{"id":"https://openalex.org/W2007775432","doi":"https://doi.org/10.1111/j.1530-0277.1993.tb00849.x","title":"Cloning and Expression of the Full\u2010Length cDNAS Encoding Human Liver Class 1 and Class 2 Aldehyde Dehydrogenase","display_name":"Cloning and Expression of the Full\u2010Length cDNAS Encoding Human Liver Class 1 and Class 2 Aldehyde Dehydrogenase","publication_year":1993,"publication_date":"1993-08-01","ids":{"openalex":"https://openalex.org/W2007775432","doi":"https://doi.org/10.1111/j.1530-0277.1993.tb00849.x","mag":"2007775432","pmid":"https://pubmed.ncbi.nlm.nih.gov/8214422"},"language":"en","primary_location":{"id":"doi:10.1111/j.1530-0277.1993.tb00849.x","doi":"https://doi.org/10.1111/j.1530-0277.1993.tb00849.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1530-0277.1993.tb00849.x","pdf_url":null,"source":{"id":"https://openalex.org/S115510430","display_name":"Alcoholism Clinical and Experimental Research","issn_l":"0145-6008","issn":["0145-6008","1530-0277","2993-7175"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Alcoholism: Clinical and Experimental Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101091861","display_name":"Chaofeng Zheng","orcid":"https://orcid.org/0009-0002-2203-2006","observed_orcids":["https://orcid.org/0009-0002-2203-2006"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chao\u2010Feng Zheng","raw_affiliation_strings":["Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907-1153"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907-1153","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086523704","display_name":"Thomas T. Y. Wang","orcid":"https://orcid.org/0000-0003-2299-9452","observed_orcids":["https://orcid.org/0000-0003-2299-9452"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas T. Y. Wang","raw_affiliation_strings":["Department of Biochemistry (C.-F.Z., H.W.), Purdue University, West Lafayette, Indiana: and Laboratory of Nutritional and Molecular Regulation (T.T.Y.W.), National Cancer Institute, Frederick, Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry (C.-F.Z., H.W.), Purdue University, West Lafayette, Indiana: and Laboratory of Nutritional and Molecular Regulation (T.T.Y.W.), National Cancer Institute, Frederick, Maryland","institution_ids":["https://openalex.org/I219193219","https://openalex.org/I4210140884"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012958448","display_name":"Henry Weiner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Henry Weiner","raw_affiliation_strings":["Department of Biochemistry (C.-F.Z., H.W.), Purdue University, West Lafayette, Indiana: and Laboratory of Nutritional and Molecular Regulation (T.T.Y.W.), National Cancer Institute, Frederick, Maryland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry (C.-F.Z., H.W.), Purdue University, West Lafayette, Indiana: and Laboratory of Nutritional and Molecular Regulation (T.T.Y.W.), National Cancer Institute, Frederick, Maryland","institution_ids":["https://openalex.org/I219193219","https://openalex.org/I4210140884"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5012958448"],"corresponding_institution_ids":["https://openalex.org/I219193219","https://openalex.org/I4210140884"],"apc_list":{"value":4110,"currency":"USD","value_usd":4110},"apc_paid":null,"fwci":3.1123,"has_fulltext":false,"cited_by_count":67,"citation_normalized_percentile":{"value":0.92433278,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"17","issue":"4","first_page":"828","last_page":"831"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11207","display_name":"Alcohol Consumption and Health Effects","score":0.9359999895095825,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11207","display_name":"Alcohol Consumption and Health Effects","score":0.9359999895095825,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12688","display_name":"Microbial metabolism and enzyme function","score":0.013299999758601189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.00930000003427267,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aldehyde-dehydrogenase","display_name":"aldehyde dehydrogenase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/aldh1","display_name":"ALDH1","score":0.8029999732971191},{"id":"https://openalex.org/keywords/aldh2","display_name":"ALDH2","score":0.9990000128746033},{"id":"https://openalex.org/keywords/human-liver","display_name":"human liver","score":0.6690000295639038},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.8529999852180481},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9480000138282776},{"id":"https://openalex.org/keywords/mitochondrial-isozyme","display_name":"mitochondrial isozyme","score":0.8949999809265137},{"id":"https://openalex.org/keywords/cytosolic-isozyme","display_name":"cytosolic isozyme","score":1.0}],"concepts":[{"id":"https://openalex.org/C119795356","wikidata":"https://www.wikidata.org/wiki/Q407801","display_name":"Isozyme","level":3,"score":0.7952762246131897},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6868317723274231},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6651930809020996},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6648646593093872},{"id":"https://openalex.org/C25747580","wikidata":"https://www.wikidata.org/wiki/Q423792","display_name":"Aldehyde dehydrogenase","level":3,"score":0.6267430186271667},{"id":"https://openalex.org/C3175096","wikidata":"https://www.wikidata.org/wiki/Q2640417","display_name":"ALDH2","level":4,"score":0.6197000741958618},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.5393694639205933},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.46844983100891113},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.43784910440444946},{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.42908450961112976},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4265247583389282},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4145844876766205},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.33485108613967896}],"mesh":[{"descriptor_ui":"D000444","descriptor_name":"Aldehyde Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000444","descriptor_name":"Aldehyde Dehydrogenase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008930","descriptor_name":"Mitochondria, Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1530-0277.1993.tb00849.x","doi":"https://doi.org/10.1111/j.1530-0277.1993.tb00849.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1530-0277.1993.tb00849.x","pdf_url":null,"source":{"id":"https://openalex.org/S115510430","display_name":"Alcoholism Clinical and Experimental Research","issn_l":"0145-6008","issn":["0145-6008","1530-0277","2993-7175"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Alcoholism: Clinical and Experimental Research","raw_type":"journal-article"},{"id":"pmid:8214422","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8214422","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Alcoholism, clinical and experimental research","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2297022949","display_name":null,"funder_award_id":"AA00038","funder_id":"https://openalex.org/F4320337330","funder_display_name":"National Institute on Alcohol Abuse and Alcoholism"},{"id":"https://openalex.org/G574410073","display_name":"CEREBRAL BLOOD FLOW AND ENERGY METABOLISM IN THE CAT","funder_award_id":"1z01aa000038-02","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337330","display_name":"National Institute on Alcohol Abuse and Alcoholism","ror":"https://ror.org/02jzrsm59"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W30611308","https://openalex.org/W207146392","https://openalex.org/W1573199302","https://openalex.org/W1589027452","https://openalex.org/W1965158345","https://openalex.org/W1965763707","https://openalex.org/W1968401756","https://openalex.org/W1972816445","https://openalex.org/W1978109629","https://openalex.org/W2001342878","https://openalex.org/W2004146008","https://openalex.org/W2021021514","https://openalex.org/W2034647911","https://openalex.org/W2035482274","https://openalex.org/W2044850091","https://openalex.org/W2069358454","https://openalex.org/W2075145643","https://openalex.org/W2075283798","https://openalex.org/W2075296185","https://openalex.org/W2080037227","https://openalex.org/W2081351575","https://openalex.org/W2085024565","https://openalex.org/W2138270253","https://openalex.org/W2404468360","https://openalex.org/W3168192185"],"related_works":["https://openalex.org/W2387738538","https://openalex.org/W129341367","https://openalex.org/W2001342878","https://openalex.org/W2309412613","https://openalex.org/W49869251","https://openalex.org/W1995500542","https://openalex.org/W2004146008","https://openalex.org/W2914460688","https://openalex.org/W2024822227","https://openalex.org/W1575005673"],"abstract_inverted_index":{"The":[0,79,93,127,157],"amino":[1],"acid":[2],"sequences":[3,16],"of":[4,17,62,68,74,101,107,113,154],"both":[5,39],"human":[6,43],"class":[7],"1":[8],"and":[9,14,33,54,109,139,149],"2":[10],"aldehyde":[11],"dehydrogenase":[12],"(ALDH)":[13],"the":[15,18,35,51,75,124,132,146,172],"genes":[19],"coding":[20],"for":[21,145],"them":[22],"are":[23],"known.":[24],"Based":[25],"on":[26],"this":[27],"sequence":[28],"data,":[29],"we":[30],"designed":[31],"primers":[32],"isolated":[34],"full-length":[36],"cDNAs":[37,47],"encoding":[38],"isozymes":[40],"from":[41,123],"a":[42,60,89,97,110,151],"liver":[44],"mRNA":[45],"pool.":[46],"were":[48,84,117],"subcloned":[49],"in":[50,56,118],"plasmid":[52],"pT7-7":[53],"expressed":[55,82,128,158],"Escherichia":[57],"coli":[58],"with":[59,121],"yield":[61],"approximately":[63],"3":[64],"mg":[65],"ALDH":[66],"protein/liter":[67],"cell":[69],"culture,":[70],"although":[71],"only":[72],"one-third":[73],"enzyme":[76,148],"was":[77],"soluble.":[78],"soluble":[80],"recombinantly":[81],"ALDHs":[83],"purified":[85],"to":[86],"homogeneity":[87],"using":[88],"hydroxyacetophenone-Sepharose":[90],"affinity":[91],"column.":[92],"mitochondrial":[94,159,169],"isozyme":[95,130,160,174],"had":[96,131,150],"subunit":[98,134],"molecular":[99,135],"weight":[100,136],"55":[102],"kDa,":[103],"an":[104],"isoelectric":[105],"point":[106],"4.9,":[108],"specific":[111,152],"activity":[112,153],"1.10":[114],"units/mg,":[115],"which":[116],"good":[119],"agreement":[120],"that":[122,143],"native":[125,147],"enzyme.":[126],"cytosolic":[129,173,183],"same":[133],"(55":[137],"kDa)":[138],"pI":[140],"(5.4)":[141],"as":[142],"reported":[144],"0.26":[155],"units/mg.":[156],"could":[161,175],"be":[162,176],"recognized":[163,177],"by":[164,178],"antibodies":[165],"raised":[166,180],"against":[167,181],"rat":[168],"ALDH,":[170],"whereas":[171],"antibody":[179],"horse":[182],"ALDH.":[184]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
