{"id":"https://openalex.org/W2005507239","doi":"https://doi.org/10.1158/1538-7445.am2014-3147","title":"Abstract 3147: Low oxygen-driven expression of KrasV12 controls a switch between lung tumor growth and migration","display_name":"Abstract 3147: Low oxygen-driven expression of KrasV12 controls a switch between lung tumor growth and migration","publication_year":2014,"publication_date":"2014-10-01","ids":{"openalex":"https://openalex.org/W2005507239","doi":"https://doi.org/10.1158/1538-7445.am2014-3147","mag":"2005507239"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2014-3147","doi":"https://doi.org/10.1158/1538-7445.am2014-3147","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2014-3147","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-3","ki-jl-3","medline","norway-2"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021279758","display_name":"Anette Claudia Schafer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anette C. Schafer","raw_affiliation_strings":["UTSW Medical Center, Dallas, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UTSW Medical Center, Dallas, TX","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081012778","display_name":"Jerry W. Shay","orcid":"https://orcid.org/0000-0001-5052-0627","observed_orcids":["https://orcid.org/0000-0001-5052-0627"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jerry W. Shay","raw_affiliation_strings":["UTSW Medical Center, Dallas, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UTSW Medical Center, Dallas, TX","institution_ids":["https://openalex.org/I867280407"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037958980","display_name":"Gaudenz M. Danuser","orcid":"https://orcid.org/0000-0001-8583-2014","observed_orcids":["https://orcid.org/0000-0001-8583-2014"]},"institutions":[{"id":"https://openalex.org/I867280407","display_name":"The University of Texas Southwestern Medical Center","ror":"https://ror.org/05byvp690","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I867280407"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gaudenz Danuser","raw_affiliation_strings":["UTSW Medical Center, Dallas, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UTSW Medical Center, Dallas, TX","institution_ids":["https://openalex.org/I867280407"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I867280407"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"74","issue":"19_Supplement","first_page":"3147","last_page":"3147"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.6723999977111816,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.6723999977111816,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.19099999964237213,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10631","display_name":"Cancer, Hypoxia, and Metabolism","score":0.033900000154972076,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lung-cancer","display_name":"lung cancer","score":0.7279999852180481},{"id":"https://openalex.org/keywords/hypoxia","display_name":"hypoxia","score":0.8169999718666077},{"id":"https://openalex.org/keywords/tumor-cell-migration","display_name":"tumor cell migration","score":0.8510000109672546},{"id":"https://openalex.org/keywords/tumor-growth","display_name":"tumor growth","score":0.7160000205039978},{"id":"https://openalex.org/keywords/cell-morphology","display_name":"cell morphology","score":0.5879999995231628},{"id":"https://openalex.org/keywords/extracellular-matrix","display_name":"extracellular matrix","score":0.6769999861717224},{"id":"https://openalex.org/keywords/collagen","display_name":"collagen","score":0.8700000047683716},{"id":"https://openalex.org/keywords/cell-adhesion","display_name":"cell adhesion","score":0.5809999704360962},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.460999995470047},{"id":"https://openalex.org/keywords/metastasis","display_name":"metastasis","score":0.6100000143051147},{"id":"https://openalex.org/keywords/oncogenic-kras","display_name":"oncogenic KRAS","score":0.4699999988079071},{"id":"https://openalex.org/keywords/tumor-spheroids","display_name":"tumor spheroids","score":0.4880000054836273},{"id":"https://openalex.org/keywords/3d-collagen-matrix","display_name":"3D collagen matrix","score":0.7080000042915344}],"concepts":[{"id":"https://openalex.org/C2781187634","wikidata":"https://www.wikidata.org/wiki/Q20969980","display_name":"KRAS","level":4,"score":0.9487274885177612},{"id":"https://openalex.org/C127561419","wikidata":"https://www.wikidata.org/wiki/Q224527","display_name":"Downregulation and upregulation","level":3,"score":0.5259299874305725},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5129691958427429},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.49785327911376953},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4964790940284729},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4922775328159332},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.46123600006103516},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4555538594722748},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.4321586489677429},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4004892408847809},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38523057103157043},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.102772057056427},{"id":"https://openalex.org/C526805850","wikidata":"https://www.wikidata.org/wiki/Q188874","display_name":"Colorectal cancer","level":3,"score":0.09890416264533997},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.051726073026657104}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2014-3147","doi":"https://doi.org/10.1158/1538-7445.am2014-3147","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2014-3147","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-3","ki-jl-3","medline","norway-2"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3030972949","https://openalex.org/W4306404900","https://openalex.org/W4229832877","https://openalex.org/W4248366929","https://openalex.org/W3111812619","https://openalex.org/W3016435787","https://openalex.org/W1985998670","https://openalex.org/W2072481810","https://openalex.org/W2347992620","https://openalex.org/W4307860292"],"abstract_inverted_index":{"Abstract":[0],"Cancer":[1,475,486],"cell":[2,10,48,80,90,267,286,378],"migration":[3,49,189,237,335,379],"in":[4,38,56,108,118,179,202,217,280,339,410],"vivo":[5],"occurs":[6],"at":[7,246],"the":[8,20,125,162,170,247,250,254,283,294,307,324,466,471],"single":[9,159],"level":[11,171],"or":[12],"as":[13,47,424],"a":[14,28,35,57,87,115,140,265,288,340,363,372,381,394,427,454],"chain":[15],"of":[16,25,32,60,120,135,155,158,172,177,185,208,234,249,291,310,318,333,391,408,451,465,470],"motile":[17,404],"cells":[18,127,210,245,304,322,405,415],"leaving":[19],"primary":[21],"tumor":[22,458],"site.":[23],"Mutation":[24],"Kras":[26,41,75,100,178,192,198,224,292,300,312,328,354,360,385,392,409,418],"to":[27,66,124,212,264,403,431],"constituitively":[29],"active":[30,54],"form":[31,213,332],"Kras,":[33],"is":[34],"common":[36],"alteration":[37],"lung":[39,88,320,457],"cancer.":[40],"controls":[42,453],"many":[43],"cellular":[44],"processes":[45],"such":[46,281],"and":[50,69,166,169,236,347,399,435,460],"proliferation.":[51],"Being":[52],"constantly":[53],"results":[55],"hyper":[58],"activation":[59,76],"downstream":[61],"signaling":[62],"cascades,":[63],"which":[64,93],"lead":[65],"enhanced":[67],"tumorigenicity":[68],"high":[70,197,299,311,359,389,417,421],"metastatic":[71],"potential.":[72],"How":[73],"constitutive":[74],"translates":[77],"into":[78,146],"these":[79,180,209,243,319,368],"functions,":[81],"however,":[82],"has":[83],"remained":[84,200],"unclear.":[85],"Using":[86,273],"cancer":[89,321,377],"transformation":[91],"model,":[92],"expresses":[94],"oncogenic":[95],"KrasV12,":[96],"we":[97,276,314,350,370],"discovered":[98],"that":[99,268,279,316,352],"expression":[101,176,290,450],"got":[102],"substantially":[103],"upregulated":[104],"under":[105,129],"long-term":[106],"culture":[107,216],"2%":[109],"O2.":[110],"This":[111,149,330],"was":[112,151,230,336],"accompanied":[113],"by":[114,215,232,380],"significant":[116],"increase":[117,229],"growth":[119,459],"tumor-like":[121],"aggregates":[122],"compared":[123],"same":[126,308],"cultured":[128],"atmospheric":[130],"21%":[131],"O2":[132],"conditions.":[133],"Up-regulation":[134],"KrasV12":[136,173,452],"also":[137,337],"correlated":[138,187],"with":[139,188,323,412,420],"morphological":[141,182],"change":[142],"from":[143,306],"adherent":[144,165],"single-cells":[145],"sphere-like":[147,167],"aggregates.":[148,203],"morphology":[150],"reversible":[152,231],"upon":[153,406],"knock-down":[154],"Kras.":[156],"Separation":[157],"clones":[160,186,349],"confirmed":[161],"relation":[163],"between":[164,456],"morphologies":[168],"expression.":[174,329],"The":[175],"two":[181],"different":[183],"types":[184],"behavior.":[190],"Low":[191,448],"expressers":[193,199,313,355,361,419],"were":[194],"mobile":[195],"whereas":[196,293],"stationary":[201],"In":[204],"agreement,":[205],"protein":[206],"analysis":[207],"forced":[211],"spheres":[214],"low":[218,289,353,382],"adhesion":[219,235],"dishes":[220],"dramatically":[221],"increased":[222],"their":[223],"content.":[225],"Most":[226],"interestingly,":[227],"this":[228],"stimulation":[233],"on":[238,367],"collagen":[239,342,364],"coated":[240],"substrates.":[241],"Under":[242],"conditions,":[244],"periphery":[248],"aggregate":[251,309],"migrate":[252,432],"onto":[253],"surrounding":[255],"extracellular":[256],"matrix":[257],"(ECM).":[258],"Curiously,":[259],"they":[260],"often":[261],"stayed":[262],"attached":[263],"second":[266],"did":[269],"not":[270],"actively":[271,284,356],"migrate.":[272],"immunofluorescence":[274],"imaging":[275],"could":[277],"identify":[278],"pairs":[282],"migrating":[285],"had":[287],"passive":[295],"\u2018cargo":[296],"cell\u2019":[297],"retained":[298],"levels.":[301],"As":[302],"both":[303],"originated":[305],"conclude":[315],"interaction":[317],"ECM":[325],"negatively":[326],"regulates":[327],"new":[331],"combined":[334],"observed":[338],"3D":[341],"matrix.":[343],"By":[344],"co-culturing":[345],"high-":[346],"low-Kras":[348],"showed":[351],"haul":[357],"non-motile,":[358],"through":[362],"scaffold.":[365],"Based":[366],"findings":[369],"propose":[371],"novel":[373],"mechanism":[374],"for":[375,474],"controlling":[376],"O2-driven,":[383],"intrinsic":[384],"switch.":[386],"Cells":[387],"expressing":[388],"levels":[390],"show":[393],"more":[395],"sphere-like,":[396],"non-motile":[397],"phenotype":[398],"can":[400],"give":[401],"rise":[402],"down-regulation":[407],"contact":[411],"ECM.":[413],"These":[414],"carry":[416],"tumorigenic":[422],"potential":[423],"cargo":[425],"generating":[426],"dangerous":[428],"team":[429],"able":[430],"out,":[433],"spread,":[434],"regrow":[436],"elsewhere.":[437],"Citation":[438],"Format:":[439],"Anette":[440],"C.":[441],"Schafer,":[442],"Jerry":[443],"W.":[444],"Shay,":[445],"Gaudenz":[446],"Danuser.":[447],"oxygen-driven":[449],"switch":[455],"migration.":[461],"[abstract].":[462],"In:":[463],"Proceedings":[464],"105th":[467],"Annual":[468],"Meeting":[469],"American":[472],"Association":[473],"Research;":[476],"2014":[477],"Apr":[478],"5-9;":[479],"San":[480],"Diego,":[481],"CA.":[482],"Philadelphia":[483],"(PA):":[484],"AACR;":[485],"Res":[487],"2014;74(19":[488],"Suppl):Abstract":[489],"nr":[490],"3147.":[491],"doi:10.1158/1538-7445.AM2014-3147":[492]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2025-10-10T00:00:00"}