{"id":"https://openalex.org/W2006124126","doi":"https://doi.org/10.1016/0003-2697(88)90047-4","title":"A simple method for DNA purification from peripheral blood","display_name":"A simple method for DNA purification from peripheral blood","publication_year":1988,"publication_date":"1988-11-01","ids":{"openalex":"https://openalex.org/W2006124126","doi":"https://doi.org/10.1016/0003-2697(88)90047-4","mag":"2006124126","pmid":"https://pubmed.ncbi.nlm.nih.gov/3239751"},"language":"en","primary_location":{"id":"doi:10.1016/0003-2697(88)90047-4","doi":"https://doi.org/10.1016/0003-2697(88)90047-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(88)90047-4","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036246396","display_name":"Thomas A. Ciulla","orcid":"https://orcid.org/0000-0001-5557-6777","observed_orcids":["https://orcid.org/0000-0001-5557-6777"]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas A. Ciulla","raw_affiliation_strings":["Neuroimmunology Unit, Massachusetts General Hospital, Boston 02114"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Neuroimmunology Unit, Massachusetts General Hospital, Boston 02114","institution_ids":["https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052480714","display_name":"R. Sklar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert M. Sklar","raw_affiliation_strings":["Day Neuromuscular Research Center, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts 02114 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Day Neuromuscular Research Center, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts 02114 USA","institution_ids":["https://openalex.org/I4210087915"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5104805489","display_name":"Stephen L. Hauser","orcid":"https://orcid.org/0000-0002-4932-4001","observed_orcids":["https://orcid.org/0000-0002-4932-4001"]},"institutions":[{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Stephen L. Hauser","raw_affiliation_strings":["Neuroimmunology Unit, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts 02114 USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Neuroimmunology Unit, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts 02114 USA","institution_ids":["https://openalex.org/I4210087915"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5104805489"],"corresponding_institution_ids":["https://openalex.org/I4210087915"],"apc_list":{"value":3540,"currency":"USD","value_usd":3540},"apc_paid":null,"fwci":0.8958,"has_fulltext":false,"cited_by_count":229,"citation_normalized_percentile":{"value":0.72301742,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"174","issue":"2","first_page":"485","last_page":"488"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10261","display_name":"Genetic Associations and Epidemiology","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10751","display_name":"Forensic and Genetic Research","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-extraction","display_name":"DNA extraction","score":0.7409999966621399},{"id":"https://openalex.org/keywords/peripheral-blood","display_name":"peripheral blood","score":0.9480000138282776},{"id":"https://openalex.org/keywords/genomic-dna","display_name":"genomic DNA","score":0.5419999957084656},{"id":"https://openalex.org/keywords/guanidine-isothiocyanate","display_name":"guanidine isothiocyanate","score":0.9369999766349792},{"id":"https://openalex.org/keywords/isopropanol-precipitation","display_name":"isopropanol precipitation","score":0.9160000085830688},{"id":"https://openalex.org/keywords/southern-blot","display_name":"Southern blot","score":0.7429999709129333}],"concepts":[{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.7495912909507751},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7250089049339294},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.6708132028579712},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5513060092926025},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.47663864493370056},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4609456956386566},{"id":"https://openalex.org/C2776572452","wikidata":"https://www.wikidata.org/wiki/Q416049","display_name":"Isothiocyanate","level":2,"score":0.43910273909568787},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.4383261203765869},{"id":"https://openalex.org/C3017910909","wikidata":"https://www.wikidata.org/wiki/Q618363","display_name":"Peripheral blood","level":2,"score":0.42797842621803284},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40820351243019104},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.33475783467292786},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3228623867034912},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3163319528102875},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.18089255690574646},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07178765535354614}],"mesh":[{"descriptor_ui":"D000433","descriptor_name":"1-Propanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D006146","descriptor_name":"Guanidines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007962","descriptor_name":"Leukocytes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013861","descriptor_name":"Thiocyanates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017879","descriptor_name":"Isothiocyanates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/0003-2697(88)90047-4","doi":"https://doi.org/10.1016/0003-2697(88)90047-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/0003-2697(88)90047-4","pdf_url":null,"source":{"id":"https://openalex.org/S7858788","display_name":"Analytical Biochemistry","issn_l":"0003-2697","issn":["0003-2697","1096-0309"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Biochemistry","raw_type":"journal-article"},{"id":"pmid:3239751","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3239751","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5410010676","display_name":null,"funder_award_id":"5R01 NS23816-02","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G8478244825","display_name":"NEURITE-PROMOTING FACTORS FROM MUSCLE AND AUTOIMMUNITY","funder_award_id":"R01NS023816","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W1938555178","https://openalex.org/W2009263927","https://openalex.org/W2014669590","https://openalex.org/W2038718616","https://openalex.org/W2068446734","https://openalex.org/W2122385641","https://openalex.org/W2224929038"],"related_works":["https://openalex.org/W2360450514","https://openalex.org/W2084841290","https://openalex.org/W1994722228","https://openalex.org/W2133826860","https://openalex.org/W2470402671","https://openalex.org/W2112132696","https://openalex.org/W2053454488","https://openalex.org/W2413877914","https://openalex.org/W2795511012","https://openalex.org/W2048481184"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}