{"id":"https://openalex.org/W2005185423","doi":"https://doi.org/10.1021/bi00306a022","title":"Isolation of a heme-controlled inhibitor of translation that blocks the interaction between messenger RNA and eukaryotic initiation factor 2","display_name":"Isolation of a heme-controlled inhibitor of translation that blocks the interaction between messenger RNA and eukaryotic initiation factor 2","publication_year":1984,"publication_date":"1984-05-22","ids":{"openalex":"https://openalex.org/W2005185423","doi":"https://doi.org/10.1021/bi00306a022","mag":"2005185423","pmid":"https://pubmed.ncbi.nlm.nih.gov/6477877"},"language":"en","primary_location":{"id":"doi:10.1021/bi00306a022","doi":"https://doi.org/10.1021/bi00306a022","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00306a022","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005872583","display_name":"Sarah Knoller","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sarah Knoller","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5012505186","display_name":"Raymond O.R. Kaempfer","orcid":"https://orcid.org/0000-0002-5865-4619","observed_orcids":["https://orcid.org/0000-0002-5865-4619"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Raymond Kaempfer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.1428,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.38876156,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"11","first_page":"2462","last_page":"2469"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/eif2","display_name":"eIF2","score":0.9399999976158142},{"id":"https://openalex.org/keywords/mrna-binding","display_name":"mRNA binding","score":0.5299999713897705},{"id":"https://openalex.org/keywords/translation-initiation","display_name":"translation initiation","score":0.5329999923706055},{"id":"https://openalex.org/keywords/globin-mrna","display_name":"globin mRNA","score":0.7820000052452087},{"id":"https://openalex.org/keywords/2-aminopurine","display_name":"2-aminopurine","score":0.8140000104904175},{"id":"https://openalex.org/keywords/ternary-complex-formation","display_name":"ternary complex formation","score":0.6389999985694885}],"concepts":[{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.6662551164627075},{"id":"https://openalex.org/C97154109","wikidata":"https://www.wikidata.org/wiki/Q906570","display_name":"Eukaryotic initiation factor","level":5,"score":0.6296745538711548},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.588304877281189},{"id":"https://openalex.org/C62493599","wikidata":"https://www.wikidata.org/wiki/Q424137","display_name":"Initiation factor","level":5,"score":0.5575709939002991},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.553416907787323},{"id":"https://openalex.org/C4718897","wikidata":"https://www.wikidata.org/wiki/Q5408698","display_name":"Eukaryotic translation","level":5,"score":0.5424095392227173},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5067067742347717},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.495702862739563},{"id":"https://openalex.org/C163235415","wikidata":"https://www.wikidata.org/wiki/Q899814","display_name":"Second messenger system","level":3,"score":0.4822562336921692},{"id":"https://openalex.org/C162092063","wikidata":"https://www.wikidata.org/wiki/Q238406","display_name":"Five-prime cap","level":5,"score":0.471794068813324},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.43329405784606934},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.42204049229621887},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3400602340698242},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3025504946708679},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19547989964485168},{"id":"https://openalex.org/C21786251","wikidata":"https://www.wikidata.org/wiki/Q14886350","display_name":"RNA editing","level":4,"score":0.15351244807243347},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.14042147994041443},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09365716576576233}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006418","descriptor_name":"Heme","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006418","descriptor_name":"Heme","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006418","descriptor_name":"Heme","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010448","descriptor_name":"Peptide Initiation Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010448","descriptor_name":"Peptide Initiation Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010448","descriptor_name":"Peptide Initiation Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012156","descriptor_name":"Reticulocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012156","descriptor_name":"Reticulocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012156","descriptor_name":"Reticulocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D015852","descriptor_name":"Eukaryotic Initiation Factor-2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015852","descriptor_name":"Eukaryotic Initiation Factor-2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015852","descriptor_name":"Eukaryotic Initiation Factor-2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019892","descriptor_name":"eIF-2 Kinase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019892","descriptor_name":"eIF-2 Kinase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019892","descriptor_name":"eIF-2 Kinase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/bi00306a022","doi":"https://doi.org/10.1021/bi00306a022","is_oa":false,"landing_page_url":"https://doi.org/10.1021/bi00306a022","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:6477877","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6477877","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W103081912","https://openalex.org/W258376682","https://openalex.org/W1479744411","https://openalex.org/W1481908209","https://openalex.org/W1485792634","https://openalex.org/W1485899599","https://openalex.org/W1508904094","https://openalex.org/W1519918229","https://openalex.org/W1522423422","https://openalex.org/W1554335583","https://openalex.org/W1968719690","https://openalex.org/W1970529107","https://openalex.org/W1984156496","https://openalex.org/W1990344643","https://openalex.org/W1993415920","https://openalex.org/W1994714347","https://openalex.org/W1995273385","https://openalex.org/W1997142408","https://openalex.org/W2001580461","https://openalex.org/W2023276597","https://openalex.org/W2026729363","https://openalex.org/W2028154108","https://openalex.org/W2032547866","https://openalex.org/W2032918970","https://openalex.org/W2034639899","https://openalex.org/W2040533353","https://openalex.org/W2042191344","https://openalex.org/W2049822260","https://openalex.org/W2052740275","https://openalex.org/W2056940370","https://openalex.org/W2057635096","https://openalex.org/W2057871509","https://openalex.org/W2062417484","https://openalex.org/W2066342343","https://openalex.org/W2071285351","https://openalex.org/W2074235288","https://openalex.org/W2081071291","https://openalex.org/W2089113264","https://openalex.org/W2092253073","https://openalex.org/W2100837269","https://openalex.org/W2126200877","https://openalex.org/W2128635872","https://openalex.org/W2135104435","https://openalex.org/W2160896069","https://openalex.org/W2172401009","https://openalex.org/W2213378166","https://openalex.org/W2409840855"],"related_works":["https://openalex.org/W2064821101","https://openalex.org/W119101529","https://openalex.org/W2042449363","https://openalex.org/W2016803661","https://openalex.org/W1525224242","https://openalex.org/W2013415450","https://openalex.org/W4312093519","https://openalex.org/W2128091055","https://openalex.org/W2462604896","https://openalex.org/W4247599887"],"abstract_inverted_index":{"A":[0],"heme-controlled":[1,36,135],"inhibitor":[2,25,37,74,102,136,147,159,212],"of":[3,10,41,45,47,50,59,66,72,175,188,192,202,209],"translation":[4,76],"was":[5],"isolated":[6],"from":[7],"the":[8,119,146,173,186,190,217],"S-100":[9],"rabbit":[11],"reticulocytes":[12],"by":[13,52,61,199,206,224,236],"a":[14,115,130,179,229],"novel":[15],"procedure":[16],"including":[17],"chromatography":[18,151],"on":[19,75,107,232,238],"double-stranded":[20],"ribonucleic":[21],"acid":[22],"(dsRNA)-cellulose.":[23],"The":[24,70,101,158,211],"thus":[26,213],"purified":[27],"is":[28,77,105],"extremely":[29],"active":[30],"and":[31,43,63,89,110,169,221],"functionally":[32],"resembles":[33],"previously":[34,133],"studied":[35],"preparations":[38,139],"in":[39],"terms":[40],"kinetics":[42],"extent":[44],"inhibition":[46,51,60,65],"translation,":[48],"relief":[49,58],"eukaryotic":[53],"initiation":[54],"factor":[55],"2":[56,97],"(eIF-2),":[57],"2-aminopurine,":[62],"preferential":[64],"alpha-over":[67],"beta-globin":[68],"synthesis.":[69],"action":[71],"this":[73],"resistant":[78],"to":[79,90,177,215],"treatment":[80],"with":[81,181,226],"bacterial":[82],"alkaline":[83],"phosphatase,":[84],"micrococcal":[85],"nuclease,":[86],"or":[87,98,138],"trypsin":[88],"incubation":[91],"at":[92],"95":[93],"degrees":[94],"C,":[95],"pH":[96,99],"12.":[100],"not":[103,142,153,161,205,223],"only":[104],"retained":[106],"DEAE-cellulose,":[108],"phosphocellulose,":[109],"dsRNA-cellulose":[111,150],"but":[112,171,204],"also":[113],"exhibits":[114],"high":[116],"affinity":[117],"for":[118,228],"dye":[120],"Cibacron":[121],"Blue,":[122],"properties":[123],"that":[124,126,140],"suggest":[125],"it":[127],"may":[128],"be":[129,196],"protein.":[131],"Unlike":[132],"described":[134],"preparations,":[137],"did":[141],"pass":[143],"over":[144],"dsRNA-cellulose,":[145],"recovered":[148],"upon":[149],"does":[152,160],"exhibit":[154],"eIF-2":[155,176,203,220,227,239],"kinase":[156],"activity.":[157],"block":[162,216],"ternary":[163],"complex":[164,180],"formation":[165,191],"between":[166,219],"eIF-2,":[167],"methionyl-tRNAfMet,":[168],"GTP":[170],"inhibits":[172],"ability":[174],"form":[178],"labeled":[182],"globin":[183],"mRNA.":[184,210],"In":[185],"presence":[187],"inhibitor,":[189],"mRNA/eIF-2":[193],"complexes":[194],"can":[195],"restored":[197],"effectively":[198],"an":[200,207],"excess":[201,208],"appears":[214],"interaction":[218],"mRNA":[222,233],"competing":[225],"binding":[230],"site":[231],"but,":[234],"instead,":[235],"acting":[237],"itself.(ABSTRACT":[240],"TRUNCATED":[241],"AT":[242],"250":[243],"WORDS)":[244]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2025-10-10T00:00:00"}