{"id":"https://openalex.org/W2005668729","doi":"https://doi.org/10.1002/bip.20010","title":"Study of protein aggregation using two\u2010dimensional correlation infrared spectroscopy and spectral simulations","display_name":"Study of protein aggregation using two\u2010dimensional correlation infrared spectroscopy and spectral simulations","publication_year":2004,"publication_date":"2004-03-08","ids":{"openalex":"https://openalex.org/W2005668729","doi":"https://doi.org/10.1002/bip.20010","mag":"2005668729","pmid":"https://pubmed.ncbi.nlm.nih.gov/15048774"},"language":"en","primary_location":{"id":"doi:10.1002/bip.20010","doi":"https://doi.org/10.1002/bip.20010","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bip.20010","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084093705","display_name":"Thierry Lef\u00e8vre","orcid":"https://orcid.org/0000-0003-2696-250X","observed_orcids":["https://orcid.org/0000-0003-2696-250X"]},"institutions":[{"id":"https://openalex.org/I43406934","display_name":"Universit\u00e9 Laval","ror":"https://ror.org/04sjchr03","country_code":"CA","type":"education","lineage":["https://openalex.org/I43406934"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Thierry Lef\u00e8vre","raw_affiliation_strings":["Centre de Recherche en Sciences et Ing\u00e9nierie des Macromol\u00e9cules, D\u00e9partement de Chimie, Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) G1K 7P4, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre de Recherche en Sciences et Ing\u00e9nierie des Macromol\u00e9cules, D\u00e9partement de Chimie, Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) G1K 7P4, Canada","institution_ids":["https://openalex.org/I43406934"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041090216","display_name":"Karin Arseneault","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43406934","display_name":"Universit\u00e9 Laval","ror":"https://ror.org/04sjchr03","country_code":"CA","type":"education","lineage":["https://openalex.org/I43406934"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Karin Arseneault","raw_affiliation_strings":["Centre de Recherche en Sciences et Inge\u0301nierie des Macromole\u0301cules, De\u0301partement de Chimie, Universite\u0301 Laval, Que\u0301bec (Que\u0301bec) G1K 7P4, Canada","Centre de Recherche en Sciences et Ing\u00e9nierie des Macromol\u00e9cules, D\u00e9partement de Chimie, Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) G1K 7P4, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre de Recherche en Sciences et Inge\u0301nierie des Macromole\u0301cules, De\u0301partement de Chimie, Universite\u0301 Laval, Que\u0301bec (Que\u0301bec) G1K 7P4, Canada","institution_ids":["https://openalex.org/I43406934"]},{"raw_affiliation_string":"Centre de Recherche en Sciences et Ing\u00e9nierie des Macromol\u00e9cules, D\u00e9partement de Chimie, Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) G1K 7P4, Canada","institution_ids":["https://openalex.org/I43406934"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029712266","display_name":"Michel P\u00e9zolet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43406934","display_name":"Universit\u00e9 Laval","ror":"https://ror.org/04sjchr03","country_code":"CA","type":"education","lineage":["https://openalex.org/I43406934"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Michel P\u00e9zolet","raw_affiliation_strings":["Centre de Recherche en Sciences et Inge\u0301nierie des Macromole\u0301cules, De\u0301partement de Chimie, Universite\u0301 Laval, Que\u0301bec (Que\u0301bec) G1K 7P4, Canada","Centre de Recherche en Sciences et Ing\u00e9nierie des Macromol\u00e9cules, D\u00e9partement de Chimie, Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) G1K 7P4, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre de Recherche en Sciences et Inge\u0301nierie des Macromole\u0301cules, De\u0301partement de Chimie, Universite\u0301 Laval, Que\u0301bec (Que\u0301bec) G1K 7P4, Canada","institution_ids":["https://openalex.org/I43406934"]},{"raw_affiliation_string":"Centre de Recherche en Sciences et Ing\u00e9nierie des Macromol\u00e9cules, D\u00e9partement de Chimie, Universit\u00e9 Laval, Qu\u00e9bec (Qu\u00e9bec) G1K 7P4, Canada","institution_ids":["https://openalex.org/I43406934"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5029712266"],"corresponding_institution_ids":["https://openalex.org/I43406934"],"apc_list":{"value":4150,"currency":"USD","value_usd":4150},"apc_paid":null,"fwci":2.8615,"has_fulltext":false,"cited_by_count":55,"citation_normalized_percentile":{"value":0.89453251,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"73","issue":"6","first_page":"705","last_page":"715"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.8393999934196472,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.8393999934196472,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11177","display_name":"Spectroscopy and Quantum Chemical Studies","score":0.06790000200271606,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0340999998152256,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.9940000176429749},{"id":"https://openalex.org/keywords/two-dimensional-correlation-spectroscopy","display_name":"two-dimensional correlation spectroscopy","score":0.8479999899864197},{"id":"https://openalex.org/keywords/spectral-effects","display_name":"spectral effects","score":0.7879999876022339},{"id":"https://openalex.org/keywords/asynchrony","display_name":"asynchrony","score":0.6639999747276306},{"id":"https://openalex.org/keywords/spectral-simulation","display_name":"spectral simulation","score":0.6610000133514404},{"id":"https://openalex.org/keywords/glutamyl-trna-synthetase","display_name":"glutamyl-tRNA synthetase","score":0.9240000247955322},{"id":"https://openalex.org/keywords/heat-induced-aggregation","display_name":"heat-induced aggregation","score":0.9200000166893005},{"id":"https://openalex.org/keywords/amide-i-region","display_name":"amide I region","score":0.824999988079071},{"id":"https://openalex.org/keywords/alpha-helix-loss","display_name":"alpha-helix loss","score":0.8050000071525574},{"id":"https://openalex.org/keywords/beta-sheet-formation","display_name":"\u03b2-sheet formation","score":0.8080000281333923},{"id":"https://openalex.org/keywords/protein-mutants","display_name":"protein mutants","score":0.5080000162124634}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7803301811218262},{"id":"https://openalex.org/C4839761","wikidata":"https://www.wikidata.org/wiki/Q212111","display_name":"Spectral line","level":2,"score":0.5921604633331299},{"id":"https://openalex.org/C32891209","wikidata":"https://www.wikidata.org/wiki/Q483666","display_name":"Spectroscopy","level":2,"score":0.5178523659706116},{"id":"https://openalex.org/C75079739","wikidata":"https://www.wikidata.org/wiki/Q1571801","display_name":"Intramolecular force","level":2,"score":0.5171374678611755},{"id":"https://openalex.org/C153642686","wikidata":"https://www.wikidata.org/wiki/Q70906","display_name":"Infrared spectroscopy","level":2,"score":0.4922312796115875},{"id":"https://openalex.org/C166950319","wikidata":"https://www.wikidata.org/wiki/Q245031","display_name":"Intermolecular force","level":3,"score":0.4714316427707672},{"id":"https://openalex.org/C160892712","wikidata":"https://www.wikidata.org/wiki/Q901559","display_name":"Fourier transform infrared spectroscopy","level":2,"score":0.4706791043281555},{"id":"https://openalex.org/C136238340","wikidata":"https://www.wikidata.org/wiki/Q7251455","display_name":"Protein aggregation","level":2,"score":0.4609648883342743},{"id":"https://openalex.org/C102519508","wikidata":"https://www.wikidata.org/wiki/Q6520159","display_name":"Fourier transform","level":2,"score":0.452517032623291},{"id":"https://openalex.org/C19549132","wikidata":"https://www.wikidata.org/wiki/Q11141463","display_name":"Two-dimensional nuclear magnetic resonance spectroscopy","level":2,"score":0.42817386984825134},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.3607505261898041},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.15214872360229492},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.1344422996044159},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.12636500597000122},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.12544453144073486},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08431011438369751},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005975","descriptor_name":"Glutamate-tRNA Ligase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011489","descriptor_name":"Protein Denaturation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017433","descriptor_name":"Protein Structure, Secondary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017550","descriptor_name":"Spectroscopy, Fourier Transform Infrared","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/bip.20010","doi":"https://doi.org/10.1002/bip.20010","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bip.20010","pdf_url":null,"source":{"id":"https://openalex.org/S56808608","display_name":"Biopolymers","issn_l":"0006-3525","issn":["0006-3525","1097-0282"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"journal-article"},{"id":"pmid:15048774","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15048774","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biopolymers","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321747","display_name":"Fonds Qu\u00e9b\u00e9cois de la Recherche sur la Nature et les Technologies","ror":"https://ror.org/00b9f9778"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1426595445","https://openalex.org/W1562919767","https://openalex.org/W1567873486","https://openalex.org/W1577843618","https://openalex.org/W1934853983","https://openalex.org/W1968924272","https://openalex.org/W1972390664","https://openalex.org/W1972436257","https://openalex.org/W1975372451","https://openalex.org/W1985635711","https://openalex.org/W1990391943","https://openalex.org/W1996185826","https://openalex.org/W2003744961","https://openalex.org/W2008257760","https://openalex.org/W2012886219","https://openalex.org/W2020372854","https://openalex.org/W2021997671","https://openalex.org/W2026559340","https://openalex.org/W2027342397","https://openalex.org/W2040286733","https://openalex.org/W2046053892","https://openalex.org/W2056615502","https://openalex.org/W2062102053","https://openalex.org/W2076459230","https://openalex.org/W2077231187","https://openalex.org/W2078766797","https://openalex.org/W2088107416","https://openalex.org/W2088730054","https://openalex.org/W2090413444","https://openalex.org/W2093085722","https://openalex.org/W2094123278","https://openalex.org/W2120116130","https://openalex.org/W2178012333","https://openalex.org/W3183637643"],"related_works":["https://openalex.org/W2112254638","https://openalex.org/W2003744961","https://openalex.org/W2392458854","https://openalex.org/W2249527323","https://openalex.org/W1969949749","https://openalex.org/W2271801786","https://openalex.org/W996929774","https://openalex.org/W2032484068","https://openalex.org/W1978940499","https://openalex.org/W2050154458"],"abstract_inverted_index":{"Two-dimensional":[0],"(2D)":[1],"correlation":[2],"spectroscopy":[3,97],"establishes":[4],"correlations":[5],"between":[6],"intensity":[7],"variations":[8],"in":[9,36,45,98,155,253,287],"a":[10,86,111,172],"series":[11],"of":[12,18,65,70,79,89,92,143,149,159,185,194,208,220,229,235,259,284],"spectra":[13,148,168],"obtained":[14,109],"by":[15,116],"the":[16,46,63,76,90,99,123,131,144,150,160,177,183,192,199,205,218,227,233,241,250,260,282],"application":[17,91],"an":[19,59],"external":[20],"perturbation.":[21],"However,":[22],"spectral":[23,40,178,267],"effects":[24,41,67],"(wavenumber":[25],"shift":[26],"or":[27],"bandwidth":[28,158],"change)":[29],"are":[30,42,126,244,252,263,278],"known":[31],"to":[32,55,61,137,162,212,223,266,272],"generate":[33],"apparent":[34],"asynchronisms":[35,72,277],"2D":[37,74,133],"maps.":[38,289],"Surprisingly,":[39],"often":[43],"neglected":[44],"literature":[47],"when":[48,247],"interpreting":[49],"experimental":[50,132,242],"maps,":[51,75],"which":[52,152],"can":[53],"lead":[54],"erroneous":[56],"conclusions.":[57],"In":[58,216],"attempt":[60],"evaluate":[62],"contribution":[64],"these":[66],"and":[68,146,157,182,201],"that":[69,114,175,196,257,275],"true":[71,276],"on":[73,140],"heat-induced":[77],"aggregation":[78,124,206],"glutamyl-tRNA":[80],"synthetase":[81],"(GluRS)":[82],"was":[83],"studied":[84],"as":[85],"typical":[87],"example":[88],"Fourier":[93],"transform":[94],"infrared":[95],"(FTIR)":[96],"amide":[100],"I":[101],"region.":[102],"The":[103,180],"data":[104],"were":[105,135,169,188],"compared":[106,136],"with":[107,226],"those":[108],"from":[110,191],"mutant":[112,200],"protein":[113],"differs":[115],"one":[117,270],"amino":[118],"acid.":[119],"To":[120],"determine":[121],"whether":[122],"mechanisms":[125,207],"identical":[127],"for":[128,198,203],"both":[129,209],"proteins,":[130],"maps":[134,243],"simulations":[138],"based":[139],"curve":[141],"fitting":[142],"initial":[145],"final":[147],"series,":[151],"allows":[153],"change":[154],"position":[156],"components":[161,251],"be":[163,213,224,273],"taken":[164],"into":[165],"account.":[166],"Intermediate":[167],"generated":[170],"using":[171],"convenient":[173],"function":[174],"mimics":[176],"evolution.":[179],"speed":[181],"delay":[184],"each":[186],"component":[187],"controlled.":[189],"Apart":[190],"appearance":[193],"turns":[195],"occur":[197],"not":[202,279],"GluRS,":[204],"proteins":[210],"seems":[211,222],"essentially":[214],"identical.":[215],"particular,":[217],"loss":[219,234],"alpha-helices":[221],"concomitant":[225],"formation":[228],"intermolecular":[230],"beta-sheets,":[231],"whereas":[232],"intramolecular":[236],"beta-sheets":[237],"is":[238],"delayed.":[239],"Since":[240],"satisfactorily":[245],"simulated":[246],"almost":[248],"all":[249],"phase,":[254],"it":[255],"appears":[256],"many":[258],"asynchronous":[261,288],"features":[262],"mainly":[264],"due":[265],"effects.":[268],"Thus,":[269],"has":[271],"aware":[274],"necessarily":[280],"at":[281],"origin":[283],"peaks":[285],"observed":[286]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}