{"id":"https://openalex.org/W2007226552","doi":"https://doi.org/10.1101/pdb.prot5085","title":"Mnemiopsis leidyi Spawning and Embryo Collection","display_name":"Mnemiopsis leidyi Spawning and Embryo Collection","publication_year":2008,"publication_date":"2008-11-01","ids":{"openalex":"https://openalex.org/W2007226552","doi":"https://doi.org/10.1101/pdb.prot5085","mag":"2007226552","pmid":"https://pubmed.ncbi.nlm.nih.gov/21356725"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot5085","doi":"https://doi.org/10.1101/pdb.prot5085","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot5085","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Kevin Pang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I117965899","display_name":"University of Hawai\u02bbi at M\u0101noa","ror":"https://ror.org/01wspgy28","country_code":"US","type":"education","lineage":["https://openalex.org/I117965899"]},{"id":"https://openalex.org/I1331384533","display_name":"University of Hawaii System","ror":"https://ror.org/03tzaeb71","country_code":"US","type":"education","lineage":["https://openalex.org/I1331384533"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin Pang","raw_affiliation_strings":["Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086568453","display_name":"Mark Q. Martindale","orcid":"https://orcid.org/0000-0002-5805-5640","observed_orcids":["https://orcid.org/0000-0002-5805-5640"]},"institutions":[{"id":"https://openalex.org/I117965899","display_name":"University of Hawai\u02bbi at M\u0101noa","ror":"https://ror.org/01wspgy28","country_code":"US","type":"education","lineage":["https://openalex.org/I117965899"]},{"id":"https://openalex.org/I1331384533","display_name":"University of Hawaii System","ror":"https://ror.org/03tzaeb71","country_code":"US","type":"education","lineage":["https://openalex.org/I1331384533"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark Q. Martindale","raw_affiliation_strings":["Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2008","issue":"11","first_page":"pdb.prot5085","last_page":"pdb.prot5085"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12142","display_name":"Marine Invertebrate Physiology and Ecology","score":0.9585000276565552,"subfield":{"id":"https://openalex.org/subfields/1911","display_name":"Paleontology"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12142","display_name":"Marine Invertebrate Physiology and Ecology","score":0.9585000276565552,"subfield":{"id":"https://openalex.org/subfields/1911","display_name":"Paleontology"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.014000000432133675,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10765","display_name":"Marine Biology and Ecology Research","score":0.012000000104308128,"subfield":{"id":"https://openalex.org/subfields/1910","display_name":"Oceanography"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mnemiopsis-leidyi","display_name":"Mnemiopsis leidyi","score":1.0},{"id":"https://openalex.org/keywords/comb-jellies","display_name":"comb jellies","score":0.7900000214576721},{"id":"https://openalex.org/keywords/ctenophores","display_name":"ctenophores","score":0.9739999771118164},{"id":"https://openalex.org/keywords/spawning","display_name":"spawning","score":0.9039999842643738},{"id":"https://openalex.org/keywords/embryo-recovery","display_name":"embryo recovery","score":0.9610000252723694},{"id":"https://openalex.org/keywords/laboratory-rearing","display_name":"laboratory rearing","score":0.5619999766349792},{"id":"https://openalex.org/keywords/larval-development","display_name":"larval development","score":0.7070000171661377},{"id":"https://openalex.org/keywords/animal-evolution","display_name":"animal evolution","score":0.4519999921321869},{"id":"https://openalex.org/keywords/invasive-species","display_name":"invasive species","score":0.5799999833106995}],"concepts":[{"id":"https://openalex.org/C2777744765","wikidata":"https://www.wikidata.org/wiki/Q2734755","display_name":"Hatching","level":2,"score":0.7094476819038391},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6819003224372864},{"id":"https://openalex.org/C67283656","wikidata":"https://www.wikidata.org/wiki/Q2587899","display_name":"Fecundity","level":3,"score":0.6674991250038147},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.5111814141273499},{"id":"https://openalex.org/C2779027399","wikidata":"https://www.wikidata.org/wiki/Q204170","display_name":"Darkness","level":2,"score":0.5005543231964111},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.48294728994369507},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.461318701505661},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.3990418314933777},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.11712637543678284},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.11108431220054626},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot5085","doi":"https://doi.org/10.1101/pdb.prot5085","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot5085","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:21356725","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21356725","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"CSH protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":1.0,"id":"https://metadata.un.org/sdg/14","display_name":"Life below water"}],"sustainable_development_goals_aurora":[{"score":0.8899999856948853,"id":"https://metadata.un.org/sdg/14","display_name":"Life below water"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W2151291338"],"related_works":["https://openalex.org/W2274270494","https://openalex.org/W1994226804","https://openalex.org/W4214620973","https://openalex.org/W4407301986","https://openalex.org/W7221543097","https://openalex.org/W4224952105","https://openalex.org/W3206586920","https://openalex.org/W2610359653","https://openalex.org/W7095963269","https://openalex.org/W1980483688"],"abstract_inverted_index":{"INTRODUCTIONCtenophores,":[0],"or":[1],"comb":[2],"jellies,":[3],"are":[4,60,92,131],"a":[5,19],"group":[6],"of":[7,27,62,67,136,145,161,189],"marine":[8],"animals":[9,148],"whose":[10],"unique":[11],"biological":[12],"features":[13],"and":[14,36,69,95],"phylogenetic":[15],"placement":[16],"make":[17],"them":[18],"key":[20],"taxon":[21],"for":[22,180],"understanding":[23],"animal":[24,169],"evolution.":[25],"Because":[26,138],"its":[28],"large":[29,83],"size,":[30],"fecundity,":[31],"abundance":[32],"in":[33,82,105,154],"coastal":[34],"areas,":[35],"recent":[37],"introduction":[38],"to":[39,73,112,167,187],"European":[40],"waters,":[41],"Mnemiopsis":[42],"leidyi":[43],"(commonly":[44],"called":[45],"\"sea":[46],"walnuts\")":[47],"is":[48,140,165,191],"the":[49,106,134,143,155,159],"most":[50],"highly":[51],"studied":[52],"ctenophore.":[53],"Under":[54,118],"optimal":[55],"conditions,":[56,120],"these":[57],"self-fertile":[58],"hermaphrodites":[59],"capable":[61],"reproduction":[63],"at":[64,171],"2":[65],"wk":[66],"age":[68],"can":[70,79,96,102,157,177],"release":[71],"up":[72],"10,000":[74],"eggs":[75,130],"per":[76],"day.":[77],"Adults":[78],"be":[80,97,103,178],"maintained":[81],"aquaria":[84],"with":[85],"gentle":[86],"aeration":[87],"as":[88,90],"long":[89],"they":[91],"well":[93],"fed":[94],"spawned":[98],"daily;":[99],"multiple":[100],"generations":[101],"raised":[104],"laboratory.":[107],"This":[108,163],"protocol":[109,164],"describes":[110],"how":[111],"collect":[113],"embryos":[114],"from":[115,185],"M.":[116],"leidyi.":[117],"natural":[119],"spawning":[121,139,170,186],"normally":[122],"occurs":[123],"~8":[124],"h":[125],"after":[126],"sunset,":[127],"such":[128],"that":[129],"released":[132],"under":[133,149],"cover":[135],"darkness.":[137],"triggered":[141],"by":[142],"onset":[144],"darkness,":[146],"keeping":[147],"an":[150],"artificial":[151],"light":[152],"regimen":[153],"laboratory":[156],"alter":[158],"time":[160],"spawning.":[162],"designed":[166],"induce":[168],"approximately":[172],"11:00":[173],"a.m.;":[174],"however,":[175],"it":[176],"adjusted":[179],"other":[181],"times.":[182],"The":[183],"duration":[184],"hatching":[188],"larvae":[190],"18-24":[192],"h.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":9},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}