{"id":"https://openalex.org/W2004709114","doi":"https://doi.org/10.1074/jbc.272.12.8090","title":"Cloning, Isolation, and Characterization of Mammalian Legumain, an Asparaginyl Endopeptidase","display_name":"Cloning, Isolation, and Characterization of Mammalian Legumain, an Asparaginyl Endopeptidase","publication_year":1997,"publication_date":"1997-03-01","ids":{"openalex":"https://openalex.org/W2004709114","doi":"https://doi.org/10.1074/jbc.272.12.8090","mag":"2004709114","pmid":"https://pubmed.ncbi.nlm.nih.gov/9065484"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.272.12.8090","doi":"https://doi.org/10.1074/jbc.272.12.8090","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.272.12.8090","pdf_url":"http://www.jbc.org/article/S0021925819675964/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925819675964/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049438802","display_name":"Jinq-May Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1302113299","display_name":"Babraham Institute","ror":"https://ror.org/01d5qpn59","country_code":"GB","type":"facility","lineage":["https://openalex.org/I1302113299","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jinq-May Chen","raw_affiliation_strings":["Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom","Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom","institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"]},{"raw_affiliation_string":"Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the","institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056445717","display_name":"Pam M. Dando","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1302113299","display_name":"Babraham Institute","ror":"https://ror.org/01d5qpn59","country_code":"GB","type":"facility","lineage":["https://openalex.org/I1302113299","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Pam M. Dando","raw_affiliation_strings":["Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the","institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051168121","display_name":"Neil David Rawlings","orcid":"https://orcid.org/0000-0001-5557-7665","observed_orcids":["https://orcid.org/0000-0001-5557-7665"]},"institutions":[{"id":"https://openalex.org/I1302113299","display_name":"Babraham Institute","ror":"https://ror.org/01d5qpn59","country_code":"GB","type":"facility","lineage":["https://openalex.org/I1302113299","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Neil D. Rawlings","raw_affiliation_strings":["Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the","institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015158694","display_name":"Molly A. Brown","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177639307","display_name":"University of Dundee","ror":"https://ror.org/03h2bxq36","country_code":"GB","type":"education","lineage":["https://openalex.org/I177639307"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Molly A. Brown","raw_affiliation_strings":["Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom","institution_ids":["https://openalex.org/I177639307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089549319","display_name":"Nina E. Young","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177639307","display_name":"University of Dundee","ror":"https://ror.org/03h2bxq36","country_code":"GB","type":"education","lineage":["https://openalex.org/I177639307"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Nina E. Young","raw_affiliation_strings":["Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom","institution_ids":["https://openalex.org/I177639307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111410898","display_name":"Richard Stevens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1302113299","display_name":"Babraham Institute","ror":"https://ror.org/01d5qpn59","country_code":"GB","type":"facility","lineage":["https://openalex.org/I1302113299","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Richard A. Stevens","raw_affiliation_strings":["Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the","institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072558369","display_name":"Eric W. Hewitt","orcid":"https://orcid.org/0000-0002-6238-6303","observed_orcids":["https://orcid.org/0000-0002-6238-6303"]},"institutions":[{"id":"https://openalex.org/I177639307","display_name":"University of Dundee","ror":"https://ror.org/03h2bxq36","country_code":"GB","type":"education","lineage":["https://openalex.org/I177639307"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Eric Hewitt","raw_affiliation_strings":["Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom","institution_ids":["https://openalex.org/I177639307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001667624","display_name":"Colin K. W. Watts","orcid":"https://orcid.org/0000-0001-6183-2087","observed_orcids":["https://orcid.org/0000-0001-6183-2087"]},"institutions":[{"id":"https://openalex.org/I177639307","display_name":"University of Dundee","ror":"https://ror.org/03h2bxq36","country_code":"GB","type":"education","lineage":["https://openalex.org/I177639307"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Colin Watts","raw_affiliation_strings":["Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, United Kingdom","institution_ids":["https://openalex.org/I177639307"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049066393","display_name":"A. John Barrett","orcid":"https://orcid.org/0000-0002-6082-4437","observed_orcids":["https://orcid.org/0000-0002-6082-4437"]},"institutions":[{"id":"https://openalex.org/I1302113299","display_name":"Babraham Institute","ror":"https://ror.org/01d5qpn59","country_code":"GB","type":"facility","lineage":["https://openalex.org/I1302113299","https://openalex.org/I2799693246","https://openalex.org/I4210087105"]},{"id":"https://openalex.org/I90344618","display_name":"Medical Research Council","ror":"https://ror.org/03x94j517","country_code":"GB","type":"government","lineage":["https://openalex.org/I4210087105","https://openalex.org/I90344618"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Alan J. Barrett","raw_affiliation_strings":["Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Research Council Peptidase Laboratory, Department of Immunology, The Babraham Institute, Babraham Hall, Babraham, Cambridgeshire CB2 4AT, United Kingdom and the","institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5049066393"],"corresponding_institution_ids":["https://openalex.org/I1302113299","https://openalex.org/I90344618"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.6799,"has_fulltext":true,"cited_by_count":386,"citation_normalized_percentile":{"value":0.94308438,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"272","issue":"12","first_page":"8090","last_page":"8098"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.975600004196167,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.975600004196167,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12505","display_name":"Peptidase Inhibition and Analysis","score":0.014999999664723873,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/legumain","display_name":"legumain","score":0.9990000128746033},{"id":"https://openalex.org/keywords/asparaginyl-endopeptidase","display_name":"asparaginyl endopeptidase","score":0.9890000224113464},{"id":"https://openalex.org/keywords/cysteine-endopeptidase","display_name":"cysteine endopeptidase","score":0.9570000171661377},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.6430000066757202},{"id":"https://openalex.org/keywords/enzyme-specificity","display_name":"enzyme specificity","score":0.5099999904632568},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.5509999990463257},{"id":"https://openalex.org/keywords/cystatin-c","display_name":"cystatin C","score":0.8450000286102295},{"id":"https://openalex.org/keywords/iodoacetamide","display_name":"iodoacetamide","score":0.7990000247955322},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.3880000114440918},{"id":"https://openalex.org/keywords/deglycosylation","display_name":"deglycosylation","score":0.7900000214576721},{"id":"https://openalex.org/keywords/schistosoma-mansoni","display_name":"Schistosoma mansoni","score":0.7429999709129333}],"concepts":[{"id":"https://openalex.org/C2777774944","wikidata":"https://www.wikidata.org/wiki/Q417262","display_name":"Endopeptidase","level":3,"score":0.8027896881103516},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7126381397247314},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.5731619000434875},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.531836748123169},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.49701955914497375},{"id":"https://openalex.org/C2776515853","wikidata":"https://www.wikidata.org/wiki/Q2191895","display_name":"Iodoacetamide","level":4,"score":0.4459477663040161},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.4151628017425537},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3740348219871521},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3462558686733246}],"mesh":[{"descriptor_ui":"D000099240","descriptor_name":"Asparaginyl Endopeptidase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003546","descriptor_name":"Cysteine Endopeptidases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003546","descriptor_name":"Cysteine Endopeptidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D003546","descriptor_name":"Cysteine Endopeptidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006868","descriptor_name":"Hydrolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015853","descriptor_name":"Cysteine Proteinase Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017208","descriptor_name":"Rats, Wistar","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1074/jbc.272.12.8090","doi":"https://doi.org/10.1074/jbc.272.12.8090","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.272.12.8090","pdf_url":"http://www.jbc.org/article/S0021925819675964/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:9065484","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9065484","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:discovery.dundee.ac.uk:openaire_cris_publications/1972da6b-8aeb-4641-86d2-117bc766dbfb","doi":null,"is_oa":false,"landing_page_url":"https://discovery.dundee.ac.uk/en/publications/1972da6b-8aeb-4641-86d2-117bc766dbfb","pdf_url":null,"source":{"id":"https://openalex.org/S4306400523","display_name":"Discovery Research Portal (University of Dundee)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I177639307","host_organization_name":"University of Dundee","host_organization_lineage":["https://openalex.org/I177639307"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Chen, J M, Dando, P M, Rawlings, N D, Brown, M A, Young, N E, Stevens, R A, Hewitt, E, Watts, C & Barrett, A J 1997, 'Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase', Journal of Biological Chemistry, vol. 272, no. 12, pp. 8090-8098. https://doi.org/10.1074/jbc.272.12.8090","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1074/jbc.272.12.8090","doi":"https://doi.org/10.1074/jbc.272.12.8090","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.272.12.8090","pdf_url":"http://www.jbc.org/article/S0021925819675964/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2004709114.pdf","grobid_xml":"https://content.openalex.org/works/W2004709114.grobid-xml"},"referenced_works_count":40,"referenced_works":["https://openalex.org/W282147426","https://openalex.org/W1484675846","https://openalex.org/W1504805361","https://openalex.org/W1546570611","https://openalex.org/W1546898680","https://openalex.org/W1555403344","https://openalex.org/W1572600466","https://openalex.org/W1600346161","https://openalex.org/W1812339273","https://openalex.org/W1889298235","https://openalex.org/W1939366980","https://openalex.org/W1967589959","https://openalex.org/W1975304761","https://openalex.org/W1985101779","https://openalex.org/W2006762876","https://openalex.org/W2010320204","https://openalex.org/W2019554080","https://openalex.org/W2022204091","https://openalex.org/W2023622367","https://openalex.org/W2027311860","https://openalex.org/W2029195137","https://openalex.org/W2036173317","https://openalex.org/W2036565421","https://openalex.org/W2045012663","https://openalex.org/W2048266624","https://openalex.org/W2053707285","https://openalex.org/W2071098547","https://openalex.org/W2072186058","https://openalex.org/W2087829285","https://openalex.org/W2091463834","https://openalex.org/W2091879499","https://openalex.org/W2102182903","https://openalex.org/W2108388593","https://openalex.org/W2128635872","https://openalex.org/W2138270253","https://openalex.org/W2200540262","https://openalex.org/W2341320514","https://openalex.org/W2344489676","https://openalex.org/W4238776363","https://openalex.org/W4301874489"],"related_works":["https://openalex.org/W4388325201","https://openalex.org/W2010730959","https://openalex.org/W1489746663","https://openalex.org/W1985619086","https://openalex.org/W2140636118","https://openalex.org/W2020527385","https://openalex.org/W2903059851","https://openalex.org/W2097912624","https://openalex.org/W2106896243","https://openalex.org/W2042011310"],"abstract_inverted_index":{"Legumain":[0],"is":[1,22,68,110,124,134,146,156,172],"a":[2,58,111,157,199,203],"cysteine":[3,29,158,200],"endopeptidase":[4,201],"that":[5,66],"shows":[6],"strict":[7],"specificity":[8,208],"for":[9],"hydrolysis":[10],"of":[11,31,81,113,195,198,202],"asparaginyl":[12,126],"bonds.":[13],"The":[14,97],"enzyme":[15,145],"belongs":[16],"to":[17,25,89,118],"peptidase":[18],"family":[19,205],"C13,":[20],"and":[21,39,57,75,79,130,143,152,163,181,206,211],"thus":[23],"unrelated":[24],"the":[26,32,144,193,196],"better":[27],"known":[28],"peptidases":[30],"papain":[33],"family,":[34],"C1":[35],"(Rawlings,":[36],"N.":[37],"D.,":[38],"Barrett,":[40],"A.":[41],"J.":[42],"(1994)":[43],"Methods":[44],"Enzymol.":[45],"244,":[46],"461-486).":[47],"To":[48],"date,":[49],"legumain":[50,67,78,88,109,155],"has":[51],"been":[52],"described":[53],"only":[54],"from":[55,94,105,177],"plants":[56],"blood":[59],"fluke,":[60],"Schistosoma":[61],"mansoni.":[62],"We":[63,72,84,191],"now":[64],"show":[65],"present":[69],"in":[70,209],"mammals.":[71,213],"have":[73,85],"cloned":[74],"sequenced":[76],"human":[77,182],"part":[80],"pig":[82,95],"legumain.":[83],"also":[86],"purified":[87],"homogeneity":[90],"(2200-fold,":[91],"8%":[92],"yield)":[93],"kidney.":[96],"mammalian":[98],"sequences":[99],"are":[100],"clearly":[101],"homologous":[102],"with":[103,185],"legumains":[104],"non-mammalian":[106],"species.":[107],"Pig":[108],"glycoprotein":[112],"about":[114],"34":[115],"kDa,":[116],"decreasing":[117],"31":[119],"kDa":[120],"on":[121],"deglycosylation.":[122],"It":[123,171],"an":[125],"endopeptidase,":[127,159],"hydrolyzing":[128],"Z-Ala-Ala-Asn-7-(4-methyl)coumarylamide":[129],"benzoyl-Asn-p-nitroanilide.":[131],"Maximal":[132],"activity":[133],"seen":[135],"at":[136,149],"pH":[137,150],"5.8":[138],"under":[139],"normal":[140],"assay":[141],"conditions,":[142],"irreversibly":[147],"denatured":[148],"7":[151],"above.":[153],"Mammalian":[154],"inhibited":[160,173],"by":[161,167,174],"iodoacetamide":[162],"maleimides,":[164],"but":[165],"unaffected":[166],"compound":[168],"E64":[169],"(trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane).":[170],"ovocystatin":[175],"(cystatin":[176],"chicken":[178],"egg":[179],"white)":[180],"cystatin":[183],"C":[184],"Ki":[186],"values":[187],"<":[188],"5":[189],"nM.":[190],"discuss":[192],"significance":[194],"discovery":[197],"new":[204],"distinctive":[207],"man":[210],"other":[212]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":15},{"year":2024,"cited_by_count":13},{"year":2023,"cited_by_count":18},{"year":2022,"cited_by_count":15},{"year":2021,"cited_by_count":19},{"year":2020,"cited_by_count":17},{"year":2019,"cited_by_count":14},{"year":2018,"cited_by_count":10},{"year":2017,"cited_by_count":21},{"year":2016,"cited_by_count":19},{"year":2015,"cited_by_count":16},{"year":2014,"cited_by_count":18},{"year":2013,"cited_by_count":18},{"year":2012,"cited_by_count":12}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}