{"id":"https://openalex.org/W2007675461","doi":"https://doi.org/10.1021/bi010962y","title":"Studies of the Mechanism of Phenol Hydroxylase: Mutants Tyr289Phe, Asp54Asn, and Arg281Met","display_name":"Studies of the Mechanism of Phenol Hydroxylase: Mutants Tyr289Phe, Asp54Asn, and Arg281Met","publication_year":2001,"publication_date":"2001-09-22","ids":{"openalex":"https://openalex.org/W2007675461","doi":"https://doi.org/10.1021/bi010962y","mag":"2007675461","pmid":"https://pubmed.ncbi.nlm.nih.gov/11591156"},"language":"en","primary_location":{"id":"doi:10.1021/bi010962y","doi":"https://doi.org/10.1021/bi010962y","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi010962y","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Dong Xu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I4210114445","display_name":"Michigan Medicine","ror":"https://ror.org/01zcpa714","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210114445"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dong Xu","raw_affiliation_strings":["Department of Biological Chemistry, University of Michigan, Medical School, Ann Arbor, Michigan 48109-0606","Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, 48109-0606, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, University of Michigan, Medical School, Ann Arbor, Michigan 48109-0606","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, 48109-0606, USA","institution_ids":["https://openalex.org/I27837315","https://openalex.org/I4210114445"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023125790","display_name":"David P. Ballou","orcid":"https://orcid.org/0000-0002-3648-0355","observed_orcids":["https://orcid.org/0000-0002-3648-0355"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David P. Ballou","raw_affiliation_strings":["Department of Biological Chemistry, University of Michigan, Medical School, Ann Arbor, Michigan 48109-0606"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, University of Michigan, Medical School, Ann Arbor, Michigan 48109-0606","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079221179","display_name":"Vincent Massey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vincent Massey","raw_affiliation_strings":["Department of Biological Chemistry, University of Michigan, Medical School, Ann Arbor, Michigan 48109-0606"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Chemistry, University of Michigan, Medical School, Ann Arbor, Michigan 48109-0606","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5023125790"],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.3029,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.86230187,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"40","issue":"41","first_page":"12369","last_page":"12378"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.6553000211715698,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.6553000211715698,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.23100000619888306,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.07050000131130219,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phenol-hydroxylase","display_name":"phenol hydroxylase","score":0.9950000047683716},{"id":"https://openalex.org/keywords/flavoprotein","display_name":"flavoprotein","score":0.9210000038146973},{"id":"https://openalex.org/keywords/nadph-reduction","display_name":"NADPH reduction","score":0.675000011920929},{"id":"https://openalex.org/keywords/flavin-redox-potential","display_name":"flavin redox potential","score":0.7120000123977661},{"id":"https://openalex.org/keywords/active-site-residues","display_name":"active site residues","score":0.6819999814033508},{"id":"https://openalex.org/keywords/hydrogen-bonding","display_name":"hydrogen bonding","score":0.5680000185966492},{"id":"https://openalex.org/keywords/flavin-cofactor","display_name":"flavin cofactor","score":0.5419999957084656},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7260000109672546}],"concepts":[{"id":"https://openalex.org/C33093398","wikidata":"https://www.wikidata.org/wiki/Q174337","display_name":"Flavin group","level":3,"score":0.854891300201416},{"id":"https://openalex.org/C71995715","wikidata":"https://www.wikidata.org/wiki/Q78574842","display_name":"Flavoprotein","level":3,"score":0.8488290905952454},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.727959156036377},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.6836825013160706},{"id":"https://openalex.org/C2781109383","wikidata":"https://www.wikidata.org/wiki/Q898497","display_name":"Hydroxylation","level":3,"score":0.6541162133216858},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.6468437314033508},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.6166589856147766},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.6055095195770264},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5575794577598572},{"id":"https://openalex.org/C55904794","wikidata":"https://www.wikidata.org/wiki/Q82682","display_name":"Redox","level":2,"score":0.4925892949104309},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.4902520775794983},{"id":"https://openalex.org/C57600042","wikidata":"https://www.wikidata.org/wiki/Q227564","display_name":"Oxidative phosphorylation","level":2,"score":0.486784428358078},{"id":"https://openalex.org/C62300524","wikidata":"https://www.wikidata.org/wiki/Q7115638","display_name":"Oxidative enzyme","level":3,"score":0.48008304834365845},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.47884511947631836},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.4499465227127075},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3879566192626953},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.10842344164848328},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.060736387968063354}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014250","descriptor_name":"Trichosporon","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D014250","descriptor_name":"Trichosporon","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi010962y","doi":"https://doi.org/10.1021/bi010962y","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi010962y","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:11591156","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11591156","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.46000000834465027,"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2869093499","display_name":null,"funder_award_id":"GM11106","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G4327018926","display_name":null,"funder_award_id":"GM 20877","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W36288320","https://openalex.org/W1485336678","https://openalex.org/W1489973189","https://openalex.org/W1561118044","https://openalex.org/W1564858254","https://openalex.org/W1576778788","https://openalex.org/W1581938764","https://openalex.org/W1848681546","https://openalex.org/W1876994579","https://openalex.org/W1973009377","https://openalex.org/W2082053795","https://openalex.org/W2097321270","https://openalex.org/W2158272530","https://openalex.org/W2166702006","https://openalex.org/W2329700644","https://openalex.org/W2432517183"],"related_works":["https://openalex.org/W2800527079","https://openalex.org/W2035971017","https://openalex.org/W1564858254","https://openalex.org/W1989595915","https://openalex.org/W2088969712","https://openalex.org/W2010238323","https://openalex.org/W2020405924","https://openalex.org/W1989887031","https://openalex.org/W2052844412","https://openalex.org/W2258513826"],"abstract_inverted_index":{"Three":[0],"residues":[1,218,263],"in":[2,105,148,239,264],"the":[3,7,20,34,46,50,53,61,64,68,77,97,106,146,149,160,166,170,178,187,192,195,210,225,229,233,240,244,247,258,265],"active":[4,226],"site":[5,227],"of":[6,19,23,52,86,164,169,186,224,246,250,257,267],"flavoprotein":[8],"phenol":[9],"hydroxylase":[10],"(PHHY)":[11],"were":[12,269],"independently":[13],"changed":[14],"by":[15,28,92,157],"site-directed":[16],"mutagenesis.":[17],"One":[18],"mutant":[21,162,248],"forms":[22,249],"PHHY,":[24,165,251],"Tyr289Phe,":[25],"is":[26,33,58,66,81,88,101,173,212],"reduced":[27,156,188],"NADPH":[29],"much":[30],"slower":[31,175],"than":[32,45,176,184],"wild-type":[35,47,54,179],"enzyme,":[36,55],"although":[37],"it":[38,80,153,252],"has":[39,72],"a":[40,102,205],"slightly":[41],"higher":[42],"redox":[43],"potential":[44],"enzyme.":[48,180],"In":[49,243],"structure":[51],"residue":[56,104,206],"Tyr289":[57,100,142],"hydrogen-bonded":[59],"with":[60,76,194],"FAD":[62,147],"when":[63,79,203],"latter":[65],"at":[67],"\"out\"":[69],"position":[70],"but":[71],"no":[73],"direct":[74],"contact":[75],"flavin":[78,189,234],"\"in\".":[82],"The":[83],"oxidative":[84,171,241],"half-reaction":[85,172],"PHHY":[87,268],"not":[89],"significantly":[90,174],"affected":[91],"this":[93],"mutation,":[94],"contrary":[95],"to":[96,214,221,236],"concept":[98],"that":[99,255],"critical":[103],"hydroxylation":[107],"reaction":[108],"[Enroth,":[109],"C.,":[110],"Neujahr,":[111],"H.,":[112],"Schneider,":[113],"G.,":[114],"and":[115,132,209],"Lindqvist,":[116],"Y.":[117],"(1998)":[118],"Structure":[119],"6,":[120],"605-617;":[121],"Ridder,":[122],"L.,":[123],"Mullholland,":[124],"A.":[125],"J.,":[126],"Rietjens,":[127],"I.":[128],"M.":[129],"C.":[130],"M.,":[131],"Vervoort,":[133],"J.":[134,136],"(2000)":[135],"Am.":[137],"Chem.":[138],"Soc.":[139],"122,":[140],"8728-8738].":[141],"may":[143],"help":[144],"stabilize":[145],"out":[150],"conformation":[151],"where":[152],"can":[154],"be":[155,222],"NADPH.":[158],"For":[159],"Asp54Asn":[161,181],"form":[163],"initial":[167],"step":[168],"for":[177,190,232],"utilizes":[182],"less":[183],"20%":[185],"hydroxylating":[191],"substrate":[193],"remainder":[196],"forming":[197],"H(2)O(2).":[198],"Similar":[199],"changes":[200],"are":[201,219],"observed":[202],"Arg281,":[204],"between":[207],"Asp54":[208],"solvent,":[211],"mutated":[213],"Met.":[215],"These":[216],"two":[217],"suggested":[220],"part":[223],"environment":[228],"enzyme":[230],"provides":[231],"cofactor":[235],"function":[237],"optimally":[238],"half-reaction.":[242],"construction":[245],"was":[253],"determined":[254],"11":[256],"previously":[259],"reported":[260],"amino":[261],"acid":[262],"sequence":[266],"incorrect.":[270]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
