{"id":"https://openalex.org/W2003070341","doi":"https://doi.org/10.1016/s1050-3862(99)00006-6","title":"Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays","display_name":"Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays","publication_year":1999,"publication_date":"1999-12-01","ids":{"openalex":"https://openalex.org/W2003070341","doi":"https://doi.org/10.1016/s1050-3862(99)00006-6","mag":"2003070341","pmid":"https://pubmed.ncbi.nlm.nih.gov/10609756"},"language":"en","primary_location":{"id":"doi:10.1016/s1050-3862(99)00006-6","doi":"https://doi.org/10.1016/s1050-3862(99)00006-6","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s1050-3862(99)00006-6","pdf_url":null,"source":{"id":"https://openalex.org/S105549618","display_name":"Genetic Analysis Biomolecular Engineering","issn_l":"1050-3862","issn":["1050-3862","1879-1441"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetic Analysis: Biomolecular Engineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067800417","display_name":"Peter S. Nelson","orcid":"https://orcid.org/0000-0002-5451-5726","observed_orcids":["https://orcid.org/0000-0002-5451-5726"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Peter S Nelson","raw_affiliation_strings":["Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA. psnels@u.washington.edu","Department of Medicine, University of Washington, Seattle, WA 98195, USA","Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA. psnels@u.washington.edu","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Department of Medicine, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]},{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Victoria Hawkins","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Victoria Hawkins","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070447197","display_name":"Mich\u00e8l Schummer","orcid":"https://orcid.org/0000-0002-7937-7884","observed_orcids":["https://orcid.org/0000-0002-7937-7884"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michel Schummer","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015516058","display_name":"Roger E. Bumgarner","orcid":"https://orcid.org/0000-0002-8168-6985","observed_orcids":["https://orcid.org/0000-0002-8168-6985"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Roger Bumgarner","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109167898","display_name":"Wai-Lap Ng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wai-Lap Ng","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089373684","display_name":"Trey E. Ideker","orcid":"https://orcid.org/0000-0002-1708-8454","observed_orcids":["https://orcid.org/0000-0002-1708-8454"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Trey Ideker","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020752738","display_name":"Camari Ferguson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Camari Ferguson","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085306431","display_name":"Leroy Hood","orcid":"https://orcid.org/0000-0001-7158-3678","observed_orcids":["https://orcid.org/0000-0001-7158-3678","https://orcid.org/0000-0002-4157-0267"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Leroy Hood","raw_affiliation_strings":["Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biotechnology, Box 357730 HSB, Room K360, University of Washington, Seattle, WA 98195, USA","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5067800417"],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":0.76,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.66499045,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"15","issue":"6","first_page":"209","last_page":"215"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.7759000062942505,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.7759000062942505,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.11640000343322754,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0869000032544136,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/negative-selection","display_name":"negative selection","score":0.9279999732971191},{"id":"https://openalex.org/keywords/low-abundance-transcripts","display_name":"low-abundance transcripts","score":0.8500000238418579},{"id":"https://openalex.org/keywords/rare-transcripts","display_name":"rare transcripts","score":0.5509999990463257},{"id":"https://openalex.org/keywords/expressed-genes","display_name":"expressed genes","score":0.5370000004768372}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.8015717267990112},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7793543338775635},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.7435303926467896},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5510441064834595},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5092142820358276},{"id":"https://openalex.org/C13575015","wikidata":"https://www.wikidata.org/wiki/Q1562492","display_name":"Genomic library","level":4,"score":0.4887268543243408},{"id":"https://openalex.org/C81089528","wikidata":"https://www.wikidata.org/wiki/Q5134986","display_name":"clone (Java method)","level":3,"score":0.4276660680770874},{"id":"https://openalex.org/C149034497","wikidata":"https://www.wikidata.org/wiki/Q1384835","display_name":"Expressed sequence tag","level":4,"score":0.42509031295776367},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4208664298057556},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.41367653012275696},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3359363079071045},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.08922570943832397},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011467","descriptor_name":"Prostate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D015336","descriptor_name":"Molecular Probe Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020224","descriptor_name":"Expressed Sequence Tags","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/s1050-3862(99)00006-6","doi":"https://doi.org/10.1016/s1050-3862(99)00006-6","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s1050-3862(99)00006-6","pdf_url":null,"source":{"id":"https://openalex.org/S105549618","display_name":"Genetic Analysis Biomolecular Engineering","issn_l":"1050-3862","issn":["1050-3862","1879-1441"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetic Analysis: Biomolecular Engineering","raw_type":"journal-article"},{"id":"pmid:10609756","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10609756","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetic analysis : biomolecular engineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5688610956","display_name":"MOLECULAR ALTERATIONS IN PROSTATE CANCER PROGRESSION","funder_award_id":"K08CA075173","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G7025826486","display_name":null,"funder_award_id":"K08 CA75173-01A1","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W19739015","https://openalex.org/W154883909","https://openalex.org/W783423684","https://openalex.org/W1865508414","https://openalex.org/W1970156673","https://openalex.org/W1997681444","https://openalex.org/W1998415512","https://openalex.org/W1999866212","https://openalex.org/W2014195831","https://openalex.org/W2018994790","https://openalex.org/W2021131806","https://openalex.org/W2029929383","https://openalex.org/W2055043387","https://openalex.org/W2064013653","https://openalex.org/W2069271664","https://openalex.org/W2072069835","https://openalex.org/W2074242397","https://openalex.org/W2084637809","https://openalex.org/W2094448442","https://openalex.org/W2094576801","https://openalex.org/W2101455212","https://openalex.org/W2138168728","https://openalex.org/W2151238743","https://openalex.org/W4249877820"],"related_works":["https://openalex.org/W1800372423","https://openalex.org/W160690100","https://openalex.org/W7096830322","https://openalex.org/W1998415512","https://openalex.org/W2101455212","https://openalex.org/W58989343","https://openalex.org/W2020996918","https://openalex.org/W1599365594","https://openalex.org/W3021772666","https://openalex.org/W23700819"],"abstract_inverted_index":null,"counts_by_year":[{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}