{"id":"https://openalex.org/W2009102102","doi":"https://doi.org/10.1039/b110988j","title":"Coumarins give misleading absorbance with Ellman\u2019s reagent suggestive of thiol conjugates","display_name":"Coumarins give misleading absorbance with Ellman\u2019s reagent suggestive of thiol conjugates","publication_year":2002,"publication_date":"2002-01-30","ids":{"openalex":"https://openalex.org/W2009102102","doi":"https://doi.org/10.1039/b110988j","mag":"2009102102","pmid":"https://pubmed.ncbi.nlm.nih.gov/11996355"},"language":"en","primary_location":{"id":"doi:10.1039/b110988j","doi":"https://doi.org/10.1039/b110988j","is_oa":false,"landing_page_url":"https://doi.org/10.1039/b110988j","pdf_url":null,"source":{"id":"https://openalex.org/S105746325","display_name":"The Analyst","issn_l":"0003-2654","issn":["0003-2654","1364-5528"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Analyst","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://edoc.mpg.de/33892","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078141671","display_name":"Maik Berlich","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Maik Berlich","raw_affiliation_strings":["Martin-Luther-University Halle-Wittenberg, Dept Biochemistry/Biotechnology, Institute of Biochemistry, Halle/S, Germany","Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Martin-Luther-University Halle-Wittenberg, Dept Biochemistry/Biotechnology, Institute of Biochemistry, Halle/S, Germany","institution_ids":["https://openalex.org/I68956291"]},{"raw_affiliation_string":"Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086916036","display_name":"Sieglinde Menge","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sieglinde Menge","raw_affiliation_strings":["Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019898626","display_name":"I. Bruns","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ina Bruns","raw_affiliation_strings":["Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany","institution_ids":["https://openalex.org/I68956291"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102122900","display_name":"J\u00fcergen Schmidt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210110463","display_name":"Leibniz Institute of Plant Biochemistry","ror":"https://ror.org/01mzk5576","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210110463"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Juergen Schmidt","raw_affiliation_strings":["Institute of Plant Biochemistry, Weinberg 3, 06120 Halle/Saale, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Plant Biochemistry, Weinberg 3, 06120 Halle/Saale, Germany","institution_ids":["https://openalex.org/I4210110463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078146869","display_name":"Bernd Schneider","orcid":"https://orcid.org/0000-0003-4788-1565","observed_orcids":["https://orcid.org/0000-0003-4788-1565"]},"institutions":[{"id":"https://openalex.org/I149899117","display_name":"Max Planck Society","ror":"https://ror.org/01hhn8329","country_code":"DE","type":"nonprofit","lineage":["https://openalex.org/I149899117"]},{"id":"https://openalex.org/I4210117675","display_name":"Max Planck Institute for Chemical Ecology","ror":"https://ror.org/02ks53214","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210117675"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Bernd Schneider","raw_affiliation_strings":["Max Planck Institute for Chemical Ecology, Beutenberg-Campus, 07745 Jena, Germany","Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Max Planck Institute for Chemical Ecology, Beutenberg-Campus, 07745 Jena, Germany","institution_ids":["https://openalex.org/I4210117675"]},{"raw_affiliation_string":"Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society","institution_ids":["https://openalex.org/I149899117","https://openalex.org/I4210117675"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111746021","display_name":"Gerd\u2010Joachim Krauss","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I68956291","display_name":"Martin Luther University Halle-Wittenberg","ror":"https://ror.org/05gqaka33","country_code":"DE","type":"education","lineage":["https://openalex.org/I68956291"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Gerd-Joachim Krauss","raw_affiliation_strings":["Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Martin-Luther-University Halle-Wittenberg, Dept. Biochemistry/Biotechnology, Institute of Biochemistry, Kurt-Mothes-Str. 3, 06120 Halle/S, Germany","institution_ids":["https://openalex.org/I68956291"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5111746021"],"corresponding_institution_ids":["https://openalex.org/I68956291"],"apc_list":null,"apc_paid":null,"fwci":0.3476,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.44278858,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"127","issue":"3","first_page":"333","last_page":"336"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11481","display_name":"Sulfur Compounds in Biology","score":0.0982000008225441,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11481","display_name":"Sulfur Compounds in Biology","score":0.0982000008225441,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11973","display_name":"Plant chemical constituents analysis","score":0.09440000355243683,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13079","display_name":"Heavy Metals in Plants","score":0.09380000084638596,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/coumarins","display_name":"coumarins","score":0.9829999804496765},{"id":"https://openalex.org/keywords/phytochelatins","display_name":"phytochelatins","score":0.8970000147819519},{"id":"https://openalex.org/keywords/ellman-s-reagent","display_name":"Ellman's reagent","score":0.9210000038146973},{"id":"https://openalex.org/keywords/dtnb","display_name":"DTNB","score":0.7390000224113464},{"id":"https://openalex.org/keywords/thiol-detection","display_name":"thiol detection","score":0.8109999895095825},{"id":"https://openalex.org/keywords/cadmium-stress","display_name":"cadmium stress","score":0.8309999704360962},{"id":"https://openalex.org/keywords/polytrichum-formosum","display_name":"Polytrichum formosum","score":0.8730000257492065},{"id":"https://openalex.org/keywords/atrichum-undulatum","display_name":"Atrichum undulatum","score":1.0},{"id":"https://openalex.org/keywords/terrestrial-mosses","display_name":"terrestrial mosses","score":0.7910000085830688},{"id":"https://openalex.org/keywords/glutathione","display_name":"glutathione","score":0.8050000071525574},{"id":"https://openalex.org/keywords/uv-vis-absorbance","display_name":"UV-Vis absorbance","score":0.671999990940094}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.869094967842102},{"id":"https://openalex.org/C2781324293","wikidata":"https://www.wikidata.org/wiki/Q220410","display_name":"Thiol","level":2,"score":0.8222249746322632},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.6952052712440491},{"id":"https://openalex.org/C2776923573","wikidata":"https://www.wikidata.org/wiki/Q2294884","display_name":"Derivatization","level":3,"score":0.6867285966873169},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.5627010464668274},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5128908157348633},{"id":"https://openalex.org/C98015330","wikidata":"https://www.wikidata.org/wiki/Q907315","display_name":"Absorbance","level":2,"score":0.49997401237487793},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.47779709100723267},{"id":"https://openalex.org/C197404232","wikidata":"https://www.wikidata.org/wiki/Q319827","display_name":"Chelation","level":2,"score":0.4595876932144165},{"id":"https://openalex.org/C2781338262","wikidata":"https://www.wikidata.org/wiki/Q111812","display_name":"Coumarin","level":2,"score":0.4306175112724304},{"id":"https://openalex.org/C544657597","wikidata":"https://www.wikidata.org/wiki/Q1091","display_name":"Cadmium","level":2,"score":0.42412126064300537},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.24160507321357727},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.05513182282447815}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1039/b110988j","doi":"https://doi.org/10.1039/b110988j","is_oa":false,"landing_page_url":"https://doi.org/10.1039/b110988j","pdf_url":null,"source":{"id":"https://openalex.org/S105746325","display_name":"The Analyst","issn_l":"0003-2654","issn":["0003-2654","1364-5528"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Analyst","raw_type":"journal-article"},{"id":"pmid:11996355","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11996355","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Analyst","raw_type":"Journal Article"},{"id":"pmh:oai:edoc.mpg.de:33892","doi":null,"is_oa":true,"landing_page_url":"http://edoc.mpg.de/33892","pdf_url":null,"source":{"id":"https://openalex.org/S4406922265","display_name":"Max Planck Institute for Plasma Physics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Analyst, v.127, 333-336 (2002)","raw_type":"Article"},{"id":"pmh:oai:pure.mpg.de:item_442423","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/11858/00-001M-0000-0012-B5A6-C","pdf_url":null,"source":{"id":"https://openalex.org/S4306400654","display_name":"MPG.PuRe (Max Planck Society)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I149899117","host_organization_name":"Max Planck Society","host_organization_lineage":["https://openalex.org/I149899117"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Analyst","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"pmh:oai:edoc.mpg.de:33892","doi":null,"is_oa":true,"landing_page_url":"http://edoc.mpg.de/33892","pdf_url":null,"source":{"id":"https://openalex.org/S4406922265","display_name":"Max Planck Institute for Plasma Physics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Analyst, v.127, 333-336 (2002)","raw_type":"Article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1985591693","https://openalex.org/W1998450090","https://openalex.org/W2028840350","https://openalex.org/W2044671576","https://openalex.org/W2051583593","https://openalex.org/W2060843940","https://openalex.org/W2084173021","https://openalex.org/W2095514509","https://openalex.org/W2118150573","https://openalex.org/W2159085671"],"related_works":["https://openalex.org/W4390469493","https://openalex.org/W2580890603","https://openalex.org/W1993329826","https://openalex.org/W2114264507","https://openalex.org/W2020587775","https://openalex.org/W2075834169","https://openalex.org/W1998277992","https://openalex.org/W2005286522","https://openalex.org/W1992763173","https://openalex.org/W4239606225"],"abstract_inverted_index":{"In":[0,80],"the":[1,12,34,56,69,76,84,111,122,164,170,191],"course":[2],"of":[3,58,68,78,86,124,136,158,193],"a":[4,140,143,183],"screening":[5],"for":[6,150,169,173],"phytochelatins":[7,31,51,89,116],"in":[8,11,53,75,196],"cadmium-exposed":[9],"bryophytes":[10],"terrestrial":[13],"mosses":[14],"Polytrichum":[15],"formosum":[16,103],"and":[17,26,40,104,127,153],"Atrichum":[18],"undulatum":[19,152],"we":[20,90,130],"detected":[21,112],"compounds":[22,60,113,195],"with":[23,43,50],"absorption":[24,180],"properties":[25],"retention":[27],"times":[28],"similar":[29],"to":[30,82],"when":[32],"applying":[33],"commonly":[35],"used":[36],"standard":[37],"method":[38],"RP-HPLC":[39],"post-column":[41,185],"derivatization":[42],"thiol-specific":[44],"DTNB":[45],"(Ellman)":[46],"reagent.":[47],"Moreover,":[48],"as":[49,94,96],"known":[52],"other":[54,118],"plants,":[55],"concentrations":[57],"these":[59,87,137],"increased":[61,74],"slightly":[62],"after":[63],"Cd":[64],"stress.":[65],"The":[66,156],"concentration":[67],"precursor":[70],"glutathione":[71],"(gamma-ECG),":[72],"however,":[73],"presence":[77,192],"Cd.":[79],"order":[81],"verify":[83],"identity":[85],"putative":[88],"performed":[91],"LC-ESI-MS":[92],"analyses":[93],"well":[95],"1H":[97],"NMR":[98,126],"on":[99],"extracts":[100],"from":[101],"P.":[102,154],"A.":[105,151],"undulatum.":[106],"Spectroscopic":[107],"investigations":[108,160],"indicated":[109],"that":[110,132,162],"were":[114],"neither":[115],"nor":[117],"thiol":[119,174],"compounds.":[120],"From":[121],"results":[123,157],"HPLC-1H":[125],"mass":[128],"spectrometry":[129],"concluded":[131],"at":[133],"least":[134],"one":[135],"substances":[138],"was":[139],"coumarin,":[141],"probably":[142],"5,8-dihydroxy-7-methoxycoumarin-5-beta-glucopyranoside,":[144],"which":[145],"has":[146],"already":[147],"been":[148],"described":[149],"formosum.":[155],"our":[159],"prove":[161,190],"under":[163],"basic":[165],"pH":[166],"conditions":[167],"essential":[168],"Ellman":[171,186],"test":[172],"compounds,":[175],"coumarins":[176],"show":[177],"comparable":[178],"UV/VIS":[179],"properties.":[181],"Therefore,":[182],"positive":[184],"reaction":[187],"cannot":[188],"unambiguously":[189],"thiol-containing":[194],"plants.":[197]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}