{"id":"https://openalex.org/W2003333228","doi":"https://doi.org/10.1210/endo-94-6-1601","title":"Properties of the Subunits of Human Chorionic Gonadotropin*","display_name":"Properties of the Subunits of Human Chorionic Gonadotropin*","publication_year":1974,"publication_date":"1974-06-01","ids":{"openalex":"https://openalex.org/W2003333228","doi":"https://doi.org/10.1210/endo-94-6-1601","mag":"2003333228","pmid":"https://pubmed.ncbi.nlm.nih.gov/4831126"},"language":"en","primary_location":{"id":"doi:10.1210/endo-94-6-1601","doi":"https://doi.org/10.1210/endo-94-6-1601","is_oa":false,"landing_page_url":"https://doi.org/10.1210/endo-94-6-1601","pdf_url":null,"source":{"id":"https://openalex.org/S157527038","display_name":"Endocrinology","issn_l":"0013-7227","issn":["0013-7227","1945-7170"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Endocrinology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044208054","display_name":"F. J. MORGANS","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F. J. MORGANS","raw_affiliation_strings":["Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111741713","display_name":"R E Canfield","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. E. CANFIELD","raw_affiliation_strings":["Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111560466","display_name":"Judith L. Vaitukaitis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. L. VAITUKAITIS","raw_affiliation_strings":["Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I78577930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113549083","display_name":"G. T. ROSS","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G. T. ROSS","raw_affiliation_strings":["Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York 10032 Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3750,"currency":"USD","value_usd":3750},"apc_paid":null,"fwci":4.669,"has_fulltext":false,"cited_by_count":73,"citation_normalized_percentile":{"value":0.94757576,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"94","issue":"6","first_page":"1601","last_page":"1606"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10960","display_name":"Hypothalamic control of reproductive hormones","score":0.329800009727478,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10960","display_name":"Hypothalamic control of reproductive hormones","score":0.329800009727478,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12587","display_name":"Gestational Trophoblastic Disease Studies","score":0.2849000096321106,"subfield":{"id":"https://openalex.org/subfields/2729","display_name":"Obstetrics and Gynecology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.13809999823570251,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-chorionic-gonadotropin","display_name":"human chorionic gonadotropin","score":0.9990000128746033},{"id":"https://openalex.org/keywords/hcg-alpha-subunit","display_name":"hCG alpha subunit","score":0.871999979019165},{"id":"https://openalex.org/keywords/hcg-beta-subunit","display_name":"hCG beta subunit","score":1.0},{"id":"https://openalex.org/keywords/subunit-dissociation","display_name":"subunit dissociation","score":0.7990000247955322},{"id":"https://openalex.org/keywords/urea-dissociation","display_name":"urea dissociation","score":0.7329999804496765},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6029999852180481},{"id":"https://openalex.org/keywords/molar-absorptivity","display_name":"molar absorptivity","score":0.7649999856948853},{"id":"https://openalex.org/keywords/bioactivity","display_name":"biological activity","score":0.46000000834465027}],"concepts":[{"id":"https://openalex.org/C163994747","wikidata":"https://www.wikidata.org/wiki/Q2916358","display_name":"Sephadex","level":3,"score":0.8709169626235962},{"id":"https://openalex.org/C2775948956","wikidata":"https://www.wikidata.org/wiki/Q407172","display_name":"Human chorionic gonadotropin","level":3,"score":0.7946529388427734},{"id":"https://openalex.org/C176685330","wikidata":"https://www.wikidata.org/wiki/Q857638","display_name":"Radioimmunoassay","level":2,"score":0.6588802337646484},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.6383990049362183},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6358457803726196},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6040178537368774},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.5734556317329407},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.4863584637641907},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4495294988155365},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4484281539916992},{"id":"https://openalex.org/C2780365088","wikidata":"https://www.wikidata.org/wiki/Q48318","display_name":"Urea","level":2,"score":0.4458339512348175},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2779359221458435},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24738022685050964},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.17083698511123657},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1058187186717987},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.10118380188941956},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006063","descriptor_name":"Chorionic Gonadotropin","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006063","descriptor_name":"Chorionic Gonadotropin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011863","descriptor_name":"Radioimmunoassay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1210/endo-94-6-1601","doi":"https://doi.org/10.1210/endo-94-6-1601","is_oa":false,"landing_page_url":"https://doi.org/10.1210/endo-94-6-1601","pdf_url":null,"source":{"id":"https://openalex.org/S157527038","display_name":"Endocrinology","issn_l":"0013-7227","issn":["0013-7227","1945-7170"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Endocrinology","raw_type":"journal-article"},{"id":"pmid:4831126","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4831126","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Endocrinology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2100798576","https://openalex.org/W2141755890","https://openalex.org/W2319730911","https://openalex.org/W599519002","https://openalex.org/W2112498819","https://openalex.org/W2066049788","https://openalex.org/W1995159148","https://openalex.org/W1841288034","https://openalex.org/W2397268294","https://openalex.org/W3025178878"],"abstract_inverted_index":{"The":[0,18,36,52],"preparation":[1],"and":[2,6,10,14,24,50,90,93,109],"properties":[3],"of":[4,38,55,63,80],"hCG":[5],"its":[7],"subunits":[8,19],"hCG\u03b1":[9,89],"hCG\u03b2":[11],"(CR115,":[12],"CR117":[13],"CR119)":[15],"are":[16,59,72,102],"described.":[17],"were":[20],"dissociated":[21],"in":[22],"urea":[23],"separated":[25],"on":[26,33],"DEAE-Sephadex":[27],"A-25":[28],"followed":[29],"by":[30,45,78],"further":[31],"purification":[32],"Sephadex":[34],"G-100.":[35],"purity":[37],"the":[39,64,81,96],"subunit":[40,57],"preparations":[41,58],"has":[42],"been":[43],"assessed":[44],"SDS-polyacrylamide":[46],"gel":[47],"electrophoresis,":[48],"bioassay,":[49],"radioimmunoassay.":[51],"biological":[53],"activities":[54],"these":[56],"less":[60,75],"than":[61,76],"1%":[62],"native":[65],"hCG.":[66],"Gel":[67],"electrophoresis":[68],"indicates":[69],"that":[70],"they":[71],"contaminated":[73],"with":[74],"2%":[77],"weight":[79],"complementary":[82],"subunit.":[83],"E":[84],"1%280nm":[85],"values":[86],"for":[87],"hCG,":[88],"hCG\u03b2)are":[91],"3.6,4.3":[92],"2.1,":[94],"respectively;":[95],"respective":[97],"molar":[98],"extinction":[99],"coefficients":[100],"(\u20ac)":[101],"1.41":[103],"\u00d7":[104,107,111],"104,":[105],"0.64":[106],"104":[108,112],"0.56":[110],"M\u22121":[113],"cm\u22121.":[114],"(Endocrinology94:":[115],"1601,":[116],"1974)":[117]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}