{"id":"https://openalex.org/W2004374675","doi":"https://doi.org/10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","title":"An expression system matures: A highly efficient and cost-effective process for phytase production by recombinant strains ofHansenula polymorpha","display_name":"An expression system matures: A highly efficient and cost-effective process for phytase production by recombinant strains ofHansenula polymorpha","publication_year":1999,"publication_date":"1999-05-05","ids":{"openalex":"https://openalex.org/W2004374675","doi":"https://doi.org/10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","mag":"2004374675","pmid":"https://pubmed.ncbi.nlm.nih.gov/10099617"},"language":"en","primary_location":{"id":"doi:10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","doi":"https://doi.org/10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009416151","display_name":"Anne F. Mayer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"Roche (Switzerland)","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"A. F. Mayer","raw_affiliation_strings":["Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]},{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082256196","display_name":"Karsten Hellmuth","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"Roche (Switzerland)","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"K. Hellmuth","raw_affiliation_strings":["Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]},{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064853121","display_name":"Heinrich Schlieker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"Roche (Switzerland)","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"H. Schlieker","raw_affiliation_strings":["Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]},{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034098212","display_name":"Rual Lopez\u2010Ulibarri","orcid":"https://orcid.org/0000-0002-7839-384X","observed_orcids":["https://orcid.org/0000-0002-7839-384X"]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"Roche (Switzerland)","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"R. Lopez-Ulibarri","raw_affiliation_strings":["Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]},{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071925822","display_name":"Stefan Oertel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"Roche (Switzerland)","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"S. Oertel","raw_affiliation_strings":["Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]},{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045996313","display_name":"Ulrike M. Dahlems","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"U. Dahlems","raw_affiliation_strings":["Rhein Biotech GmbH, Eichsfelder Strasse 11, D-40595 Du\u0308sseldorf, Germany","Rhein Biotech GmbH, Eichsfelder Strasse 11, D\u201040595 D\u00fcsseldorf, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rhein Biotech GmbH, Eichsfelder Strasse 11, D-40595 Du\u0308sseldorf, Germany","institution_ids":[]},{"raw_affiliation_string":"Rhein Biotech GmbH, Eichsfelder Strasse 11, D\u201040595 D\u00fcsseldorf, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046532595","display_name":"Andreas Strasser","orcid":"https://orcid.org/0000-0002-5020-4891","observed_orcids":["https://orcid.org/0000-0002-5020-4891"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. W. M. Strasser","raw_affiliation_strings":["Rhein Biotech GmbH, Eichsfelder Strasse 11, D-40595 Du\u0308sseldorf, Germany","Rhein Biotech GmbH, Eichsfelder Strasse 11, D\u201040595 D\u00fcsseldorf, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rhein Biotech GmbH, Eichsfelder Strasse 11, D-40595 Du\u0308sseldorf, Germany","institution_ids":[]},{"raw_affiliation_string":"Rhein Biotech GmbH, Eichsfelder Strasse 11, D\u201040595 D\u00fcsseldorf, Germany","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108408556","display_name":"Adolphus P. G. M. van Loon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"Roche (Switzerland)","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":true,"raw_author_name":"A. P. G. M. van Loon","raw_affiliation_strings":["Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann-La Roche AG, Grenzacherstrasse 124, CH-4070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]},{"raw_affiliation_string":"Biotechnology Research Group, Vitamins and Fine Chemicals Division, F. Hoffmann\u2010La Roche AG, Grenzacherstrasse 124, CH\u20104070 Basel, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5108408556"],"corresponding_institution_ids":["https://openalex.org/I118019719"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":10.1281,"has_fulltext":false,"cited_by_count":148,"citation_normalized_percentile":{"value":0.98035896,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"63","issue":"3","first_page":"373","last_page":"381"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phytase-production","display_name":"phytase production","score":0.9679999947547913},{"id":"https://openalex.org/keywords/hansenula-polymorpha","display_name":"Hansenula polymorpha","score":0.9990000128746033},{"id":"https://openalex.org/keywords/glucose-as-carbon-source","display_name":"glucose carbon source","score":0.8579999804496765},{"id":"https://openalex.org/keywords/glycerol","display_name":"glycerol","score":0.6480000019073486},{"id":"https://openalex.org/keywords/fermentation-optimization","display_name":"fermentation optimization","score":0.5720000267028809},{"id":"https://openalex.org/keywords/downstream-processing","display_name":"downstream processing","score":0.7070000171661377},{"id":"https://openalex.org/keywords/product-recovery","display_name":"product recovery","score":0.675000011920929},{"id":"https://openalex.org/keywords/pilot-scale-fermentation","display_name":"pilot-scale fermentation","score":0.7860000133514404}],"concepts":[{"id":"https://openalex.org/C109551439","wikidata":"https://www.wikidata.org/wiki/Q410099","display_name":"Phytase","level":3,"score":0.9304141402244568},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.6917251348495483},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.641242504119873},{"id":"https://openalex.org/C206139338","wikidata":"https://www.wikidata.org/wiki/Q192355","display_name":"Raw material","level":2,"score":0.5548315644264221},{"id":"https://openalex.org/C2780881558","wikidata":"https://www.wikidata.org/wiki/Q132501","display_name":"Glycerol","level":2,"score":0.5156994462013245},{"id":"https://openalex.org/C67568554","wikidata":"https://www.wikidata.org/wiki/Q385442","display_name":"Downstream processing","level":2,"score":0.5044025182723999},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.4973485767841339},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.45453929901123047},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44988375902175903},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.42495957016944885},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38157665729522705},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3616870641708374},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.31260401010513306},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001232","descriptor_name":"Aspergillus fumigatus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001232","descriptor_name":"Aspergillus fumigatus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001232","descriptor_name":"Aspergillus fumigatus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001232","descriptor_name":"Aspergillus fumigatus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001232","descriptor_name":"Aspergillus fumigatus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001232","descriptor_name":"Aspergillus fumigatus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000191","qualifier_name":"economics","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000191","qualifier_name":"economics","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000191","qualifier_name":"economics","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003362","descriptor_name":"Cost-Benefit Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003362","descriptor_name":"Cost-Benefit Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003362","descriptor_name":"Cost-Benefit Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005990","descriptor_name":"Glycerol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005990","descriptor_name":"Glycerol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005990","descriptor_name":"Glycerol","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010832","descriptor_name":"6-Phytase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D018533","descriptor_name":"Biomass","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018533","descriptor_name":"Biomass","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018533","descriptor_name":"Biomass","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","doi":"https://doi.org/10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","is_oa":false,"landing_page_url":"https://doi.org/10.1002/(sici)1097-0290(19990505)63:3<373::aid-bit14>3.0.co;2-t","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:10099617","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10099617","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8799999952316284,"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12"},{"score":0.4359000027179718,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"sustainable_development_goals_aurora":[{"score":0.41999998688697815,"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1526043578","https://openalex.org/W1586727832","https://openalex.org/W1600651336","https://openalex.org/W1965020882","https://openalex.org/W1978654705","https://openalex.org/W1984746385","https://openalex.org/W2015020062","https://openalex.org/W2015624272","https://openalex.org/W2019410656","https://openalex.org/W2025037117","https://openalex.org/W2034194918","https://openalex.org/W2034309871","https://openalex.org/W2045344155","https://openalex.org/W2059451237","https://openalex.org/W2082403691","https://openalex.org/W2099639154","https://openalex.org/W2100035052","https://openalex.org/W2102501687","https://openalex.org/W2168848023","https://openalex.org/W2405819545","https://openalex.org/W2463267938","https://openalex.org/W2497441547","https://openalex.org/W4211083240","https://openalex.org/W4296765338"],"related_works":["https://openalex.org/W2133748879","https://openalex.org/W2360362098","https://openalex.org/W2272425703","https://openalex.org/W1993893577","https://openalex.org/W2371008086","https://openalex.org/W2210012445","https://openalex.org/W157492138","https://openalex.org/W2767857927","https://openalex.org/W4200207694","https://openalex.org/W4242630983"],"abstract_inverted_index":{"An":[0],"efficient":[1,117],"process":[2,46,56],"was":[3,32],"developed":[4,122],"for":[5,38,54,111,158],"the":[6,34,45,49,59,72,91,99,130],"low-cost":[7,159],"production":[8,40],"of":[9,41,61,67,81,98],"phytases":[10],"using":[11,47],"Hansenula":[12],"polymorpha.":[13],"Glucose":[14,31],"or":[15],"glucose":[16,62],"syrups,":[17],"previously":[18],"reported":[19,108],"as":[20,25,153],"repressive":[21],"substrates,":[22],"were":[23,88,121,135],"used":[24,53,152],"main":[26],"carbon":[27,51],"sources":[28],"during":[29],"fermentation.":[30],"even":[33],"most":[35],"productive":[36],"substrate":[37],"high-level":[39],"phytases.":[42],"Compared":[43],"with":[44,93,123],"glycerol,":[48],"standard":[50],"source":[52],"this":[55],"until":[57],"now,":[58],"use":[60],"led":[63],"to":[64,85,142],"a":[65,146,154],"reduction":[66],"more":[68],"than":[69],"80%":[70],"in":[71,90,138],"raw":[73],"materials":[74],"costs.":[75],"In":[76],"addition,":[77],"exceptionally":[78],"high":[79],"concentrations":[80],"active":[82],"enzyme":[83],"(up":[84],"13.5":[86],"g/L)":[87],"obtained":[89],"medium,":[92],"phytase":[94,160],"representing":[95],"over":[96,127],"97%":[97],"total":[100],"accumulated":[101],"protein.":[102],"These":[103],"levels":[104],"greatly":[105],"exceed":[106],"those":[107],"so":[109],"far":[110],"any":[112],"yeast-based":[113],"expression":[114],"system.":[115],"Very":[116],"downstream":[118,133],"processing":[119,134],"procedures":[120],"product":[124],"recovery":[125],"yields":[126],"90%.":[128],"Both":[129],"fermentation":[131],"and":[132],"successfully":[136],"tested":[137],"pilot":[139],"scale":[140],"up":[141],"2000":[143],"L.":[144],"As":[145],"result,":[147],"H.":[148],"polymorpha":[149],"can":[150],"be":[151],"highly":[155],"competitive":[156],"system":[157],"production.":[161]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":8},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}