{"id":"https://openalex.org/W2009763597","doi":"https://doi.org/10.1021/ac071159y","title":"Single-Molecule Detection of Surface-Hybridized Human Papilloma Virus DNA for Quantitative Clinical Screening","display_name":"Single-Molecule Detection of Surface-Hybridized Human Papilloma Virus DNA for Quantitative Clinical Screening","publication_year":2007,"publication_date":"2007-10-03","ids":{"openalex":"https://openalex.org/W2009763597","doi":"https://doi.org/10.1021/ac071159y","mag":"2009763597","pmid":"https://pubmed.ncbi.nlm.nih.gov/17914754"},"language":"en","primary_location":{"id":"doi:10.1021/ac071159y","doi":"https://doi.org/10.1021/ac071159y","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac071159y","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2009763597"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100447456","display_name":"Ji\u2010Young Lee","orcid":"https://orcid.org/0000-0002-7631-5127","observed_orcids":["https://orcid.org/0000-0002-7631-5127"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]},{"id":"https://openalex.org/I2802789608","display_name":"Ames National Laboratory","ror":"https://ror.org/041m9xr71","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I173911158","https://openalex.org/I2802789608","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ji-Young Lee","raw_affiliation_strings":["Ames LaboratoryUSDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011","Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames LaboratoryUSDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158","https://openalex.org/I2802789608"]},{"raw_affiliation_string":"Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA","institution_ids":["https://openalex.org/I173911158","https://openalex.org/I2802789608"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101794952","display_name":"Jiangwei Li","orcid":"https://orcid.org/0000-0002-0207-5554","observed_orcids":["https://orcid.org/0000-0002-0207-5554"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]},{"id":"https://openalex.org/I2802789608","display_name":"Ames National Laboratory","ror":"https://ror.org/041m9xr71","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I173911158","https://openalex.org/I2802789608","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jiangwei Li","raw_affiliation_strings":["Ames LaboratoryUSDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames LaboratoryUSDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158","https://openalex.org/I2802789608"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5061282830","display_name":"Edward S. Yeung","orcid":"https://orcid.org/0000-0003-0919-6700","observed_orcids":["https://orcid.org/0000-0003-0919-6700"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]},{"id":"https://openalex.org/I2802789608","display_name":"Ames National Laboratory","ror":"https://ror.org/041m9xr71","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I173911158","https://openalex.org/I2802789608","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Edward S. Yeung","raw_affiliation_strings":["Ames LaboratoryUSDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ames LaboratoryUSDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011","institution_ids":["https://openalex.org/I173911158","https://openalex.org/I2802789608"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5061282830"],"corresponding_institution_ids":["https://openalex.org/I173911158","https://openalex.org/I2802789608"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.9188,"has_fulltext":false,"cited_by_count":40,"citation_normalized_percentile":{"value":0.85009805,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"79","issue":"21","first_page":"8083","last_page":"8089"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9154999852180481,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9154999852180481,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10146","display_name":"Cervical Cancer and HPV Research","score":0.03959999978542328,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.018799999728798866,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-papillomavirus","display_name":"human papillomavirus","score":0.7670000195503235},{"id":"https://openalex.org/keywords/hpv-16","display_name":"HPV16","score":0.8759999871253967},{"id":"https://openalex.org/keywords/single-molecule-detection","display_name":"single-molecule detection","score":0.8799999952316284},{"id":"https://openalex.org/keywords/viral-dna-quantification","display_name":"viral DNA quantification","score":0.8270000219345093},{"id":"https://openalex.org/keywords/surface-hybridization","display_name":"surface hybridization","score":0.6240000128746033},{"id":"https://openalex.org/keywords/fluorescent-probes","display_name":"fluorescent probes","score":0.4180000126361847},{"id":"https://openalex.org/keywords/cervical-cells","display_name":"cervical cells","score":0.625},{"id":"https://openalex.org/keywords/pap-test","display_name":"Pap smear","score":0.8709999918937683},{"id":"https://openalex.org/keywords/viral-load","display_name":"viral load","score":0.5720000267028809},{"id":"https://openalex.org/keywords/cervical-cancer-screening","display_name":"cervical cancer screening","score":0.5910000205039978}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7340491414070129},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7087071537971497},{"id":"https://openalex.org/C130197536","wikidata":"https://www.wikidata.org/wiki/Q418436","display_name":"Hybridization probe","level":3,"score":0.6234042048454285},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6226704120635986},{"id":"https://openalex.org/C137390916","wikidata":"https://www.wikidata.org/wiki/Q900923","display_name":"DNA\u2013DNA hybridization","level":3,"score":0.5499493479728699},{"id":"https://openalex.org/C54871578","wikidata":"https://www.wikidata.org/wiki/Q2074275","display_name":"Nucleic acid thermodynamics","level":4,"score":0.5133363604545593},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.44969305396080017},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.446927011013031},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.42652034759521484},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.419491171836853},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.24342882633209229},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.17644476890563965},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14461737871170044},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.13711071014404297},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10384982824325562},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.09213066101074219},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.07501992583274841}],"mesh":[{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008403","descriptor_name":"Mass Screening","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013499","descriptor_name":"Surface Properties","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015344","descriptor_name":"DNA Probes, HPV","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/ac071159y","doi":"https://doi.org/10.1021/ac071159y","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac071159y","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:17914754","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17914754","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306084","display_name":"U.S. Department of Energy","ror":"https://ror.org/01bj3aw27"},{"id":"https://openalex.org/F4320311090","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337480","display_name":"Basic Energy Sciences","ror":"https://ror.org/05mg91w61"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W204171487","https://openalex.org/W1671829508","https://openalex.org/W1974648751","https://openalex.org/W1988679339","https://openalex.org/W1989509271","https://openalex.org/W1999404175","https://openalex.org/W2009744204","https://openalex.org/W2010430693","https://openalex.org/W2041523376","https://openalex.org/W2042193909","https://openalex.org/W2047480444","https://openalex.org/W2050717506","https://openalex.org/W2090864621","https://openalex.org/W2094814209","https://openalex.org/W2100644020","https://openalex.org/W2102310519","https://openalex.org/W2105184358","https://openalex.org/W2140100637","https://openalex.org/W2140535402","https://openalex.org/W2148198035","https://openalex.org/W2148790544","https://openalex.org/W2170801441"],"related_works":["https://openalex.org/W2074601794","https://openalex.org/W4205572703","https://openalex.org/W1481549466","https://openalex.org/W58799254","https://openalex.org/W1966544525","https://openalex.org/W2301632944","https://openalex.org/W1980280593","https://openalex.org/W2394536440","https://openalex.org/W1992292530","https://openalex.org/W1549119427"],"abstract_inverted_index":{"We":[0],"present":[1],"an":[2],"improved":[3],"method":[4,174],"to":[5,24,30,74,92,117,124,162,165,197,215],"quantify":[6],"viral":[7,142],"DNA":[8,21,50,71,144,159],"in":[9,95],"human":[10],"cells":[11,184,218],"at":[12],"the":[13,42,45,57,75,80,83,101,104,133,140,150,192,206],"single-":[14],"molecule":[15],"level.":[16],"Human":[17],"papilloma":[18],"virus":[19],"(HPV)-16":[20],"was":[22,63,86,90,155,175,194],"hybridized":[23],"probes":[25],"that":[26,65,93],"were":[27,51,122,185,212],"covalently":[28],"bound":[29],"a":[31,37,60,67,96,136,179],"glass":[32],"surface":[33],"and":[34,48,132,160,188,191],"detected":[35],"with":[36,128,139,149,157,168,178,200,219],"single-molecule":[38],"imaging":[39],"system.":[40],"In":[41,56],"single-probe":[43,81],"mode,":[44,59],"whole":[46],"genome":[47],"target":[49],"fluorescently":[52,68],"labeled":[53,69],"before":[54],"hybridization.":[55,198],"dual-probe":[58,102,173],"second":[61],"probe":[62,77],"introduced":[64],"has":[66],"1-kb":[70],"strand":[72],"connected":[73],"50-nt":[76],"sequence.":[78],"With":[79,100],"method,":[82,103,154],"detection":[84],"limit":[85],"0.7":[87],"copy/cell,":[88],"which":[89,111],"similar":[91],"reported":[94,141],"flow":[97],"system":[98],"earlier.":[99],"linear":[105],"dynamic":[106],"range":[107],"covers":[108],"1.44-7000":[109],"copies/cell,":[110],"is":[112],"typical":[113],"of":[114,208],"early":[115],"infection":[116],"near-cancer":[118],"stages.":[119],"Both":[120],"methods":[121],"applied":[123],"cell":[125],"line":[126],"samples":[127],"known":[129],"HPV-16":[130,158],"infection,":[131],"result":[134],"showed":[135],"good":[137],"match":[138],"load.":[143],"from":[145,222],"cervical":[146],"cells,":[147],"collected":[148],"Pap":[151],"smear":[152],"sampling":[153,170],"spiked":[156],"submitted":[161,196],"this":[163],"assay":[164],"show":[166],"compatibility":[167],"conventional":[169],"methods.":[171],"The":[172,183],"further":[176],"tested":[177],"crudely":[180],"prepared":[181],"sample.":[182],"heat":[186],"lyzed":[187],"spun":[189],"down,":[190],"supernatant":[193],"immediately":[195],"Even":[199],"reduced":[201],"hybridization":[202],"efficiency":[203],"caused":[204],"by":[205],"interference":[207],"cellular":[209],"materials,":[210],"we":[211],"still":[213],"able":[214],"differentiate":[216],"infected":[217],"600":[220],"copies/cell":[221],"healthy":[223],"cells.":[224]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
