{"id":"https://openalex.org/W2005341061","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb16314.x","title":"Purification and cDNA cloning of Xenopus laevis skin peptidylhydroxyglycine N\u2010C lyase, catalyzing the second reaction of C\u2010terminal \u03b1\u2010amidation","display_name":"Purification and cDNA cloning of Xenopus laevis skin peptidylhydroxyglycine N\u2010C lyase, catalyzing the second reaction of C\u2010terminal \u03b1\u2010amidation","publication_year":1991,"publication_date":"1991-11-01","ids":{"openalex":"https://openalex.org/W2005341061","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb16314.x","mag":"2005341061","pmid":"https://pubmed.ncbi.nlm.nih.gov/1935950"},"language":"en","primary_location":{"id":"doi:10.1111/j.1432-1033.1991.tb16314.x","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb16314.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1991.tb16314.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111532860","display_name":"Yasuno Iwasaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405265712","display_name":"Ciba-Geigy Japan Limited (Japan)","ror":"https://ror.org/05h8ev729","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405265712"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasuno IWASAKI","raw_affiliation_strings":["Bio-organics Research Department, International Research Laboratories, Ciba-Geigy, Japan","Bio\u2010organics Research Department, Internatinal Research Laboratories, Ciba\u2010Geigy (Japan) Limited, Takarazuka, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bio-organics Research Department, International Research Laboratories, Ciba-Geigy, Japan","institution_ids":["https://openalex.org/I4405265712"]},{"raw_affiliation_string":"Bio\u2010organics Research Department, Internatinal Research Laboratories, Ciba\u2010Geigy (Japan) Limited, Takarazuka, Japan","institution_ids":["https://openalex.org/I4405265712"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Takashi KAWAHARA","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405265712","display_name":"Ciba-Geigy Japan Limited (Japan)","ror":"https://ror.org/05h8ev729","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405265712"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takashi KAWAHARA","raw_affiliation_strings":["Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan","institution_ids":["https://openalex.org/I4405265712"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041123057","display_name":"Hiroko SHIMOI","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405265712","display_name":"Ciba-Geigy Japan Limited (Japan)","ror":"https://ror.org/05h8ev729","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405265712"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroko SHIMOI","raw_affiliation_strings":["Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan","institution_ids":["https://openalex.org/I4405265712"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kenji SUZUKI","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405265712","display_name":"Ciba-Geigy Japan Limited (Japan)","ror":"https://ror.org/05h8ev729","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405265712"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenji SUZUKI","raw_affiliation_strings":["Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan","institution_ids":["https://openalex.org/I4405265712"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056087206","display_name":"Oreste Ghisalba","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Oreste GHISALBA","raw_affiliation_strings":["Central Research Laboratories, Ciba-Geigy AG, Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Laboratories, Ciba-Geigy AG, Basel, Switzerland","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068354355","display_name":"Kenji Kangawa","orcid":"https://orcid.org/0000-0003-4784-5599","observed_orcids":["https://orcid.org/0000-0003-4784-5599"]},"institutions":[{"id":"https://openalex.org/I118574687","display_name":"University of Miyazaki","ror":"https://ror.org/0447kww10","country_code":"JP","type":"education","lineage":["https://openalex.org/I118574687"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenji KANGAWA","raw_affiliation_strings":["Department of Biochemistry, Miyazaki Medical College, Kiyotake, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Miyazaki Medical College, Kiyotake, Japan","institution_ids":["https://openalex.org/I118574687"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103540959","display_name":"Hisayuki Matsuo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210111404","display_name":"National Cerebral and Cardiovascular Center","ror":"https://ror.org/01v55qb38","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210111404"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hisayuki MATSUO","raw_affiliation_strings":["National Cardiovascular Center Research Institute, Suita, Osaka, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Cardiovascular Center Research Institute, Suita, Osaka, Japan","institution_ids":["https://openalex.org/I4210111404"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100840467","display_name":"Yoshiki Nishikawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405265712","display_name":"Ciba-Geigy Japan Limited (Japan)","ror":"https://ror.org/05h8ev729","country_code":"JP","type":"company","lineage":["https://openalex.org/I4405265712"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Yoshiki NISHIKAWA","raw_affiliation_strings":["Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bio-organics Research Department, Internatinal Research Laboratories, Ciba-Geigy (Japan) Limited, Takarazuka, Japan","institution_ids":["https://openalex.org/I4405265712"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5100840467"],"corresponding_institution_ids":["https://openalex.org/I4405265712"],"apc_list":null,"apc_paid":null,"fwci":0.915,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.70364703,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"201","issue":"3","first_page":"551","last_page":"559"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.9779999852180481,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.9779999852180481,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11541","display_name":"Neuroendocrine regulation and behavior","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/3207","display_name":"Social Psychology"},"field":{"id":"https://openalex.org/fields/32","display_name":"Psychology"},"domain":{"id":"https://openalex.org/domains/2","display_name":"Social Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peptidylglycine-alpha-hydroxylating-monooxygenase","display_name":"peptidylglycine alpha-hydroxylating monooxygenase","score":0.9789999723434448},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6370000243186951},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.9980000257492065},{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"alternative splicing","score":0.7310000061988831},{"id":"https://openalex.org/keywords/protein-precursors","display_name":"precursor protein","score":0.5180000066757202},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.5070000290870667}],"concepts":[{"id":"https://openalex.org/C2779535977","wikidata":"https://www.wikidata.org/wiki/Q1342298","display_name":"Xenopus","level":3,"score":0.815707802772522},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.8123552799224854},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.583885908126831},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5704792737960815},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5592251420021057},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4966917634010315},{"id":"https://openalex.org/C14053116","wikidata":"https://www.wikidata.org/wiki/Q284398","display_name":"N-terminus","level":4,"score":0.46553435921669006},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.45068424940109253},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.4460087716579437},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.4312704801559448},{"id":"https://openalex.org/C57711820","wikidata":"https://www.wikidata.org/wiki/Q310425","display_name":"C-terminus","level":3,"score":0.42647719383239746},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.33622655272483826},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000577","descriptor_name":"Amides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002116","descriptor_name":"Calcitonin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004792","descriptor_name":"Enzyme Precursors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004792","descriptor_name":"Enzyme Precursors","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008190","descriptor_name":"Lyases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008190","descriptor_name":"Lyases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D008190","descriptor_name":"Lyases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012867","descriptor_name":"Skin","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D014982","descriptor_name":"Xenopus laevis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015139","descriptor_name":"Blotting, Southern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019760","descriptor_name":"Amidine-Lyases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1432-1033.1991.tb16314.x","doi":"https://doi.org/10.1111/j.1432-1033.1991.tb16314.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1432-1033.1991.tb16314.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:1935950","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1935950","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/15","score":0.4300000071525574,"display_name":"Life on land"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W227701090","https://openalex.org/W1489921776","https://openalex.org/W1515063683","https://openalex.org/W1534946518","https://openalex.org/W1552487687","https://openalex.org/W1963731302","https://openalex.org/W1995267671","https://openalex.org/W2007995076","https://openalex.org/W2025051667","https://openalex.org/W2027427161","https://openalex.org/W2050338422","https://openalex.org/W2062650520","https://openalex.org/W2063103311","https://openalex.org/W2067995683","https://openalex.org/W2091213702","https://openalex.org/W2092174485","https://openalex.org/W2095078947","https://openalex.org/W2100837269","https://openalex.org/W2128635872","https://openalex.org/W2138270253","https://openalex.org/W2144634347","https://openalex.org/W2159607115","https://openalex.org/W2314225431","https://openalex.org/W2346237422","https://openalex.org/W4255020332"],"related_works":["https://openalex.org/W2026187430","https://openalex.org/W2067995683","https://openalex.org/W1998294953","https://openalex.org/W2346237422","https://openalex.org/W2031243715","https://openalex.org/W1586989529","https://openalex.org/W227701090","https://openalex.org/W132696740","https://openalex.org/W7101205557","https://openalex.org/W4252693984"],"abstract_inverted_index":{"The":[0,70],"alpha-amidation":[1],"of":[2,86],"glycine-extended":[3],"peptides":[4],"is":[5,76,110],"a":[6,50],"two-step":[7],"reaction":[8],"catalyzed":[9],"by":[10,119,125],"peptidylglycine":[11],"alpha-hydroxylating":[12],"monooxygenase":[13],"(PHM)":[14],"and":[15,29,59,66,114,123],"peptidylhydroxyglycine":[16],"N-C":[17],"lyase":[18],"(PHL).":[19],"PHL":[20,60],"was":[21,40,43],"purified":[22],"to":[23,79],"homogeneity":[24],"from":[25,82],"Xenopus":[26],"laevis":[27,88],"skin":[28],"its":[30,63,106],"partial":[31],"amino":[32],"acid":[33],"sequence":[34,72,85],"(including":[35],"the":[36,46,57,83,97,120],"N-terminal":[37],"35":[38],"residues)":[39],"determined.":[41],"It":[42,109],"found":[44],"that":[45,80,96,112],"cDNA":[47],"codes":[48],"for":[49],"935-residue":[51],"precursor":[52],"protein":[53,75,99],"(AE-III":[54],"protein),":[55],"containing":[56],"PHM":[58,71],"sequences":[61],"at":[62,105],"N":[64],"terminus":[65],"C":[67,107],"terminus,":[68],"respectively.":[69],"in":[73],"AE-III":[74,115],"completely":[77],"identical":[78],"deduced":[81],"nucleotide":[84],"X.":[87],"AE-I":[89,98,113],"cDNA,":[90],"which":[91],"encodes":[92],"only":[93],"PHM,":[94],"except":[95],"has":[100],"an":[101],"extra":[102],"10":[103],"residues":[104],"terminus.":[108],"suggested":[111],"mRNA":[116],"are":[117],"encoded":[118],"same":[121],"gene":[122],"produced":[124],"alternative":[126],"splicing.":[127]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}