{"id":"https://openalex.org/W2009852157","doi":"https://doi.org/10.1097/01.brs.0000184558.44874.c0","title":"Exposure to Pulsed Low Intensity Ultrasound Stimulates Extracellular Matrix Metabolism of Bovine Intervertebral Disc Cells Cultured in Alginate Beads","display_name":"Exposure to Pulsed Low Intensity Ultrasound Stimulates Extracellular Matrix Metabolism of Bovine Intervertebral Disc Cells Cultured in Alginate Beads","publication_year":2005,"publication_date":"2005-10-26","ids":{"openalex":"https://openalex.org/W2009852157","doi":"https://doi.org/10.1097/01.brs.0000184558.44874.c0","mag":"2009852157","pmid":"https://pubmed.ncbi.nlm.nih.gov/16261116"},"language":"en","primary_location":{"id":"doi:10.1097/01.brs.0000184558.44874.c0","doi":"https://doi.org/10.1097/01.brs.0000184558.44874.c0","is_oa":false,"landing_page_url":"https://doi.org/10.1097/01.brs.0000184558.44874.c0","pdf_url":null,"source":{"id":"https://openalex.org/S4026894","display_name":"Spine","issn_l":"0362-2436","issn":["0362-2436","1528-1159"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Spine","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009557393","display_name":"Kei Miyamoto","orcid":"https://orcid.org/0000-0001-6400-5140","observed_orcids":["https://orcid.org/0000-0001-6400-5140"]},"institutions":[{"id":"https://openalex.org/I1285301757","display_name":"Rush University Medical Center","ror":"https://ror.org/01j7c0b24","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285301757"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kei Miyamoto","raw_affiliation_strings":["Department of Orthopedic Surgery, Medical College, Rush University Medical Center, Chicago, IL, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedic Surgery, Medical College, Rush University Medical Center, Chicago, IL, USA","institution_ids":["https://openalex.org/I1285301757"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069437689","display_name":"Howard S. An","orcid":"https://orcid.org/0000-0001-9560-9131","observed_orcids":["https://orcid.org/0000-0001-9560-9131"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Howard S. An","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026261610","display_name":"Robert L. Sah","orcid":"https://orcid.org/0000-0002-5493-9502","observed_orcids":["https://orcid.org/0000-0002-5493-9502"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert L. Sah","raw_affiliation_strings":["Department of Bioengineering & Whitaker Institute of Biomedical Engineering, University of California-San Diego, La Jolla, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering & Whitaker Institute of Biomedical Engineering, University of California-San Diego, La Jolla, CA","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038213232","display_name":"Koji Akeda","orcid":"https://orcid.org/0000-0001-9468-9387","observed_orcids":["https://orcid.org/0000-0001-9468-9387"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Koji Akeda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109290617","display_name":"Masahiko Okuma","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Masahiko Okuma","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025516742","display_name":"Lori Otten","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Lori Otten","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109948648","display_name":"Eugene J.\u2010M.A. Thonar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Eugene J.M.A. Thonar","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5000662848","display_name":"Koichi Masuda","orcid":"https://orcid.org/0000-0002-5361-4415","observed_orcids":["https://orcid.org/0000-0002-5361-4415"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Koichi Masuda","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5000662848","https://openalex.org/A5009557393","https://openalex.org/A5025516742","https://openalex.org/A5038213232","https://openalex.org/A5069437689","https://openalex.org/A5109290617","https://openalex.org/A5109948648"],"corresponding_institution_ids":["https://openalex.org/I1285301757"],"apc_list":null,"apc_paid":null,"fwci":1.5397,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.81252856,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"30","issue":"21","first_page":"2398","last_page":"2405"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10238","display_name":"Spine and Intervertebral Disc Pathology","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10238","display_name":"Spine and Intervertebral Disc Pathology","score":0.9696999788284302,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12249","display_name":"Veterinary Orthopedics and Neurology","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intervertebral-disc","display_name":"intervertebral disc","score":0.8550000190734863},{"id":"https://openalex.org/keywords/extracellular-matrix-metabolism","display_name":"extracellular matrix metabolism","score":0.9829999804496765},{"id":"https://openalex.org/keywords/nucleus-pulposus","display_name":"nucleus pulposus","score":0.8550000190734863},{"id":"https://openalex.org/keywords/anulus-fibrosus","display_name":"anulus fibrosus","score":0.9929999709129333},{"id":"https://openalex.org/keywords/proteoglycan-synthesis","display_name":"proteoglycan synthesis","score":0.925000011920929},{"id":"https://openalex.org/keywords/collagen-synthesis","display_name":"collagen synthesis","score":1.0},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.7229999899864197},{"id":"https://openalex.org/keywords/alginate-beads","display_name":"alginate beads","score":0.7300000190734863},{"id":"https://openalex.org/keywords/intervertebral-disc-tissue-engineering","display_name":"intervertebral disc tissue engineering","score":0.8460000157356262}],"concepts":[{"id":"https://openalex.org/C2778550726","wikidata":"https://www.wikidata.org/wiki/Q3658544","display_name":"Low-intensity pulsed ultrasound","level":4,"score":0.8568387627601624},{"id":"https://openalex.org/C2779335624","wikidata":"https://www.wikidata.org/wiki/Q407976","display_name":"Proteoglycan","level":3,"score":0.7250854969024658},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.6475675106048584},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.6165747046470642},{"id":"https://openalex.org/C2777755357","wikidata":"https://www.wikidata.org/wiki/Q502781","display_name":"Intervertebral disc","level":2,"score":0.5960226058959961},{"id":"https://openalex.org/C143753070","wikidata":"https://www.wikidata.org/wiki/Q162564","display_name":"Ultrasound","level":2,"score":0.5605679154396057},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.48823192715644836},{"id":"https://openalex.org/C2780550940","wikidata":"https://www.wikidata.org/wiki/Q502730","display_name":"Cartilage","level":2,"score":0.45861318707466125},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3614184856414795},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.35359451174736023},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.277397096157074},{"id":"https://openalex.org/C2779727089","wikidata":"https://www.wikidata.org/wiki/Q564875","display_name":"Therapeutic ultrasound","level":3,"score":0.23595494031906128},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.16587728261947632},{"id":"https://openalex.org/C126838900","wikidata":"https://www.wikidata.org/wiki/Q77604","display_name":"Radiology","level":1,"score":0.06416371464729309}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003050","descriptor_name":"Coccyx","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D003094","descriptor_name":"Collagen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005109","descriptor_name":"Extracellular Matrix","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007403","descriptor_name":"Intervertebral Disc","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D007403","descriptor_name":"Intervertebral Disc","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011509","descriptor_name":"Proteoglycans","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014464","descriptor_name":"Ultrasonic Therapy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1097/01.brs.0000184558.44874.c0","doi":"https://doi.org/10.1097/01.brs.0000184558.44874.c0","is_oa":false,"landing_page_url":"https://doi.org/10.1097/01.brs.0000184558.44874.c0","pdf_url":null,"source":{"id":"https://openalex.org/S4026894","display_name":"Spine","issn_l":"0362-2436","issn":["0362-2436","1528-1159"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Spine","raw_type":"journal-article"},{"id":"pmid:16261116","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16261116","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Spine","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G57766917","display_name":"SPECIALIZED CENTER OF RESEARCH IN OSTEOARTHRITIS","funder_award_id":"P50AR039239","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G8877839404","display_name":"Program Project grant","funder_award_id":"P01AR048152","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"}],"funders":[{"id":"https://openalex.org/F4320310956","display_name":"Smith and Nephew","ror":"https://ror.org/03agge938"},{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W179376564","https://openalex.org/W186063252","https://openalex.org/W1569612555","https://openalex.org/W1617781531","https://openalex.org/W1897804960","https://openalex.org/W1965282660","https://openalex.org/W1968708020","https://openalex.org/W1970093515","https://openalex.org/W1979233208","https://openalex.org/W1980095106","https://openalex.org/W1986868734","https://openalex.org/W1987512190","https://openalex.org/W1996584907","https://openalex.org/W2000071455","https://openalex.org/W2002201489","https://openalex.org/W2002236075","https://openalex.org/W2007155550","https://openalex.org/W2010681836","https://openalex.org/W2021495896","https://openalex.org/W2036264639","https://openalex.org/W2036763406","https://openalex.org/W2037998434","https://openalex.org/W2045932857","https://openalex.org/W2051039353","https://openalex.org/W2056732606","https://openalex.org/W2058907148","https://openalex.org/W2058932182","https://openalex.org/W2071484745","https://openalex.org/W2075143033","https://openalex.org/W2078850755","https://openalex.org/W2085821199","https://openalex.org/W2087115556","https://openalex.org/W2089190379","https://openalex.org/W2093415150","https://openalex.org/W2106755981","https://openalex.org/W2113961735","https://openalex.org/W2120134923","https://openalex.org/W2129331493","https://openalex.org/W2149714830","https://openalex.org/W2155586598","https://openalex.org/W2168733054","https://openalex.org/W2180067096","https://openalex.org/W2271221255","https://openalex.org/W2333692367","https://openalex.org/W2334161627","https://openalex.org/W2406721410","https://openalex.org/W4242058998","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W1995704747","https://openalex.org/W2285913012","https://openalex.org/W2145902878","https://openalex.org/W2183780311","https://openalex.org/W2289015919","https://openalex.org/W1969423356","https://openalex.org/W83607105","https://openalex.org/W7095532981","https://openalex.org/W2320482694","https://openalex.org/W2351380503"],"abstract_inverted_index":{"STUDY":[0],"DESIGN:":[1],"In":[2,108],"vitro":[3],"study":[4],"on":[5,13,32,74],"the":[6,14,67,84,109,119,136,161,189,200,255,261,297],"effects":[7,31,68],"of":[8,17,50,69,83,142,163,168,202,224,254,263,276,300,306],"pulsed":[9,26,51,70,112,203,225,264,277],"low":[10,27,52,71,113,204,226,265,278,290],"intensity":[11,28,53,72,114,205,227,266,279,291],"ultrasound":[12,29,54,73,110,115,206,228,267,280,292],"cellular":[15],"metabolism":[16,38,284,302],"bovine":[18,40,97],"intervertebral":[19,41,75,256,286,307],"disc":[20,42,76,257,287,308],"cells.":[21,43,288],"OBJECTIVE:":[22],"To":[23],"determine":[24],"whether":[25],"has":[30],"cell":[33,181,301],"proliferation":[34],"and":[35,63,87,89,100,149,152,158,172,183,210,212,237],"extracellular":[36,282],"matrix":[37,283],"by":[39,199,247,260],"SUMMARY":[44],"OF":[45],"BACKGROUND":[46],"DATA:":[47],"The":[48,130,222,274],"application":[49,201,223,262,275],"is":[55],"known":[56],"to":[57,118],"be":[58],"effective":[59],"in":[60,102,135,180,207,232,285],"stimulating":[61],"fracture":[62],"cartilage":[64],"repair.":[65],"However,":[66],"cells":[77,216,241,248],"are":[78],"not":[79],"known.":[80],"METHODS:":[81],"Cells":[82],"nucleus":[85,208],"pulposus":[86,209],"inner":[88,211,236],"outer":[90,213,238],"anulus":[91,214,239],"fibrosus":[92,215,240],"were":[93,165,186],"enzymatically":[94],"isolated":[95,249],"from":[96,250],"coccygeal":[98],"tissue":[99,304],"precultured":[101],"alginate":[103,233],"beads":[104,234],"for":[105,121,125,296,303],"14":[106],"days.":[107,129],"group,":[111],"was":[116,133,174,197,258],"administered":[117],"culture":[120],"20":[122,128,159],"minutes":[123],"daily":[124],"an":[126],"additional":[127],"control":[131],"group":[132],"cultured":[134],"same":[137],"way":[138],"but":[139],"without":[140],"administration":[141],"ultrasound.":[143],"Cell":[144],"viability,":[145],"DNA":[146,184],"content,":[147],"proteoglycan":[148,153,173,195,230],"collagen":[150,171],"synthesis,":[151],"content":[154,185,231],"at":[155],"days":[156],"10":[157],"after":[160],"initiation":[162],"treatment":[164],"evaluated.":[166],"Characterization":[167],"newly":[169],"synthesized":[170],"performed.":[175],"RESULTS:":[176],"No":[177],"significant":[178],"differences":[179],"viability":[182],"observed":[187],"between":[188],"two":[190],"groups.":[191],"On":[192],"day":[193],"20,":[194],"synthesis":[196,246],"increased":[198,229,259],"(24%-26%":[217],"increase,":[218,269],"P":[219,270],"<":[220,243,271],"0.001).":[221],"containing":[235],"(P":[242],"0.05).":[244],"Collagen":[245],"all":[251],"three":[252],"zones":[253],"(16%-19%":[268],"0.05-0.0001).":[272],"CONCLUSIONS:":[273],"stimulated":[281],"Pulsed":[289],"may":[293],"prove":[294],"useful":[295],"physical":[298],"stimulation":[299],"engineering":[305],"tissue.":[309]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}