{"id":"https://openalex.org/W2003449946","doi":"https://doi.org/10.1021/ac8016407","title":"Characterization of a Multiple Ligand-Gated Ion Channel Cellular Membrane Affinity Chromatography Column and Identification of Endogenously Expressed Receptors in Astrocytoma Cell Lines","display_name":"Characterization of a Multiple Ligand-Gated Ion Channel Cellular Membrane Affinity Chromatography Column and Identification of Endogenously Expressed Receptors in Astrocytoma Cell Lines","publication_year":2008,"publication_date":"2008-10-11","ids":{"openalex":"https://openalex.org/W2003449946","doi":"https://doi.org/10.1021/ac8016407","mag":"2003449946","pmid":"https://pubmed.ncbi.nlm.nih.gov/18847217","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2583165"},"language":"en","primary_location":{"id":"doi:10.1021/ac8016407","doi":"https://doi.org/10.1021/ac8016407","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac8016407","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2583165","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067967503","display_name":"Tomoko Kitabatake","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. Kitabatake","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]},{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011459975","display_name":"Ruin Moaddel","orcid":"https://orcid.org/0000-0002-6812-0127","observed_orcids":["https://orcid.org/0000-0002-6812-0127"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. Moaddel","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":null,"display_name":"R. Cole","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. Cole","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075461107","display_name":"M. Gandhari","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Gandhari","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067658090","display_name":"C. Frazier","orcid":"https://orcid.org/0000-0003-0838-0530","observed_orcids":["https://orcid.org/0000-0003-0838-0530"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Frazier","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069502775","display_name":"Johannes Hartenstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. Hartenstein","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039624261","display_name":"Avraham Rosenberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. Rosenberg","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075258877","display_name":"Michel Bernier","orcid":"https://orcid.org/0000-0002-5948-368X","observed_orcids":["https://orcid.org/0000-0002-5948-368X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Bernier","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073267357","display_name":"Irving W. Wainer","orcid":"https://orcid.org/0000-0003-1643-5845","observed_orcids":["https://orcid.org/0000-0003-1643-5845"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210136897","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"I. W. Wainer","raw_affiliation_strings":["Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, Maryland 21224","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5073267357"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210136897"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.5491,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.6362261,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"80","issue":"22","first_page":"8673","last_page":"8680"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ligand-gated-ion-channels","display_name":"ligand-gated ion channels","score":0.9739999771118164},{"id":"https://openalex.org/keywords/nicotinic-acetylcholine-receptors","display_name":"nicotinic acetylcholine receptors","score":0.9010000228881836},{"id":"https://openalex.org/keywords/alpha7-nachr","display_name":"alpha7 nAChR","score":0.949999988079071},{"id":"https://openalex.org/keywords/gaba-a-receptor","display_name":"GABAA receptor","score":0.8209999799728394},{"id":"https://openalex.org/keywords/nmda-receptor","display_name":"NMDA receptor","score":0.9990000128746033},{"id":"https://openalex.org/keywords/astrocytoma-cell-lines","display_name":"astrocytoma cell lines","score":0.9449999928474426},{"id":"https://openalex.org/keywords/1321n1-cells","display_name":"1321N1 cells","score":0.8989999890327454},{"id":"https://openalex.org/keywords/a172-cells","display_name":"A172 cells","score":1.0},{"id":"https://openalex.org/keywords/frontal-affinity-chromatography","display_name":"frontal affinity chromatography","score":0.7850000262260437},{"id":"https://openalex.org/keywords/epibatidine","display_name":"epibatidine","score":0.722000002861023},{"id":"https://openalex.org/keywords/bungarotoxin","display_name":"bungarotoxin","score":0.8149999976158142},{"id":"https://openalex.org/keywords/receptor-binding-affinity","display_name":"receptor binding affinity","score":0.47200000286102295}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8809823989868164},{"id":"https://openalex.org/C2780374058","wikidata":"https://www.wikidata.org/wiki/Q3333710","display_name":"Methyllycaconitine","level":5,"score":0.7957842946052551},{"id":"https://openalex.org/C2780928369","wikidata":"https://www.wikidata.org/wiki/Q423783","display_name":"Epibatidine","level":5,"score":0.7636735439300537},{"id":"https://openalex.org/C80161118","wikidata":"https://www.wikidata.org/wiki/Q408520","display_name":"Acetylcholine receptor","level":3,"score":0.6701008081436157},{"id":"https://openalex.org/C2776104367","wikidata":"https://www.wikidata.org/wiki/Q5891451","display_name":"Homomeric","level":4,"score":0.6667292714118958},{"id":"https://openalex.org/C82617044","wikidata":"https://www.wikidata.org/wiki/Q418536","display_name":"Ligand-gated ion channel","level":4,"score":0.6222907900810242},{"id":"https://openalex.org/C188987157","wikidata":"https://www.wikidata.org/wiki/Q7030581","display_name":"Nicotinic agonist","level":3,"score":0.6047661900520325},{"id":"https://openalex.org/C168258287","wikidata":"https://www.wikidata.org/wiki/Q60147","display_name":"GABAA receptor","level":3,"score":0.6019274592399597},{"id":"https://openalex.org/C50254741","wikidata":"https://www.wikidata.org/wiki/Q62536","display_name":"Ion channel","level":3,"score":0.5937077403068542},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5581774115562439},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.49889326095581055},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.4631445109844208},{"id":"https://openalex.org/C2779570518","wikidata":"https://www.wikidata.org/wiki/Q412256","display_name":"Nicotinic acetylcholine receptor","level":4,"score":0.4031340181827545},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.39980021119117737},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3425687551498413},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3373297452926636},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.2586989104747772},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D001254","descriptor_name":"Astrocytoma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008024","descriptor_name":"Ligands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011963","descriptor_name":"Receptors, GABA-A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015640","descriptor_name":"Ion Channel Gating","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015640","descriptor_name":"Ion Channel Gating","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015640","descriptor_name":"Ion Channel Gating","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015640","descriptor_name":"Ion Channel Gating","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015972","descriptor_name":"Gene Expression Regulation, Neoplastic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015972","descriptor_name":"Gene Expression Regulation, Neoplastic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015972","descriptor_name":"Gene Expression Regulation, Neoplastic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015972","descriptor_name":"Gene Expression Regulation, Neoplastic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016194","descriptor_name":"Receptors, N-Methyl-D-Aspartate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/ac8016407","doi":"https://doi.org/10.1021/ac8016407","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ac8016407","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:18847217","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18847217","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2583165","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2583165","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Anal Chem","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2583165","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2583165","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Anal Chem","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4946382540","display_name":"Immobilized Receptors In Drug Discovery","funder_award_id":"Z01AG000295","funder_id":"https://openalex.org/F4320337337","funder_display_name":"National Institute on Aging"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337337","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W288991352","https://openalex.org/W1505662983","https://openalex.org/W1581296819","https://openalex.org/W1862017790","https://openalex.org/W1970133822","https://openalex.org/W1971096666","https://openalex.org/W1972547379","https://openalex.org/W1972885484","https://openalex.org/W1980128229","https://openalex.org/W1981402797","https://openalex.org/W1982458158","https://openalex.org/W1993326575","https://openalex.org/W1994350928","https://openalex.org/W2000740344","https://openalex.org/W2015940242","https://openalex.org/W2026819406","https://openalex.org/W2032425801","https://openalex.org/W2044132060","https://openalex.org/W2051870238","https://openalex.org/W2052167215","https://openalex.org/W2054308345","https://openalex.org/W2063063986","https://openalex.org/W2069980774","https://openalex.org/W2073295417","https://openalex.org/W2116249462","https://openalex.org/W2137372598","https://openalex.org/W2168528874","https://openalex.org/W2409483866"],"related_works":["https://openalex.org/W2044132060","https://openalex.org/W2265360386","https://openalex.org/W2037012054","https://openalex.org/W1998237113","https://openalex.org/W2044472603","https://openalex.org/W2022292148","https://openalex.org/W2369837431","https://openalex.org/W1992104702","https://openalex.org/W3168855059","https://openalex.org/W2035198307"],"abstract_inverted_index":{"Cellular":[0],"membranes":[1],"obtained":[2],"from":[3],"the":[4,39,48,54,75,88,128,143,154,169,173,181],"1321N1":[5],"and":[6,26,42,45,64,83,103,115,119,121,136,145,171],"A172":[7],"astrocytoma":[8],"cell":[9],"lines":[10],"were":[11,30],"immobilized":[12],"on":[13],"a":[14],"chromatographic":[15],"phase":[16],"to":[17,87,159,166,179],"create":[18],"cellular":[19],"membrane":[20,185],"affinity":[21,34,111],"chromatography":[22,35,112],"(CMAC)":[23],"columns,":[24],"CMAC(1321N1)":[25,144],"CMAC(A172).":[27],"The":[28,50,91,125,139],"columns":[29,55,147,155],"characterized":[31],"using":[32,109,133],"frontal":[33,110],"with":[36,113],"[(3)H]-epibatidine":[37],"as":[38,47],"marker":[40],"ligand":[41],"epibatidine,":[43],"nicotine,":[44],"methyllycaconitine":[46],"displacers.":[49],"results":[51,140],"indicated":[52],"that":[53,142,153,172],"contained":[56],"homomeric":[57],"alpha7":[58],"nicotinic":[59,66],"acetylcholine":[60,67],"receptors":[61,68],"(alpha7":[62,81],"nAChR)":[63,82,86],"heteromeric":[65],"(alpha(x)beta(y)":[69,85],"nAChRs),":[70],"which":[71],"was":[72,107,131],"confirmed":[73,132],"by":[74],"addition":[76],"of":[77,93,127,163,168,183],"subtype-specific":[78],"inhibitors,":[79],"alpha-bungarotoxin":[80],"kappa-bungarotoxin":[84],"mobile":[89],"phase.":[90],"presence":[92,126],"two":[94],"additional":[95],"ligand-gated":[96],"ion":[97],"channels":[98],"(LGICs),":[99],"gamma-aminobutyric":[100],"acid":[101,105],"(GABA(A))":[102],"N-methyl-D-aspartic":[104],"(NMDA),":[106],"established":[108],"flunitrazepam":[114],"diazepam":[116],"(GABA(A)":[117],"receptor)":[118],"MK-801":[120],"NMDA":[122],"(NMDA":[123],"receptor).":[124],"four":[129,149],"LGICs":[130],"confocal":[134],"microscopy":[135],"flow":[137],"cytometry.":[138],"indicate":[141],"CMAC(A172)":[146],"contain":[148],"independently":[150],"functioning":[151],"LGICs,":[152],"can":[156,176],"be":[157,177],"used":[158,178],"characterize":[160],"binding":[161],"affinities":[162],"small":[164],"molecules":[165],"each":[167],"receptors,":[170],"CMAC":[174],"approach":[175],"probe":[180],"expression":[182],"endogenous":[184],"receptors.":[186]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2016-06-24T00:00:00"}