{"id":"https://openalex.org/W2005601439","doi":"https://doi.org/10.1089/dna.1986.5.339","title":"A Method for Constructing Multiple Tandem Repeats of Specific DNA Fragments","display_name":"A Method for Constructing Multiple Tandem Repeats of Specific DNA Fragments","publication_year":1986,"publication_date":"1986-08-01","ids":{"openalex":"https://openalex.org/W2005601439","doi":"https://doi.org/10.1089/dna.1986.5.339","mag":"2005601439","pmid":"https://pubmed.ncbi.nlm.nih.gov/3017659"},"language":"en","primary_location":{"id":"doi:10.1089/dna.1986.5.339","doi":"https://doi.org/10.1089/dna.1986.5.339","is_oa":false,"landing_page_url":"https://doi.org/10.1089/dna.1986.5.339","pdf_url":null,"source":{"id":"https://openalex.org/S4210174578","display_name":"DNA","issn_l":"0198-0238","issn":["0198-0238","2331-4672"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"DNA","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"BRUCE COHEN","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"BRUCE COHEN","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5035971896","display_name":"Gordon Carmichael","orcid":"https://orcid.org/0000-0002-0379-6580","observed_orcids":["https://orcid.org/0000-0002-0379-6580"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"GORDON G. CARMICHAEL","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1439,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.40579181,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"5","issue":"4","first_page":"339","last_page":"343"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.43700000643730164,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.43700000643730164,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.2206999957561493,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.17919999361038208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tandem-repeats","display_name":"tandem repeats","score":0.8159999847412109},{"id":"https://openalex.org/keywords/cohesive-ends","display_name":"cohesive ends","score":0.8479999899864197},{"id":"https://openalex.org/keywords/replication-origins","display_name":"origin of replication","score":0.6060000061988831},{"id":"https://openalex.org/keywords/mouse-polyomavirus","display_name":"mouse polyomavirus","score":0.7979999780654907},{"id":"https://openalex.org/keywords/inverted-repeats","display_name":"inverted repeats","score":0.8069999814033508},{"id":"https://openalex.org/keywords/recombinant-dna-construction","display_name":"recombinant DNA construction","score":0.7609999775886536}],"concepts":[{"id":"https://openalex.org/C27149982","wikidata":"https://www.wikidata.org/wiki/Q3456514","display_name":"Tandem repeat","level":4,"score":0.7274580597877502},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.6914812326431274},{"id":"https://openalex.org/C2776235265","wikidata":"https://www.wikidata.org/wiki/Q18392052","display_name":"Fragment (logic)","level":2,"score":0.6233372092247009},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6175076961517334},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.5749858617782593},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.558570384979248},{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.5476292371749878},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.54245525598526},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.49206846952438354},{"id":"https://openalex.org/C2777814067","wikidata":"https://www.wikidata.org/wiki/Q1752317","display_name":"Tandem","level":2,"score":0.4909124970436096},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.48932498693466187},{"id":"https://openalex.org/C168374021","wikidata":"https://www.wikidata.org/wiki/Q1924179","display_name":"Cloning vector","level":5,"score":0.47734907269477844},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4641581177711487},{"id":"https://openalex.org/C84289048","wikidata":"https://www.wikidata.org/wiki/Q7316314","display_name":"Restriction fragment","level":3,"score":0.4270569384098053},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4091910719871521},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4036054313182831},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.2873755097389221},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.19289320707321167},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.1328202784061432},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12411674857139587},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.11543336510658264},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004742","descriptor_name":"Enhancer Elements, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D011120","descriptor_name":"Polyomavirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012091","descriptor_name":"Repetitive Sequences, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1089/dna.1986.5.339","doi":"https://doi.org/10.1089/dna.1986.5.339","is_oa":false,"landing_page_url":"https://doi.org/10.1089/dna.1986.5.339","pdf_url":null,"source":{"id":"https://openalex.org/S4210174578","display_name":"DNA","issn_l":"0198-0238","issn":["0198-0238","2331-4672"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"DNA","raw_type":"journal-article"},{"id":"pmid:3017659","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3017659","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"DNA (Mary Ann Liebert, Inc.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4562596086","display_name":null,"funder_award_id":"CA32325","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306078","display_name":"U.S. Department of Defense","ror":"https://ror.org/0447fe631"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W1506041904","https://openalex.org/W1601687245","https://openalex.org/W1967446284","https://openalex.org/W1989237565","https://openalex.org/W2001452629","https://openalex.org/W2016844058","https://openalex.org/W2066967151","https://openalex.org/W2075504099","https://openalex.org/W2138270253"],"related_works":["https://openalex.org/W81923626","https://openalex.org/W1988411481","https://openalex.org/W2010886491","https://openalex.org/W2005001323","https://openalex.org/W1969670839","https://openalex.org/W2036737317","https://openalex.org/W2084214797","https://openalex.org/W2065723356","https://openalex.org/W2392934502","https://openalex.org/W2375276127"],"abstract_inverted_index":{"We":[0],"describe":[1],"a":[2,24,50,65,68,184,190],"new":[3],"method":[4,33],"for":[5],"constructing":[6],"tandem":[7,20,150],"repeats":[8],"of":[9,19,31,60,89,114,136,143,152,155,158,165,178],"DNA":[10,27,37,156],"fragments":[11,38,83,94,121],"that":[12,52],"allows":[13],"one":[14,105],"to":[15,35,44,169],"control":[16],"the":[17,32,58,76,82,111,115,137,141,153,159],"number":[18],"copies":[21,151],"appearing":[22],"in":[23,48,78,110],"final":[25],"recombinant":[26],"clone.":[28],"The":[29,92,163],"principle":[30],"is":[34,62,174],"prepare":[36],"with":[39,67,75,95,104],"predetermined":[40],"cohesive":[41,97],"ends":[42,98,124],"and":[43,81,102,128],"ligate":[45],"them":[46],"together":[47],"such":[49],"way":[51],"unique":[53,191],"multimers":[54],"are":[55,73,84,99],"generated.":[56],"First,":[57],"fragment":[59,77],"interest":[61],"inserted":[63],"into":[64,130],"vector":[66,187],"multisite":[69],"cloning":[70,112,194],"region.":[71],"Clones":[72],"picked":[74],"both":[79],"orientations,":[80],"excised":[85],"using":[86,166],"different":[87],"pairs":[88],"restriction":[90],"enzymes.":[91],"resulting":[93],"preprogrammed":[96],"mixed,":[100],"ligated,":[101],"digested":[103],"enzyme":[106],"whose":[107],"site":[108],"appears":[109],"region":[113],"vector.":[116],"From":[117],"this":[118,167,179],"material":[119],"multimeric":[120],"containing":[122,145],"specific":[123],"can":[125],"be":[126],"isolated":[127],"recloned":[129],"appropriate":[131],"vectors.":[132],"As":[133,176],"an":[134],"example":[135],"method,":[138],"we":[139,181],"show":[140],"construction":[142],"clones":[144],"either":[146],"three":[147],"or":[148],"six":[149],"origin":[154],"replication":[157],"mouse":[160],"polyoma":[161],"virus.":[162],"prospect":[164],"technique":[168],"construct":[170],"inverted":[171],"repeat":[172],"structures":[173],"discussed.":[175],"part":[177],"work":[180],"have":[182],"constructed":[183],"modified":[185],"M13mp8":[186],"which":[188],"contains":[189],"Xho":[192],"I":[193],"site.":[195]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}