{"id":"https://openalex.org/W2003217869","doi":"https://doi.org/10.1046/j.1365-2583.1999.820257.x","title":"Cloning and expression of ecto 5\u2032\u2010nucleotidase from the cattle tick Boophilus microplus","display_name":"Cloning and expression of ecto 5\u2032\u2010nucleotidase from the cattle tick Boophilus microplus","publication_year":1999,"publication_date":"1999-05-01","ids":{"openalex":"https://openalex.org/W2003217869","doi":"https://doi.org/10.1046/j.1365-2583.1999.820257.x","mag":"2003217869","pmid":"https://pubmed.ncbi.nlm.nih.gov/10380109"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2583.1999.820257.x","doi":"https://doi.org/10.1046/j.1365-2583.1999.820257.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2583.1999.820257.x","pdf_url":null,"source":{"id":"https://openalex.org/S137961447","display_name":"Insect Molecular Biology","issn_l":"0962-1075","issn":["0962-1075","1365-2583"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Insect Molecular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063915590","display_name":"L. M. Field","orcid":"https://orcid.org/0000-0002-3328-2513","observed_orcids":["https://orcid.org/0000-0002-3328-2513"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L. M. Field","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039756363","display_name":"Anthony Amade James","orcid":"https://orcid.org/0000-0001-5577-3308","observed_orcids":["https://orcid.org/0000-0001-5577-3308"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. A. James","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060718309","display_name":"Nancy E. Liyou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"N. Liyou","raw_affiliation_strings":["CSIRO Tropical Agriculture, Long Pocket Laboratories, Indooroopilly, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIRO Tropical Agriculture, Long Pocket Laboratories, Indooroopilly, Queensland, Australia","institution_ids":["https://openalex.org/I1292875679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103454667","display_name":"Susan E. Hamilton","orcid":"https://orcid.org/0000-0003-1293-0777","observed_orcids":["https://orcid.org/0000-0003-1293-0777"]},"institutions":[{"id":"https://openalex.org/I165143802","display_name":"The University of Queensland","ror":"https://ror.org/00rqy9422","country_code":"AU","type":"education","lineage":["https://openalex.org/I165143802"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"S. Hamilton","raw_affiliation_strings":["Department of Biochemistry, The University of Queensland, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, The University of Queensland, Queensland, Australia","institution_ids":["https://openalex.org/I165143802"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110084497","display_name":"C. M. Elvin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"C. Elvin","raw_affiliation_strings":["CSIRO Tropical Agriculture, Long Pocket Laboratories, PMB 3, PO, Indooroopilly, Queensland 4068, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIRO Tropical Agriculture, Long Pocket Laboratories, PMB 3, PO, Indooroopilly, Queensland 4068, Australia","institution_ids":["https://openalex.org/I1292875679"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047159619","display_name":"Peter Willadsen","orcid":"https://orcid.org/0000-0003-0259-3762","observed_orcids":["https://orcid.org/0000-0003-0259-3762"]},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"P. Willadsen","raw_affiliation_strings":["CSIRO Tropical Agriculture, Long Pocket Laboratories, PMB 3, PO, Indooroopilly, Queensland 4068, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIRO Tropical Agriculture, Long Pocket Laboratories, PMB 3, PO, Indooroopilly, Queensland 4068, Australia","institution_ids":["https://openalex.org/I1292875679"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4140,"currency":"USD","value_usd":4140},"apc_paid":null,"fwci":0.4343,"has_fulltext":false,"cited_by_count":33,"citation_normalized_percentile":{"value":0.54669034,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"8","issue":"2","first_page":"257","last_page":"266"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.44530001282691956,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.44530001282691956,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.1941000074148178,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.04960000142455101,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/5-nucleotidase","display_name":"5'-nucleotidase","score":0.9279999732971191},{"id":"https://openalex.org/keywords/ecto-5-nucleotidase","display_name":"ecto-5'-nucleotidase","score":0.8669999837875366},{"id":"https://openalex.org/keywords/rhipicephalus-microplus","display_name":"Rhipicephalus microplus","score":0.9990000128746033},{"id":"https://openalex.org/keywords/cattle-ticks","display_name":"cattle ticks","score":0.9229999780654907},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.8159999847412109},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8550000190734863},{"id":"https://openalex.org/keywords/baculovirus-expression-system","display_name":"baculovirus expression system","score":0.8809999823570251},{"id":"https://openalex.org/keywords/homodimer","display_name":"homodimer","score":0.7260000109672546},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.6840000152587891},{"id":"https://openalex.org/keywords/amp-hydrolysis","display_name":"AMP hydrolysis","score":0.7950000166893005}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8485376834869385},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6380297541618347},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6241053938865662},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5841706395149231},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.525425910949707},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5181728601455688},{"id":"https://openalex.org/C2777913276","wikidata":"https://www.wikidata.org/wiki/Q24775951","display_name":"5'-nucleotidase","level":3,"score":0.46558868885040283},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4575158953666687},{"id":"https://openalex.org/C79029880","wikidata":"https://www.wikidata.org/wiki/Q901755","display_name":"GenBank","level":3,"score":0.43150535225868225},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4271647334098816},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4179793894290924},{"id":"https://openalex.org/C177707886","wikidata":"https://www.wikidata.org/wiki/Q48520","display_name":"Northern blot","level":4,"score":0.41567230224609375},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.4105633497238159}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013987","descriptor_name":"Ticks","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013987","descriptor_name":"Ticks","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015720","descriptor_name":"5'-Nucleotidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-2583.1999.820257.x","doi":"https://doi.org/10.1046/j.1365-2583.1999.820257.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1365-2583.1999.820257.x","pdf_url":null,"source":{"id":"https://openalex.org/S137961447","display_name":"Insect Molecular Biology","issn_l":"0962-1075","issn":["0962-1075","1365-2583"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Insect Molecular Biology","raw_type":"journal-article"},{"id":"pmid:10380109","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10380109","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Insect molecular biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1553288155","https://openalex.org/W2103223904","https://openalex.org/W2076817223","https://openalex.org/W1908651006","https://openalex.org/W1873866368","https://openalex.org/W3006230024","https://openalex.org/W2924455347","https://openalex.org/W1563328096","https://openalex.org/W2996849662","https://openalex.org/W1513653241"],"abstract_inverted_index":{"Although":[0],"5'-nucleotidases":[1,42],"are":[2,51],"ubiquitous":[3],"in":[4,80,85,99,105,144,148,192],"higher":[5],"vertebrates,":[6],"the":[7,18,22,40,56,60,67,120,136,149,152,180,188,197],"arthropod":[8],"enzymes":[9],"have":[10],"been":[11],"little":[12],"studied.":[13],"The":[14,33,89,101,155,177],"cDNA":[15],"sequence":[16,37],"of":[17,59,69,126,135,151,174,182],"mature":[19],"5'-nucleotidase":[20,77,130],"from":[21,131],"tick":[23,76],"Boophilus":[24],"microplus":[25],"was":[26,78,94,103,157,184],"therefore":[27],"determined":[28],"(GENBANK":[29],"accession":[30],"number:":[31],"U80634).":[32],"enzyme":[34,102,156],"has":[35],"39-41%":[36],"identity":[38],"with":[39,139,169,194],"vertebrate":[41],"and":[43,84,97,114,124,187],"contains":[44],"binuclear":[45],"metal":[46],"ion":[47],"binding":[48],"sites.":[49],"There":[50],"no":[52],"significant":[53],"introns":[54],"within":[55],"coding":[57],"region":[58],"genomic":[61],"sequence.":[62],"Southern":[63],"blot":[64],"analysis":[65],"indicates":[66],"presence":[68],"multiple":[70],"related":[71],"genes":[72],"encoding":[73],"5'-nucleotidases.":[74],"Recombinant":[75],"expressed":[79,104,153],"both":[81],"Escherichia":[82],"coli":[83,91],"baculovirus-infected":[86,106],"insect":[87,107],"cells.":[88],"E.":[90],"recombinant":[92,129],"protein":[93],"truncated,":[95],"inactive":[96],"produced":[98],"abundance.":[100],"cells":[108],"as":[109,166],"a":[110,145,167],"secreted,":[111],"soluble,":[112],"glycosylated":[113],"enzymatically":[115,127],"active":[116,128],"protein.":[117,154],"This":[118],"represents":[119],"first":[121],"successful":[122],"expression":[123],"characterization":[125],"any":[132],"organism.":[133],"Supplementation":[134],"culture":[137],"medium":[138],"25":[140],"microM":[141,186],"zinc":[142],"resulted":[143],"twofold":[146],"increase":[147],"activity":[150],"purified":[158],"to":[159],"homogeneity.":[160],"It":[161],"exists":[162],"under":[163],"non-denaturing":[164],"conditions":[165],"homodimer,":[168],"an":[170],"apparent":[171],"molecular":[172],"mass":[173],"135":[175],"kDa.":[176],"Km":[178],"for":[179,196],"hydrolysis":[181],"AMP":[183],"0.37":[185],"k(cat)":[189],"=":[190],"11.5/s,":[191],"agreement":[193],"data":[195],"native":[198],"enzyme.":[199]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}
