{"id":"https://openalex.org/W2002725152","doi":"https://doi.org/10.4155/pbp.13.56","title":"The use of glutamine synthetase as a selection marker: recent advances in Chinese hamster ovary cell line generation processes","display_name":"The use of glutamine synthetase as a selection marker: recent advances in Chinese hamster ovary cell line generation processes","publication_year":2013,"publication_date":"2013-12-01","ids":{"openalex":"https://openalex.org/W2002725152","doi":"https://doi.org/10.4155/pbp.13.56","mag":"2002725152"},"language":"en","primary_location":{"id":"doi:10.4155/pbp.13.56","doi":"https://doi.org/10.4155/pbp.13.56","is_oa":false,"landing_page_url":"https://doi.org/10.4155/pbp.13.56","pdf_url":null,"source":{"id":"https://openalex.org/S2764602892","display_name":"Pharmaceutical Bioprocessing","issn_l":"2048-9145","issn":["2048-9145","2048-9153"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320466","host_organization_name":"OMICS Publishing Group","host_organization_lineage":["https://openalex.org/P4310320466"],"host_organization_lineage_names":["OMICS Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pharmaceutical Bioprocessing","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060938094","display_name":"Lianchun Fan","orcid":"https://orcid.org/0000-0002-6312-4823","observed_orcids":["https://orcid.org/0000-0002-6312-4823"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lianchun Fan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110908447","display_name":"Christopher C. Frye","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Christopher C Frye","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5075454956","display_name":"Andrew J. Racher","orcid":"https://orcid.org/0000-0003-2546-1228","observed_orcids":["https://orcid.org/0000-0003-2546-1228"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Andrew J Racher","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3299,"has_fulltext":false,"cited_by_count":38,"citation_normalized_percentile":{"value":0.53562444,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"1","issue":"5","first_page":"487","last_page":"502"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.009200000204145908,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutamine-synthetase","display_name":"glutamine synthetase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/selectable-marker","display_name":"selectable marker","score":0.8679999709129333},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.8939999938011169},{"id":"https://openalex.org/keywords/cell-line-establishment","display_name":"cell line establishment","score":0.9179999828338623},{"id":"https://openalex.org/keywords/monoclonal-antibody-production","display_name":"monoclonal antibody production","score":0.8220000267028809},{"id":"https://openalex.org/keywords/clonal-cell-lines","display_name":"clonal cell lines","score":0.6470000147819519},{"id":"https://openalex.org/keywords/biopharmaceutical-manufacturing","display_name":"biopharmaceutical manufacturing","score":0.7630000114440918},{"id":"https://openalex.org/keywords/antibody-humanization","display_name":"antibody humanization","score":0.6679999828338623},{"id":"https://openalex.org/keywords/biosimilars","display_name":"biosimilars","score":0.7210000157356262}],"concepts":[{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.7361885905265808},{"id":"https://openalex.org/C59491497","wikidata":"https://www.wikidata.org/wiki/Q864715","display_name":"Biosimilar","level":2,"score":0.7032884359359741},{"id":"https://openalex.org/C2775893736","wikidata":"https://www.wikidata.org/wiki/Q25324110","display_name":"Biomanufacturing","level":2,"score":0.6529912352561951},{"id":"https://openalex.org/C2780868878","wikidata":"https://www.wikidata.org/wiki/Q1660197","display_name":"Immunogenicity","level":3,"score":0.49000540375709534},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4840187728404999},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.45641568303108215},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.448037326335907},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3778260350227356},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.36517852544784546},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36285436153411865},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.36263322830200195},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.23199787735939026},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.15460455417633057},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.15043216943740845},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10727229714393616}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4155/pbp.13.56","doi":"https://doi.org/10.4155/pbp.13.56","is_oa":false,"landing_page_url":"https://doi.org/10.4155/pbp.13.56","pdf_url":null,"source":{"id":"https://openalex.org/S2764602892","display_name":"Pharmaceutical Bioprocessing","issn_l":"2048-9145","issn":["2048-9145","2048-9153"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320466","host_organization_name":"OMICS Publishing Group","host_organization_lineage":["https://openalex.org/P4310320466"],"host_organization_lineage_names":["OMICS Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pharmaceutical Bioprocessing","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":124,"referenced_works":["https://openalex.org/W32934706","https://openalex.org/W73351523","https://openalex.org/W95207216","https://openalex.org/W188944471","https://openalex.org/W203131494","https://openalex.org/W337776191","https://openalex.org/W650801401","https://openalex.org/W650959505","https://openalex.org/W1188217804","https://openalex.org/W1220980554","https://openalex.org/W1498699116","https://openalex.org/W1504978712","https://openalex.org/W1516308718","https://openalex.org/W1530880785","https://openalex.org/W1541218827","https://openalex.org/W1595400617","https://openalex.org/W1805618930","https://openalex.org/W1886156337","https://openalex.org/W1929540365","https://openalex.org/W1964525381","https://openalex.org/W1965177712","https://openalex.org/W1966603679","https://openalex.org/W1967046337","https://openalex.org/W1967142377","https://openalex.org/W1972584860","https://openalex.org/W1974701232","https://openalex.org/W1974887619","https://openalex.org/W1979578972","https://openalex.org/W1982609556","https://openalex.org/W1983163757","https://openalex.org/W1983182864","https://openalex.org/W1987042073","https://openalex.org/W1988836643","https://openalex.org/W1991344098","https://openalex.org/W1997289584","https://openalex.org/W2000118992","https://openalex.org/W2000213886","https://openalex.org/W2002029926","https://openalex.org/W2002635086","https://openalex.org/W2004360848","https://openalex.org/W2006554573","https://openalex.org/W2008553597","https://openalex.org/W2008907048","https://openalex.org/W2012144638","https://openalex.org/W2012972704","https://openalex.org/W2014560560","https://openalex.org/W2015872305","https://openalex.org/W2016613538","https://openalex.org/W2020916353","https://openalex.org/W2021402286","https://openalex.org/W2022237684","https://openalex.org/W2022405729","https://openalex.org/W2027783748","https://openalex.org/W2028317590","https://openalex.org/W2029545026","https://openalex.org/W2029867298","https://openalex.org/W2029878671","https://openalex.org/W2030932286","https://openalex.org/W2033433083","https://openalex.org/W2037141508","https://openalex.org/W2038613649","https://openalex.org/W2042059381","https://openalex.org/W2043734426","https://openalex.org/W2048162805","https://openalex.org/W2048588519","https://openalex.org/W2048941695","https://openalex.org/W2051395442","https://openalex.org/W2053953032","https://openalex.org/W2056368762","https://openalex.org/W2058061694","https://openalex.org/W2058198081","https://openalex.org/W2059987855","https://openalex.org/W2062438194","https://openalex.org/W2065876190","https://openalex.org/W2071702521","https://openalex.org/W2071919990","https://openalex.org/W2076083204","https://openalex.org/W2076595618","https://openalex.org/W2076918952","https://openalex.org/W2077439937","https://openalex.org/W2078561983","https://openalex.org/W2078893885","https://openalex.org/W2086608701","https://openalex.org/W2088987512","https://openalex.org/W2090641782","https://openalex.org/W2091713101","https://openalex.org/W2094194370","https://openalex.org/W2096791413","https://openalex.org/W2099841900","https://openalex.org/W2105908081","https://openalex.org/W2106785697","https://openalex.org/W2108422551","https://openalex.org/W2109666318","https://openalex.org/W2109666766","https://openalex.org/W2112444085","https://openalex.org/W2113446079","https://openalex.org/W2118540273","https://openalex.org/W2121074166","https://openalex.org/W2123502778","https://openalex.org/W2123746851","https://openalex.org/W2127582347","https://openalex.org/W2130008544","https://openalex.org/W2132835600","https://openalex.org/W2133003674","https://openalex.org/W2133385816","https://openalex.org/W2133513884","https://openalex.org/W2135849966","https://openalex.org/W2140035322","https://openalex.org/W2141361924","https://openalex.org/W2146479955","https://openalex.org/W2146774140","https://openalex.org/W2151722718","https://openalex.org/W2161463357","https://openalex.org/W2162005401","https://openalex.org/W2167462535","https://openalex.org/W2168072557","https://openalex.org/W2168460045","https://openalex.org/W2169193374","https://openalex.org/W2254975776","https://openalex.org/W2405887519","https://openalex.org/W2431756740","https://openalex.org/W2522830001","https://openalex.org/W4233249586","https://openalex.org/W4388386370"],"related_works":["https://openalex.org/W2942827659","https://openalex.org/W3210031427","https://openalex.org/W4401369340","https://openalex.org/W7205708334","https://openalex.org/W4243803935","https://openalex.org/W2797243546","https://openalex.org/W2209949284","https://openalex.org/W2080652213","https://openalex.org/W4416356065","https://openalex.org/W2142587870"],"abstract_inverted_index":{"Central":[0],"to":[1,8,76,113,135],"the":[2,5,10,87,101,123,128,131,137],"needs":[3],"of":[4,12,36,66,130],"biopharmaceutical":[6],"industry":[7],"support":[9],"development":[11],"innovative":[13],"biologics":[14],"and":[15,20,47,56,117,120],"biosimilars":[16],"are":[17],"more":[18],"effective":[19],"efficient":[21],"manufacturing":[22],"processes":[23,89],"which":[24],"require":[25],"highly":[26],"productive":[27],"cell":[28,50,71,92,107],"lines":[29],"with":[30,69],"desired":[31],"quality":[32],"attributes.":[33],"New":[34],"designs":[35],"molecules":[37],"such":[38],"as":[39],"antibody":[40,62],"humanization":[41],"have":[42,59,95],"greatly":[43],"reduced":[44],"immunogenicity":[45],"concerns,":[46],"advances":[48],"in":[49,64,73,100],"culture":[51],"technology":[52],"including":[53,142],"media":[54],"optimization":[55],"process":[57],"control":[58],"driven":[60],"monoclonal":[61],"productivities":[63],"excess":[65],"10":[67],"g/l":[68],"peak":[70],"densities":[72],"bioreactors":[74],"climbing":[75],"over":[77,83],"35":[78],"million":[79],"per":[80],"ml.":[81],"However,":[82],"this":[84],"same":[85],"timeframe,":[86],"fundamental":[88],"utilized":[90],"for":[91,104,127],"line":[93,110],"generation":[94,111],"not":[96],"changed":[97],"significantly,":[98],"especially":[99],"selection":[102],"step":[103,126],"top-producing":[105],"clonal":[106],"lines.":[108],"Cell":[109],"continues":[112],"be":[114],"time":[115],"consuming":[116],"labor":[118],"intensive":[119],"has":[121],"become":[122],"timeline":[124],"limiting":[125],"majority":[129],"industry.":[132],"In":[133],"order":[134],"meet":[136],"\u2018Fast-to-Proof-of-Principle\u2019":[138],"strategy,":[139],"multiple":[140],"efforts":[141],"...":[143]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}