{"id":"https://openalex.org/W2009461998","doi":"https://doi.org/10.1186/1743-422x-6-90","title":"Development and Validation of a HPV-32 Specific PCR Assay","display_name":"Development and Validation of a HPV-32 Specific PCR Assay","publication_year":2009,"publication_date":"2009-06-27","ids":{"openalex":"https://openalex.org/W2009461998","doi":"https://doi.org/10.1186/1743-422x-6-90","mag":"2009461998","pmid":"https://pubmed.ncbi.nlm.nih.gov/19558697","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2715387"},"language":"en","primary_location":{"id":"doi:10.1186/1743-422x-6-90","doi":"https://doi.org/10.1186/1743-422x-6-90","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1743-422x-6-90","pdf_url":"https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-6-90","source":{"id":"https://openalex.org/S115714813","display_name":"Virology Journal","issn_l":"1743-422X","issn":["1743-422X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Virology Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-6-90","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056364683","display_name":"Nicholas R. Herrel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75420490","display_name":"Louisiana State University Health Sciences Center New Orleans","ror":"https://ror.org/01qv8fp92","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121820613","https://openalex.org/I75420490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas R Herrel","raw_affiliation_strings":["Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, USA. nherre@lsuhsc.edu","Dept. of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, USA. nherre@lsuhsc.edu","institution_ids":["https://openalex.org/I75420490"]},{"raw_affiliation_string":"Dept. of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, USA","institution_ids":["https://openalex.org/I75420490"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Nadia L Johnson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75420490","display_name":"Louisiana State University Health Sciences Center New Orleans","ror":"https://ror.org/01qv8fp92","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121820613","https://openalex.org/I75420490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nadia L Johnson","raw_affiliation_strings":["Department of Medicine, Louisiana State, University Health Sciences Center, New Orleans, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Louisiana State, University Health Sciences Center, New Orleans, USA","institution_ids":["https://openalex.org/I75420490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018443257","display_name":"Jennifer E. Cameron","orcid":"https://orcid.org/0000-0002-5785-2410","observed_orcids":["https://orcid.org/0000-0002-5785-2410"]},"institutions":[{"id":"https://openalex.org/I114832834","display_name":"Tulane University","ror":"https://ror.org/04vmvtb21","country_code":"US","type":"education","lineage":["https://openalex.org/I114832834"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennifer E Cameron","raw_affiliation_strings":["Cancer Center, Tulane University Health Sciences Center, New Orleans, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cancer Center, Tulane University Health Sciences Center, New Orleans, USA","institution_ids":["https://openalex.org/I114832834"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108479894","display_name":"Janet E. Leigh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75420490","display_name":"Louisiana State University Health Sciences Center New Orleans","ror":"https://ror.org/01qv8fp92","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121820613","https://openalex.org/I75420490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Janet Leigh","raw_affiliation_strings":["Department of Oral Medicine, Louisiana State University Health Sciences Center, New Orleans, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral Medicine, Louisiana State University Health Sciences Center, New Orleans, USA","institution_ids":["https://openalex.org/I75420490"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110429218","display_name":"Michael E. Hagensee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I75420490","display_name":"Louisiana State University Health Sciences Center New Orleans","ror":"https://ror.org/01qv8fp92","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121820613","https://openalex.org/I75420490"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Michael E Hagensee","raw_affiliation_strings":["Department of Medicine, Louisiana State, University Health Sciences Center, New Orleans, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Louisiana State, University Health Sciences Center, New Orleans, USA","institution_ids":["https://openalex.org/I75420490"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5110429218"],"corresponding_institution_ids":["https://openalex.org/I75420490"],"apc_list":{"value":3290,"currency":"USD","value_usd":3290},"apc_paid":{"value":3290,"currency":"USD","value_usd":3290},"fwci":0.0,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.16093388,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"6","issue":"1","first_page":"90","last_page":"90"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10146","display_name":"Cervical Cancer and HPV Research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10146","display_name":"Cervical Cancer and HPV Research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13761","display_name":"HIV/AIDS oral health manifestations","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10307","display_name":"Head and Neck Cancer Studies","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2733","display_name":"Otorhinolaryngology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hpv-32","display_name":"HPV 32","score":0.9300000071525574},{"id":"https://openalex.org/keywords/human-papillomavirus","display_name":"human papillomavirus","score":0.8539999723434448},{"id":"https://openalex.org/keywords/l1-gene","display_name":"L1 gene","score":0.6740000247955322},{"id":"https://openalex.org/keywords/oral-warts","display_name":"oral warts","score":0.718999981880188},{"id":"https://openalex.org/keywords/people-living-with-hiv","display_name":"people living with HIV","score":0.8949999809265137},{"id":"https://openalex.org/keywords/dot-blot-hybridization","display_name":"dot blot","score":0.9330000281333923},{"id":"https://openalex.org/keywords/hpv-42","display_name":"HPV42","score":0.8899999856948853},{"id":"https://openalex.org/keywords/oral-swabs","display_name":"oral swabs","score":0.6200000047683716},{"id":"https://openalex.org/keywords/natural-history-of-hpv-infection","display_name":"natural history of HPV infection","score":0.8769999742507935}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8363973498344421},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7042425870895386},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4500008225440979}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D030361","descriptor_name":"Papillomavirus Infections","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1186/1743-422x-6-90","doi":"https://doi.org/10.1186/1743-422x-6-90","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1743-422x-6-90","pdf_url":"https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-6-90","source":{"id":"https://openalex.org/S115714813","display_name":"Virology Journal","issn_l":"1743-422X","issn":["1743-422X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Virology Journal","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:1866273","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2715387","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:2715387","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2715387","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Virol J","raw_type":"Text"},{"id":"pmid:19558697","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19558697","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Virology journal","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.355.7987","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.355.7987","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/0e/90/Virol_J_2009_Jun_27_6_90.tar.gz","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:b6eee73158bb4bccb2a68d0207451bee","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/b6eee73158bb4bccb2a68d0207451bee","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Virology Journal, Vol 6, Iss 1, p 90 (2009)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/1743-422x-6-90","doi":"https://doi.org/10.1186/1743-422x-6-90","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1743-422x-6-90","pdf_url":"https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-6-90","source":{"id":"https://openalex.org/S115714813","display_name":"Virology Journal","issn_l":"1743-422X","issn":["1743-422X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Virology Journal","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9789000153541565}],"study_designs":[],"awards":[{"id":"https://openalex.org/G287975954","display_name":"Prevalence of HPV in the Oral Cavity of HIV+ Individuals","funder_award_id":"5r21de015051-02","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G4197882860","display_name":"Mentoring Oral Health Research in Louisiana","funder_award_id":"P20RR020160","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G5145822358","display_name":null,"funder_award_id":"5R21 DE015051","funder_id":"https://openalex.org/F4320337365","funder_display_name":"National Institute of Dental and Craniofacial Research"},{"id":"https://openalex.org/G6273715767","display_name":null,"funder_award_id":"1R03 CA11132","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6872812553","display_name":null,"funder_award_id":"R21 DE015051","funder_id":"https://openalex.org/F4320337365","funder_display_name":"National Institute of Dental and Craniofacial Research"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337365","display_name":"National Institute of Dental and Craniofacial Research","ror":"https://ror.org/004a2wv92"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2009461998.pdf","grobid_xml":"https://content.openalex.org/works/W2009461998.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W629972863","https://openalex.org/W1860504974","https://openalex.org/W1974894607","https://openalex.org/W1977661325","https://openalex.org/W1980316863","https://openalex.org/W2000286559","https://openalex.org/W2009081571","https://openalex.org/W2013403745","https://openalex.org/W2015666655","https://openalex.org/W2064632813","https://openalex.org/W2073432782","https://openalex.org/W2077768908","https://openalex.org/W2093677661","https://openalex.org/W2133818906","https://openalex.org/W2151164686","https://openalex.org/W2408285184","https://openalex.org/W3128343078"],"related_works":["https://openalex.org/W7096919590","https://openalex.org/W2147996012","https://openalex.org/W206488534","https://openalex.org/W2023614043","https://openalex.org/W7098402553","https://openalex.org/W2096010288","https://openalex.org/W2093905793","https://openalex.org/W3093547006","https://openalex.org/W2390083709","https://openalex.org/W2057137879"],"abstract_inverted_index":{"BACKGROUND:":[0],"Human":[1],"Papillomavirus-32":[2],"(HPV-32)":[3],"has":[4,166],"traditionally":[5],"been":[6],"associated":[7],"with":[8,89],"focal-epithelial-hyperplasia":[9],"(FEH).":[10],"It":[11],"is":[12,25,192,227,233],"also":[13],"present":[14],"in":[15,239],"58%":[16],"of":[17,20,32,41,127,144,146,169,237,253,259],"oral":[18,260],"warts":[19],"HIV-positive":[21,113],"individuals":[22],"whose":[23],"prevalence":[24],"increasing.":[26],"Current":[27],"methods":[28],"for":[29,83,235],"the":[30,39,97,132,151,158,161,195,210,217,222,250,257],"detection":[31,84,145,236],"HPV-32":[33,64,95,117,128,152,162,196,204,223,238,254],"are":[34],"labor-intensive":[35],"and":[36,50,71,75,86,109,137,171,175,178,182,186,207,220,232,256],"insensitive":[37],"so":[38],"goal":[40],"this":[42],"work":[43],"was":[44,69,77,180],"to":[45,52,79,124,131,194,201,216,229,248],"develop":[46],"a":[47,142,167],"highly":[48],"sensitive":[49],"easy":[51],"use":[53],"specific":[54,66,118,163,197,224],"polymerase":[55],"chain":[56],"reaction":[57],"(PCR)":[58],"assay.":[59],"MATERIALS":[60],"AND":[61],"METHODS:":[62],"An":[63],"L1":[65,101],"PCR":[67,88,119,136,164,198,225],"assay":[68,120,156,165,226,243],"developed":[70],"optimized.":[72],"The":[73,116,189],"sensitivity":[74,123,168,191],"specificity":[76,179,221],"compared":[78,130],"previous":[80,230],"assays":[81,199,231],"utilized":[82],"(PGMY":[85],"MY09/11":[87,135],"dot":[90,138,153],"blot":[91,139,154],"hybridization)":[92],"using":[93],"cloned":[94],"L1,":[96],"closely":[98],"related":[99],"HPV-42":[100],"as":[102,104,129,157],"well":[103],"clinical":[105],"samples":[106,206],"(oral":[107],"swabs":[108],"fluids":[110],"from":[111],"89":[112],"subjects).":[114],"RESULTS:":[115],"showed":[121],"improved":[122],"5":[125],"copies":[126],"PGMY":[133],"PCR,":[134],"which":[140],"had":[141],"limit":[143],"approximately":[147],"3,000":[148],"copies.":[149],"Using":[150],"hybridization":[155],"gold":[159,212],"standard,":[160],"95.8%":[170],"88.9%":[172],"by":[173,184],"sample":[174,185],"subject,":[176,187],"respectively,":[177],"87.8%":[181],"58.8%":[183],"respectively.":[188],"low":[190],"due":[193],"ability":[200],"detect":[202],"more":[203],"positive":[205],"may":[208],"be":[209],"new":[211],"standard.":[213],"CONCLUSION:":[214],"Due":[215],"ease,":[218],"sensitivity,":[219],"superior":[228],"ideal":[234],"large":[240],"cohorts.":[241],"This":[242],"provides":[244],"an":[245],"excellent":[246],"tool":[247],"study":[249],"natural":[251],"history":[252],"infection":[255],"development":[258],"warts.":[261]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
