{"id":"https://openalex.org/W2004992548","doi":"https://doi.org/10.1111/j.1467-7652.2005.00155.x","title":"The Lem2 gene promoter of barley directs cell\u2010 and development\u2010specific expression of gfp in transgenic plants","display_name":"The Lem2 gene promoter of barley directs cell\u2010 and development\u2010specific expression of gfp in transgenic plants","publication_year":2005,"publication_date":"2005-09-22","ids":{"openalex":"https://openalex.org/W2004992548","doi":"https://doi.org/10.1111/j.1467-7652.2005.00155.x","mag":"2004992548","pmid":"https://pubmed.ncbi.nlm.nih.gov/17177783"},"language":"en","primary_location":{"id":"doi:10.1111/j.1467-7652.2005.00155.x","doi":"https://doi.org/10.1111/j.1467-7652.2005.00155.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1467-7652.2005.00155.x","pdf_url":null,"source":{"id":"https://openalex.org/S36510773","display_name":"Plant Biotechnology Journal","issn_l":"1467-7644","issn":["1467-7644","1467-7652"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Biotechnology Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029985033","display_name":"Tilahun Abebe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119234918","display_name":"University of Northern Iowa","ror":"https://ror.org/02h4qpx12","country_code":"US","type":"education","lineage":["https://openalex.org/I119234918"]},{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tilahun Abebe","raw_affiliation_strings":["Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA","Present address: Department of Biology, University of Northern Iowa, Cedar Falls, IA 50614, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I135310074"]},{"raw_affiliation_string":"Present address: Department of Biology, University of Northern Iowa, Cedar Falls, IA 50614, USA","institution_ids":["https://openalex.org/I119234918"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112471383","display_name":"Ron Skadsen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ron Skadsen","raw_affiliation_strings":["USDA-ARS, Cereal Crops Research Unit, 501 Walnut St., Madison, WI 53726, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USDA-ARS, Cereal Crops Research Unit, 501 Walnut St., Madison, WI 53726, USA","institution_ids":["https://openalex.org/I1312222531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047764580","display_name":"Minesh Patel","orcid":"https://orcid.org/0000-0002-5793-7879","observed_orcids":["https://orcid.org/0000-0002-5793-7879"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Minesh Patel","raw_affiliation_strings":["Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085218579","display_name":"Heidi F. Kaeppler","orcid":"https://orcid.org/0000-0003-2814-7912","observed_orcids":["https://orcid.org/0000-0003-2814-7912"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Heidi Kaeppler","raw_affiliation_strings":["Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA","institution_ids":["https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5112471383"],"corresponding_institution_ids":["https://openalex.org/I1312222531"],"apc_list":{"value":4320,"currency":"USD","value_usd":4320},"apc_paid":null,"fwci":2.5046,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.88601585,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"4","issue":"1","first_page":"35","last_page":"44"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.5360999703407288,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.5360999703407288,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.17919999361038208,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.08720000088214874,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/barley","display_name":"barley","score":0.8240000009536743},{"id":"https://openalex.org/keywords/promoter-analysis","display_name":"promoter analysis","score":0.800000011920929},{"id":"https://openalex.org/keywords/tissue-specific-expression","display_name":"tissue-specific gene expression","score":0.7360000014305115},{"id":"https://openalex.org/keywords/cell-specific-expression","display_name":"cell-specific expression","score":0.6299999952316284},{"id":"https://openalex.org/keywords/gfp-reporter","display_name":"GFP reporter","score":0.8130000233650208},{"id":"https://openalex.org/keywords/lemmas","display_name":"lemmas","score":0.3449999988079071},{"id":"https://openalex.org/keywords/palea","display_name":"palea","score":0.9649999737739563},{"id":"https://openalex.org/keywords/epicarp","display_name":"epicarp","score":0.8109999895095825},{"id":"https://openalex.org/keywords/coleoptile","display_name":"coleoptile","score":0.75},{"id":"https://openalex.org/keywords/glumes","display_name":"glumes","score":0.6740000247955322},{"id":"https://openalex.org/keywords/promoter-methylation","display_name":"promoter methylation","score":0.6959999799728394},{"id":"https://openalex.org/keywords/salicylic-acid","display_name":"salicylic acid","score":0.6869999766349792},{"id":"https://openalex.org/keywords/fusarium-graminearum","display_name":"Fusarium graminearum","score":0.8059999942779541},{"id":"https://openalex.org/keywords/transgenic-barley","display_name":"transgenic barley","score":0.5320000052452087},{"id":"https://openalex.org/keywords/promoter-deletion","display_name":"promoter deletion","score":0.5270000100135803}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8655844926834106},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.7882134318351746},{"id":"https://openalex.org/C94893600","wikidata":"https://www.wikidata.org/wiki/Q572097","display_name":"Coleoptile","level":2,"score":0.6174777150154114},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5646122097969055},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5636427998542786},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5631837248802185},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4703056216239929},{"id":"https://openalex.org/C97037327","wikidata":"https://www.wikidata.org/wiki/Q5334291","display_name":"Ectopic expression","level":3,"score":0.4148215353488922},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3837107717990875},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.28413838148117065},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.16683095693588257}],"mesh":[{"descriptor_ui":"D001467","descriptor_name":"Hordeum","qualifier_ui":"Q000033","qualifier_name":"anatomy & histology","is_major_topic":false},{"descriptor_ui":"D001467","descriptor_name":"Hordeum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001467","descriptor_name":"Hordeum","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014170","descriptor_name":"Transformation, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017384","descriptor_name":"Sequence Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018506","descriptor_name":"Gene Expression Regulation, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018507","descriptor_name":"Gene Expression Regulation, Developmental","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D024506","descriptor_name":"5' Flanking Region","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1111/j.1467-7652.2005.00155.x","doi":"https://doi.org/10.1111/j.1467-7652.2005.00155.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1467-7652.2005.00155.x","pdf_url":null,"source":{"id":"https://openalex.org/S36510773","display_name":"Plant Biotechnology Journal","issn_l":"1467-7644","issn":["1467-7644","1467-7652"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Biotechnology Journal","raw_type":"journal-article"},{"id":"pmid:17177783","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17177783","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant biotechnology journal","raw_type":"Journal Article"},{"id":"pmh:oai:scholarworks.uni.edu:facpub-3871","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uni.edu/facpub/2870","pdf_url":null,"source":{"id":"https://openalex.org/S4377196489","display_name":"UNI ScholarWorks (University of Northern Iowa)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I119234918","host_organization_name":"University of Northern Iowa","host_organization_lineage":["https://openalex.org/I119234918"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Publications","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5666999816894531,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W54783221","https://openalex.org/W1536805151","https://openalex.org/W1597993741","https://openalex.org/W1967003522","https://openalex.org/W1982989214","https://openalex.org/W1988732894","https://openalex.org/W1992151575","https://openalex.org/W1992993879","https://openalex.org/W1996747425","https://openalex.org/W2012709143","https://openalex.org/W2022605927","https://openalex.org/W2034015100","https://openalex.org/W2039997181","https://openalex.org/W2060866824","https://openalex.org/W2069894620","https://openalex.org/W2082091267","https://openalex.org/W2090245513","https://openalex.org/W2096996931","https://openalex.org/W2097404077","https://openalex.org/W2098998842","https://openalex.org/W2108684914","https://openalex.org/W2125554759","https://openalex.org/W2126049819","https://openalex.org/W2134935936","https://openalex.org/W2135254089","https://openalex.org/W2162283732","https://openalex.org/W2164154631","https://openalex.org/W2168129156"],"related_works":["https://openalex.org/W1225897062","https://openalex.org/W2082049374","https://openalex.org/W1995324558","https://openalex.org/W2003864887","https://openalex.org/W2073588294","https://openalex.org/W2099888478","https://openalex.org/W2139751264","https://openalex.org/W2150384780","https://openalex.org/W2095180061","https://openalex.org/W2024295634"],"abstract_inverted_index":{"Transgenic":[0],"approaches":[1],"to":[2,67,104,198,204,224],"combating":[3],"fungal":[4],"pathogens,":[5],"such":[6],"as":[7],"Fusarium":[8],"graminearum,":[9],"require":[10],"the":[11,62,68,76,105,115,124,138,145,153,169,177,181,186,196,205,209,215,222,229],"targeting":[12],"of":[13,19,23,33,95,111,141,183,221,231],"antifungal":[14],"gene":[15,32],"expression":[16,57,94,102,106,150,194,207,238],"in":[17,50,108,123,137,152,161,164,176,180,193,208,214],"tissues":[18],"developing":[20],"seed":[21],"spikes":[22],"cereal":[24],"grains,":[25],"especially":[26],"lemmas":[27],"and":[28,46,54,92,129,144,236],"epicarps.":[29],"The":[30],"Lem2":[31,86,157,170],"barley":[34,81],"encodes":[35],"a":[36,190],"lectin-like":[37],"protein":[38,99],"that":[39,61,168],"is":[40,47,158,172],"strongly":[41],"up-regulated":[42],"by":[43],"salicylic":[44],"acid":[45],"preferentially":[48],"expressed":[49,160],"lemmas,":[51],"paleas":[52],"(lemma/palea)":[53],"coleoptiles.":[55],"Transient":[56],"studies":[58],"have":[59],"indicated":[60],"proximal":[63],"-75/+70":[64],"region":[65],"(relative":[66],"transcription":[69],"start":[70],"site)":[71],"determines":[72],"organ":[73,163],"specificity.":[74],"In":[75,185],"present":[77],"study,":[78],"Golden":[79],"Promise":[80],"stably":[82],"transformed":[83],"with":[84],"Morex":[85,112],"promoter/gfp":[87],"reporter":[88],"constructs":[89],"displayed":[90],"cell-":[91],"development-specific":[93],"gfp":[96,188,232],"(green":[97],"fluorescent":[98],"gene).":[100],"This":[101],"corresponded":[103],"seen":[107],"Northern":[109],"blots":[110],"organs.":[113],"Under":[114],"full-length":[116],"promoter,":[117],"strong":[118,149],"GFP":[119,132],"fluorescence":[120,133],"was":[121,134,212,239],"observed":[122,136],"lemma/palea,":[125,187,206],"glumes,":[126],"coleoptile,":[127],"auricle":[128],"ligule.":[130],"Weak":[131],"also":[135],"rachis,":[139],"tips":[140],"primary":[142],"leaves":[143],"leaf":[146,210],"sheath.":[147],"Unexpectedly,":[148],"occurred":[151],"epicarp,":[154],"even":[155],"though":[156],"not":[159],"this":[162],"Morex.":[165,184],"Studies":[166],"showed":[167],"promoter":[171,223],"more":[173],"highly":[174],"methylated":[175],"epicarp":[178],"than":[179],"lemma":[182],"underwent":[189],"temporal":[191],"shift":[192],"from":[195],"mesophyll":[197],"specialized":[199],"epidermal":[200],"cork":[201,216],"cells.":[202,217],"Similar":[203],"sheath":[211],"localized":[213],"Progressive":[218],"5'":[219],"deletions":[220],"nucleotide":[225],"-75":[226],"gradually":[227],"reduced":[228],"level":[230],"expression,":[233],"but":[234],"tissue-":[235],"cell-specific":[237],"retained.":[240]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}