{"id":"https://openalex.org/W2004139080","doi":"https://doi.org/10.1016/s0300-9084(01)01341-4","title":"Chemical mechanism of \u03b2-xylosidase from Trichoderma reesei QM 9414: pH-dependence of kinetic parameters","display_name":"Chemical mechanism of \u03b2-xylosidase from Trichoderma reesei QM 9414: pH-dependence of kinetic parameters","publication_year":2001,"publication_date":"2001-10-01","ids":{"openalex":"https://openalex.org/W2004139080","doi":"https://doi.org/10.1016/s0300-9084(01)01341-4","mag":"2004139080","pmid":"https://pubmed.ncbi.nlm.nih.gov/11728634"},"language":"en","primary_location":{"id":"doi:10.1016/s0300-9084(01)01341-4","doi":"https://doi.org/10.1016/s0300-9084(01)01341-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0300-9084(01)01341-4","pdf_url":null,"source":{"id":"https://openalex.org/S75247133","display_name":"Biochimie","issn_l":"0037-9042","issn":["0037-9042","0300-9084","1638-6183"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimie","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089371617","display_name":"Mar\u00eda G\u00f3mez\u2010Ru\u00edz","orcid":"https://orcid.org/0000-0002-0295-8285","observed_orcids":["https://orcid.org/0000-0002-0295-8285"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Mar\u00eda G\u00f3mez","raw_affiliation_strings":["Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular I, Facultad de Biolog\u00eda, Universidad Complutense, 28040 Madrid, Spain","Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular I, Facultad de Biolog\u00eda, Universidad Complutense, 28040 Madrid, Spain","institution_ids":["https://openalex.org/I121748325"]},{"raw_affiliation_string":"Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034491931","display_name":"Pablo Isorna","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Pablo Isorna","raw_affiliation_strings":["Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078474851","display_name":"Marta Rojo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Marta Rojo","raw_affiliation_strings":["Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain","institution_ids":["https://openalex.org/I121748325"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101973939","display_name":"Pilar Estrada","orcid":"https://orcid.org/0000-0002-8376-6815","observed_orcids":["https://orcid.org/0000-0002-8376-6815"]},"institutions":[{"id":"https://openalex.org/I121748325","display_name":"Universidad Complutense de Madrid","ror":"https://ror.org/02p0gd045","country_code":"ES","type":"education","lineage":["https://openalex.org/I121748325"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Pilar Estrada","raw_affiliation_strings":["Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Bioquimica y Biologia Molecular I, Facultad de Biologia, Universidad Complutense, 28040 Madrid, Spain","institution_ids":["https://openalex.org/I121748325"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I121748325"],"apc_list":{"value":3110,"currency":"USD","value_usd":3110},"apc_paid":null,"fwci":0.2876,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.54863709,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"83","issue":"10","first_page":"961","last_page":"967"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.49410000443458557,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.49410000443458557,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.20419999957084656,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.0835999995470047,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-xylosidase","display_name":"\u03b2-xylosidase","score":0.9279999732971191},{"id":"https://openalex.org/keywords/trichoderma-reesei","display_name":"Trichoderma reesei","score":0.9610000252723694},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.7409999966621399},{"id":"https://openalex.org/keywords/carboxyl-groups","display_name":"carboxyl groups","score":0.652999997138977},{"id":"https://openalex.org/keywords/ionization-enthalpy","display_name":"ionization enthalpy","score":0.7839999794960022},{"id":"https://openalex.org/keywords/solvent-perturbation","display_name":"solvent perturbation","score":0.640999972820282},{"id":"https://openalex.org/keywords/substrate-protection","display_name":"substrate protection","score":0.4050000011920929}],"concepts":[{"id":"https://openalex.org/C2776125036","wikidata":"https://www.wikidata.org/wiki/Q2749932","display_name":"Trichoderma reesei","level":4,"score":0.8710838556289673},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8370700478553772},{"id":"https://openalex.org/C30095370","wikidata":"https://www.wikidata.org/wiki/Q899488","display_name":"Protonation","level":3,"score":0.7482094764709473},{"id":"https://openalex.org/C118629725","wikidata":"https://www.wikidata.org/wiki/Q2686738","display_name":"Deprotonation","level":3,"score":0.627827525138855},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.5314338207244873},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.5196551084518433},{"id":"https://openalex.org/C2780388412","wikidata":"https://www.wikidata.org/wiki/Q407569","display_name":"Gluconic acid","level":2,"score":0.5192520618438721},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.5183821320533752},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5174239873886108},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5164432525634766},{"id":"https://openalex.org/C155647269","wikidata":"https://www.wikidata.org/wiki/Q243455","display_name":"Medicinal chemistry","level":1,"score":0.38951411843299866},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.3540589213371277},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.20748558640480042},{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.06378933787345886},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.0},{"id":"https://openalex.org/C145148216","wikidata":"https://www.wikidata.org/wiki/Q36496","display_name":"Ion","level":2,"score":0.0},{"id":"https://openalex.org/C111368507","wikidata":"https://www.wikidata.org/wiki/Q43518","display_name":"Oceanography","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005942","descriptor_name":"Gluconates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006027","descriptor_name":"Glycosides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007783","descriptor_name":"Lactones","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014242","descriptor_name":"Trichoderma","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D014995","descriptor_name":"Xylosidases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D014995","descriptor_name":"Xylosidases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014995","descriptor_name":"Xylosidases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0300-9084(01)01341-4","doi":"https://doi.org/10.1016/s0300-9084(01)01341-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0300-9084(01)01341-4","pdf_url":null,"source":{"id":"https://openalex.org/S75247133","display_name":"Biochimie","issn_l":"0037-9042","issn":["0037-9042","0300-9084","1638-6183"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimie","raw_type":"journal-article"},{"id":"pmid:11728634","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11728634","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochimie","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W955725593","https://openalex.org/W1248348299","https://openalex.org/W1490515102","https://openalex.org/W1529041827","https://openalex.org/W1574246355","https://openalex.org/W1772341111","https://openalex.org/W1775749144","https://openalex.org/W1830745998","https://openalex.org/W1973898778","https://openalex.org/W1976860525","https://openalex.org/W2000500498","https://openalex.org/W2000600937","https://openalex.org/W2001352687","https://openalex.org/W2020091577","https://openalex.org/W2034421756","https://openalex.org/W2050723215","https://openalex.org/W2051696097","https://openalex.org/W2071098547","https://openalex.org/W2073869122","https://openalex.org/W2106121486","https://openalex.org/W2117899278","https://openalex.org/W2142111674","https://openalex.org/W2147705013","https://openalex.org/W2239034314","https://openalex.org/W2426131081","https://openalex.org/W2950469006","https://openalex.org/W4301874489","https://openalex.org/W6631739128","https://openalex.org/W6668501022"],"related_works":["https://openalex.org/W2239034314","https://openalex.org/W1974384307","https://openalex.org/W1968165104","https://openalex.org/W2017880409","https://openalex.org/W2398200165","https://openalex.org/W2015150958","https://openalex.org/W1138901821","https://openalex.org/W2006582015","https://openalex.org/W2000600937","https://openalex.org/W2064142837"],"abstract_inverted_index":null,"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}