{"id":"https://openalex.org/W2008367326","doi":"https://doi.org/10.1021/bi971181t","title":"Sialosyl-Fucosyl Poly-LacNAc without the Sialosyl-LexEpitope as the Physiological Myeloid Cell Ligand in E-Selectin-Dependent Adhesion: Studies under Static and Dynamic Flow Conditions,","display_name":"Sialosyl-Fucosyl Poly-LacNAc without the Sialosyl-LexEpitope as the Physiological Myeloid Cell Ligand in E-Selectin-Dependent Adhesion: Studies under Static and Dynamic Flow Conditions,","publication_year":1997,"publication_date":"1997-10-01","ids":{"openalex":"https://openalex.org/W2008367326","doi":"https://doi.org/10.1021/bi971181t","mag":"2008367326","pmid":"https://pubmed.ncbi.nlm.nih.gov/9376344"},"language":"en","primary_location":{"id":"doi:10.1021/bi971181t","doi":"https://doi.org/10.1021/bi971181t","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi971181t","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5020054555","display_name":"Kazuko Handa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kazuko Handa","raw_affiliation_strings":["Pacific Northwest Research Foundation, 720 Broadway, Seattle, Washington 98122, and Department of Pathobiology, University of Washington, Seattle, Washington 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Research Foundation, 720 Broadway, Seattle, Washington 98122, and Department of Pathobiology, University of Washington, Seattle, Washington 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113638228","display_name":"Mark R. Stroud","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark R. Stroud","raw_affiliation_strings":["Pacific Northwest Research Foundation, 720 Broadway, Seattle, Washington 98122, and Department of Pathobiology, University of Washington, Seattle, Washington 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Research Foundation, 720 Broadway, Seattle, Washington 98122, and Department of Pathobiology, University of Washington, Seattle, Washington 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070398233","display_name":"Sen\u2010itiroh Hakomori","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Sen-itiroh Hakomori","raw_affiliation_strings":["Pacific Northwest Research Foundation, 720 Broadway, Seattle, Washington 98122, and Department of Pathobiology, University of Washington, Seattle, Washington 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Research Foundation, 720 Broadway, Seattle, Washington 98122, and Department of Pathobiology, University of Washington, Seattle, Washington 98195","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5070398233"],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.084,"has_fulltext":false,"cited_by_count":69,"citation_normalized_percentile":{"value":0.87750732,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"36","issue":"41","first_page":"12412","last_page":"12420"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.8765000104904175,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.8765000104904175,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.11299999803304672,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.005200000014156103,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/e-selectin","display_name":"E-selectin","score":0.8880000114440918},{"id":"https://openalex.org/keywords/leukocyte-adhesion","display_name":"leukocyte adhesion","score":0.8460000157356262},{"id":"https://openalex.org/keywords/dynamic-flow-conditions","display_name":"dynamic flow conditions","score":0.7360000014305115},{"id":"https://openalex.org/keywords/hl-60-cells","display_name":"HL-60 cells","score":0.9269999861717224},{"id":"https://openalex.org/keywords/sialic-acid","display_name":"sialic acid","score":0.5260000228881836},{"id":"https://openalex.org/keywords/leukocyte-adhesion-molecules","display_name":"leukocyte adhesion molecules","score":0.39800000190734863}],"concepts":[{"id":"https://openalex.org/C2779212266","wikidata":"https://www.wikidata.org/wiki/Q17053974","display_name":"Fucosylation","level":4,"score":0.7663851976394653},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7027366757392883},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.6764460206031799},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.6030530333518982},{"id":"https://openalex.org/C84416704","wikidata":"https://www.wikidata.org/wiki/Q188666","display_name":"Adhesion","level":2,"score":0.4843836724758148},{"id":"https://openalex.org/C155619193","wikidata":"https://www.wikidata.org/wiki/Q415915","display_name":"Selectin","level":3,"score":0.43694767355918884},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.4273759424686432},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4214732348918915},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.34939444065093994},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3108954131603241},{"id":"https://openalex.org/C2776841590","wikidata":"https://www.wikidata.org/wiki/Q66589589","display_name":"Fucose","level":3,"score":0.2797623872756958},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.21343082189559937},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.17173907160758972},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.10893678665161133},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002240","descriptor_name":"Carbohydrate Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005732","descriptor_name":"Gangliosides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005732","descriptor_name":"Gangliosides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016256","descriptor_name":"Lewis X Antigen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018922","descriptor_name":"HL-60 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019040","descriptor_name":"E-Selectin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019040","descriptor_name":"E-Selectin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi971181t","doi":"https://doi.org/10.1021/bi971181t","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi971181t","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:9376344","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9376344","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"study_designs":[],"awards":[{"id":"https://openalex.org/G401348875","display_name":"GLYCOLIPIDS--CELL INTERACTION/PROLIFERATION/ONCOGENESIS","funder_award_id":"R35CA042505","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320338335","display_name":"H2020 European Research Council","ror":"https://ror.org/0472cxd90"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W229551611","https://openalex.org/W1516595608","https://openalex.org/W2043801746","https://openalex.org/W2117318310","https://openalex.org/W2155793158","https://openalex.org/W2265339669"],"related_works":["https://openalex.org/W1976340121","https://openalex.org/W2092190463","https://openalex.org/W2031775276","https://openalex.org/W2067488129","https://openalex.org/W2141369469","https://openalex.org/W1494017401","https://openalex.org/W2034185665","https://openalex.org/W181958975","https://openalex.org/W2515438736","https://openalex.org/W1973341387"],"abstract_inverted_index":{"The":[0],"majority":[1],"of":[2,35,40,48,56,68,76,117,127,237,259],"E-":[3],"and":[4,9,37,53,147,192,218,226,239],"P-selectin":[5],"ligands":[6,30],"in":[7,42,200,212,216],"leukocytes":[8,217,238],"myelocytic":[10],"or":[11,210,247],"monocytic":[12],"leukemia":[13],"cells":[14,83],"are":[15,31,207,232],"carried":[16],"by":[17],"transmembrane":[18],"glycoproteins":[19,43],"having":[20,133,150],"a":[21,73,134],"tandem":[22],"repeat":[23],"mucin-like":[24],"domain":[25],"through":[26,66],"which":[27],"O-linked":[28],"carbohydrate":[29],"carried.":[32],"However,":[33,203],"determination":[34],"structure":[36],"adhesive":[38],"function":[39],"carbohydrates":[41],"is":[44],"extremely":[45],"difficult":[46],"because":[47],"the":[49,54,121,144,158,233,253],"extensive":[50],"structural":[51],"heterogeneity":[52],"scarcity":[55],"material":[57],"for":[58,256],"functional":[59],"analysis.":[60],"We":[61,112],"have":[62],"overcome":[63],"this":[64],"difficulty":[65],"use":[67],"poly-LacNAc":[69,118,205],"gangliosides":[70,119,206,222],"isolated":[71],"from":[72,162,183,223],"large":[74],"quantity":[75],"(":[77],"approximately":[78],"1.2":[79],"L":[80],"packed)":[81],"HL60":[82,219,240],"[Stroud,":[84],"M.":[85,90],"R.,":[86],"Handa,":[87],"K.,":[88,92,94],"Salyan,":[89],"E.":[91],"Ito,":[93],"Levery,":[95],"S.":[96],"B.,":[97,102],"Hakomori,":[98],"S.,":[99],"Reinhold,":[100,104],"B.":[101],"&":[103],"V.":[105],"N.":[106],"(1996)":[107],"Biochemistry":[108],"35,":[109],"758-769,":[110],"770-778].":[111],"identified":[113],"two":[114],"major":[115,234],"types":[116],"without":[120],"sialosyl-Lex":[122,197],"epitope":[123,255],"as":[124,195],"being":[125],"capable":[126],"binding":[128,258],"to":[129,245,248],"E-selectin:":[130],"(i)":[131],"those":[132,149],"single":[135],"alpha1-->3":[136,152],"fucosylation":[137,153],"at":[138,143,154],"internal":[139,155],"GlcNAcs":[140],"but":[141,174],"not":[142,167],"penultimate":[145,159],"GlcNAc":[146],"(ii)":[148],"double":[151],"GlcNAcs,":[156],"excluding":[157],"GlcNAc.":[160],"Gangliosides":[161,182],"group":[163,184],"i":[164,225],"above":[165,186],"did":[166,196],"show":[168],"any":[169],"adhesion":[170,177,188],"under":[171,178,189,263],"static":[172,191],"conditions,":[173,194],"showed":[175,187],"strong":[176],"dynamic":[179,193,264],"flow":[180,265],"conditions.":[181,266],"ii":[185,227],"both":[190],"(SLex)-containing":[198],"structures":[199],"previous":[201],"studies.":[202],"SLex-containing":[204],"virtually":[208],"absent":[209],"present":[211],"only":[213],"trace":[214],"quantities":[215],"cells.":[220,241],"Poly-LacNAc":[221],"groups":[224],"above,":[228],"lacking":[229],"SLex":[230],"structure,":[231],"membrane":[235],"components":[236],"These":[242],"carbohydrates,":[243],"bound":[244],"lipid":[246],"protein,":[249],"may":[250],"therefore":[251],"be":[252],"physiological":[254],"E-selectin-dependent":[257],"these":[260],"cells,":[261],"particularly":[262]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}