{"id":"https://openalex.org/W2005692553","doi":"https://doi.org/10.3103/s0027131414020023","title":"Alteration of the formate dehydrogenase isoelectric point by rational design","display_name":"Alteration of the formate dehydrogenase isoelectric point by rational design","publication_year":2014,"publication_date":"2014-03-01","ids":{"openalex":"https://openalex.org/W2005692553","doi":"https://doi.org/10.3103/s0027131414020023","mag":"2005692553"},"language":"en","primary_location":{"id":"doi:10.3103/s0027131414020023","doi":"https://doi.org/10.3103/s0027131414020023","is_oa":false,"landing_page_url":"https://doi.org/10.3103/s0027131414020023","pdf_url":null,"source":{"id":"https://openalex.org/S84484310","display_name":"Moscow University Chemistry Bulletin","issn_l":"0027-1314","issn":["0027-1314","1935-0260"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["dongbi-c","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Moscow University Chemistry Bulletin","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057747338","display_name":"Anna V. Alekseeva","orcid":"https://orcid.org/0000-0002-0422-8382","observed_orcids":["https://orcid.org/0000-0002-0422-8382"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]},{"id":"https://openalex.org/I4210087281","display_name":"A N Bach Institute of Biochemistry","ror":"https://ror.org/0009wsb17","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210087281","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"A. A. Alekseeva","raw_affiliation_strings":["Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii prosp. 33, Moscow, 119074, Russia","Innovations and High Technologies MSU Ltd., ul. Tsymlyanskaya 16, Moscow, 109559, Russia","Russian Academy of Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii prosp. 33, Moscow, 119074, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210087281"]},{"raw_affiliation_string":"Innovations and High Technologies MSU Ltd., ul. Tsymlyanskaya 16, Moscow, 109559, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Russian Academy of Sciences","institution_ids":["https://openalex.org/I1313323035"]}]},{"author_position":"middle","author":{"id":null,"display_name":"A. S. Petrov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"A. S. Petrov","raw_affiliation_strings":["Department of Chemistry, Moscow State University, Moscow, 119991, Russia","Department of Chemistry , Moscow State University , Moscow , Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Moscow State University, Moscow, 119991, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Department of Chemistry , Moscow State University , Moscow , Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113906416","display_name":"Vitaly V. Fedorchuk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"V. V. Fedorchuk","raw_affiliation_strings":["Department of Chemistry, Moscow State University, Moscow, 119991, Russia","Department of Chemistry , Moscow State University , Moscow , Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Moscow State University, Moscow, 119991, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Department of Chemistry , Moscow State University , Moscow , Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066451748","display_name":"E. A. Fedorchuk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"E. A. Fedorchuk","raw_affiliation_strings":["Department of Chemistry, Moscow State University, Moscow, 119991, Russia","Department of Chemistry , Moscow State University , Moscow , Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Moscow State University, Moscow, 119991, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Department of Chemistry , Moscow State University , Moscow , Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"middle","author":{"id":null,"display_name":"T. A. Osipova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"T. A. Osipova","raw_affiliation_strings":["Department of Chemistry, Moscow State University, Moscow, 119991, Russia","Department of Chemistry , Moscow State University , Moscow , Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Moscow State University, Moscow, 119991, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Department of Chemistry , Moscow State University , Moscow , Russia","institution_ids":["https://openalex.org/I19880235"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084347593","display_name":"Vladimir I. Tishkov","orcid":"https://orcid.org/0000-0002-6997-7229","observed_orcids":["https://orcid.org/0000-0002-6997-7229"]},"institutions":[{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]},{"id":"https://openalex.org/I4210087281","display_name":"A N Bach Institute of Biochemistry","ror":"https://ror.org/0009wsb17","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210087281","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"V. I. Tishkov","raw_affiliation_strings":["Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii prosp. 33, Moscow, 119074, Russia","Department of Chemistry, Moscow State University, Moscow, 119991, Russia","Innovations and High Technologies MSU Ltd., ul. Tsymlyanskaya 16, Moscow, 109559, Russia","Moscow State University","Russian Academy of Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii prosp. 33, Moscow, 119074, Russia","institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I4210087281"]},{"raw_affiliation_string":"Department of Chemistry, Moscow State University, Moscow, 119991, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Innovations and High Technologies MSU Ltd., ul. Tsymlyanskaya 16, Moscow, 109559, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Moscow State University","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Russian Academy of Sciences","institution_ids":["https://openalex.org/I1313323035"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5084347593"],"corresponding_institution_ids":["https://openalex.org/I1313323035","https://openalex.org/I19880235","https://openalex.org/I4210087281"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.08205851,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"69","issue":"2","first_page":"73","last_page":"79"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9657999873161316,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9657999873161316,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.01489999983459711,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0071000000461936,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/formate-dehydrogenase","display_name":"formate dehydrogenase","score":0.9700000286102295},{"id":"https://openalex.org/keywords/isoelectric-point","display_name":"isoelectric point","score":0.8600000143051147},{"id":"https://openalex.org/keywords/ph-stability","display_name":"pH stability","score":0.8100000023841858},{"id":"https://openalex.org/keywords/nad-dependent-enzyme","display_name":"NAD-dependent enzyme","score":0.7039999961853027},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.9570000171661377},{"id":"https://openalex.org/keywords/lysine-substitution","display_name":"lysine substitution","score":0.7720000147819519},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.6840000152587891},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.8849999904632568},{"id":"https://openalex.org/keywords/rational-protein-design","display_name":"rational protein design","score":0.5860000252723694}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8773375749588013},{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.7501983642578125},{"id":"https://openalex.org/C2778428456","wikidata":"https://www.wikidata.org/wiki/Q1147563","display_name":"Formate dehydrogenase","level":4,"score":0.7275922894477844},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.726116955280304},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5636293888092041},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5507616996765137},{"id":"https://openalex.org/C2780178953","wikidata":"https://www.wikidata.org/wiki/Q2001841","display_name":"Formate","level":3,"score":0.523648738861084},{"id":"https://openalex.org/C59061564","wikidata":"https://www.wikidata.org/wiki/Q7783071","display_name":"Thermal stability","level":2,"score":0.5182404518127441},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4875456690788269},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.44623783230781555},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.4418047070503235},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.4339636564254761},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.4177211821079254},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.413299560546875},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0998249351978302},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.060438841581344604},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.055429697036743164}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3103/s0027131414020023","doi":"https://doi.org/10.3103/s0027131414020023","is_oa":false,"landing_page_url":"https://doi.org/10.3103/s0027131414020023","pdf_url":null,"source":{"id":"https://openalex.org/S84484310","display_name":"Moscow University Chemistry Bulletin","issn_l":"0027-1314","issn":["0027-1314","1935-0260"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["dongbi-c","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Moscow University Chemistry Bulletin","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.6800000071525574,"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W134373167","https://openalex.org/W2025965136","https://openalex.org/W2027960849","https://openalex.org/W2074011909","https://openalex.org/W2076803689","https://openalex.org/W2093027906","https://openalex.org/W2142902729","https://openalex.org/W2147886339","https://openalex.org/W2148966062","https://openalex.org/W2170393641"],"related_works":["https://openalex.org/W4366957169","https://openalex.org/W4379010599","https://openalex.org/W2027960849","https://openalex.org/W4224210441","https://openalex.org/W2140553270","https://openalex.org/W2321985368","https://openalex.org/W2172188445","https://openalex.org/W2124786659","https://openalex.org/W4293859199","https://openalex.org/W2160539801"],"abstract_inverted_index":{"In":[0],"order":[1],"to":[2],"extend":[3],"the":[4,18,42,46,55,67,70,92,101,105,113,120,125,139],"pH":[5],"stability":[6,80,117],"optimum":[7],"for":[8,83,112,119,159],"NAD":[9],"+":[10],"-dependent":[11],"formate":[12],"dehydrogenase":[13],"(FDH,":[14],"EC":[15],"1.2.1.2)":[16],"from":[17,63],"bacterium":[19],"Pseudomonas":[20],"sp.":[21],"101":[22],"(PseFDH),":[23],"four":[24],"mutant":[25,86,107,162],"enzymes":[26],"with":[27],"Lys112Pro,":[28],"Lys231Ala,":[29],"Lys231Ser,":[30],"and":[31,45,66,78,97,130],"Lys317Asn":[32,106,142],"substitutions":[33,93,126],"were":[34,52,81],"obtained":[35,85,121,161],"by":[36,156],"site-directed":[37],"mutagenesis.":[38],"The":[39,75,151],"choice":[40],"of":[41,48,57,61,69,73,138],"mutation":[43],"sites":[44],"types":[47],"substituting":[49],"amino":[50,58],"acids":[51],"based":[53],"on":[54],"alignment":[56],"acid":[59],"sequences":[60],"FDHs":[62],"various":[64],"sources":[65],"analysis":[68],"three-dimensional":[71],"structure":[72],"PseFDH.":[74],"kinetic":[76,102],"properties":[77],"temperature":[79],"studied":[82],"all":[84,160],"forms.":[87,163],"It":[88],"is":[89],"shown":[90],"that":[91,124],"in":[94,127,133,148],"positions":[95,128],"112":[96,129],"231":[98,131],"slightly":[99],"improved":[100],"properties;":[103],"meanwhile,":[104],"possessed":[108],"a":[109,135,145],"decreased":[110,155],"affinity":[111],"coenzyme.":[114],"A":[115],"thermal":[116,149],"assay":[118],"mutants":[122],"revealed":[123],"result":[132],"just":[134],"slight":[136],"destabilization":[137],"enzyme,":[140],"while":[141],"substitution":[143],"causes":[144],"significant":[146],"decrease":[147],"stability.":[150],"isoelectric":[152],"point":[153],"was":[154],"0.1":[157],"points":[158]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}
