{"id":"https://openalex.org/W2006144008","doi":"https://doi.org/10.1002/pro.5560050810","title":"Crystal structures of the active site mutant (Arg\u2010243 \u2192 Ala) in the T and R allosteric states of pig kidney fructose\u20101,6\u2010bisphosphatase expressed in escherichia coli","display_name":"Crystal structures of the active site mutant (Arg\u2010243 \u2192 Ala) in the T and R allosteric states of pig kidney fructose\u20101,6\u2010bisphosphatase expressed in escherichia coli","publication_year":1996,"publication_date":"1996-08-01","ids":{"openalex":"https://openalex.org/W2006144008","doi":"https://doi.org/10.1002/pro.5560050810","mag":"2006144008","pmid":"https://pubmed.ncbi.nlm.nih.gov/8844845","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2143480"},"language":"en","primary_location":{"id":"doi:10.1002/pro.5560050810","doi":"https://doi.org/10.1002/pro.5560050810","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pro.5560050810","pdf_url":null,"source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2143480","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084942532","display_name":"Boguslaw Stec","orcid":"https://orcid.org/0000-0002-8696-0349","observed_orcids":["https://orcid.org/0000-0002-8696-0349"]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Boguslaw Stec","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167, USA","Department of Chemistry, Merkert Chemistry Center Boston College, Chestnut Hill, Massachusetts 02167"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167, USA","institution_ids":["https://openalex.org/I103531236"]},{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center Boston College, Chestnut Hill, Massachusetts 02167","institution_ids":["https://openalex.org/I103531236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112471326","display_name":"Reimar Abraham","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Reimar Abraham","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167","institution_ids":["https://openalex.org/I103531236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009139107","display_name":"Eug\u00e8ne L. Giroux","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eugene Giroux","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167","institution_ids":["https://openalex.org/I103531236"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5105414035","display_name":"Evan R. Kantrowitz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Evan R. Kantrowitz","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167","institution_ids":["https://openalex.org/I103531236"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5105414035"],"corresponding_institution_ids":["https://openalex.org/I103531236"],"apc_list":{"value":4480,"currency":"USD","value_usd":4480},"apc_paid":null,"fwci":0.6744,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.66559074,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"5","issue":"8","first_page":"1541","last_page":"1553"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5859000086784363,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.5859000086784363,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.3813000023365021,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.01979999989271164,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fructose-1-6-bisphosphatase","display_name":"fructose-1,6-bisphosphatase","score":1.0},{"id":"https://openalex.org/keywords/active-site-mutants","display_name":"active site mutants","score":0.6890000104904175},{"id":"https://openalex.org/keywords/allosteric-regulation","display_name":"allosteric regulation","score":0.8100000023841858},{"id":"https://openalex.org/keywords/fructose-2-6-bisphosphate","display_name":"fructose-2,6-bisphosphate","score":0.9010000228881836},{"id":"https://openalex.org/keywords/fructose-1-6-bisphosphate","display_name":"fructose-1,6-bisphosphate","score":0.9330000281333923},{"id":"https://openalex.org/keywords/amp-inhibition","display_name":"AMP inhibition","score":0.925000011920929},{"id":"https://openalex.org/keywords/magnesium-binding","display_name":"magnesium binding","score":0.9229999780654907},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.6970000267028809},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5289999842643738}],"concepts":[{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.9178151488304138},{"id":"https://openalex.org/C189909181","wikidata":"https://www.wikidata.org/wiki/Q899507","display_name":"Fructose 1,6-bisphosphatase","level":3,"score":0.8917508721351624},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.8305014967918396},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.637234091758728},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.5684583783149719},{"id":"https://openalex.org/C2776970464","wikidata":"https://www.wikidata.org/wiki/Q122043","display_name":"Fructose","level":2,"score":0.5672982931137085},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.566405713558197},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5440948605537415},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.46907705068588257},{"id":"https://openalex.org/C2777339483","wikidata":"https://www.wikidata.org/wiki/Q647203","display_name":"Conformational change","level":2,"score":0.4108280837535858},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38270679116249084},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000494","descriptor_name":"Allosteric Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001120","descriptor_name":"Arginine","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004563","descriptor_name":"Electrochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006597","descriptor_name":"Fructose-Bisphosphatase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D006597","descriptor_name":"Fructose-Bisphosphatase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006597","descriptor_name":"Fructose-Bisphosphatase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006597","descriptor_name":"Fructose-Bisphosphatase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006600","descriptor_name":"Hexosephosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006600","descriptor_name":"Hexosephosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/pro.5560050810","doi":"https://doi.org/10.1002/pro.5560050810","is_oa":false,"landing_page_url":"https://doi.org/10.1002/pro.5560050810","pdf_url":null,"source":{"id":"https://openalex.org/S156919612","display_name":"Protein Science","issn_l":"0961-8368","issn":["0961-8368","1469-896X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Science","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2143480","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2143480","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Sci","raw_type":"Text"},{"id":"pmid:8844845","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8844845","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein science : a publication of the Protein Society","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2143480","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2143480","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Sci","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320308357","display_name":"Boston College","ror":"https://ror.org/02n2fzt79"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1437056","https://openalex.org/W1488576198","https://openalex.org/W1526007035","https://openalex.org/W1527664600","https://openalex.org/W1529429892","https://openalex.org/W1569135515","https://openalex.org/W1580989767","https://openalex.org/W1584476217","https://openalex.org/W1650316802","https://openalex.org/W1966041739","https://openalex.org/W1970185106","https://openalex.org/W1979234818","https://openalex.org/W1982461926","https://openalex.org/W1982636049","https://openalex.org/W1984669641","https://openalex.org/W1986191025","https://openalex.org/W1995822310","https://openalex.org/W1998591953","https://openalex.org/W2010439656","https://openalex.org/W2011704285","https://openalex.org/W2041827468","https://openalex.org/W2042987185","https://openalex.org/W2044666081","https://openalex.org/W2045275234","https://openalex.org/W2048741442","https://openalex.org/W2050771330","https://openalex.org/W2056984665","https://openalex.org/W2060503091","https://openalex.org/W2069536318","https://openalex.org/W2088363148","https://openalex.org/W2089543391","https://openalex.org/W2094120471","https://openalex.org/W2116887683","https://openalex.org/W2128421575","https://openalex.org/W2146962215","https://openalex.org/W2398388055"],"related_works":["https://openalex.org/W1529429892","https://openalex.org/W2056984665","https://openalex.org/W1970111310","https://openalex.org/W1569135515","https://openalex.org/W2159870370","https://openalex.org/W2066164089","https://openalex.org/W2084883939","https://openalex.org/W2099936813","https://openalex.org/W2102923303","https://openalex.org/W1972682439"],"abstract_inverted_index":{"The":[0,102,171],"active":[1,24,114,162],"site":[2,25,115,163],"of":[3,14,26,38,42,60,112,125,134,138,152,160,168,176,184,189,196,204],"pig":[4],"kidney":[5],"fructose-1,6-bisphosphatase":[6],"(EC":[7],"3.1.3.11)":[8],"is":[9,210],"shared":[10],"between":[11],"subunits,":[12],"Arg-243":[13,46,185,205],"one":[15],"chain":[16],"interacting":[17],"with":[18,45,117,165],"fructose-1,6-bisphosphate":[19,78],"or":[20],"fructose-2,6-bisphosphate":[21,87],"in":[22,51,109,149,173,186,206,213],"the":[23,35,39,43,61,65,76,85,110,113,121,139,150,153,161,166,169,177,181,187,193,197,202,207],"an":[27],"adjacent":[28],"chain.":[29],"In":[30],"this":[31],"study,":[32],"we":[33],"present":[34],"X-ray":[36,103,194],"structures":[37,104,122,142,195],"mutant":[40,178],"version":[41],"enzyme":[44,63,179,199,209],"replaced":[47],"by":[48,70],"alanine,":[49],"crystallized":[50],"both":[52],"T":[53,140],"and":[54,68,82,120,131,157],"R":[55],"allosteric":[56],"states.":[57],"Kinetic":[58],"characteristics":[59],"altered":[62],"showed":[64],"magnesium":[66],"binding":[67],"inhibition":[69],"AMP":[71,155],"differed":[72],"slightly;":[73],"affinity":[74,83],"for":[75,84],"substrate":[77],"was":[79,88],"reduced":[80,89],"10-fold":[81],"inhibitor":[86],"1,000-fold":[90],"(Giroux":[91],"E,":[92],"Williams":[93],"MK,":[94],"Kantrowitz":[95],"ER,":[96],"1994,":[97],"J":[98],"Biol":[99],"Chem":[100],"269:31404-31409).":[101],"show":[105],"no":[106],"major":[107],"changes":[108,148],"organization":[111],"compared":[116],"wild-type":[118,208],"enzyme,":[119],"confirm":[123],"predictions":[124],"molecular":[126],"dynamics":[127],"simulations":[128],"involving":[129],"Lys-269":[130],"Lys-274.":[132],"Comparison":[133],"two":[135],"independent":[136],"models":[137],"form":[141],"have":[143],"revealed":[144],"small":[145,158],"but":[146],"significant":[147],"conformation":[151],"bound":[154],"molecules":[156],"reorganization":[159],"correlated":[164],"presence":[167],"inhibitor.":[170],"differences":[172],"kinetic":[174],"properties":[175],"indicate":[180],"key":[182],"importance":[183],"function":[188],"fructose-1,6-bisphosphatase.":[190],"Calculations":[191],"using":[192],"Arg-243-->Ala":[198],"suggest":[200],"that":[201],"role":[203],"predominantly":[211],"electrostatic":[212],"nature.":[214]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
