{"id":"https://openalex.org/W2006785647","doi":"https://doi.org/10.1074/jbc.275.9.6055","title":"Phosphorylation of a Src Kinase at the Autophosphorylation Site in the Absence of Src Kinase Activity","display_name":"Phosphorylation of a Src Kinase at the Autophosphorylation Site in the Absence of Src Kinase Activity","publication_year":2000,"publication_date":"2000-03-01","ids":{"openalex":"https://openalex.org/W2006785647","doi":"https://doi.org/10.1074/jbc.275.9.6055","mag":"2006785647","pmid":"https://pubmed.ncbi.nlm.nih.gov/10692391"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.275.9.6055","doi":"https://doi.org/10.1074/jbc.275.9.6055","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.275.9.6055","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.275.9.6055","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071873704","display_name":"Gary Gene Chiang","orcid":"https://orcid.org/0000-0003-3004-7400","observed_orcids":["https://orcid.org/0000-0003-3004-7400"]},"institutions":[{"id":"https://openalex.org/I1307098950","display_name":"Salk Institute for Biological Studies","ror":"https://ror.org/03xez1567","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1307098950"]},{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Gary G. Chiang","raw_affiliation_strings":["Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA. gchiang@ucsd.edu","Department of Biology, University of California, San Diego, La Jolla, California 92093","Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA. gchiang@ucsd.edu","institution_ids":["https://openalex.org/I1307098950"]},{"raw_affiliation_string":"Department of Biology, University of California, San Diego, La Jolla, California 92093","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037","institution_ids":["https://openalex.org/I1307098950"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053274271","display_name":"Bartholomew M. Sefton","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1307098950","display_name":"Salk Institute for Biological Studies","ror":"https://ror.org/03xez1567","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1307098950"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bartholomew M. Sefton","raw_affiliation_strings":["Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037","#N#\u2020Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037","institution_ids":["https://openalex.org/I1307098950"]},{"raw_affiliation_string":"#N#\u2020Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037","institution_ids":["https://openalex.org/I1307098950"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5071873704"],"corresponding_institution_ids":["https://openalex.org/I1307098950","https://openalex.org/I36258959"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.9954,"has_fulltext":false,"cited_by_count":38,"citation_normalized_percentile":{"value":0.71859718,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"275","issue":"9","first_page":"6055","last_page":"6058"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.023600000888109207,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.019200000911951065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lck","display_name":"Lck","score":0.8349999785423279},{"id":"https://openalex.org/keywords/src-family-kinases","display_name":"Src family kinases","score":0.9300000071525574},{"id":"https://openalex.org/keywords/autophosphorylation","display_name":"autophosphorylation","score":0.7919999957084656},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.9459999799728394},{"id":"https://openalex.org/keywords/oxidant-signaling","display_name":"oxidant signaling","score":0.6880000233650208},{"id":"https://openalex.org/keywords/fibroblasts","display_name":"fibroblasts","score":0.8040000200271606},{"id":"https://openalex.org/keywords/tyrosine-kinase","display_name":"tyrosine kinase","score":0.7269999980926514},{"id":"https://openalex.org/keywords/kinase-activators","display_name":"kinase activators","score":0.49900001287460327}],"concepts":[{"id":"https://openalex.org/C150109051","wikidata":"https://www.wikidata.org/wiki/Q3630494","display_name":"Autophosphorylation","level":4,"score":0.9027079343795776},{"id":"https://openalex.org/C108636557","wikidata":"https://www.wikidata.org/wiki/Q21109354","display_name":"Proto-oncogene tyrosine-protein kinase Src","level":3,"score":0.8532546162605286},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.4547116756439209},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4273059368133545},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36363959312438965},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3324577212333679},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.23714420199394226},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22291168570518494}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019000","descriptor_name":"Phosphotyrosine","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D019061","descriptor_name":"src-Family Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019860","descriptor_name":"Lymphocyte Specific Protein Tyrosine Kinase p56(lck)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.275.9.6055","doi":"https://doi.org/10.1074/jbc.275.9.6055","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.275.9.6055","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:10692391","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10692391","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.275.9.6055","doi":"https://doi.org/10.1074/jbc.275.9.6055","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.275.9.6055","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1677931696","display_name":"CANCER RESEARCH IN MEDICAL RADIATION PHYSICS","funder_award_id":"T32CA009435","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6603634540","display_name":"THYMOMA TYROSINE PROTEIN KINASE","funder_award_id":"R01CA042350","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G9000880274","display_name":"Cancer Center Support Grant (CCSG)","funder_award_id":"P30CA014195","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":54,"referenced_works":["https://openalex.org/W87002874","https://openalex.org/W199729741","https://openalex.org/W207547976","https://openalex.org/W1489777708","https://openalex.org/W1524455540","https://openalex.org/W1562110281","https://openalex.org/W1582379379","https://openalex.org/W1867931780","https://openalex.org/W1943505406","https://openalex.org/W1967448808","https://openalex.org/W1979034912","https://openalex.org/W1991683855","https://openalex.org/W2008799785","https://openalex.org/W2011147958","https://openalex.org/W2012681367","https://openalex.org/W2016178838","https://openalex.org/W2016553217","https://openalex.org/W2028669354","https://openalex.org/W2032582358","https://openalex.org/W2033444434","https://openalex.org/W2034152669","https://openalex.org/W2035562653","https://openalex.org/W2040328392","https://openalex.org/W2048569323","https://openalex.org/W2050465795","https://openalex.org/W2052092007","https://openalex.org/W2053383702","https://openalex.org/W2058127710","https://openalex.org/W2061152334","https://openalex.org/W2063443629","https://openalex.org/W2065289702","https://openalex.org/W2068502127","https://openalex.org/W2070316997","https://openalex.org/W2075767774","https://openalex.org/W2079123009","https://openalex.org/W2079896268","https://openalex.org/W2080401826","https://openalex.org/W2084434864","https://openalex.org/W2085664820","https://openalex.org/W2088215519","https://openalex.org/W2094173028","https://openalex.org/W2101257013","https://openalex.org/W2109202813","https://openalex.org/W2124132224","https://openalex.org/W2129742695","https://openalex.org/W2140490890","https://openalex.org/W2146157811","https://openalex.org/W2146578030","https://openalex.org/W2146989813","https://openalex.org/W2156320225","https://openalex.org/W2162309468","https://openalex.org/W2165052240","https://openalex.org/W2398661146","https://openalex.org/W2471808755"],"related_works":["https://openalex.org/W2156320225","https://openalex.org/W1971058672","https://openalex.org/W1984521167","https://openalex.org/W2075438429","https://openalex.org/W1667965282","https://openalex.org/W1990435763","https://openalex.org/W375261788","https://openalex.org/W1970722898","https://openalex.org/W2460715479","https://openalex.org/W2308778564"],"abstract_inverted_index":{"Exposure":[0],"of":[1,8,25,75,93,122,125,135,144,168,174,184,188],"cells":[2,123,137,180],"to":[3,44,52,87,131,141,146,150],"oxidants":[4],"increases":[5],"the":[6,9,22,26,91,142,182],"phosphorylation":[7,92,143,167,173],"Src":[10,82,100,127,159,189],"family":[11,83,101,128,160,190],"tyrosine":[12,107],"protein":[13,108],"kinase":[14,68,102,129],"Lck":[15,30,40,71,117,175],"at":[16,47,176],"Tyr-394,":[17],"a":[18,67],"conserved":[19],"residue":[20],"in":[21,32,115,178],"activation":[23],"loop":[24],"catalytic":[27],"domain.":[28],"Kinase-deficient":[29],"expressed":[31],"fibroblasts":[33,79],"that":[34,57,66,151,158],"do":[35],"not":[36,163],"express":[37,80],"any":[38],"endogenous":[39,126],"has":[41],"been":[42],"shown":[43],"be":[45,88],"phosphorylated":[46,119],"Tyr-394":[48,94,114,145,177],"following":[49,120],"H(2)O(2)":[50,139],"treatment":[51,134],"an":[53,105,147,185],"extent":[54,148],"indistinguishable":[55],"from":[56],"seen":[58,152],"with":[59,138,153],"wild":[60,154],"type":[61,155],"Lck.":[62,169],"This":[63],"finding":[64],"indicates":[65],"other":[69,81],"than":[70],"itself":[72],"is":[73],"capable":[74],"phosphorylating":[76],"Tyr-394.":[77],"Because":[78],"members,":[84],"it":[85],"remained":[86],"determined":[89],"whether":[90,113],"was":[95,118],"carried":[96],"out":[97],"by":[98,104],"another":[99],"or":[103],"unrelated":[106],"kinase.":[109],"We":[110],"examined":[111],"here":[112],"kinase-deficient":[116],"exposure":[121],"devoid":[124],"activity":[130],"H(2)O(2).":[132],"Strikingly,":[133],"such":[136],"led":[140],"identical":[149],"Lck,":[156],"demonstrating":[157],"kinases":[161],"are":[162],"required":[164],"for":[165],"H(2)O(2)-induced":[166],"Furthermore,":[170],"this":[171],"efficient":[172],"non-lymphoid":[179],"suggests":[181],"existence":[183],"ubiquitous":[186],"activator":[187],"kinases.":[191]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}