{"id":"https://openalex.org/W2006050659","doi":"https://doi.org/10.1021/bi00839a053","title":"Thermodynamics of the denaturation of lysozyme by guanidine hydrochloride. II. Dependence on denaturant concentration at 25\u00b0","display_name":"Thermodynamics of the denaturation of lysozyme by guanidine hydrochloride. II. Dependence on denaturant concentration at 25\u00b0","publication_year":1969,"publication_date":"1969-11-01","ids":{"openalex":"https://openalex.org/W2006050659","doi":"https://doi.org/10.1021/bi00839a053","mag":"2006050659","pmid":"https://pubmed.ncbi.nlm.nih.gov/5353115"},"language":"en","primary_location":{"id":"doi:10.1021/bi00839a053","doi":"https://doi.org/10.1021/bi00839a053","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00839a053","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000987315","display_name":"Kirk C. Aune","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kirk C. Aune","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5054077827","display_name":"Charles Tanford","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Charles Tanford","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":2.8006,"has_fulltext":false,"cited_by_count":266,"citation_normalized_percentile":{"value":0.90562601,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"8","issue":"11","first_page":"4586","last_page":"4590"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.5185999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.5185999870300293,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.4357999861240387,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.028599999845027924,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lysozyme","display_name":"lysozyme","score":0.9919999837875366},{"id":"https://openalex.org/keywords/guanidine-hydrochloride","display_name":"guanidine hydrochloride","score":0.9380000233650208},{"id":"https://openalex.org/keywords/protein-denaturation","display_name":"protein denaturation","score":0.9860000014305115},{"id":"https://openalex.org/keywords/denaturant-concentration-dependence","display_name":"denaturant concentration dependence","score":0.953000009059906},{"id":"https://openalex.org/keywords/protein-unfolding","display_name":"protein unfolding","score":0.3779999911785126},{"id":"https://openalex.org/keywords/25-c","display_name":"25 \u00b0C","score":0.6740000247955322}],"concepts":[{"id":"https://openalex.org/C2779463914","wikidata":"https://www.wikidata.org/wiki/Q183309","display_name":"Guanidine","level":2,"score":0.7747341990470886},{"id":"https://openalex.org/C2776966407","wikidata":"https://www.wikidata.org/wiki/Q24779410","display_name":"Lysozyme","level":2,"score":0.6234122514724731},{"id":"https://openalex.org/C2778805511","wikidata":"https://www.wikidata.org/wiki/Q1713","display_name":"Citation","level":2,"score":0.5889623165130615},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5459770560264587},{"id":"https://openalex.org/C2776460385","wikidata":"https://www.wikidata.org/wiki/Q420060","display_name":"Hydrochloride","level":2,"score":0.4667525589466095},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3708413243293762},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.24968871474266052},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.175797700881958}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002713","descriptor_name":"Chlorine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004355","descriptor_name":"Drug Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006146","descriptor_name":"Guanidines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008433","descriptor_name":"Mathematics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009113","descriptor_name":"Muramidase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00839a053","doi":"https://doi.org/10.1021/bi00839a053","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00839a053","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:5353115","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5353115","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W2029589255","https://openalex.org/W2032373523","https://openalex.org/W2074593810","https://openalex.org/W2091990417","https://openalex.org/W2315644272","https://openalex.org/W2321944123","https://openalex.org/W2322220572","https://openalex.org/W2335418470"],"related_works":["https://openalex.org/W4242249163","https://openalex.org/W2074593810","https://openalex.org/W2009099364","https://openalex.org/W2039890697","https://openalex.org/W4247942885","https://openalex.org/W2400495362","https://openalex.org/W1989828403","https://openalex.org/W1528676293","https://openalex.org/W1505459338","https://openalex.org/W2383242872"],"abstract_inverted_index":{"ADVERTISEMENT":[0],"RETURN":[1],"TO":[2],"ISSUEPREVArticleNEXTThermodynamics":[3],"of":[4,7,55,90,116],"the":[5,52,83,88,117,128,141,144,149,156,162],"denaturation":[6],"lysozyme":[8],"by":[9,97],"guanidine":[10],"hydrochloride.":[11],"II.":[12],"Dependence":[13],"on":[14,127,155],"denaturant":[15],"concentration":[16],"at":[17,135],"25\u00b0Kirk":[18],"C.":[19],"Aune":[20],"and":[21,65,70,99,143,160,176,178],"Charles":[22],"TanfordCite":[23],"this:":[24],"Biochemistry":[25],"1969,":[26],"8,":[27],"11,":[28],"4586\u20134590Publication":[29],"Date":[30],"(Print):November":[31],"1,":[32],"1969Publication":[33],"History":[34],"Published":[35],"online1":[36],"May":[37],"2002Published":[38],"inissue":[39],"1":[40],"November":[41,61],"1969https://pubs.acs.org/doi/10.1021/bi00839a053https://doi.org/10.1021/bi00839a053research-articleACS":[42],"PublicationsRequest":[43],"reuse":[44],"permissionsArticle":[45],"Views1182Altmetric-Citations210LEARN":[46],"ABOUT":[47],"THESE":[48],"METRICSArticle":[49],"Views":[50],"are":[51,74,87],"COUNTER-compliant":[53],"sum":[54],"full":[56],"text":[57],"article":[58,122],"downloads":[59],"since":[60],"2008":[62],"(both":[63],"PDF":[64],"HTML)":[66],"across":[67],"all":[68],"institutions":[69],"individuals.":[71],"These":[72],"metrics":[73],"regularly":[75],"updated":[76,100],"to":[77,82],"reflect":[78],"usage":[79],"leading":[80],"up":[81],"last":[84],"few":[85],"days.Citations":[86],"number":[89],"other":[91],"articles":[92],"citing":[93],"this":[94],"article,":[95],"calculated":[96],"Crossref":[98,106],"daily.":[101],"Find":[102,152],"more":[103,153],"information":[104,154],"about":[105,140],"citation":[107],"counts.The":[108],"Altmetric":[109,157],"Attention":[110,158],"Score":[111,159],"is":[112,164],"a":[113,120,133],"quantitative":[114],"measure":[115],"attention":[118],"that":[119],"research":[121],"has":[123],"received":[124],"online.":[125],"Clicking":[126],"donut":[129],"icon":[130],"will":[131],"load":[132],"page":[134],"altmetric.com":[136],"with":[137,172],"additional":[138],"details":[139],"score":[142,163],"social":[145],"media":[146],"presence":[147],"for":[148],"given":[150],"article.":[151],"how":[161],"calculated.":[165],"Share":[166,181],"Add":[167],"toView":[168],"InAdd":[169],"Full":[170],"Text":[171],"ReferenceAdd":[173],"Description":[174],"ExportRISCitationCitation":[175],"abstractCitation":[177],"referencesMore":[179],"Options":[180],"onFacebookTwitterWechatLinked":[182],"InRedditEmail":[183],"Other":[184],"access":[185],"optionsGet":[186],"e-Alertsclose":[187],"Get":[188],"e-Alerts":[189]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}