{"id":"https://openalex.org/W2004335062","doi":"https://doi.org/10.1073/pnas.86.8.2592","title":"Native and mutant 5-lipoxygenase expression in a baculovirus/insect cell system.","display_name":"Native and mutant 5-lipoxygenase expression in a baculovirus/insect cell system.","publication_year":1989,"publication_date":"1989-04-01","ids":{"openalex":"https://openalex.org/W2004335062","doi":"https://doi.org/10.1073/pnas.86.8.2592","mag":"2004335062","pmid":"https://pubmed.ncbi.nlm.nih.gov/2649885","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/286963"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.86.8.2592","doi":"https://doi.org/10.1073/pnas.86.8.2592","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.86.8.2592","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC286963","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032705392","display_name":"Colin D. Funk","orcid":"https://orcid.org/0000-0001-7029-4233","observed_orcids":["https://orcid.org/0000-0001-7029-4233"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"C D Funk","raw_affiliation_strings":["Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051603564","display_name":"Hans Gunne","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"H Gunne","raw_affiliation_strings":["Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111648753","display_name":"H. Steiner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"H Steiner","raw_affiliation_strings":["Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101563826","display_name":"Takashi Izumi","orcid":"https://orcid.org/0000-0002-4268-1874","observed_orcids":["https://orcid.org/0000-0002-4268-1874"]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"T Izumi","raw_affiliation_strings":["Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]},{"author_position":"last","author":{"id":null,"display_name":"B Samuelsson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28166907","display_name":"Karolinska Institutet","ror":"https://ror.org/056d84691","country_code":"SE","type":"education","lineage":["https://openalex.org/I28166907"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"B Samuelsson","raw_affiliation_strings":["Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden","institution_ids":["https://openalex.org/I28166907"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I28166907"],"apc_list":null,"apc_paid":null,"fwci":2.9075,"has_fulltext":false,"cited_by_count":61,"citation_normalized_percentile":{"value":0.92813735,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"86","issue":"8","first_page":"2592","last_page":"2596"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.8804000020027161,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.8804000020027161,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12910","display_name":"Eicosanoids and Hypertension Pharmacology","score":0.022199999541044235,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.019600000232458115,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/5-lipoxygenase","display_name":"5-lipoxygenase","score":0.9900000095367432},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8659999966621399},{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.9419999718666077},{"id":"https://openalex.org/keywords/spodoptera-frugiperda-cells","display_name":"Spodoptera frugiperda cells","score":0.9210000038146973},{"id":"https://openalex.org/keywords/leukotriene-biosynthesis","display_name":"leukotriene biosynthesis","score":0.7179999947547913},{"id":"https://openalex.org/keywords/arachidonic-acid-metabolism","display_name":"arachidonic acid metabolism","score":0.7820000052452087},{"id":"https://openalex.org/keywords/histidine-mutations","display_name":"histidine mutations","score":0.8050000071525574},{"id":"https://openalex.org/keywords/recombinant-baculovirus","display_name":"recombinant baculovirus","score":0.722000002861023},{"id":"https://openalex.org/keywords/insect-cell-expression-system","display_name":"insect cell expression","score":0.41999998688697815}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.8033078908920288},{"id":"https://openalex.org/C103878061","wikidata":"https://www.wikidata.org/wiki/Q28840674","display_name":"Sf9","level":5,"score":0.7267613410949707},{"id":"https://openalex.org/C2777773057","wikidata":"https://www.wikidata.org/wiki/Q2492887","display_name":"Spodoptera","level":4,"score":0.7191329002380371},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6370978355407715},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6154941916465759},{"id":"https://openalex.org/C2781140520","wikidata":"https://www.wikidata.org/wiki/Q24771294","display_name":"Polyhedrin","level":5,"score":0.5115509629249573},{"id":"https://openalex.org/C90342290","wikidata":"https://www.wikidata.org/wiki/Q94234745","display_name":"Arachidonate 5-lipoxygenase","level":4,"score":0.48415762186050415},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.48389872908592224},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4584767818450928},{"id":"https://openalex.org/C2780857362","wikidata":"https://www.wikidata.org/wiki/Q1827455","display_name":"Lipoxygenase","level":3,"score":0.45262759923934937},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.44367268681526184},{"id":"https://openalex.org/C2778460671","wikidata":"https://www.wikidata.org/wiki/Q485277","display_name":"Histidine","level":3,"score":0.4432612657546997},{"id":"https://openalex.org/C2778078955","wikidata":"https://www.wikidata.org/wiki/Q407699","display_name":"Arachidonic acid","level":3,"score":0.33523714542388916},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15111523866653442}],"mesh":[{"descriptor_ui":"D001091","descriptor_name":"Arachidonate Lipoxygenases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001094","descriptor_name":"Arachidonate 5-Lipoxygenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006639","descriptor_name":"Histidine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007304","descriptor_name":"Insect Viruses","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007501","descriptor_name":"Iron","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1073/pnas.86.8.2592","doi":"https://doi.org/10.1073/pnas.86.8.2592","is_oa":false,"landing_page_url":"https://doi.org/10.1073/pnas.86.8.2592","pdf_url":null,"source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:238477","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC286963","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:2649885","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2649885","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:286963","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC286963/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:238477","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC286963","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W1521454905","https://openalex.org/W1597733532","https://openalex.org/W1668867309","https://openalex.org/W1966164413","https://openalex.org/W1978342320","https://openalex.org/W1994862373","https://openalex.org/W2000662927","https://openalex.org/W2004351423","https://openalex.org/W2007082033","https://openalex.org/W2022750428","https://openalex.org/W2024176341","https://openalex.org/W2026913755","https://openalex.org/W2042869569","https://openalex.org/W2051183422","https://openalex.org/W2054044627","https://openalex.org/W2062300097","https://openalex.org/W2069111810","https://openalex.org/W2076704773","https://openalex.org/W2089073520","https://openalex.org/W2091448894","https://openalex.org/W2128635872","https://openalex.org/W2134624087","https://openalex.org/W2134812217","https://openalex.org/W2138270253","https://openalex.org/W2170293208","https://openalex.org/W2402672470","https://openalex.org/W2419338956","https://openalex.org/W2468468885"],"related_works":["https://openalex.org/W2042992963","https://openalex.org/W2003376770","https://openalex.org/W2044021413","https://openalex.org/W2065297082","https://openalex.org/W2033603399","https://openalex.org/W2092939650","https://openalex.org/W1974961079","https://openalex.org/W1648127659","https://openalex.org/W1521454905","https://openalex.org/W1832399072"],"abstract_inverted_index":{"Human":[0],"5-lipoxygenase":[1,38,74,134,193],"(EC":[2],"1.13.11.34),":[3],"the":[4,9,15,36,43,60,95,126,175,202,212],"key":[5],"enzyme":[6,65,195],"involved":[7],"in":[8,23,75,136,144,150,208],"transformation":[10],"of":[11,42,55,87,94,99,115,128,205],"arachidonic":[12],"acid":[13],"to":[14,83,141,169,181,190],"potent":[16],"biologically":[17],"active":[18],"leukotrienes,":[19],"has":[20],"been":[21],"overexpressed":[22],"insect":[24,183],"cells":[25,58,124,138,187],"using":[26],"a":[27,104,153,163],"baculovirus":[28,33],"expression":[29],"system.":[30],"A":[31],"recombinant":[32,61,73,122],"(3B6)":[34],"carrying":[35,174],"human":[37,119],"coding":[39],"sequence":[40],"downstream":[41],"strong":[44],"polyhedrin":[45],"protein":[46,68,135],"promoter":[47],"was":[48,81,139,197],"isolated.":[49],"Approximately":[50],"48":[51],"hr":[52],"after":[53],"infection":[54],"Spodoptera":[56],"frugiperda":[57],"with":[59,91],"baculovirus,":[62],"maximal":[63,111],"intracellular":[64],"activity":[66,196],"and":[67,102,108,121,157,179],"levels":[69],"were":[70,167,177,188],"detected.":[71],"The":[72],"10,000":[76],"x":[77],"g":[78],"supernatant":[79,116],"fractions":[80],"able":[82,189],"synthesize":[84],"large":[85],"amounts":[86,93,145],"5-hydroperoxy-6,8,10,14-icosatetraenoic":[88],"acid,":[89],"together":[90],"smaller":[92],"nonenzymatic":[96],"hydrolysis":[97],"products":[98],"leukotriene":[100],"A4,":[101],"exhibited":[103],"dependence":[105],"on":[106,152],"Ca2+":[107],"ATP":[109],"for":[110],"activity.":[112],"Immunoblot":[113],"analysis":[114],"proteins":[117],"from":[118],"leukocytes":[120,151],"virus-infected":[123],"indicated":[125],"presence":[127],"indistinguishable":[129],"approximately":[130],"80-kDa":[131],"bands.":[132],"However,":[133],"recombinant-infected":[137],"found":[140],"be":[142],"present":[143,149],"50-200":[146],"times":[147],"that":[148],"per-cell":[154],"basis.":[155],"Histidine-362":[156],"histidine-372,":[158],"potential":[159],"iron-atom":[160],"ligands":[161],"within":[162],"putative":[164,213],"iron-binding":[165],"domain,":[166],"changed":[168],"serine":[170],"residues.":[171],"Recombinant":[172],"baculoviruses":[173],"mutations":[176],"isolated":[178],"used":[180],"infect":[182],"cells.":[184],"Although":[185],"infected":[186],"express":[191],"mutant":[192],"protein,":[194],"not":[198],"substantially":[199],"altered,":[200],"suggesting":[201],"nonessential":[203],"nature":[204],"these":[206],"histidines":[207],"binding":[209],"iron":[210],"at":[211],"ferric":[214],"catalytic":[215],"site.":[216]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}