{"id":"https://openalex.org/W2008621986","doi":"https://doi.org/10.1021/ac100872x","title":"Peptide Fragmentation during Nanoelectrospray Ionization","display_name":"Peptide Fragmentation during Nanoelectrospray Ionization","publication_year":2010,"publication_date":"2010-07-07","ids":{"openalex":"https://openalex.org/W2008621986","doi":"https://doi.org/10.1021/ac100872x","mag":"2008621986","pmid":"https://pubmed.ncbi.nlm.nih.gov/20608645"},"language":"en","primary_location":{"id":"doi:10.1021/ac100872x","doi":"https://doi.org/10.1021/ac100872x","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac100872x","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100351705","display_name":"He Wang","orcid":"https://orcid.org/0000-0003-2987-8362","observed_orcids":["https://orcid.org/0000-0003-2987-8362","https://orcid.org/0009-0001-8114-5177"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"He Wang","raw_affiliation_strings":["Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2032, and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2032, and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063879522","display_name":"Zheng Ouyang","orcid":"https://orcid.org/0000-0002-7932-0933","observed_orcids":["https://orcid.org/0000-0002-7932-0933"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zheng Ouyang","raw_affiliation_strings":["Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2032, and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2032, and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062534695","display_name":"Yu Xia","orcid":"https://orcid.org/0000-0001-8694-9900","observed_orcids":["https://orcid.org/0000-0001-8694-9900"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Yu Xia","raw_affiliation_strings":["Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2032, and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907-2032, and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5062534695"],"corresponding_institution_ids":["https://openalex.org/I219193219"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.4405,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.5577567,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"82","issue":"15","first_page":"6534","last_page":"6541"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12776","display_name":"Electrohydrodynamics and Fluid Dynamics","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peptide-fragmentation","display_name":"peptide fragmentation","score":0.9129999876022339},{"id":"https://openalex.org/keywords/nanoelectrospray-ionization","display_name":"nanoelectrospray ionization","score":0.9940000176429749},{"id":"https://openalex.org/keywords/electrospray-ionization","display_name":"electrospray ionization","score":0.7649999856948853},{"id":"https://openalex.org/keywords/flow-rate","display_name":"flow rate","score":0.9100000262260437},{"id":"https://openalex.org/keywords/charged-droplets","display_name":"charged droplets","score":0.8230000138282776},{"id":"https://openalex.org/keywords/surface-electric-field","display_name":"surface electric field","score":0.8949999809265137},{"id":"https://openalex.org/keywords/collisional-activation","display_name":"collisional activation","score":0.8209999799728394},{"id":"https://openalex.org/keywords/phosphopeptides","display_name":"phosphopeptides","score":0.6809999942779541},{"id":"https://openalex.org/keywords/solvation","display_name":"solvation","score":0.6729999780654907}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9445592164993286},{"id":"https://openalex.org/C191015642","wikidata":"https://www.wikidata.org/wiki/Q1132459","display_name":"Fragmentation (computing)","level":2,"score":0.8811298608779907},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.7420279383659363},{"id":"https://openalex.org/C198291218","wikidata":"https://www.wikidata.org/wiki/Q190382","display_name":"Ionization","level":3,"score":0.6146072745323181},{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.581610381603241},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.4562791585922241},{"id":"https://openalex.org/C194451485","wikidata":"https://www.wikidata.org/wiki/Q1327575","display_name":"Electrospray","level":3,"score":0.42118164896965027},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.3296608030796051},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.257985383272171},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10102936625480652},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07626265287399292},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004553","descriptor_name":"Electric Conductivity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D010748","descriptor_name":"Phosphopeptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D036103","descriptor_name":"Nanotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/ac100872x","doi":"https://doi.org/10.1021/ac100872x","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac100872x","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:20608645","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20608645","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W334828138","https://openalex.org/W1706435611","https://openalex.org/W1973877615","https://openalex.org/W1974732006","https://openalex.org/W1974830441","https://openalex.org/W1979715681","https://openalex.org/W1979958714","https://openalex.org/W1984877442","https://openalex.org/W1990785897","https://openalex.org/W1996034128","https://openalex.org/W1998493296","https://openalex.org/W2002159977","https://openalex.org/W2004627688","https://openalex.org/W2010004076","https://openalex.org/W2017321877","https://openalex.org/W2023887263","https://openalex.org/W2024501626","https://openalex.org/W2024849484","https://openalex.org/W2028931227","https://openalex.org/W2031884770","https://openalex.org/W2032194486","https://openalex.org/W2033215287","https://openalex.org/W2036940947","https://openalex.org/W2037523684","https://openalex.org/W2038175847","https://openalex.org/W2046230650","https://openalex.org/W2047311903","https://openalex.org/W2047326594","https://openalex.org/W2048345443","https://openalex.org/W2056537276","https://openalex.org/W2073292063","https://openalex.org/W2078450003","https://openalex.org/W2080216143","https://openalex.org/W2080462561","https://openalex.org/W2081598183","https://openalex.org/W2088372814","https://openalex.org/W2095344572","https://openalex.org/W2113714398","https://openalex.org/W2135385298","https://openalex.org/W2145571626","https://openalex.org/W2163153896","https://openalex.org/W2171781071","https://openalex.org/W2644804608","https://openalex.org/W2975557304","https://openalex.org/W4251216540"],"related_works":["https://openalex.org/W2113714398","https://openalex.org/W1995070541","https://openalex.org/W3145133401","https://openalex.org/W2035942823","https://openalex.org/W2062062468","https://openalex.org/W2031884770","https://openalex.org/W4280488973","https://openalex.org/W2016072028","https://openalex.org/W2747663526","https://openalex.org/W2114785010"],"abstract_inverted_index":{"Electrospray":[0],"ionization":[1,7],"(ESI)":[2],"is":[3,15,167],"considered":[4],"a":[5,68,160],"soft":[6],"method,":[8],"and":[9,44,79,87,107,158,186],"typically":[10],"no":[11],"peptide":[12,26,54,120,171,195],"ion":[13],"fragmentation":[14,24,55,63],"observed.":[16,102],"Recently,":[17],"it":[18],"has":[19,58],"been":[20,59],"observed":[21,66],"that":[22,125,169],"intensive":[23],"of":[25,42,70,82,96,119,150,183,201,212],"ions":[27,172],"can":[28],"occur":[29],"during":[30,56,215],"nanoelectrospray":[31],"(nanoESI)":[32],"at":[33,159],"special":[34],"conditions,":[35],"such":[36],"as":[37,112],"solutions":[38,153],"containing":[39],"high":[40,138,176,199],"concentrations":[41],"salt":[43],"relatively":[45,198],"low":[46,161],"voltage":[47],"for":[48,67],"the":[49,80,97,113,117,180,187,194,204,210,213,216],"spray.":[50],"In":[51],"this":[52],"study,":[53],"nanoESI":[57,149],"systematically":[60],"characterized.":[61],"The":[62,197],"phenomenon":[64],"was":[65,101],"variety":[69],"peptides":[71],"with":[72,137,175],"molecular":[73],"weights":[74],"lower":[75],"than":[76],"3000":[77],"Da":[78],"types":[81],"fragments":[83],"include":[84],"a,":[85],"b,":[86],"y":[88],"ions.":[89],"For":[90],"phosphorylated":[91],"peptides,":[92],"very":[93,126],"little":[94],"loss":[95],"labile":[98],"phosphate":[99,205],"groups":[100,206],"Solution":[103],"electrical":[104],"conductivity":[105],"(K)":[106],"flow":[108,162],"rate":[109,163],"were":[110],"identified":[111],"key":[114],"parameters":[115],"affecting":[116],"degree":[118,200],"fragmentation.":[121,196],"Mechanistic":[122],"studies":[123],"suggested":[124],"fine":[127],"first":[128],"generation":[129],"charged":[130,184],"droplets":[131],"(approximately":[132,142],"30":[133],"nm":[134],"in":[135,191],"diameter)":[136],"surface":[139],"electric":[140],"field":[141],"1":[143],"V/nm)":[144],"could":[145],"be":[146],"formed":[147],"from":[148,179],"highly":[151],"conductive":[152],"(K":[154],"=":[155],"0.4":[156],"S/m)":[157],"(2":[164],"nL/min).":[165],"It":[166],"proposed":[168],"solvated":[170],"are":[173],"ejected":[174],"kinetic":[177],"energies":[178],"early":[181],"generations":[182],"droplets,":[185],"subsequent":[188],"collisional":[189],"activation":[190,217],"air":[192],"induces":[193],"solvation":[202],"around":[203],"may":[207],"contribute":[208],"to":[209],"preservation":[211],"phosphorylation":[214],"process.":[218]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}