{"id":"https://openalex.org/W2007128052","doi":"https://doi.org/10.1016/j.jviromet.2014.05.004","title":"An improved method for simple and efficient hepatitis B virus genome cloning","display_name":"An improved method for simple and efficient hepatitis B virus genome cloning","publication_year":2014,"publication_date":"2014-05-14","ids":{"openalex":"https://openalex.org/W2007128052","doi":"https://doi.org/10.1016/j.jviromet.2014.05.004","mag":"2007128052","pmid":"https://pubmed.ncbi.nlm.nih.gov/24833122"},"language":"en","primary_location":{"id":"doi:10.1016/j.jviromet.2014.05.004","doi":"https://doi.org/10.1016/j.jviromet.2014.05.004","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2014.05.004","pdf_url":null,"source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026622757","display_name":"Zeng Tu","orcid":"https://orcid.org/0000-0002-0329-4097","observed_orcids":["https://orcid.org/0000-0002-0329-4097"]},"institutions":[{"id":"https://openalex.org/I170203145","display_name":"MRC Laboratory of Molecular Biology","ror":"https://ror.org/00tw3jy02","country_code":"GB","type":"facility","lineage":["https://openalex.org/I170203145","https://openalex.org/I4210087105","https://openalex.org/I90344618"]},{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN","GB"],"is_corresponding":false,"raw_author_name":"Zeng Tu","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China","MRC Laboratory of Molecular Biology, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]},{"raw_affiliation_string":"MRC Laboratory of Molecular Biology, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I170203145"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009921555","display_name":"Yuan Hu","orcid":"https://orcid.org/0000-0002-0038-1574","observed_orcids":["https://orcid.org/0000-0002-0038-1574"]},"institutions":[{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuan Hu","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101759386","display_name":"Lei Xu","orcid":"https://orcid.org/0000-0002-9886-3677","observed_orcids":["https://orcid.org/0000-0002-9886-3677"]},"institutions":[{"id":"https://openalex.org/I170203145","display_name":"MRC Laboratory of Molecular Biology","ror":"https://ror.org/00tw3jy02","country_code":"GB","type":"facility","lineage":["https://openalex.org/I170203145","https://openalex.org/I4210087105","https://openalex.org/I90344618"]},{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN","GB"],"is_corresponding":false,"raw_author_name":"Lei Xu","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China","MRC Laboratory of Molecular Biology, Cambridge, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]},{"raw_affiliation_string":"MRC Laboratory of Molecular Biology, Cambridge, United Kingdom","institution_ids":["https://openalex.org/I170203145"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101482500","display_name":"Yong Huang","orcid":"https://orcid.org/0000-0002-7711-3770","observed_orcids":["https://orcid.org/0000-0002-7711-3770"]},"institutions":[{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yong Huang","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059375173","display_name":"Xuan Luo","orcid":"https://orcid.org/0000-0002-9579-133X","observed_orcids":["https://orcid.org/0000-0002-9579-133X"]},"institutions":[{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xuan Luo","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073791602","display_name":"Jieli Hu","orcid":"https://orcid.org/0000-0002-9869-659X","observed_orcids":["https://orcid.org/0000-0002-9869-659X"]},"institutions":[{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jie-li Hu","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Electronic address: hujieli1977@163.com","Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Electronic address: hujieli1977@163.com","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]},{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029583137","display_name":"Ailong Huang","orcid":"https://orcid.org/0000-0003-0148-7423","observed_orcids":["https://orcid.org/0000-0003-0148-7423"]},"institutions":[{"id":"https://openalex.org/I4210097509","display_name":"Second Affiliated Hospital of Chongqing Medical University","ror":"https://ror.org/00r67fz39","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210097509"]},{"id":"https://openalex.org/I87780372","display_name":"Chongqing Medical University","ror":"https://ror.org/017z00e58","country_code":"CN","type":"education","lineage":["https://openalex.org/I87780372"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ai-long Huang","raw_affiliation_strings":["Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Electronic address: ahuang1964@163.com","Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. Electronic address: ahuang1964@163.com","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]},{"raw_affiliation_string":"Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China","institution_ids":["https://openalex.org/I4210097509","https://openalex.org/I87780372"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3210,"currency":"USD","value_usd":3210},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"205","issue":null,"first_page":"75","last_page":"80"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10151","display_name":"Hepatitis C virus research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatitis-b-virus","display_name":"hepatitis B virus","score":1.0},{"id":"https://openalex.org/keywords/lacz","display_name":"lacZ","score":0.5400000214576721},{"id":"https://openalex.org/keywords/blue-white-screening","display_name":"blue-white screening","score":0.9950000047683716},{"id":"https://openalex.org/keywords/serum-samples","display_name":"serum samples","score":0.4300000071525574}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7967096567153931},{"id":"https://openalex.org/C2780593183","wikidata":"https://www.wikidata.org/wiki/Q6844","display_name":"Hepatitis B virus","level":3,"score":0.7398192882537842},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6556360125541687},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6054193377494812},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.5396080613136292},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5163711309432983},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5058633685112},{"id":"https://openalex.org/C58301748","wikidata":"https://www.wikidata.org/wiki/Q1074969","display_name":"Restriction site","level":4,"score":0.43607857823371887},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.4179420471191406},{"id":"https://openalex.org/C2889275","wikidata":"https://www.wikidata.org/wiki/Q7316313","display_name":"Restriction digest","level":4,"score":0.41469770669937134},{"id":"https://openalex.org/C189819185","wikidata":"https://www.wikidata.org/wiki/Q384283","display_name":"Subgenomic mRNA","level":4,"score":0.4104308485984802},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.38968533277511597},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.354422390460968},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3322391211986542},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006509","descriptor_name":"Hepatitis B","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":true},{"descriptor_ui":"D006515","descriptor_name":"Hepatitis B virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014644","descriptor_name":"Genetic Variation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016679","descriptor_name":"Genome, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jviromet.2014.05.004","doi":"https://doi.org/10.1016/j.jviromet.2014.05.004","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2014.05.004","pdf_url":null,"source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},{"id":"pmid:24833122","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24833122","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1521693111","https://openalex.org/W1969524951","https://openalex.org/W1994936808","https://openalex.org/W2029003567","https://openalex.org/W2064629173","https://openalex.org/W2107683172","https://openalex.org/W2111152683","https://openalex.org/W2122158190","https://openalex.org/W2138240824","https://openalex.org/W2139053886","https://openalex.org/W2140082212","https://openalex.org/W2143501119","https://openalex.org/W2148399664","https://openalex.org/W2156552801","https://openalex.org/W2463256028"],"related_works":["https://openalex.org/W2358969467","https://openalex.org/W2375208414","https://openalex.org/W2375671623","https://openalex.org/W4327920391","https://openalex.org/W2405965976","https://openalex.org/W2380592694","https://openalex.org/W2388926560","https://openalex.org/W2357384767","https://openalex.org/W1992547371","https://openalex.org/W2071480332"],"abstract_inverted_index":null,"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
