle, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Biocentre, University of Basle, Klingelbergstrasse 70, CH-4056 Basle, Switzerland","institution_ids":["https://openalex.org/I1850255"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4760,"currency":"USD","value_usd":4760},"apc_paid":null,"fwci":3.8652,"has_fulltext":false,"cited_by_count":91,"citation_normalized_percentile":{"value":0.94288305,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"129","issue":"2","first_page":"201","last_page":"207"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9595000147819519,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9595000147819519,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.03449999913573265,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acholeplasma-laidlawii","display_name":"Acholeplasma laidlawii","score":0.9800000190734863},{"id":"https://openalex.org/keywords/plasma-membrane","display_name":"plasma membrane","score":0.9129999876022339},{"id":"https://openalex.org/keywords/glutaraldehyde-fixation","display_name":"glutaraldehyde fixation","score":0.8949999809265137},{"id":"https://openalex.org/keywords/osmium-tetroxide-fixation","display_name":"osmium tetroxide fixation","score":0.9729999899864197},{"id":"https://openalex.org/keywords/uranyl-acetate","display_name":"uranyl acetate","score":0.878000020980835},{"id":"https://openalex.org/keywords/dehydration-temperature","display_name":"dehydration temperature","score":0.6679999828338623},{"id":"https://openalex.org/keywords/lowicryl-k4m","display_name":"Lowicryl K4M","score":0.9559999704360962},{"id":"https://openalex.org/keywords/epon-embedding","display_name":"Epon embedding","score":0.824999988079071},{"id":"https://openalex.org/keywords/fatty-acid-saturation","display_name":"fatty acid saturation","score":0.7099999785423279}],"concepts":[{"id":"https://openalex.org/C2779424567","wikidata":"https://www.wikidata.org/wiki/Q422021","display_name":"Osmium tetroxide","level":3,"score":0.9684368968009949},{"id":"https://openalex.org/C2777529624","wikidata":"https://www.wikidata.org/wiki/Q416475","display_name":"Glutaraldehyde","level":2,"score":0.9400149583816528},{"id":"https://openalex.org/C2781060271","wikidata":"https://www.wikidata.org/wiki/Q421620","display_name":"Uranyl acetate","level":3,"score":0.9018157720565796},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6371608972549438},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.5804563164710999},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.5749768018722534},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5708507895469666},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5622447729110718},{"id":"https://openalex.org/C2780161600","wikidata":"https://www.wikidata.org/wiki/Q153","display_name":"Ethanol","level":2,"score":0.5466994047164917},{"id":"https://openalex.org/C2778769901","wikidata":"https://www.wikidata.org/wiki/Q194290","display_name":"Dehydration","level":2,"score":0.5410110354423523},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.49665719270706177},{"id":"https://openalex.org/C540285154","wikidata":"https://www.wikidata.org/wiki/Q751","display_name":"Osmium","level":4,"score":0.4610333740711212},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.4045674204826355},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3005238175392151},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.2471705973148346},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.23455777764320374},{"id":"https://openalex.org/C555196967","wikidata":"https://www.wikidata.org/wiki/Q1086","display_name":"Ruthenium","level":3,"score":0.0},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000128","descriptor_name":"Acholeplasma laidlawii","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D000128","descriptor_name":"Acholeplasma laidlawii","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D000180","descriptor_name":"Acrylic Resins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003890","descriptor_name":"Desiccation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005404","descriptor_name":"Fixatives","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005976","descriptor_name":"Glutaral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008563","descriptor_name":"Membrane Lipids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D009942","descriptor_name":"Organometallic Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009993","descriptor_name":"Osmium Tetroxide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013048","descriptor_name":"Specimen Handling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014501","descriptor_name":"Uranium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1365-2818.1983.tb04174.x","doi":"https://doi.org/10.1111/j.1365-2818.1983.tb04174.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1365-2818.1983.tb04174.x","pdf_url":null,"source":{"id":"https://openalex.org/S168975578","display_name":"Journal of Microscopy","issn_l":"0022-2720","issn":["0022-2720","1365-2818"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Microscopy","raw_type":"journal-article"},{"id":"pmid:6405040","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6405040","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of microscopy","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320323741","display_name":"Magnus Bergvalls Stiftelse","ror":"https://ror.org/040ya8k73"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W1645044045","https://openalex.org/W1983890053","https://openalex.org/W1990646719","https://openalex.org/W2018778009","https://openalex.org/W2033058478","https://openalex.org/W2037022053","https://openalex.org/W2099292414","https://openalex.org/W2141576462","https://openalex.org/W2318343261"],"related_works":["https://openalex.org/W1994010185","https://openalex.org/W2062975178","https://openalex.org/W1965667673","https://openalex.org/W3035971465","https://openalex.org/W2318343261","https://openalex.org/W3036823261","https://openalex.org/W1983909575","https://openalex.org/W2417764065","https://openalex.org/W2007526152","https://openalex.org/W1977190090"],"abstract_inverted_index":{"SUMMARY":[0],"The":[1,70,144,225,263,282],"aim":[2],"of":[3,33,44,56,98,135,139,201,215,218,222,227,233,248],"the":[4,10,42,54,57,60,67,136,140,159,162,202,213,219,223,238,280,295],"present":[5],"investigation":[6],"was":[7,103,142,146,156,168,198,258,284],"to":[8,12,129,148,292],"study":[9],"extent":[11],"which":[13],"lipids":[14,43,61],"are":[15,62],"extracted":[16,266],"from":[17,267],"biological":[18],"membranes":[19],"during":[20,100],"dehydration":[21,255],"and":[22,59,83,91,110,120,161,177,187,256],"embedding":[23,257],"procedures":[24,102],"carried":[25],"out":[26],"at":[27,74,117,124,184,192,260],"high":[28],"or":[29,86],"low":[30],"temperatures.":[31],"Cells":[32,240],"Acholeplasma":[34],"laidlawii":[35],"were":[36,72,113,180,297],"used":[37],"as":[38,229],"experimental":[39],"material,":[40],"since":[41],"this":[45,81],"bacterium":[46],"easily":[47],"can":[48],"be":[49],"radioactively":[50],"labelled":[51],"without":[52],"labelling":[53],"rest":[55],"cell,":[58],"almost":[63],"entirely":[64],"located":[65],"in":[66,94,122,158,189,212,279],"cytoplasmic":[68],"membrane.":[69],"cells":[71,106,141,173,269],"fixed":[73,107,174,241],"277":[75],"K":[76,186],"with":[77,80,87,108,115,153,175,182,243],"glutaraldehyde,":[78,88],"sequentially":[79],"reagent":[82],"osmium":[84,89,111,178,287],"tetroxide,":[85],"tetroxide":[90,112,179],"uranyl":[92,154],"acetate":[93,155],"that":[95,277],"order.":[96],"Loss":[97],"lipid":[99,137,197,220,250,264,293],"these":[101],"negligible.":[104],"When":[105,172],"glutaraldehyde":[109,176,244],"dehydrated":[114,181],"ethanol":[116,183,234],"room":[118,193],"temperature":[119],"embedded":[121,188],"Epon":[123],"333":[125],"K,":[126],"i.e.":[127],"subjected":[128],"a":[130],"conventional":[131],"treatment,":[132],"about":[133,216,246],"90%":[134],"content":[138,221],"extracted.":[143,199],"loss":[145,214],"reduced":[147],"c.":[149],"20%":[150],"when":[151,253],"treatment":[152],"included":[157],"procedure":[160],"non\u2010polar":[163],"methacrylate":[164],"resin":[165,205],"Lowicryl":[166,190,206,209],"HM20":[167,191,210],"substituted":[169],"for":[170,208,286],"Epon.":[171],"238":[185,261],"temperature,":[194],"practically":[195],"no":[196],"Substitution":[200],"polar":[203],"methacrylate\u2010acrylate":[204],"K4M":[207],"resulted":[211],"half":[217,247],"cells.":[224,281,289],"use":[226],"ethanediol":[228],"dehydrating":[230],"agent":[231],"instead":[232],"did":[235],"not":[236],"diminish":[237],"extraction.":[239],"solely":[242],"lost":[245],"their":[249],"content,":[251],"even":[252],"both":[254],"performed":[259],"K.":[262],"material":[265],"glutaraldehyde\u2010fixed":[268],"contained":[270],"slightly":[271],"more":[272],"saturated":[273],"fatty":[274],"acids":[275],"than":[276],"remaining":[278],"reverse":[283],"true":[285],"tetroxide\u2010fixed":[288],"With":[290],"respect":[291],"species,":[294],"extractions":[296],"generally":[298],"rather":[299],"unspecific.":[300]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}
