{"id":"https://openalex.org/W2003100658","doi":"https://doi.org/10.1021/ja906135d","title":"Identification of the ADP-Ribosylation Sites in the PARP-1 Automodification Domain: Analysis and Implications","display_name":"Identification of the ADP-Ribosylation Sites in the PARP-1 Automodification Domain: Analysis and Implications","publication_year":2009,"publication_date":"2009-09-18","ids":{"openalex":"https://openalex.org/W2003100658","doi":"https://doi.org/10.1021/ja906135d","mag":"2003100658","pmid":"https://pubmed.ncbi.nlm.nih.gov/19764761"},"language":"en","primary_location":{"id":"doi:10.1021/ja906135d","doi":"https://doi.org/10.1021/ja906135d","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja906135d","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2003100658"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016212900","display_name":"Zhihua Tao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhihua Tao","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Peng Gao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peng Gao","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084628633","display_name":"Hung\u2010wen Liu","orcid":"https://orcid.org/0000-0001-8953-4794","observed_orcids":["https://orcid.org/0000-0001-8953-4794"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hung-wen Liu","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, Division of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5084628633"],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":5.3897,"has_fulltext":false,"cited_by_count":169,"citation_normalized_percentile":{"value":0.96306517,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"131","issue":"40","first_page":"14258","last_page":"14260"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11914","display_name":"PARP inhibition in cancer therapy","score":0.9199000000953674,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11914","display_name":"PARP inhibition in cancer therapy","score":0.9199000000953674,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.05510000139474869,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/parp-1","display_name":"PARP1","score":0.9950000047683716},{"id":"https://openalex.org/keywords/poly-adp-ribosyl-ation","display_name":"poly(ADP-ribosyl)ation","score":0.8880000114440918},{"id":"https://openalex.org/keywords/tryptic-digestion","display_name":"trypsin digestion","score":0.5109999775886536},{"id":"https://openalex.org/keywords/lc-ms-ms","display_name":"LC-MS/MS","score":0.746999979019165},{"id":"https://openalex.org/keywords/synthetic-peptide-substrates","display_name":"synthetic peptide substrates","score":0.7200000286102295},{"id":"https://openalex.org/keywords/dna-damage-response","display_name":"DNA damage response","score":0.4699999988079071}],"concepts":[{"id":"https://openalex.org/C182979987","wikidata":"https://www.wikidata.org/wiki/Q3907241","display_name":"Poly ADP ribose polymerase","level":4,"score":0.9282556772232056},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7759513258934021},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.6173161864280701},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.5550795793533325},{"id":"https://openalex.org/C45510557","wikidata":"https://www.wikidata.org/wiki/Q61420","display_name":"ADP-ribosylation","level":4,"score":0.4787968099117279},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.476980984210968},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3577408194541931},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3357001841068268},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3300510048866272},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.31117865443229675},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12239101529121399}],"mesh":[{"descriptor_ui":"D000071137","descriptor_name":"Poly (ADP-Ribose) Polymerase-1","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000244","descriptor_name":"Adenosine Diphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000244","descriptor_name":"Adenosine Diphosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011065","descriptor_name":"Poly(ADP-ribose) Polymerases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011065","descriptor_name":"Poly(ADP-ribose) Polymerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011065","descriptor_name":"Poly(ADP-ribose) Polymerases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017434","descriptor_name":"Protein Structure, Tertiary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/ja906135d","doi":"https://doi.org/10.1021/ja906135d","is_oa":false,"landing_page_url":"https://doi.org/10.1021/ja906135d","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:19764761","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19764761","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W57292768","https://openalex.org/W76850629","https://openalex.org/W123340411","https://openalex.org/W1494501092","https://openalex.org/W1549011458","https://openalex.org/W1552451187","https://openalex.org/W1662942466","https://openalex.org/W1825629489","https://openalex.org/W1998596873","https://openalex.org/W2014300705","https://openalex.org/W2014312424","https://openalex.org/W2016156930","https://openalex.org/W2018329608","https://openalex.org/W2021019376","https://openalex.org/W2072528578","https://openalex.org/W2082686498","https://openalex.org/W2084291773","https://openalex.org/W2096213915","https://openalex.org/W2108979163","https://openalex.org/W2168268248","https://openalex.org/W2402426790"],"related_works":["https://openalex.org/W1992383407","https://openalex.org/W2177937990","https://openalex.org/W2084291773","https://openalex.org/W2014312424","https://openalex.org/W1605052148","https://openalex.org/W7115705144","https://openalex.org/W2332306552","https://openalex.org/W3019894137","https://openalex.org/W1575178924","https://openalex.org/W2917732060"],"abstract_inverted_index":{"Poly(ADP-ribose)":[0],"polymerase-1":[1],"(PARP-1)":[2],"is":[3,49,62,147,162,175],"a":[4,41,89],"multimodular":[5],"(domains":[6],"A,":[7],"B,":[8],"C,":[9],"D,":[10],"E,":[11],"and":[12,36,58,61,132,204],"F)":[13],"nuclear":[14],"protein":[15],"that":[16,46,143],"participates":[17],"in":[18,127,138],"many":[19],"fundamental":[20],"cellular":[21],"activities.":[22],"Stimulated":[23],"by":[24,78,116],"binding":[25],"to":[26,91,120,140,150,197],"nicked":[27],"DNA,":[28],"PARP-1":[29,57,101,146,166,169,200,214],"catalyzes":[30,106],"poly(ADP-ribosyl)ation":[31],"of":[32,71,82,110,123,145,191,213],"the":[33,64,72,79,83,93,100,111,121,188,205],"acceptor":[34],"proteins":[35],"itself":[37],"using":[38,99],"NAD(+)":[39],"as":[40,199],"substrate.":[42],"Early":[43],"studies":[44,212],"suggested":[45],"domain":[47,97,113,128,151,160,172,192],"D":[48,98,114,129,152,161,173,193],"likely":[50],"an":[51],"interface":[52],"for":[53,67,165],"protein-protein":[54],"interaction":[55],"between":[56],"its":[59],"targets":[60],"also":[63,136,195],"primary":[65],"region":[66],"automodification.":[68],"However,":[69],"determination":[70],"modification":[73,94],"sites":[74,95,126,190],"has":[75],"been":[76],"complicated":[77],"heterogeneous":[80],"nature":[81],"poly(ADP-ribose)":[84],"polymer.":[85],"Here":[86],"we":[87],"report":[88],"strategy":[90],"identify":[92],"on":[96],"E988Q":[102],"mutant,":[103],"which":[104],"only":[105],"mono(ADP-ribosyl)ation.":[107],"Trypsin":[108],"digestion":[109],"modified":[112],"followed":[115],"LC-MS/MS":[117],"analysis":[118],"led":[119],"identification":[122],"three":[124],"ADP-ribosylation":[125,189],"(D387,":[130],"E488,":[131],"E491).":[133],"Our":[134],"data":[135],"show,":[137],"contrast":[139],"early":[141],"reports,":[142],"automodification":[144],"not":[148,163],"limited":[149],"but":[153],"occurs":[154],"beyond":[155],"this":[156],"region.":[157],"In":[158],"addition,":[159],"essential":[164],"activity":[167],"since":[168],"mutant":[170],"having":[171],"deleted":[174],"still":[176],"catalytically":[177],"active.":[178],"Two":[179],"synthetic":[180],"peptides":[181],"with":[182],"amino":[183],"acid":[184],"sequences":[185],"derived":[186],"from":[187],"were":[194],"demonstrated":[196],"act":[198],"substrates.":[201],"The":[202],"methodology":[203],"results":[206],"reported":[207],"herein":[208],"will":[209],"facilitate":[210],"future":[211],"catalysis.":[215]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":14},{"year":2020,"cited_by_count":14},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":7},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":9},{"year":2014,"cited_by_count":20},{"year":2013,"cited_by_count":16},{"year":2012,"cited_by_count":8}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}