{"id":"https://openalex.org/W2006058818","doi":"https://doi.org/10.1093/nar/19.21.5895","title":"FLP-mediated recombination in the vector mosquite,Aedes aegypti","display_name":"FLP-mediated recombination in the vector mosquite,Aedes aegypti","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W2006058818","doi":"https://doi.org/10.1093/nar/19.21.5895","mag":"2006058818","pmid":"https://pubmed.ncbi.nlm.nih.gov/1945877","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/329044"},"language":"en","primary_location":{"id":"doi:10.1093/nar/19.21.5895","doi":"https://doi.org/10.1093/nar/19.21.5895","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/19.21.5895","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC329044","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Alison C. Morris","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alison C. Morris","raw_affiliation_strings":["Department of Molecular Biology & Biochemistry, University of California, Irvine 92717","Dept. of Molecular Biology and Biochemistry, University of California, Irvine 92717"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology & Biochemistry, University of California, Irvine 92717","institution_ids":["https://openalex.org/I204250578"]},{"raw_affiliation_string":"Dept. of Molecular Biology and Biochemistry, University of California, Irvine 92717","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084971629","display_name":"Theresa L. Schaub","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Theresa L. Schaub","raw_affiliation_strings":["Department of Molecular Biology & Biochemistry, Unviersity of California Irvine, CA 92717, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology & Biochemistry, Unviersity of California Irvine, CA 92717, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039756363","display_name":"Anthony Amade James","orcid":"https://orcid.org/0000-0001-5577-3308","observed_orcids":["https://orcid.org/0000-0001-5577-3308"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anthony A. James","raw_affiliation_strings":["Department of Molecular Biology & Biochemistry, Unviersity of California Irvine, CA 92717, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology & Biochemistry, Unviersity of California Irvine, CA 92717, USA","institution_ids":["https://openalex.org/I204250578"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204250578"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":1.7723,"has_fulltext":false,"cited_by_count":61,"citation_normalized_percentile":{"value":0.84881961,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"19","issue":"21","first_page":"5895","last_page":"5900"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8730000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8730000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.02810000069439411,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.01720000058412552,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/flp-recombinase","display_name":"Flp recombinase","score":0.9259999990463257},{"id":"https://openalex.org/keywords/frt-sites","display_name":"FRT sites","score":0.8479999899864197},{"id":"https://openalex.org/keywords/site-specific-recombination","display_name":"site-specific recombination","score":0.8230000138282776},{"id":"https://openalex.org/keywords/aedes-aegypti","display_name":"Aedes aegypti","score":0.9879999756813049},{"id":"https://openalex.org/keywords/2-micron-plasmid","display_name":"2 micron plasmid","score":0.734000027179718},{"id":"https://openalex.org/keywords/heat-shock-promoter","display_name":"heat shock promoter","score":0.7789999842643738},{"id":"https://openalex.org/keywords/plasmid-excision","display_name":"plasmid excision","score":0.7419999837875366},{"id":"https://openalex.org/keywords/vector-control","display_name":"vector control","score":0.9559999704360962}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8970190286636353},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.8597423434257507},{"id":"https://openalex.org/C135300049","wikidata":"https://www.wikidata.org/wiki/Q5426559","display_name":"FLP-FRT recombination","level":5,"score":0.7711585760116577},{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.7447403073310852},{"id":"https://openalex.org/C119898374","wikidata":"https://www.wikidata.org/wiki/Q7531737","display_name":"Site-specific recombination","level":5,"score":0.7255280017852783},{"id":"https://openalex.org/C2777775583","wikidata":"https://www.wikidata.org/wiki/Q1148004","display_name":"Aedes aegypti","level":3,"score":0.602098822593689},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.5955203771591187},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.5771579146385193},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.5758817195892334},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5359896421432495},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.477792352437973},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.4400293231010437},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.42511826753616333},{"id":"https://openalex.org/C125929170","wikidata":"https://www.wikidata.org/wiki/Q908966","display_name":"Cre recombinase","level":5,"score":0.4189510941505432},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.3944116532802582},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3536871671676636},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3375549018383026},{"id":"https://openalex.org/C101434241","wikidata":"https://www.wikidata.org/wiki/Q211675","display_name":"Genetic recombination","level":4,"score":0.25162598490715027},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.08250322937965393},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.0},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000330","descriptor_name":"Aedes","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D000330","descriptor_name":"Aedes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004254","descriptor_name":"DNA Nucleotidyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004254","descriptor_name":"DNA Nucleotidyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006360","descriptor_name":"Heat-Shock Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007303","descriptor_name":"Insect Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009692","descriptor_name":"Nucleic Acid Heteroduplexes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012091","descriptor_name":"Repetitive Sequences, Nucleic Acid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/19.21.5895","doi":"https://doi.org/10.1093/nar/19.21.5895","is_oa":false,"landing_page_url":"https://doi.org/10.1093/nar/19.21.5895","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:41574","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC329044","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:329044","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/329044","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:1945877","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1945877","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:41574","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC329044","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1571154559","https://openalex.org/W1967446284","https://openalex.org/W1969022737","https://openalex.org/W1971855104","https://openalex.org/W1972687050","https://openalex.org/W1990748978","https://openalex.org/W2012674191","https://openalex.org/W2025073607","https://openalex.org/W2027755496","https://openalex.org/W2032776824","https://openalex.org/W2037356691","https://openalex.org/W2057009037","https://openalex.org/W2073493213","https://openalex.org/W2084495709","https://openalex.org/W2111459950","https://openalex.org/W2141653906","https://openalex.org/W2152845055","https://openalex.org/W2414836167","https://openalex.org/W4296765338"],"related_works":["https://openalex.org/W2008946269","https://openalex.org/W2012674191","https://openalex.org/W2399706259","https://openalex.org/W2000346699","https://openalex.org/W2086060314","https://openalex.org/W2526257590","https://openalex.org/W4231355921","https://openalex.org/W4232131685","https://openalex.org/W2120701413","https://openalex.org/W1992979468"],"abstract_inverted_index":{"The":[0,115],"activity":[1,146],"of":[2,18,27,36,63,70,86,108,142,156,163],"a":[3,25,37,106,152],"yeast":[4,102,135,143],"recombinase,":[5],"FLP,":[6],"on":[7,79],"specific":[8,71],"target":[9,45,122],"DNA":[10,72],"sequences,":[11],"FRT,":[12],"has":[13],"been":[14],"demonstrated":[15],"in":[16,83,147,159,167],"embryos":[17],"the":[19,31,68,84,101,134,148],"vector":[20,168],"mosquito,":[21],"Aedes":[22],"aegypti.":[23],"In":[24,93],"series":[26],"experiments,":[28],"plasmids":[29,46,81],"containing":[30,47],"FLP":[32,144],"recombinase":[33,145],"under":[34],"control":[35,169],"heterologous":[38],"heat-shock":[39],"gene":[40],"promoter":[41],"were":[42,112,118],"co-injected":[43],"with":[44],"FRT":[48,64,76,96,110],"sites":[49,65,77,97,111,117,123],"into":[50],"preblastoderm":[51],"stage":[52],"mosquito":[53,149],"embryos.":[54],"FLP-mediated":[55],"recombination":[56,125],"was":[57],"detected":[58],"between":[59],"(i)":[60],"tandem":[61],"repeats":[62],"leading":[66],"to":[67,95,130],"excision":[69],"sequences":[73],"and":[74,126,171],"(ii)":[75],"located":[78],"separate":[80],"resulting":[82],"formation":[85],"heterodimeric":[87],"or":[88],"higher":[89],"order":[90],"multimeric":[91],"plasmids.":[92],"addition":[94],"originally":[98],"isolated":[99],"from":[100,133],"2":[103,136],"microns":[104,137],"plasmid,":[105],"number":[107],"synthetic":[109,116],"also":[113],"used.":[114],"fully":[119],"functional":[120],"as":[121],"for":[124,165],"gave":[127],"results":[128],"similar":[129],"those":[131],"derived":[132],"plasmid.":[138],"This":[139],"successful":[140],"demonstration":[141],"embryo":[150],"suggests":[151],"possible":[153],"future":[154],"application":[155],"this":[157],"system":[158],"establishing":[160],"transformed":[161],"lines":[162],"mosquitoes":[164],"use":[166],"strategies":[170],"basic":[172],"studies.":[173]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}